• Title/Summary/Keyword: 저항성 탐색

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Identification of novel genes for improvement of downy mildew resistance in Zea mays (옥수수의 노균병 저항성 증대를 위한 저항성 유용유전자 발굴)

  • Min, Kyeong Do;Kim, Hyo Chul;Kim, Kyung-Hee;Moon, Jun-Cheol;Lee, Byung-Moo;Kim, Jae Yoon
    • Korean Journal of Environmental Biology
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    • v.37 no.4
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    • pp.493-502
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    • 2019
  • Maize (Zea mays L.) is a C4-plant and one of the three major crops grown worldwide. Because of its high productivity, maize is considered as one of the most important food and feed stocks in the world. Recently, bioethanol from maize was predominantly generated in the USA and Brazil. Infection of maize by several diseases resulted in a huge disaster and prevented maize production. Downy mildew, caused by Peronosclerospora sorghi, is one of the most serious diseases of maize. Despite efforts to develop downy mildew-resistant cultivars or seed treatment with metalaxyl, downy mildew persists as a serious pathogen and is still prevalent in specific geographical locations. Analysis of soils infected with downy mildew and investigation of candidates associated with downy mildew resistance is an attractive method to overcome downy mildew damage in maize. In a previous study, we reported that maize chromosome 6 carries a possible candidate gene for downy mildew resistance. Using bioinformatics tools and RT-PCR analysis, five novel genes including bZIP, OFP transcription factor, and Ppr were identified as candidate genes associated with downy mildew resistance.

Identification of Quantitative Trait Loci Associated with Resistance to Bacterial Pustule (Xanthomonas axonopodis pv. glycines) in Soybean (SSR 분자표지이용 콩 불마름병 저항성 관여 양적형질 유전자좌(QTL) 분석)

  • Seo, MinJung;Kang, Sung-Taeg;Moon, Jung-Kyung;Lee, Seukki;Kim, Yul-Ho;Jeong, Kwang-Ho;Yun, Hong-Tae
    • Korean Journal of Breeding Science
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    • v.41 no.4
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    • pp.456-462
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    • 2009
  • Bacterial pustule (BP), caused by Xanthomonas axonopodis pv. glycines, is prevalent disease in major soybean production areas. BP can reduce seed yield as well as seed quality. To identify the genomic region associated with the resistance to BP, QTL analysis was conducted using $F_{10}$ RIL (recombinant inbred lines) population, Keunolkong${\times}$Shinpaldalkong. Four QTLs for BP disease were identified on the linkage group B2, D2, I and K in field accounts for 36.4% of the phenotypic variation. Especially, QTL at near of Satt135 on LG D2 was identified in green house experiment explaining 20.9% of the phenotypic variation was found to be a major QTL conferring BP. One of these QTLs, Satt135 on the LG D2, was also identified in green house experiment. In both field and green house condition, the position of major QTL for BP was detected between Satt135 and Satt397 on the LG D2. The major QTL for BP may be used for minimizing soybean BP through effective marker-assisted selection (MAS).

Development of A Turn Label Based Optimal Path Search Algorithm (Turn Label 기반 최적경로탐색 알고리즘 개발)

  • Meeyoung Lee
    • The Journal of The Korea Institute of Intelligent Transport Systems
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    • v.23 no.2
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    • pp.1-14
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    • 2024
  • The most optimal route-search algorithm thus far has introduced a method of applying node labels and link labels. Node labels consider two nodes simultaneously in the optimal route-search process, while link labels consider two links simultaneously. This study proposes a turn-label-based optimal route-search technique that considers two turns simultaneously in the process. Turn-label-based optimal route search guarantees the optimal solution of dynamic programming based on Bellman's principle as it considers a two-turn search process. Turn-label-based optimal route search can accommodate the advantages of applying link labels because the concept of approaching the limit of link labels is applied equally. Therefore, it is possible to reflect rational cyclic traffic where nodes allow multiple visits without expanding the network, while links do not allow visits. In particular, it reflects the additional cost structure that appears in two consecutive turns, making it possible to express the structure of the travel-cost function more flexibly. A case study was conducted on the metropolitan urban railway network consisting of transportation card terminal readers, aiming to examine the scalability of the research by introducing parameters that reflect psychological resistance in travel with continuous pedestrian transfers into turn label optimal path search. Simulation results showed that it is possible to avoid conservative transfers even if the travel time and distance increase as the psychological resistance value for continuous turns increases, confirming the need to reflect the cost structure of turn labels. Nevertheless, further research is needed to secure diversity in the travel-cost functions of road and public-transportation networks.

