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Studies on the Hydrolysis of Holocellulose with Trichoderma viride Cellulase. (III). Effects of the Optimum Treated Conditions and Reactivation of Residue of Digested Substrates (Cellulase에 의한 목재당화(木材糖化)에 관(関)한 연구(硏究) - (III) 최적(最適) 처리조건(処理條件)과 효소처리(酵素処理) 잔사(殘渣)의 재기질화(再基質化) 효과(效果) -)

  • Min, Du Sik
    • Journal of Korean Society of Forest Science
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    • v.49 no.1
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    • pp.1-5
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    • 1980
  • In this study, enzymatic saccharification of substrates from Alnus hirsuta Ruper (8-14 years). Quercus acutissima Carruthers, Betula platyphylla var. japonica Nera, Populus euramericana Guiner and Platanus orientalis L. were investigated using crude cellulase preparations of Trichoderma viride Pers. ex. Fr. SANK 16374, and conduced on the optimum treated conditions of the cellulase sacchrification and reactivation of residue of digested substrates. The Trichoderma viride cellulase was produced by the submerged culture process and produced in the culture fluid was salted out quantitatively by the use of ammonium sulfate. The method of dilignification from wood (5 species) was treated by the peracetic acid(PA) method. The reducing sugar was determined by the dinitrosalicylic acid (DNS) method. 1. The results of tests carried out for 96 hr. (Figure 1), show conclusively the initial substrates from 5 species ($S_3$) which has been rendered highly reactive form and the mean rate of reducing sugar was 28.3 %. 2. The results of tests carried out for 96 hr., the reactivation of residue of digested substrates (improvement in the quality of the substrate through preheating in air at $190^{\circ}C$. for 45 min. followed by milling was (60 mesh size) at the same substrate level, increased concentrations of cellulase at the same substrate level, and increased concentrations of cellulase increases the rate of hydrolysis considerably. 3. Figure 1. shows conclusively that the residue of digested substrates ($S_1$ dried at $60^{\circ}C$) which has been rendered extremly resistant to cellulase action can be reactivated into a highly reactive form ($S_2$), almost comparable to that of the initial substrates ($S_3$). And the reducing sugar formation did not show statistically significent differences at 5% levels by initial substrates and the residue of digested substrates (preheating in air at $190^{\circ}C$. for 45 min. fallowed by milling was (60 mesh size).

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Effects of IBA, and NAA on the Rooting of Wild Hydrangea serrata for. acuminata (자생 산수국(Hydrangea serrata)의 발근에 미치는 IBA와 NAA의 영향)

  • Ryu, Mi-Jin;Park, Byoung-Mo;Bae, Jong-Hyang
    • Journal of Bio-Environment Control
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    • v.19 no.4
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    • pp.397-402
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    • 2010
  • This study was conducted to find out the appropriate type and concentration of plant growth regulator for mass production of wild type Hydrangea serrata. And the optimal soaking time for rooting of the cuttings was also investigated. When the cuttings of Hydrangea serrata were cultivated for 6 weeks after immersing in $50mg{\cdot}L^{-1}$ of IBA for 3 hrs, the number of roots was 90.6. However the number was significantly decreased when the cuttings were treated with $10mg{\cdot}L^{-1}$ of IBA. The rooting ratio was 100% when the cuttings was immersed in 1,000, 2000 and $3000mg{\cdot}L^{-1}$ of NAA solution for 5, 3 and 10 sec., respectively. Despite the perfect rooting, the number of roots was decreased. The number of roots was 105.5 when the cuttings were treated in $2,000\;mg{\cdot}L^{-1}$ of NAA for 5 sec., which was the highest number in NAA treatment. Overall, the rooting ratio and growth was better when the cutting was treated with low concentration of IBA ($50{\sim}250\;mg{\cdot}L^{-1}$) than the treatment of NAA. And the most appropriate concentration and time fot the treatment of IBA was $50mg{\cdot}L^{-1}$ and 3 hrs, respectively.

