• Journal of Korean Medical classics
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• v.13 no.1
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• pp.146-183
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• 2000

통과이상적연구(通過以上的硏究), 장상한학술적성숙기(將傷寒學術的成熟期), 칙지명대여청대관여상한의학사적내용정리(則至明代與淸代關與傷寒醫學史的內容整理) 여하(如下). 1. 명청시대적시대배경사상한학적내용갱풍부(明淸時代的時代背景使傷寒學的內容更豊富), 촉진상한학술적발전(促進傷寒學術的發展) 병차출현료흔다관여상한적저작(幷且出現了?多關與傷寒的著作), 단실제상야출현료상한론내용적중복여지론술일반부분(但實際上也出現了傷寒論內容的重複與只論述一般部分), 혹저자립안조잡적처방지폐단(或著者立案粗雜的處方之弊端). 차기우출현각종상한학파적리유(此期又出現各種傷寒學派的理由), 시인위당시류행적사조(是因爲當時流行的思潮), 칙정주리학적영향여명대문단부고(則程朱理學的影響與明代文壇復古), 의고적추향(擬古的趨向), 환유금원시대적백가쟁오등(還有金元時代的百家爭嗚等), 성료형성상한학파적기초(成了形成傷寒學派的基礎). 2. 위료불실거상한론적본래의식(爲了不失去傷寒論的本來意識), 주장정리화고정원문회부왕숙화이전모양적착간중정파시왕안도기료개단(主張整理和考訂原文恢復王叔和以前模樣的錯簡重訂派是王安道起了開端), 방유집확대료기의식(方有執擴大了其意識). 유창여침명종시대표저학파학자(喩昌與沈明宗是代表這學派學者), 장로(張?) 정응모(程應?) 주양준(周揚俊) 오겸등수료저학설(吳謙等隨了這學說). 3. 주장(主張)'존왕(尊王)(숙화(叔和))찬성(贊成)(무기(无己))'적유호구론파(的維護舊論派), 주장료불능수변개상한론삼음삼양편적배렬여순서(主張了不能隨變改傷寒論三陰三陽篇的排列與順序), 문자(文字), 구문(句文), 문장(文章), 고세식(高世?), 진념조등인(陳念祖等人). 4. 주장상한론적정수재어변증론치(主張傷寒論的精髓在於辨證論治), 선우운용칙능득상한핵심리론적학파시변증론치학파(善于運用則能得傷寒核心理論的學派是辨證論治學派). 저학파분사개계통(這學派分四?系統), 이처방수집증상적의가유허굉(以處方收集證狀的醫家有許宏), 가금(柯琴), 서대춘(徐大椿); 이치법수집증상적의가유오인구(以治法收集證狀的醫家有吳人駒), 우이(尤怡); 이분유륙경찰증상적의가유진념조(以分有六經察證狀的醫家有陳念祖), 포성(包誠); 이증상분류증후적의가유류순(以證狀分類證候的醫家有劉純), 왕긍당(王肯堂), 진지정(秦之楨), 침금오등인(沈金鰲等人). 5. 회통파유량종류형(?通派有兩種類型), 일시수통상한여온병적학파(一是誰通傷寒與溫病的學派), 간칭회통파(簡稱?通派), 대표의가유도화(代表醫家有陶華), 오정(吳貞), 유근초등인(兪根初等人), 령일개유회통중의여서의적립장상해석상한적의가당종해(?一?有?通中醫與西醫的立場上解釋傷寒的醫家唐宗海). 6. 경전학파시이상한론위보귀적경전래인식(經典學派是以傷寒論爲保貴的經典來認識), 병차지유숭상차경여사상적학파(幷且持有崇尙此經與思想的學派), 차리분량개편우원문적고증래연구적경전고증파(此里分兩?偏于原文的考證來硏究的經典考證派), 화인위지유상한론(和認爲只有傷寒論), 재능총괄치료외감병(才能總括治療外感病), 부정온병학설병배척적경전림상파(否定溫病學說幷排斥的經典臨床派).