A Search for Sources of Resistance to Powdery Mildew (Leveillula taurica (Lév.) Arn) in Pepper (Capsicum spp.) (고추 흰가루병에 대한 저항성 재료 탐색)

  • Lee, Ok-Hee;Hwang, Hee-Sook;Kim, Joo-Young;Han, Jeung-Hye;Yoo, Young-Shin;Kim, Byung-Soo
    • Horticultural Science & Technology
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    • v.19 no.1
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    • pp.7-11
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    • 2001
  • A total of 238 accessions of peppers (Capsicum spp.) were evaluated for resistance to powdery mildew (Leveillula taurica (Lev.) Arn) in 1998-1999. KC604, 605, 606 and 608 of C. baccatum, KC616 of C. chinense, and KC638, 640, 641, 642, 643 and 644 of C. pubescens were highly resistant and remained disease-free. KC47-1 (PI244670), KC319-1, KC545, KC320 showed only mild infection compared with susceptible control group, 'Chungok' and others. Therefore, they were considered moderately resistant. Among them, KC47-1, KC319-1 and KC320 are sources of resistance to gray leaf spot caused by Stemphylium spp. while KC47-1 is also resistant to bacterial leaf spot (Xanthomonas campestris pv. vesicatoria (Doidge) Dye). Therefore, they may be used in breeding for multiple resistance to those diseases.

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Evaluation of Weathering Durability of Waterborne Preservative Treated Wood by Accelerated Weathering (수용성 방부처리재의 기상열화 저항성 평가)

  • Lee, Myung-Jae;Lee, Dong-Heub;Kim, Gyu-Hyeok
    • Journal of the Korean Wood Science and Technology
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    • v.31 no.4
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    • pp.44-49
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    • 2003
  • This study was carried out to evaluate the weathering durability of waterborne preservative (AAC, ACQ, CCA, CuAz) treated Japanese red pine (Pinus densiflora S. et Z.) sapwood samples by accelerated weathering, and to find out the factor of stability. When considered the color changes, weight losses, surface degradation, and microstructure changes due to weathering, ACQ-, CCA-, and CuAz-treated samples were durable against weathering; the weathering durability of AAC-treated samples was poor and similar to untreated controls. The lignin content in aqueous extracts collected from ACQ-, CCA-, and CuAz-treated samples during weathering was lower than that from untreated and AAC-treated ones. From these findings, we might concluded that weathering durability of ACQ-, CCA-, and CuAz-treated samples was enhanced by the fixation of preservative component(s) onto the lignin structure, which is very susceptible to weathering.

Biorational Screening System Using Cyanobacteria(Anacystis nidulans $R_2$) for Searching the Photosynthetic Electron Transport Inhibitors (Cyanobacteria를 이용한 광합성 전자전달저해제의 생합리적 스크리닝)

  • Hwang, I.T.;Hong, K.S.;Cho, K.Y.;Yoshida, S.
    • Korean Journal of Weed Science
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    • v.13 no.2
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    • pp.81-88
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    • 1993
  • For searching the photosynthetic electron transport(PET) inhibitors, bio-rational screening system using thylakoid membranes extracted from wild and mutant cyanobacteria(Anacystis nidulans $R_2$) was developed. Generally, thylakoid membrane was more sensitive to the tested herbicides than the chloroplast from spinach in the Hill reaction. Higher resistant characteristics appeared in mutant D-5, Di-22 to diuron and mutant G-264 to atrazine as compared to wild type. To test the reaction of thylakoid membrane to herbicides, diuron and atrazine were applied simultaneously. Diuron and atrazine competed each other for binding with substituted amino acids, while diuron and dinoseb were non-competitive, and inhibiting activity was increased. Conclusively, bio-rational screening system using cyanobacteria was proved to be fast and efficient screening method for the development of PET inhibitors.

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기업(企業)의 구조조정(構造調整)과 마케팅 성과(成果)에 관(關)한 연구(硏究) -Downscaling을 중심(中心)으로-

