• Journal of Embryo Transfer
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• v.22 no.2
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• pp.89-95
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• 2007

The present study was conducted to examine some factors affecting in vitro development and fecundity of embryos recloned with somatic cell nuclear transfer (SCNT). Fibroblast cells retrieved from the ear of a 3-week-old, cloned Korean goat (Jinsoonny) were used as karyoplast donors and serum-starvation was conducted in tissue culture medium (TCM)-199 supplemented with 0.5% FBS. Recipient oocytes were surgically collected by flushing the oviducts 35 h after hCG injection following FSH priming. The zonae pellucidae of the oocytes were partially perforated with a laser drill and a donor cell was transferred into an enucleated oocyte. The couplets were electrically fused and activated by ionomycin (5 min) and 6-DMAP (4 h). The reconstructed embryos were cultured in mSOF medium containing 0.8% BSA at $39^{\circ}C$ in an atmosphere of 5% $CO_2$, 5% $%O_2$, 90% $N_2$ for 12 to 15 h. Re-cloned embryos (2- to 4-cell stages) were surgically transferred into the oviducts of the recipients and pregnancy was subsequently diagnosed by progesterone assay and ultrasound on Days 21 and 63 of pregnancy. The fusion rate following 1st fusion pulse was higher (p<0.05) in 2nd cloning (65.9%) compared to 1st cloning (51.0%), but it was not different in the other groups. The rate of cleavage after fusion was significantly higher (p<0.05) in 1st (77.7%) than in 2nd cloning (56.0%). A total of 175 re-cloned embryos were transferred into 28 recipients. On day 21 and 60 after transfer, 11 (39.3%) and 4 recipients (17.4%) were pregnancy, respectively. In comparison of pregnancy rate by estrous synchronization, a total of 66 and 109 re-cloned embryos were transferred into 11 recipients in natural estrus and 17 recipients in induced estrus, respectively. Five (45.4%) and 2 recipients (18.2%) in natural estrus were pregnant on days 21 and 63 while 6 (35.3%) and 2 (11.8%) recipients in induced estrus were pregnant, respectively. These results show that recloning of goat can be achieved by SCNT and estrous synchronization between donor and recipient animals may be one of the major factors affecting success rate.

• The Journal of Korean Physical Therapy
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• v.13 no.2
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• pp.399-405
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• 2001

Attainment of the goals of functional electrical stimulation(FES) applications such as strengthening of muscle, increasing muscular endurance, improvement in joint range of motion or the reduction of spasticity does not insure that patients will be able to produce voluntary muscle contraction sufficient to maintain posture or produce purposeful movements. In many patients who have sustained CNS damage, the control exerted by higher nervous system center over muscle contraction may impaired. In Searched patients, a variety of advanced therapeutic exercise techniques have been employed traditionally to facilitate the return of controlled functional muscular activity or maintain postural alignment until recovery from dysfunction occurs Among the most common clinical applications of FES for functional muscle contraction is as a substitute for static or dynamic orthotic devices

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2004.10a
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• pp.1137-1142
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• 2004

Walking training is one of the most important rehabilitation processes with paralysis patient. Walking training by using an orthosis can help advancing a patient's independent level. However, existing orthoses have some serious demerit of mechanical problem that the knee joint is locked in the state where it is completely extended, which increases energy consumption and fatigue. For this reason, it is suggested, for more practical orthosis, that the knee joint should be placed and it should have capability of suspending patient's weight. In this paper, 1-DOF walking orthosis which compensates the demerit of the existing orthosis and secures patient's mobility has been proposed. New orthosis has been designed under the following two premises. First, the knee joint of the orthosis was designed fold in order for the orthosis to move in a walking pattern similar to that of a normal person. Second, the knee joint was designed to extend during the swing phase and lock safely during the stance phase.

• Journal of Biomedical Engineering Research
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• v.14 no.4
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• pp.349-354
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• 1993

To investigate the relations between the physical constitutions, the acid-base balance, and the autonomic nervous function, the blood gas, the heart rate and the concentrations of Na, K of serum were measured pre-and poststimulation. As the result, it is shown that in the normal condition the macro-negative(strong liver- weak lung type) is the metabolic alkalosis, the macro-positive (strong lung-weak liver type) is the metabolic acidosis, the micro-negative(strong kidney-weak spleen type) is the alkalosis and the micro-positive (strong spleen-weak kidney type) is the acidosis. And the ntacro-positive and micro-positive are the sympathetic lead, the macro-negative and micro-negative are the parasympathetic lead. In the macro-positive and micro-positive subjects, the concentration of K in serum is relatively high. This si coincided with the acidosis. But in the macro-negative and micro-negative subjects, the concentration of K in serum is relatively low. This is coincided with the alkalosis.

