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Desmutagenicity of the Enzymatic Browning Reaction Products Which Obtained from Prunus salicina (yellow) Enzyme and Polyphenol Compounds (재래종 황색자두효소 갈변반응 생성물의 돌연변이 억제작용)

  • Ham, Seung-Shi
    • Applied Biological Chemistry
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    • v.30 no.1
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    • pp.71-76
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    • 1987
  • The mutagenicity and desmutagenicity on enzymatic browning reaction products which obtained from prunes salicina (yellow) enzyme and polyphenol compounds were carried out. In the rec-assay on Bacillus subtilis strains H17 and M45, the enzymatic browning reaction products of pyrogallol, hydroxyhydroquinone, 3,4-dihydroxytoluene and catechol of $10^{-2}M$ did not showed mutagenicity. In the effects of various metal ions on the rec-assay, the enzymatic browning reaction products of pyrogallol showed mutagenic activity by $Fe^{3+},\;Mn^{2+},\;Zn^{2+},\;Ni^{2+}$ and $Al^{3+}$. In the enzymatic browning reaction products of hydroxyhydroquinone, $Cu^{2+},\;Mn^{2+}$ and $Pb^{2+}$ were effected in mutagenic action and the enzymatic browning reaction products of catechol was effected in mutagenic action by $Mn^{2+}$. In the DNA-breaking action of enzymatic browning reaction products of pyrogallol, hydroxyhydroquinone, 3,4-dihyroxytoluene and catechol did not show, DNA-breaking action. In the effects of various metal ions on the DNA-breaking action of enzymatic browning reaction products, $Cu^{2+}$ showed DNA-breaking action. In the mutagenicity test on Sal. typhimurium strains TA98 and TA 100 with S-9 mix, 4 kinds of browned substances did sot shove muragenicity, all the browned substances showed strong desmutagenic activity in the presence of benzo $({\alpha})-pyrene$ with S-9 mix.

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Detection of Irradiated Fruits Using the DNA Comet Assay (DNA Comet Assay를 이용한 과일의 방사선 조사 확인)

  • Oh, Kyong-Nam;Park, Jun-Young;Kim, Kyeung-Eun;Yang, Jae-Seung
    • Korean Journal of Food Science and Technology
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    • v.32 no.3
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    • pp.531-537
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    • 2000
  • The simple microgel electrophoresis of single cells, a 'comet assay', on fruit seeds enabled the rapid identification of irradiated fruits by comparing the intact non-irradiated cells and the damaged cells of irradiated fruits. Grapes and plums were irradiated with 0.1, 0.5, 0.7, 1.0 kGy and strawberries, peaches, apples, and nectarines were irradiated with only 1.0 kGy. Seeds were isolated, crushed, and the suspended cells were embedded in an agarose layer. After lysis of the cells, they were subjected to microgel electrophoresis for 2 minutes, and then stained. The DNA radiation-induced fragmentation of all the fruits stretched and migrated out of the cells forming a tail toward the anode giving the appearance of a comet, while the undamaged cells appeared as intact nuclei without tails. Grape and plum seeds irradiated at 0.5 kGy and higher showed significant increases in tail length. With increasing the irradiation doses, longer extention of the DNA from the nucleus toward the anode was observed. Strawberry, peach, apple, and nectarine seeds irradiated with 1.0 kGy also showed the longer tails than non-irradiated ones. DNA comet assay as a rapid and inexpensive screening technique could be an officially validated method for the detection of irradiated fruits.

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Fruit Piercing Moths Collected at an Orcgard Surrounded by forest in Gyeongnam Province (경남 산지 과수원에서 채집된 과실 흡수나방의 종류)

  • 박정규;신원교;김인곤;김창효
    • Korean journal of applied entomology
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    • v.27 no.2
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    • pp.111-116
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    • 1988
  • Fruit piercing moths were collected at every other at orchaed(16.5ha) planted with plum, peach and pear, and surrounded by forest in Gyeongnam province from jun to September in 1987. Four black light (BL) traps were lightened to attract the moths from sunset to sunrise and sweep net was also used to catch the moths on fruit and around fruit trees from 22 to 23 o'clock, 20 minutes per fruit tree species. Forty-one species, including 15 primary piercing species(PPS) and 22 secondary piercing species (SPS), from 3 families were collected and identified as fruit piercing moths. Among them, 16 species are newly recorded as fruit piercing moths in Korea. O. emarginata, L. juno, P. stuposa, C. lata and O. excavata were diminant species of PPS, comprising 86.7% of the whole PPS. Dominant species of SPS were A. ipsilon, M. turca, S. retorata, A. livida and T. oldenlandiae, comprising 80.5% of the whole SPS. The ratios of PPS to the whole fruit piercing moths collected by BL traps and net were sweep 15.2% and 79.7%, repectively. By sweep net L. juno, P. stuposa, and O. emarginata was also captured in a large numbers on peach, C. lata was on plum, and S. retorata was on pear.