  • Kim, Gyeong-Hun;Park, Yeong-Geun;Gang, Jing-Sik
    • Journal of Global Scholars of Marketing Science
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    • v.4
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    • pp.25-51
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    • 1999
  • 본 연구의 목적은 우리나라가 경제불황으로 인하여 국내 산업전반에 걸쳐 구조조정을 실시하게 되었음에도 불구하고, 구조조정을 효율적으로 수행하기 위한 구조조정 관리모형이나 구조조정 영향변수 등 선행연구가 전무한 상태였다. 그래서 우리나라 현실에 맞는 구조조정 관리모형을 도출하는데 있다. 이러한 목적올 달성하기 위해 구조조정관련 영향변수와 그리고 이들 변수와 마케팅 성과간의 관계를 검증하고자 한다. 이론적 배경으로는 구조조정 전략 영향변수로 구조조정전 성과, 사전구조조정 경험, 외부압력, 그리고 변화에 대한 저항 정도를 탐색하고, 그리고 구조조정 전략실행강도에 영향을 주는 인적자원의존도, 관리적 강도, 그리고 마케팅 성과에 대해 탐색했다. 실증분석을 통해 얻은 구조조정 관리모형은 인력감소, 사전구조조정 경험, 변화에 대한 저항정도가 구조조정전략 실행강도에 영향을 미치며, 이는 다시 관리적 강도에 영향을 미치는 것으로 나타났다. 그리고 관리적 강도는 마케팅 성과에 영향을 주는 요인으로 발견되었다.

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Mapping of RFLP Markers Linked to Bacterial Blight Resistant Genes (Xa-1, Xa-3) in Rice (벼 흰잎마름병 저항성 유전자(Xa-1, Xa-3)연관 RFLP 마커 탐색)

  • 강현중;김현순;남정권;이영태;이승엽;김석동
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.48 no.6
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    • pp.419-423
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    • 2003
  • Bacterial blight caused by Xantomonas oryzae pv. oryzae is one of the most serious diseases of rice especially in southern area of Korea. Three races, $\textrm{K}_1$, $\textrm{K}_2$ and $\textrm{K}_3$, are the most dominant species. lo improve rice breeding efficiency using marker assisted selection, some RFLP markers were surveyed for polymorphism between resistant and susceptible to $\textrm{K}_1$ and $\textrm{K}_3$. And, 127 doubled-haploid (DH) lines derived from Milyang121/HRl1650-1-4-2 and 131 DH lines derived from Milyang123/HR10624-AC5 were evaluated to bacterial blight ($\textrm{K}_1$ and $\textrm{K}_3$). Milyang121 and HR10624-AC5 have Xa-1, resistant to $\textrm{K}_1$ race, and Milyang123 has Xa-3, resistant to $\textrm{K}_1$ and $\textrm{K}_3$ race. Three markers, RZ590, RZ536 and RG303, showing polymorphism between parents and resistance gene, Xa-1 and Xa-3, were analysed in the two combinations of DH lines. The segregation pattern of resistant DH population of Milyang123/HR10624-AC5 to susceptible showed 3:1 and 1:1 in $\textrm{K}_1$ and $\textrm{K}_3$ race. In three RFLP markers, RZ590 was linked to Xa-1 on chromosome 4, and RZ536 and RG303 were linked to Xa-3 on chromosome 11. The map distance between Xa-1 and RZ590 was 3.1cM on chromosome 4, and Xa-3 and RZ536/RG303 were 7.6/16.0cM on chromosome 11, respectively. The results of RFLP mapping will be useful for the selection and pyramiding of bacterial blight resistant genes.

Identification of a Locus Associated with Resistance to Phytophthora sojae in the Soybean Elite Line 'CheonAl' (콩 우수 계통 '천알'에서 발견한 역병 저항성 유전자좌)

  • Hee Jin You;Eun Ji Kang;In Jeong Kang;Ji-Min Kim;Sung-Taeg Kang;Sungwoo Lee
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.68 no.3
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    • pp.134-146
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    • 2023
  • Phytophthora root rot (PRR) is a major soybean disease caused by an oomycete, Phytophthora sojae. PRR can be severe in poorly drained fields or wet soils. The disease management primarily relies on resistance genes called Rps (resistance to P. sojae). This study aimed to identify resistance loci associated with resistance to P. sojae isolate 40468 in Daepung × CheonAl recombinant inbred line (RIL) population. CheonAl is resistant to the isolate, while Daepung is generally susceptible. We genotyped the parents and RIL population via high-throughput single nucleotide polymorphism genotyping and constructed a set of genetic maps. The presence or absence of resistance to P. sojae was evaluated via hypocotyl inoculation technique, and phenotypic distribution fit to a ratio of 1:1 (R:S) (χ2 = 0.57, p = 0.75), indicating single gene mediated inheritance. Single-marker association and the linkage analysis identified a highly significant genomic region of 55.9~56.4 megabase pairs on chromosome 18 that explained ~98% of phenotypic variance. Many previous studies have reported several Rps genes in this region, and also it contains nine genes that are annotated to code leucine-rich repeat or serine/threonine kinase within the approximate 500 kilobase pairs interval based on the reference genome database. CheonAl is the first domestic soybean genotype characterized for resistance against P. sojae isolate 40468. Therefore, CheonAl could be a valuable genetic source for breeding resistance to P. sojae.