• Tuberculosis and Respiratory Diseases
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• v.63 no.2
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• pp.165-172
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• 2007

Background: The pathogenesis of lung cancer includes the accumulation of multiple genetic abnormalities. The CREB-binding protein(CBP) is one of several transcriptional co-activators among various sequence-specific DNA-binding transcription factors. CBP is involved in a wide range of cellular activities, such as DNA repair, cell growth, differentiation, and apoptosis that are suspected of contributing to tumorigenesis. The goal of this study was to evaluate CBP expression in a series of human lung tissues containing normal epithelium, premalignant lesions(hyperplasia and dysplasia) and squamous cell carcinomas. Materials and Methods: Immunohistochemical staining was performed on formalin-fixed paraffin-embedded sections by use of a monoclonal anti-CBP antibody. CBP expression was compared in samples from 120 patients with premalignant and malignant histological types including 20 metaplastic specimens, 40 dysplastic specimens, and 60 squamous cell carcinomas in the lung. Results: CBP expression was seen in 35% (7/20) of the metaplastic specimens. 65% (26/40) of the dysplastic specimens, and 70% (42/60) of the squamous cell carcinomas (p<0.05). According to celluar atypism, CBP expression was 50% (10/20) of the low-grade dysplastic specimens and 80% (16/20) of the high-grade dysplastic specimens(p <0.01). By cellular differentiation, CBP expression was seen in 95% (19/20) of the well differentiated squamous cell carcinomas, 85% (17/20) of the moderately differentiated carcinomas and 30% (6/20) of the poorly differentiated lesions (p <0.05). Conclusion: These results suggest that CBP may have an important role in malignant transformation of precancerous lung lesions and may be a marker for malignancy.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.30 no.3 s.47
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• pp.389-392
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• 2004

We screened several materials to stimulate IGF-1 promoter activity using luciferase reporter assay and found that Amomi Semen extract (ASE) among them is the most powerful stimulator We also studied about the anti-wrinkle effect of ethanolic extract of Amoni Semen in vitro and in vivo. Semi-quantitative RT-PCR showed that the extract elevated the presence level of IGF-1 mRNA. And $[^3H]$ proline incorporation and semi-quantitative RT-PCR showed that the extract increased the expression of type-I collagen compared with vehicle in vitro and in vivo, respectively. Significant inhibition of MMP-1 expression was determined by ELISA and Western blot. Finally, topical treatment of the extract on hairless mouse's dorsal skin expanded the volume of collagen and dermal thickness. These results suggest that Amomi Semen may be a good candidate for improving extracellular matrix through the increase of collagen expression and inhibition of MMP-1 expression. Moreover, this study enables us to guess that IGF-1 stimulated by the extract may be involved in the mechanism of anti-wrinkle effect of it.

• Journal of Korean Society of Forest Science
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• v.99 no.4
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• pp.534-545
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• 2010

To examine 1) rainfall pattern (i.e., type and intensity) regulating surface erosion on hillslopes in postwildfire area and 2) its effect on variation in sediment yield along the gradient of severity wildfire regimes and elapsed years, we surveyed the amount of sediment yield with respect to daily or net-effective rainfall in 9 plots in eastern coastal region, Republic of Korea. Before field investigation, all plots classified into three groups: low-, mixed- and high-severity wildfire regimes (3 plots in each group). We found that, with decreasing wildfire regimes and increasing elapsed years, the rainfall type regulating surface erosion changed from daily rainfall to net-effective rainfall (considering rainfall continuity) and its intensity increased continuously. In general, wildfires can destroy the stabilized forest floors, and thus rainfall interception by vegetation and litter layer should be reduced. Wildfires can also decrease soil pores in forest floors, and thus infiltration rates of soil are reduced. These two processes lead to frequent occurrence of overland flows required to surface erosion, and sediment yields in post-wildfire areas should increase linearly with increasing rainfall events. With the decreasing severity wildfire regimes and the increasing elapsed years, these processes should be stabilized, and therefore their sediment yields also decreased. Our findings on variations in sediment yields caused by the wildfire regimes and the elapsed years suggest understanding of hydrogeomorphic and ecologic diversities in post-wildfire areas, and these should be carefully examined for both watershed management and disaster prevention.