• Proceedings of the KOSOMBE Conference
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• v.1994 no.05
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• pp.88-91
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• 1994

We develope a assistant system of foot pressure distribution for improvement gait Pattern, adapted working speed, and minimitation of muscle fatigue of the sensory feedback type FES system(SEFES). This measurement system consist of mat type pressure sensor with piezo electric films. The pressure data signal multiflexed input scanning method processed A/D conversion after two step amplify and integrate. Matrix sensor interface to PC for pseudo color display by level of Pressure distribution data. This measurement system clinically evaluated in hemiplegic patients. It has produced acceptable results with optimal location of the food sensor's pressure point and avoid the muscle fatigue.

• Journal of Biomedical Engineering Research
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• v.38 no.4
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• pp.190-196
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• 2017

This study aims to investigate how the game based FES paradigm influences on EEG spectrum. Thirteen subjects were recruited for this study. We selected Fp1, Fp2, F3, F4, T3, T4, P3 and P4 for the EEG recording and the wrist was selected as the FES rehabilitation site. EEG spectrum were compared between FES only and FES with game condition. The results showed that FES game significantly suppressed alpha rhythm in EEG compared to the FES only condition.

• The Korean Journal of Pain
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• v.3 no.1
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• pp.55-58
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• 1990

A case of severe complications following intrathecal administration of 45 ml of hypertonic saline solution for the treatment of postherpetic neuralgia was presented. Transient immediate complications included were tachycardia, hypertension, neck stiffness and muscle twitch. Pulmonary edema, paralytic intestinal obstruction, and the cauda equina syndrome including sphincter disorder with atonic urinary bladder developed shortly after the injection. Tenesmus and sensory abnormality around perineum and soles were the longlasting complications.

• 제어로봇시스템학회:학술대회논문집
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• 1992.10a
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• pp.90-94
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• 1992

A portable multichannel functional electrical stimulation(FES) system for the fine control of the paralyzed extremities in spinal cord injury patients is described. This system is composed of a stimulation data creating system, a serial communication device, a 16-bit microprocessor, D/A converter of 32 channels and a display device. Stimulation patterns are created from analytical results of integrated EMGs during motion in normal subjects and are stored in the stimulation data creating system as data files. And then the stimulation patterns are sent to the memory in the portable multichannel FES system through serial communication interfacing device. Sophisticated fine control of paralyzed extrimities was realized by transmitting multichannel stimulation patterns to percutaneous intramuscular electrodes, which stimulate the motor function of paralyzed muscle simultaneously. Advantages of this system are as follws: 1) It is possible to modify stimulation patterns in accordance with the patient's situation. 2) This system is small and light.

• Journal of Yeungnam Medical Science
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• v.24 no.2
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• pp.97-106
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• 2007

Functional electrical stimulation (FES) has developed over the last 35 years to become a scientifically, technologically and clinically recognized field of interest in clinical medicine. FES has been applied to locomotion, grasping, ventilation, incontinence, and decubitus healing. However, all of these achievements illustrate the initial applications of FES; its true potential has not yet been realized. Recently, FES systems, which are miniaturized stimulation devices, have been utilized in the clinical setting. However, because the stimulating electrodes of the current FES devices are percutaneous electrodes, which are susceptible to wire breakage, and skin infection an implantable FES stimulating electrode has been introduced in the U.S. and Japan. In the present study, an external power supply method using radio frequency (RF) coupling and data transmission was developed for the control of the implantable FES device. In addition, we review the current understanding of FES devices and their application in clinical medicine.

• Journal of the Korean Institute of Intelligent Systems
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• v.10 no.4
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• pp.304-309
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• 2000

For understanding the information processing in human brain, we analyze the EEG, a spontaneous electric activity on the scalp of the human. In this paper, we used the mutual information to analyze EEG. The mutual information is used to show the stochastic correlation between signals which are generated in the communication and information theory. The used EEG is evoked by each auditory stimulus in positive and negative emotional states. As a result, we found thet there is some difference at the mutual information in each emotional state.