• Title/Summary/Keyword: 장화(張華)

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Characteristics of Antifungal Bacterium, Bacillus subtilis YS1 and It′s Mutant Induced by Gamma Radiation (온천수로부터 분리한 항진균세균의 특성 및 감마선$(Co^{60})$ 조사를 이용한 돌연변이체 유기)

  • 이영근;김재성;송인근;정혜영;장화형
    • Korean Journal of Microbiology
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    • v.37 no.4
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    • pp.305-311
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    • 2001
  • Antifungal bacterium, Bacillus subtilis YS1 was isolated from Yusong hot spring showed broad antifungal spectrum against 12 kinds of plant pathogenic fungi and Candida albicans, animal pathogen. From the gamma($Co^{60}$) radiation sensitivity test, $D_10$ value was 2.08 kGy and it survived above 20 kGy of radiation dose. Several mutants were induced by gamma radiation. Among them, YS1-1009 mutant showed resistance against tebuconazole of herbicide, increased activity against Botryoshaeria dothidea and ligninase activity. YS67 mutant was antifungal deficient auxotrophic mutants(trp-pro-or arg-ura-). From this results, it suggested that gamma irradiation could be useful method for mutant induction.

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BIOLOGICAL ACTIVITIES OF PRODUCTS FROM SUGAR CANE BAGASSE FERMENTATION BY Pleurotus sajor-caju (사탕수수 찌꺼기를 이용한 여름느타리 발효부산물의 생물활성)

  • Lee, Young-Keun;Chang, Hwa-Hyoung;Kim, Won-Rok;Kim, Jin-Kyu;Kim, Jae-Sung
    • Korean Journal of Environmental Agriculture
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    • v.17 no.1
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    • pp.39-42
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    • 1998
  • In order to evaluate the biological activities of some fractions from the fungal(Pleurotus sajorcaju) fermentation products of sugar cane bagasses, the antimutagenicity, the glyceollin elicitor activity on soybean and the effect on the stem elongation in pea were observed. The alcohol extract fraction and DMSO soluble fraction had excellent antimutagenicity even though it is weaker than that of the extracts from the fruiting bodies. All of the extracts had the ability to elicit glyceollins in soybean cotyledons and these extracts could be helpful for plants to protect themselves from pathogenic contaminations. IAA and the extracts had shown synergistic effects on pea stem elongation in all experimental groups positively determined.

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A Study on the Change of Strength of FRP Member Immersed in Chemical Solution (화학약품용액에 침지한 FRP 부재의 강도 변화에 대한 연구)

  • Kim, Ho-Sun;Kim, Woo-Jong;Jang, Hwa-Sup;Kwak, Kae-Hwan
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.14 no.6
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    • pp.117-123
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    • 2010
  • If FRP materials that have been known as high durability materials are exposed to harmful environmental factors, deterioration and characteristics of materials can be reduced due to chemical reaction such as hydrolysis. Therefore, to use FRP materials as building major materials, it is important to exactly grasp dynamic properties by use condition. Accordingly, this study stored FRP materials in a strong acid and alkali compound solution for a certain period to conduct simulation for acute or chronic, extreme changes by chemicals, and conducted a test for compressive, tensile, shear and bending strength to analyze changes in strength by kinds and storage days of chemicals. In conclusion, the study findings indicate excellent chemical resistance of FRP materials.

Characteristics of the Child Maltreatment at Day Care Centers and the Case Dispositions of Child Protective Services in Korea (한국 어린이집 아동학대의 학대 특성 및 아동보호서비스의 최종조치 관련 요인 분석)

  • Kim, Kihyun;Jang, Hwajung;Kim, Kyunghee;Jang, Heesun
    • Korean Journal of Social Welfare
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    • v.66 no.4
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    • pp.25-52
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    • 2014
  • This study examined the characteristics of child maltreatment cases which occurred recently at the Day Care Centers in Korea. The study utilized the case records system of the National Child Protection Agency (K-CPS) and analyzed all the cases reported to the K-CPS from 2010 to 2012 (n=462). Using the case records abstraction system developed for this study, based on the Maltreatment Case Records Abstraction System (MCRAI; Trickett et al., 2009), the detailed information on abuse cases as well as disposition outcomes was abstracted. The results showed the relatively low rate of abuse designation, low rate of reports from the mandatory reporters as well as overwhelmingly high rate of physical abuse. Diversity was detected in severity ratings in each type of abuse. Finally, determinants of dispositions differed for children, perpetrators and daycare centers. Based on the results, implications for practice and child maltreatment research were discussed.