• Journal of Life Science
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• v.15 no.4 s.71
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• pp.626-632
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• 2005

The polyamines are essential components of all eukaryotic cells and absolutely necessary for cell growth. In the present study, the cytoprotective role of polyamine was characterized. When $Ac_2F$ rat liver cells were treated with 1M 2,2'-azobis (2-amidinopropane) dehydrochloride (AAPH), a water soluble free radical initiator, viability of the cells was noticeably decreased due to the increase of reactive oxygen species (ROS). The cytotoxic effect of AAPH as well as ROS generation were significantly inhibited by the treatment of polyamines. Among polyamines, especially spermine at $20{\mu}M$ concentration exerted over $45\%$ inhibition of AAPH-induced ROS generation. Western blotting was performed to determine whether superoxide dismutase(SOD) or catalase (CAT) expression was involved in oxidative stress. The AAPH treatment blocked both SOD and CAT protein expressions. Spermine could recover those protein expressions to the untreated control levels. According to the result of cycline E measurement, AAPH might block the entry of the cells into S phase of the cell cycle. The reduced expression of cyclin E protein could be fully recovered by the addition of spermine. The antioxidative effects of spermine was also further proved by the apopotitic morphological analysis using ethidium bromide and acridine orange.

• Korean Journal of Food Science and Technology
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• v.50 no.3
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• pp.349-355
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• 2018

Ginsenoside Rg5, one of the protopanaxadiol ginsenosides of wood-cultivated ginseng, has been implicated in various diseases, such as diabetes, cancer, and hypertension; however, its angiogenic activity and molecular mechanisms have not yet been elucidated. Here, we found that wood-cultivated ginseng extract and ginsenoside Rg5 increase in vitro proliferation, migration, and tube-like structure formation, which are typical phenomena associated with angiogenesis, in cultured human umbilical vein endothelial cells (HUVECs). Moreover, Ginsenoside Rg5 stimulated the phosphorylation of Akt, endothelial nitric oxide (NO) synthase (eNOS), and extracellular-regulated kinase (ERK)1/2, which are well-known signal mediators of the angiogenic pathway. Furthermore, Ginsenoside Rg5 did not accelerate the activation of ICAM-1 and VCAM-1 which are inflammatory response mediators. These results suggest that wood-cultivated ginseng extract and ginsenoside Rg5 stimulated in vitro angiogenesis by activating the Akt/eNOS- and ERK1/2-dependent signal pathways without inducing vascular inflammation.

• Journal of Nutrition and Health
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• v.47 no.2
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• pp.106-112
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• 2014

Purpose: This study was conducted in order to investigate the protective effects of ethanolic extract of Acanthopanax koreanum Nakai (AE) against carbon tetrachloride ($CCl_4$)-induced liver injury in rats. Methods: Male Sprague-Dawley rats were randomly divided into four groups in order to receive the following experimental diets with intraperitoneal injection of $CCl_4$ (2.0 mL/kg body weight, 20% solution 0.65 mL) for eight weeks (n = 8 per group): $CCl_4$ control (CON), $CCl_4$ + AE 1% (AE1), $CCl_4$ + AE 3% (AE3), or $CCl_4$ + acanthoic acid 0.037%, which is equivalent to AE 3% (AA). Results: Highest serum ALT activity and albumin level were observed in the $CCL_4$ control group, but showed a significant decrease by either AE or AA supplementation in a dose-dependent manner (p = 0.0063 and 0.0076, respectively). Both hemotoxylin and eosin staining and Masson's staining indicated remarkable prevention of $CCl_4$-induced liver damage in the AE3 group. $TNF{\alpha}$ and IL-6 production were significantly lowered in the AE treated groups, but not in the AA group (p = 0.0016 and p = 0.0002, respectively). The effects of AE3 were greater than those of AA for inflammation and liver toxicity biomarkers. Conclusion: Taken together, the results suggested that ethanolic extract of Acanthopanax koreanum Nakai provided hepatoprotective effects, leading to the reduction of inflammatory response. In addition, the effect of AE was superior to that of single compound AA.

