• Title/Summary/Keyword: 적혈구 응집

Search Result 89, Processing Time 0.021 seconds

Trypsin Inhibitor and Hemagglutinating Activities of Some Minor Beans in Korea (한국산 두류(頭類)의 Trypsin 저해(沮害) 활성(活性) 및 적혈구(赤血球) 응집(凝集) 활성(活性))

  • Kang, Myung-Hee;Kim, Yong-Hwa;Lee, Su-Rae
    • Korean Journal of Food Science and Technology
    • /
    • v.12 no.1
    • /
    • pp.24-33
    • /
    • 1980
  • Trypsin inhibitor and hemagglutinating activities of some minor beans produced in Korea were determined in comparison with those of soybean and the effects of heat treatment on the activities were studied. The results are summarized as follows 1. The trypsin inhibitor activity (% [TU] inhibited/mg) of soybean, red bean, kidney bean, and mung bean were 79.9, 46.4, 43.2 and 17.7, respectively, on the dry weight basis and were 194, 222, 170 and 75, respectively, on the protein basis. 2. Heat destruction by boiling or autoclaving of trypsin inhibitor activity of red bean, mung bean, kidney bean, and soybean were $85{\sim}87,\;87{\sim}94,\;76{\sim}79\;and\;67{\sim}72\;%$, respectively. No significant difference was, however, observed in the effect between the two heating methods. 3. The hemagglutinating activity (unit/g) of kidney bean, soybean, mung bean and red bean were 48,300, 18,000, 136 and non-detectable, respectively, on the dry weight basis and were 190,600, 43,700, 581 and non-detectable, respectively, on the protein basis. Heat treatment destructed the hemagglutinating activity in all three beans.

  • PDF

Canine Immune-Mediated Hemolytic Anemia (개의 면역성 용혈성 빈혈)

  • 보니안빈셀만-남;이현범
    • Journal of Veterinary Clinics
    • /
    • v.13 no.1
    • /
    • pp.96-101
    • /
    • 1996
  • 면역성 용혈성 빈혈증 병견은 임상적으로 허약, 황달, 발열, 침울 및 점막창백을 나타낸다. 본 병의 진단은 구상적혈구증가증, 혈구응집반응 또는 직접적 Coomb시험 양성반응을 확인함과 동시에 용혈성 빈혈의 다른 원인을 배제함으로서 확정하여야 한다. 치료방법에는 적절한 보조적 요법과 함께 면역억압제가 포함된다. 일반적으로 치료에 있어서는 일차적으로 glucocorticoids가 선택되는데 흔히 cytoxan, aziothioprin, vincristine 또는 danazole과 같은 다른 약제와 병용된다. 치료는 그 반응에 근거하여 면역억압제의 용량을 2-4주 간격으로 점차 감량하면서 6개월 또는 그 이상까지 계속한다. 면역억압제에 대한 치료반응은 지연될 수 있고 또 적혈구 보유가 골수로부터 새로운 적혈구를 유리하게될 때까지 병축을 유지시키기에 부적당할 수 있기 때문에 병축의 예후판정은 경계하여야 한다. 심급성 내지 급성 예는 일반적으로 예후가 보다더 좋지 않다.

  • PDF

Forecasting of erythrocyte sedimentation rate using gated recurrent unit (GRU) neural network (Gated recurrent unit (GRU) 신경망을 이용한 적혈구 침강속도 예측)

  • Lee, Jaejin;Hong, Hyeonji;Song, Jae Min;Yeom, Eunseop
    • Journal of the Korean Society of Visualization
    • /
    • v.19 no.1
    • /
    • pp.57-61
    • /
    • 2021
  • In order to determine erythrocyte sedimentation rate (ESR) indicating acute phase inflammation, a Westergren method has been widely used because it is cheap and easy to be implemented. However, the Westergren method requires quite a long time for 1 hour. In this study, a gated recurrent unit (GRU) neural network was used to reduce measurement time of ESR evaluation. The sedimentation sequences of the erythrocytes were acquired by the camera and data processed through image processing were used as an input data into the neural network models. The performance of a proposed models was evaluated based on mean absolute error. The results show that GRU model provides best accurate prediction than others within 30 minutes.