Analysis to Determine the Employment Status of Married Women's on the Social Factors Associated (기혼여성의 고용지위 결정요인에 관련한 사회변인 분석)

  • Hwang, Hee-Sook;Kim, Youn-Jae;Park, Jung-Woo
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
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    • v.7 no.3
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    • pp.181-190
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    • 2012
  • After industrialization, the labor force participation rates of women, especially married women is drastically increasing. So, this study was designed to analyze the determinants of married women's employment status considered. For this, the determinants of married women's employment status were divided into individual-related, children-related, household-related and job-related variables to establish the research models. Based on this, the following results were drawn from a multinominal logistic regression analysis of the determinants of married women's employment status. First, an analysis of individual-related variable showed that married women had the employment status of labor wages with residence in the center of the city and high academic background. Second, an analysis of children-related variable showed that they had the employment status of labor wages with many their children and no their children under the age of six. Third, an analysis of household-related variable showed that they had the self-employment status of labor wages with nuclear family and few income earners of family members. Finally, an analysis of job-related variable showed that they had the employment status of labor wages when they got a job before they got married, their husband didn't get a job, and their husband worked in a professional field. As for findings stated above, as there was a difference in the determinants of married women's employment status, the ways for improvement in the married women's employment status would be suggested as follows. First, married women with young children have the low employment status, basically, requiring problem-solving ways for this because the housekeeping and child-rearing burden caused by marriage and childbirth are factors that continue to obstruct a job. For this, the flexible working hours system, which housekeeping and child-rearing can harmonize with economic activities like developed countries, needs to be activated. But the activation of such flexible working system will produce actual results under institutional protection, such as a related-protection law. Second, the Leave of Child Care System is debated as one of the most representatively systems that housekeeping can harmonize with economic activities. Now, although the System is legislated, the use is very poor.

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A Study on Effective Planning Method of the Check-off Fund Program for Egg (계란자조금의 효과적인 조성방안에 관한 연구)

  • Kim, Dong Jin;Hong, Sung Kyu;Kim, Yoon doo
    • Korean Journal of Poultry Science
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    • v.44 no.1
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    • pp.51-57
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    • 2017
  • Subsequent to the introduction of the "Laws on Consumption Promotion of Livestock Products" in 2002, a mandatory check-off system for the egg industry was introduced in June 2009. Unlike other livestock products, the check-off on eggs is financed not from the sale of the egg-the end product-but from the spent hen. Variables such as the falling price of spent hens lead to a low financing rate; The present farm allotment rate of 14%, introduced in 2015, is much higher than the 0.2% to 0.3% check-off rate in other types of business, thus placing a huge burden on farms. When check-off is financed from the egg, it is expected that 6.5 billion won will be raised using a rate of 0.3% of sale proceeds, Therefore, it is urgent for the government to establish infrastructure to enable producers and merchants to do business through Grade & Packing centers. This can be achieved by the staged construction of local Grade & Packing centers. Check-off should continue to be financed from the present spent hen until this infrastructure is established, and an institutional strategy should be provided by the government to raise the financing rate accurately and transparently using statistical treatment of supply data for spent hens as a short-term alternative.

Yielding Ability and Competitive Influence of Tillering Maize(Zea mays L.) when Mixed with Uniculm Hybrid (형태적(形態的)으로 다른 두 옥수수 (Zea mays L.)의 혼작(混作)이 청예(靑刈)및 종실수량(種實收量)에 미치는 영향(影響))