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Effects of Film Thickness and Moisture Absorbing material on Ginger Quality during MA Storage (필름두께 및 흡습제 처리에 따른 생강의 MA 저장효과)

  • 정문철;남궁배;김동만
    • Food Science and Preservation
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    • v.6 no.3
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    • pp.264-269
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    • 1999
  • The effects of film thickness and moisture absorbing materials(MAM) on the ginger qualities were investigated during MA storage for 150 days. LDPE films of 0.04, 0.06 and 0.08mm thicknesses were applied to select the adequate film in gas permiability. MAMs of sheet and sachet type were applied to 0.06mm-LDPE film bag to prevent moisture condensation during storage. Thickness of film used affected neither weight loss nor firmness of gingers during U storage. But the thinner in thickness showed the less changes in sprouting, spoilage and reducing sugar. Moreover, compared with MAP alone, MAPs with MAM of both sachet and sheet styles led to the weight loss of gingers increasing by more than 3-4 times, but sprouting rate, spoilage rate and reducing sugar decreasing by 3.3, 1.4 and 2.3 times, respectively. These results showed that MAP with UM was significantly effective to prolong the freshness of ginger by longer than 2 times, compared with MAP alone.

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Survey of Corneal Thickness, Endothelial Cell and Intraocular Pressure in New Zealand White Rabbits (뉴질랜드 흰 토끼의 각막 두께, 내피세포 및 안압에 관한 조사)

  • Chang, Hwa-Seok;Lee, Dae-Yeoup;Kang, Eun-Hee;Lee, Jae-Hoon;Cho, Yang-Kyeung;Kim, Man-Soo;Kim, Hwi-Yool
    • Journal of Veterinary Clinics
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    • v.27 no.4
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    • pp.411-414
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    • 2010
  • Rabbit's cornea is the most common thing that used for researches in ophthalmology, so many experimentations have done. The purpose of this study was to survey the corneal pachymetry, tonometry and specular microscopy in New Zealand white rabbits. Three months old male rabbits ($2.9{\pm}0.2\;kg$) were examined with contact the specular microscope to evaluate the endothelial cell density, coefficiency of variation and the percentage of hexagonal cells in the central cornea. For the pachymetry, ultrasonic pachymeter was used for central cornea in each twenty rabbits, and tonometry was performed forty rabbit's eyes to evaluate the intraocular pressure. Corneal endothelial cell density in New Zealand white rabbit was $3304{\pm}354\;cell/mm^2$. Coefficiency of variation was $45.8{\pm}2$ and the percentage of hexagonal cells in the central cornea was $44.4{\pm}11%$. The pachymetry of cornea was $346.1{\pm}21\;{\mu}m$ and tonometry of eye was $14.3{\pm}2\;mmHg$. It is considered that normal New Zealand white rabbit's pachymetry, specular microscopy and tonometry were useful basic data of rabbit's cornea for research and clinic in veterinary ophthalmology.

Reliability Analysis of Steel Fiber Reinforced Concrete Continuous Beams (강섬유 보강 철근콘크리트 연속보의 신뢰성 해석)

  • Yoo Han-Shin;Jang Hwa-Sup;Kwak Kae-Hwan
    • Journal of the Computational Structural Engineering Institute of Korea
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    • v.17 no.4
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    • pp.443-449
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    • 2004
  • Methods for mixing variable types of steel fibers have been developed recently to suppress outbreak of crack or to control the width of crack and improve the load resistible capacity at the same time. On the other hand, uncertainty by complex nature of destruction dynamics of steel fiber reinforced concrete(SFRC) is included. In this study, analysis of reliance considering uncertainty of SFRC beam is done. For this, intensity limit state model was proposed. Moreover, characteristic values about almost every kinds of probability variables were collected and presented according to home and foreign references. Process of improving uncertainty from the result of experiments by Bayseian updating method is also proposed on the purpose of offering better statistical characteristic values with more data in the new future. Fatigue fracture probability equation is proposed and needed statistical characteristic values were presented to analyze fatigue reliance