Studies on the Tuberculin - 2) Serological observation of hemagglutinating antibody in guinea pigs sensitized with heat killed tubercle bacilli (Tuberculin에 관한 연구(硏究) - (2) 항원감작적혈구응집반응(抗原感作赤血球凝集反應)에 의한 결핵사균면역(結核死菌免疫) Guinea pig 혈제(血濟)의 적혈구응집(赤血球凝集) 항체가(抗體價) 소장(消長))

  • Kim, Jung-Kyu
    • The Journal of the Korean Society for Microbiology
    • /
    • v.5 no.1
    • /
    • pp.41-48
    • /
    • 1970
  • In order to assay one lot of the potency of tuberculin for intradermic use, four guinea pigs sensitized with heat killed M. tuberculosis were used by the method originally of the Agriculture Research Service Center(A. R. S.) and Bureau of Animal. Industry(B. A. I.) in the United. States of America in which guinea pigs were used on 21st day after sensitization. There is doubt whether the guinea pigs are sensitized or not. Therefore, this study has taken the method of Middle Brook-Dubos hemagglutination test, and observed the humoral hemagglutinating antibody in the blood. The results obtained are as follows. 1. The maximum titer of hemagglutinating antibody was demonstrated 30days after sensitizing the guinea pigs with killed tubercle bacilli. The maximum titer was continued for a period of 45 days and thereafter declined gradually. 2. The swelling and redness of the region of the sensitized guinea pigs was shown to be reached to the maximum size within 24 hours after intradermal inoculation with HCSM tuberculin and gradually reduced 96 hours after inoculation. The swelling and redness could not be detected by macro-observation after 14 days.

  • PDF

Studies on Antigencities of Sperm and Seminal Plasma, and Effects of Their Antibodies on Fertilization in Rabbit I. Antigenicities of sperm and seminal plasma (가토에 있어서 정자 및 정장의 항원성과 이의 항체가 수정에 미치는 영향 I. 정자 및 정장의 항원성)

  • 이용우;김창근;정영채;서경덕
    • Korean Journal of Animal Reproduction
    • /
    • v.11 no.1
    • /
    • pp.42-62
    • /
    • 1987
  • 본 실험은 성숙가토의 정자와 정장의 항원성을 조사하여 면역적 불임원인 규명에 필요한 기초자료를 얻고자 시도하였다. 인공질로 채취한 정액을 원심분리하여 얻은 정자와 정장을 항원으로 사용하였으며 항원성의 측정방법은 크로마토그라프에 의한 단백질 분리, SDS-PAGE, HPLC, 한천확산법, 전기영동, 수동적혈구응집방법 및 부동화시험이었다. 얻어진 결과는 다음과 같다. 1. SDS-PAGE 전기영동시 정상가토 정장에서 약 23개의 단백질이 분리되었고, 그중 분자량이 약 20,000되는 단백질부분이 정관절제 수술한 정장에서 나타나지 않았다. 2. 정상가토 정장을 HPLC를 이용한 분석에서 3개의 peaks을 볼 수 있었고, 정관절제수술한 것에서는 peak 1에 해당하는 단백질이 소실되었다. 3. 한천확산시험에서 정상정장은 이종혈청과는 4개의 침강선을 나타냈고, 전기영동에서는 7개의 침강선을 나타냈다. 4. 이종면역에서 정자 및 정장은 항체가 상승이 용이하였지만, 동종면역시는 추가면역이 필요하였으며 개체간의 역가차이를 보였다. 정자면역한 자성가토에서는 수동적혈구응집반응을 나타냈지만, 한천확산 및 전기영동반응은 보이지 않았고, 같은 처치를 받은 웅성가토에서도 역시 같은 반응양상을 나타냈다. 5. 동종 및 이종항혈청을 이용한 교차전기영동방법으로 정장의 항원적 구성요소를 구별할 수 있었다. 사출된 정액에서 분리된 정장은 일부 항원을 포함하고 있었으며 이는 성숙한 정자에서 기인한 것으로 사료되었다.

  • PDF

Biochemical Characterization of Lectin Isolated from Cherry Tomato Fruit (방울토마토 열매로부터 분리된 lectin의 생화학적 특성)

  • Park, Na-Young;Lee, Sam-Pin;Roh, Kwang-Soo
    • Journal of Life Science
    • /
    • v.17 no.2 s.82
    • /
    • pp.254-259
    • /
    • 2007
  • Biochemical characterization of lectin isolated from fruit of cherry tomato through neutral saline extraction, ammonium sulfate precipitation, and affinity chromatography on Sephadex G-200 was studied. The lectin was agglutinated by trypsin-treated human ABO erythrocytes, and the most pronounced activity of agglutination was observed at B type erythrocyte. The analysis of the lectin by SDS-PAGE showed the high intensity band with molecular weights of 10.7 kDa. The optimal temperature and thermal stability of the lectin was $40^{\circ}C$ and $40-60^{\circ}C$, respectively. The maximal pH of this lectin was pH 7.2.