  • Choe, Bong Ho;Choi, Chang Yeol;Park, Jong Seong;Lee, Hee Bong
    • Korean Journal of Agricultural Science
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    • v.15 no.2
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    • pp.123-131
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    • 1988
  • This study was done to investigate the interplanting effects of corn, when a corn hybrid with tillers (IK derivaties) was interplanted with a corn hybrid without tillers(Jinjoo Ok). The interplanting ratios of two hybrids, IK and Jinjoo Ok, were 1 to 1, 1 to 2, 1 to 3, 2 to 1, and 3 to 1. The interp1anting effects were compared with monoculture for major agronomic characteristics. 1. The plant height was increased when interplanted regardless of the interplanting ratios of the two hybrids. The general growth of IK hybrid was a little hindered when the interplanting ratio of Jinjoo Ok hybrid increased. 2. The number of tillers produced by the IK hybrid increased until 40 days after emergence and appeared to increase as the interplanting ratio of IK hybrid increased. 3. When monocultured, the fresh weight of IK hybrid per 10a was 8,096kg and and 6,677kg at silking and dough stages, respectively and these were 77%, 53% over the fresh weight of Jinjoo Ok at the sarne stages. When IK and Jinjoo Ok hybrids were interplanted in 1 to 1 ratio, the fresh weight of IK was 20% higher than that of Jinjoo Ok monocultured. 4. The highest grain yield per 10a was obtained from the Jinjoo Ok hybrid monocultured and the yield was 434kg. Even the interplanting ratios between IK and Jinjoo Ok were 1 to 1 and 1 to 3, the grain yield of Jinjoo Ok was 16% and 13% over the IK, respectively. 5. Root and stalk lodging of IK hybrid was 29% when monocultured and seemed to increase as the interplanting ratio of Jinjoo Ok increased. The lowest lodging was found when the interplanting ratio of IK and Jinjoo Ok was 1 to 1. 6. Stem diameter of IK hybrid was also decreased as the interplanting ratio of Jinjoo Ok increased. The ear height of IK was lowered as the interplanting ratio of IK was increased. The two hybrids tested seemed to have tolerance to the black streaked dwarf virus(BSDV).

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Behaviors-Based Safety Management of Dental Professionals on Dental Amalgam and Resin (치과용 아말감과 레진에 대한 치과종사자의 안전성에 대한 관리행동 실태)

  • Yoon, Sung-Uk;Nam, In-Suk
    • Journal of dental hygiene science
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    • v.13 no.1
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    • pp.97-104
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    • 2013
  • This study investigated the safety management behavior of dental professionals to minimize exposure to harmful elements of amalgam and resin. As for the use of amalgam and resin, 85.8% of them used amalgam. 100% of used composite resin 100%. 97.8% used acryl resin, and 95.7% used tray resin. 95.2% and 36.5% of them were aware of the harm of amalgam and resin respectively. When using resin, their safety management behavior score was 1.99 on average, and the lowest score was 2.50 on average for 'ventilate the indoor air when handling resin'. Their average score of the safety management behavior was 1.83 when using amalgam. The lowest score was 2.89 on average for 'use the rubber dam for patients when handling amalgam'. As a result of the safety management behavior based on general characteristics, all characteristics were significant with regard to amalgam, and my position at work and work experience were significant with regard to resin (p<0.05). The correlation between the safety management behavior and general characteristics was analyzed when dental amalgam and resin are used. Amalgam was statistically correlated with academic background (p<0.05) and position at work (p<0.05), whereas resin was statistically significantly correlated with age (p<0.05) and position at work (p<0.05). Accordingly, education about the harm of dental materials must be continuously provided for dental professionals when they handle dental materials so that safety management behavior will be conducted correctly, and active efforts to protect the health of dental professionals from harmful chemicals must be made.