• Korea Science and Art Forum
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• v.37 no.2
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• pp.205-217
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• 2019

This study began with the focusing the growth of importance of cultural marketing and its successes by applying to branding in a phase in which the cultural production era has arrived after the industrial production era. This study aimed to reveal the effect of cultural marketing, which are used as marketing strategy in the tourism field, on brand attitude and awareness. Therefore, this study tried to investigate the brand equity and attitude in accordance with the cultural marketing factors by survey Chinese tourists visiting Korea. The result and contents of the study are as follows. First, it was confirmed that the research question on the possibility of market segmentation in accordance with cultural marketing factors; the market segments were classified as Cluster 1 general involvement perceived group, Cluster 2 high involvement perceived group, Cluster3 middle involvement perceived group. Second, it revealed significant differences on brand attitude among the segments; Cluster 2 showed high brand attitude and Cluster 3 showed average level on brand attitude. Third, it found a statistically significant differences in the perception of brand equity including perceived quality, brand awareness and brand image of tourism destination. In conclusion, this study was able to identify market segments in accordance with cultural marketing factors of tourism destination and revealed significant differences in the segments by brand attitude and brand equity. This could provide practical implication that various cultural marketing activities to generate positive results on brand equity and brand attitude and cultural marketing strategies is also needed to increase foreign tourists' awareness of these cultural marketing activities.

• Food Science and Industry
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• v.51 no.4
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• pp.302-312
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• 2018

Bioactive peptides generated from milk proteins play an important role in the prevention and alleviation of diabetes. Whey proteins possess direct insulinotropic effect by amino acids (especially branch chain amino acids) produced through its gastrointestinal digestion. Additionally, blood glucose level can be lowered by gut hormone which called incretin [glucose dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1)]. However, physiological effects of incretin readily disappeared by dipeptidyl peptidase-4 (DPP-4) causing degradation of GLP-1. Several DPP-4 inhibitors are currently used as therapeutic medicines for the treatment of type II diabetes. More than 60 natural peptide (2-14 amino acids) DPP-4 inhibitors were identified in milk proteins. Peptide DPP-4 inhibitors act as substrate inhibitor and delay breakdown of GLP-1 both in vitro and in vivo. This review summarizes nutritional quality of milk proteins, absorption and mode of action of bioactive peptides, and finally up-to-dated knowledge on DPP-4 inhibitory peptides derived from milk proteins.

• Journal of the Korean Society of Radiology
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• v.13 no.1
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• pp.125-132
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• 2019

Potassium cyanate (KCN) is an inorganic reagent and can induce the post-translation carbamylation of proteins. The carbamylated reaction in the body is involved in cell death in various diseases. According the results in our previous study, KCN enhances the radiosensitivity of human colorectal cancer cell line, HCT 116 cells. However, it was not enough to confirm the mechanism that KCN works in these cells. To determinated the mechanisms of KCN in the cells with increased radiosensitivity, HCT 116 cells were treated KCN with low-dose gamma-radiation. And then, we examined alteration of the cell cycle, cell proliferation, cytokine level and the activation of cell signaling protein. As a result, cell cycle arrest and cell death were induced by the activation of caspase-3 and PARP in the irradiated cells with KCN treatment. These changes of the irradiated cell with KCN treatment were induced by the release of $TNF-{\alpha}$ via $NF-{\kappa}B$ activation. In conclusions, enhanced radio-sensitivity mediated by KCN induced cell death and it occurs by $NF-{\kappa}B$-dependent $TNF-{\alpha}$ production.