Analysis and Characterization of the Taro (colocasia antiquorum) lsolectin (토란 Isolectin의 분석 및 특성)

  • 서영주;삼호정만
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.23 no.2
    • /
    • pp.308-314
    • /
    • 1994
  • Four Taro isolectins (I, II, III, IV) were purified by ammonium sulfate, chromatography on CM-celluose and isoelectric focusing. I and IV lectins proved homogeneous by disk polyacrylamid gel electrophoresis and densitometric patterns. But in the presence of urea, IV lectin further dissociated into two different subunits. These lectinis had different hemagglutinating activities and inhibition in their activities after mixed with pepsin particuclary, but not with carbohydrates, heating pH, urea, guanidine, trypsin, pronase and $Ca^{2+}$.

  • PDF

Anti-coagulation and Anti-platelet Aggregation Activities of Black Ginger (Kaempferia parviflora) (흑생강(Kaempferia parviflora)의 항응고 및 혈소판 응집저해 활성)

  • Lee, Man-Hyo;Sung, Hwa-Jung;Kwon, Chong Suk;Sohn, Ho-Yong
    • Journal of Life Science
    • /
    • v.28 no.9
    • /
    • pp.1068-1075
    • /
    • 2018
  • Kaempferia parviflora, an herbaceous plant in the family Zingiberaceae, is popular in many tropical regions. It is called as black ginger or krachaidum in Thailand and Laos, and its raw or dried root have been used as spices and teas. The rhizomes also have been traditionally used to treat gastrointestinal disorders, ulcers, gout, dysentery, allergies and to improve physical work capacity. Recently, its anti-obesity, anti-oxidant, anti-inflammatory and blood clot-lysis activities were reported. In this study, the anti-thrombosis activity of black ginger was investigated, since improvement in blood fluidity leads to the prevention of various lifestyle-related diseases. The hot water and ethanol extract and their subsequent solvent fractions (hexane, ethylacetate, butanol fractions and water residue) were prepared, and their anti-coagulation and platelet aggregation inhibitory activities were determined, respectively. Among the black ginger extracts and their fractions, the ethylacetate fraction (EAF) of ethanol extract only showed significant extensions of blood coagulation time determined by thrombin time (TT), prothrombin time (PT), and activated partial thromboplastin time (aPTT). At 5 mg/ml concentration, TT, PT and aPTT were extended to 1.22, 1.49 and >15-folds compared to non-treatment. The EAFs of ethanol and hot water extract showed strong inhibitions against collagen-induced platelet aggregations, which are comparable to inhibitions of aspirin. Also the EAFs from black ginger did not show any hemolysis activity against human RBC up to 0.5 mg/ml. Our results suggest that the EAF of black ginger has a potential as novel anti-coagulation and ant-platelet aggregation agent. This report provides the first evidence of anti-coagulation activity of black ginger.

An Evaluation of the Anti-thrombosis and Antioxidant Activities of Different Parts of Dystaenia takeshimana (섬바디 부위별 추출물의 항혈전 및 항산화 활성)

  • Kang, Deok-Gyeong;Lee, Yun-Jin;Kim, Jong Sik;Sohn, Ho-Yong
    • Journal of Life Science
    • /
    • v.32 no.4
    • /
    • pp.303-309
    • /
    • 2022
  • Dystaenia takesimana is an endemic plant found only in Korea, especially on Ulleung Island. The leaves and roots of D. takesimana have been used as food, forage, and oriental medicine. Anti-bacterial, anti-inflammation, antioxidant, and α-glucosidase inhibition biological activities have been reported in the plant's root extract. However, studies concerning the anti-thrombosis activities of D. takesimana are still in the rudimentary stage. In this study, the extracts of the leaf (DT-L), stem (DT-S), and root (DT-R) of D. takesimana were prepared using 70% ethanol, and their anti-thrombosis activities were evaluated. DT-L extracts (0.25 mg/ml) showed strong inhibitions against platelet aggregation, comparable to aspirin, with strong radical scavenging activities. Furthermore, the DT-L extract did not show any RBC hemolysis up to 1 m/ml. The ant-coagulation and antioxidant activities of the DT-S extract were ignorable. While the DT-R extract showed inhibitions against thrombin and blood coagulation factors, it also showed strong platelet aggregation. This is a first report of the anti-thrombosis activities of D. takesimana, and our results suggest that DT-L could be developed as a valuable bioresource for high value-added products.