Changes of General Components and Aromatic Constituents in Codonopsis lanceolata Grown at The Native and Cultivated Area (재배장소에 따른 더덕의 일반성분과 향기성분의 조성변화)

  • 이승필
    • Korean Journal of Plant Resources
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    • v.9 no.3
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    • pp.230-238
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    • 1996
  • The experiment was carried out to find aromatic constituent composition of Codonopsis lanceolata species grown in the native(Mt. lrwol, Youngyang, Kyungpuk Province) and the cultivated(Andong, Kyungpuk Province) areas from 1994 to 1995. The results were as follows : Air temperature and soil temperature at the native area were lower to $2{\sim}3^{\circ}C$, and to $2^{\circ}C$ than the cultivated area. Organic matter contents of plant grown at the native area higher than that of plants at the cultivated area by 4.8%. Crude protein content was higher in plant grown at native area than the plant grown in wild area but in case of crude saponin, plants grown at wild area was higher than that of native area. For inorganic element contents. K content is much higher than in the domesticated area as compared with wild area above ten over. And other elements such as Cu, Mn, Na and Mg were not remarkably different in contents. In the free amino acid compositions, argine was highest compared with other free amino acids, and Arginine content was higher in dometicated area. Recovery yield of essential oil of wild species grown at the cultivated showed 0.005%, but domesticated species was 0.004%. But both species at the native area were the same by 0.004%. Although composition of aromatic constituents in the two areas and species varied, total aromatic constituent was 21 kinds. Most aromatic constituents were aliphatic alcohols such as 1-hexanol, eis-3-hexanol, and trans-2-hexanol occupied by approximately 90% over. But three constituets as amylalcohol, furfuryl acetate, and 2-methoxy-4-vinyl phenol(MVP) were detected only in domesticated species.

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Influence of the Composition and Concentration of Fertilizer Solution on Growth and Nutrient Uptake of 'Maehyang' Strawberry during Vegetative Growth (비료의 조성 및 농도가 영양생장 중인 '매향' 딸기의 생장, 양분흡수 및 근권환경 변화에 미치는 영향)

  • Jang, Hyun Ho;Lee, Hee Su;Lee, Chiwon W.;Choi, Jong Myung
    • Horticultural Science & Technology
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    • v.32 no.4
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    • pp.484-492
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    • 2014
  • We quantified the effects of the composition and concentration of fertilizer solutions on the growth and nutrient uptake of 'Maehyang' strawberry at the vegetative growth stage. Acid fertilizer (AF), neutral fertilizer (NF), and basic fertilizer (BF) with concentrations of 100 or $200mg{\cdot}L^{-1}$ based on N, were formulated and applied during the 100 days after transplanting, at which point crop growth and tissue nutrient contents were analyzed. The soil solutions were sampled every two weeks to measure the pH, EC and nutrient concentrations. BF was a more effective fertilizer compared to AF and NF for growth of 'Maehyang' strawberry when fertilizer concentrations were controlled to $100mg{\cdot}L^{-1}$. By contrast, the fresh and dry weights were greatest in the NF treatment when fertilizer concentrations were controlled to $200mg{\cdot}L^{-1}$. The tissue contents of N, P, and Na were 2.20, 0.51 and 0.10%, respectively, in the NF 200 treatment, and these were the highest among all treatments tested. The highest contents of K, Ca and Mg were 2.60% in AF 200, 0.95% in BF 200 and 0.45% in BF 100, respectively. During the fertilization period, the highest and lowest pHs were 6.13 in BF 100 and 4.92 in AF 200. The lowest EC was $1.376dS{\cdot}m^{-1}$ in BF 100 and the highest was $4.936dS{\cdot}m^{-1}$ in NF 200 treatments. The highest $NH_4{^+}$ concentrations in the soil solution occurred during the AF 200 treatment, followed by those with NF 200 and AF 100. When fertilizer concentrations were equal, AF treatment gave rise to the highest $Ca^{+2}$ and $Mg^{+2}$ concentrations, followed by NF and BF, until day 84 of fertilization. The BF treatments had the highest $NO_3{^-}$ concentrations followed by NF and AF. The trends for $PO_4^{-3}$ concentrations were similar in all fertilizer treatments when the fertilizer concentrations were equal. The above results indicate that neutral and basic fertilizers were most effective to promote 'Maehyang' strawberry growth when fertilizer solutions were controlled to low or high concentrations, respectively. These findings are useful for planning fertilization programs for vegetative propagation of 'Maehyang' strawberry.