A Study on Shear Strength Test for FRP Girder of Filled Concrete (콘크리트 충진 FRP 거더의 전단재하 실험에 관한 연구)

  • Kwak, Kae-Hwan;Jang, Hwa-Sup;Kim, Woo-Jong;Kim, Hoi-Ok
    • Journal of the Computational Structural Engineering Institute of Korea
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    • v.21 no.4
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    • pp.365-373
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    • 2008
  • Fiber Reinforced Polymer, FRP has a light weight, a high tensile strength based on design, non-electronic, non-magnetic, and rust-resistant feature, etc and many researches are being conducted recently on FRP in the construction area. Among them, GFRP (Glass Fiber Reinforced Polymer) is excellent in price competitiveness and is widely being used. However, since GFRP has a relative low modulus of elasticity and causes excessive deflection, the section must be large to be used as a structural component and an investigative review must be carried out in design to set the limit for deflection by the use load. Therefore, in order to solve the mentioned technical problems, this study suggested a section of a module form such that application of a large-scale section is possible. Also, to secure the low rigidity of FRP, this study developed a new FRP+ concrete composite girder form that confined the concrete. To identify the structural movement of the developed FRP+ concrete composite girder, shear strength test was carried out.

DNA Microarray Analysis of Gene Expression in Antifungal Bacterium of Bacillus lentimorbus WJ5 (DNA microarray를 이용한 항진균 활성세균 Bacillus lentimorbus WJ5의 유전자 발현 분석)

  • 이영근;김재성;장유신;조규성;장화형
    • Korean Journal of Microbiology
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    • v.39 no.3
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    • pp.141-147
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    • 2003
  • The simultaneous expression levels of antifungal activity related genes was analyzed by DNA microarray. We constructed DNA chips contained 2,000 randomly digested genome spots of the antifungal bacterium of Bacillus lentimorbus WJ5 and compared its quantitative aspect with 7 antifungal activity deficient mutants induced by gamma radiation ($^{60}Co$). From the analysis of microarray hybridization by the Gene Cluster (Michael Eisen, Stanford Univ.), totally 408 genes were expressed and 20 genes among them were significantly suppressed in mutants. pbuX (xanthine permease, K222), ywbA (phosphotransferase system enzyme II, K393), ptsG (PTS glucose specific enzyme II ABC component, K877), yufO (ABC transporter (ATP-binding protein), K130l), and ftsY (signal recognition particle (docking protein), K868) were simultaneously down-regulated in all mutants. It suggested that they were supposed to be related to the antifungal activity of B. lentimorbus WJ5.

An Analysis on the Character Personality Modification of Spin-Off Animation (스핀오프 애니메이션에 나타난 캐릭터의 변화 분석)

  • Kong, Hyun-Hee
    • Cartoon and Animation Studies
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    • s.41
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    • pp.107-131
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    • 2015
  • This study is on the modification of characters' role and personality between spin-off animation and original works. For this, one of box office hits, (2011) and (2015) have been analyzed with Enneagram and Greimas' Actantial model. The result shows, firstly, the protagonist of spin-off work plays subject role in a new narrative comparing that he/she plays faithful helper's role in original work. Secondly, the protagonist's personality does not show big difference between in spin-off and in original. But, anyway, there's a small change that the secondary personality in original stands out as a primary personality in spin-off. Finally, the standing out personality of the protagonist tends to be 'achiever' or 'loyalist', those of stereotype successful animation characters. In conclusion, the role change according to new narrative seems to be unavoidable. And, the personality change is, too. However, the personality change is partial and it has continuity from the original considering the potential audiences' expectation.