The Effect of IAA on $Colocasia$ $esculenta$'s Growth and Morphogenesis (IAA가 토란의 생장 및 형태 발생에 미치는 영향)

  • Kim, Young-Eun;Lee, Joon-Sang
    • Korean Journal of Environmental Biology
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    • v.29 no.2
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    • pp.92-97
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    • 2011
  • Morphological and physiological differences of $Colocasia$ $esculenta$ were investigated in the cultivation of hydorphonic and soil culture. $C.$ $esculenta$ grown in Hoa.+IAA (indole-acetic acid) showed higher growth activity representing 9%, 32%, 38% and 60% than those of the cultivation of vermiculate, Hoagland solution, soil and water, respectively. In case of $F_v/F_m$ ratio experiments, the value $F_v/F_m$ of $C.$ $esculenta$ cultivated in the water showed 0.55 after 6 weeks. $F_v/F_m$ values of $C.$ $esculenta$ cultivated in Hoagland+IAA, vermiculate and soil were between 0.84 and 0.80 indicating $F_v/F_m$ values were about 45% higher than that of $C.$ $esculenta$ cultivated in the water. Diffusion resistance was 45~35% lower in $C.$ $esculenta$ grown in Hoa.+IAA solution than that of $C.$ $esculenta$ grown in water only after 5 and 6 weeks. Therefore, the high standing levels of the growth rate, fluorescence activity and transpiration rate were Hoa.+IAA, vermiculate, Hoagland, soil and water. The distinct morphological differences of $C.$ $esculenta$ cultivated in hydorphonic and soil culture were the appearance of the seed corm and root hair. The development of seed corm was well established in soil culture but the corm in hydorphonic was slowly hydrolyzed and then disappeared. The fibrous root systems of hydorphonic were very well distinguishable compared with that in soil culture. Outstanding results of this experiment were appeared in $C.$ $esculenta$ which was cultivated in the field provided with enough mineral nutrition, organic fertilizers and compound fertilizers. The most height taros were almost 2m and the numbers of seed corm were 30~40 after 7 months.

Effects of Dietary Supplementation of Mixed Probiotics on Production Performance and Intestinal Environment in Broiler Chicken (혼합 생균제의 사료 내 첨가 급여가 육계의 생산성과 장내 환경에 미치는 영향)

  • Oh, Seong Taek;Kang, Chang Won;Kim, Eun Jib
    • Korean Journal of Poultry Science
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    • v.41 no.2
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    • pp.143-149
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    • 2014
  • This study was conducted to investigate the effects of dietary supplementation of the mixture of probiotics (MP) on growth performance, size of small intestine, cecal microflora and ammonia concentrations in broiler chicks. A total of 700, one-day-old male broiler chicks were randomly allotted to four treatments with seven replications having 25 birds per pen. The birds were fed one of the four experimental diets; containing no antibiotics nor MP (negative control, NC), containing antibiotics without MP(positive control, PC), negative control with MP 0.1% and negative control with MP 0.2% for 5 weeks. During overall experiment, birds in PC and MP treatments had higher final BW and daily BW gains than birds in NC treatments; however, the significance was not identified. The feed conversion ratio of the chicks fed the diet containing MP was significantly improved as compared to those fed the NC diets. The weights of jejunum were increased by the MP (p<0.05), but weights of duodenum, ileum and length of small intestine were similar among the groups. Birds in PC treatment showed lower populations of total microbes and lactic acid bacteria than other groups (p<0.05), cecal ammonia concentrations of the chicks fed the diet containing MP were significantly decreased as compared to those of NC and PC (p<0.01). In conclusion, MP added to the broiler diets improved the feed conversion rate and reduced cecal ammonia concentration.