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Oviposition Characteristics of Ricania sp.(Homoptera: Ricaniidae), a New Fruit Pest (과수의 신종해충인 날개매미충 일종의 산란특성)

  • Choi, Yong-Seok;Hwang, In-Soo;Kang, Tae-Ju;Lim, Ju-Rak;Choe, Kwang-Ryul
    • Korean journal of applied entomology
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    • v.50 no.4
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    • pp.367-372
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    • 2011
  • An unknown planthopper was discovered in 2010 in an apple orchard in Sinpoong-myun, Gongju-si and in a blueberry orchard in Deogsan-myun, Yesan-gun. This pest have arrived 4 or 5 years ago and the population density then rapidly increased. It was identified as Ricania sp. by Dr. Murray J. Fletcher. Adult Ricania sp. was found on 26 plants including blueberry, grape, peach, sumac, japanese angelica, jujube, kiwi, Rubus coreanus, apple, apricot, plum and chestnut. Females lay eggs on 1-year-old branches. The mean number of eggs laid on apple and plum were 18.7 and 15.3, respectively. The mean length of egg masses on apple and plum were 12.35 and 11.45 mm, respectively. The distance between eggs laid on apple and plum branchs were 1.3 and 1.5 mm, respectively. The mean number of eggs in the same length of egg mass on apple and plum branchs were 17.1 and 13.3, respectively.

Occurrence Patterns of Three Major Fruit Moths, Grapholita molesta, Grapholita dimorpha and Carposina sasakii, Monitored by Sex Pheromone in Plum Orchards (자두과원에서 성페로몬을 이용한 복숭아순나방, 복숭아순나방붙이, 복숭아심식나방의 발생 패턴)

  • Jeong, Sun-A;Sah, Lalit Prasad;Ahn, Jeong Joon;Kim, Young-Il;Jung, Chuleui
    • Korean journal of applied entomology
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    • v.51 no.4
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    • pp.449-459
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    • 2012
  • Fruit borers such as Grapholita molesta, G. dimorpha and Carposina sasakii are major pests of plum in Korea. Population densities and seasonal occurrence were monitored using the synthetic sex pheromone trap in Uiseong and Gyeongsan, two important major plum growing area in Korea. In 2010 and 2011, adults of G. molesta were caught from mid April with the peak of late April, and then undergone three more generations in Gyeongsan. Grapholita dimorpha appeared from late April and they showed three peaks until late September. Both Grapholita species occurred a few days later in Uiseong than in Gyeongsan where the latitude is $1^{\circ}$ lower. There was no difference of the phenological occurrence of C. sasakii between Gyeongsan and Uiseong, showing the same patterns of two or three peaks. Population size was in order of G. molesta, G. dimorpha and C. sasakii with 63:16:20 and 47:35:18 in Gyeongsan and 51:18:31 and 46:13:36 in Uiseong, in 2010 and 2011 respectively. There was no difference between the numbers of Grapholita species caught in the trap installed inside and outside of the orchards.

세계 3번째로 SRAM시대열어 - 256KD 램 보다 고부가가치 8월부터 생산수출

  • 한국발명진흥회
    • 발명특허
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    • v.10 no.8 s.114
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    • pp.64-64
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    • 1985
  • 금성반도체(대표 : 구자두)는 미국, 일본에 이어 세계 3번째로 첨단반도체제품인 CMOS 64K SRAM을 자체개발하는데 성공했다. 국내 최초로 개발된 금성반도체의 CMOS 64K SRAM은 우리나라의 반도체 기술수준을 선진국 수준으로 성큼 다가서게 했다. CMOS 64K SRAM은 NMOS의 256K DRAM에 비해 작동속도가 2배이상 빠를 뿐만 아니라 재충전이 필요없는 완전한 스태틱(static) RAM으로 대용량$\cdot$고속$\cdot$고신뢰성을 요하는 고성능 컴퓨터, 통신장비등 첨단 산업용 기기의 기억장치에 주로 사용된다.

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반도체, 맞춤시대 본격돌입 - 회로설계과정에 고객참여를 유도

  • 한국발명진흥회
    • 발명특허
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    • v.10 no.5 s.111
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    • pp.71-71
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    • 1985
  • 반도체, 컴퓨터, 통신기기 분야에서 최첨단 기술제품의 생산 공급을 통해 그동안 국내 전자산업을 선도해 온 금성반도체(대표 : 구자두)는, 지난해 6월 세계 3번째로 반주문형 초대규모 집적회로(VLSI)인 CMOS게이트 어레이를 개발하여 미국 엘에스아이 로직(LISLOGIC)사와 1억 5천만불의 수출계약을 체결함으로써 국내 최초로 주문형 반도체의 수출시대를 연데이어, 4월 10일 여의도 중심부 신한 빌딩 4층에 100여평 규모의 게이트 어레이 디자인 센터를 개관하여 특수한 반도체를 주문하는 고객이 동 제품의 회로 설계과정에 직접참여할 수 있도록 함으로써 수주활동을 본격화 하는 일대 전기를 마련하였다.

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Quality Characteristics and Antioxidant Activity of Puree Made with Plums Stored at Low Temperatures (저온저장 자두를 이용한 자두 퓨레의 품질특성과 항산화 활성)

  • Park, Myeongbin;Park, Samuel;Yeo, Chaeeun;Kim, Keono;Chun, Ik-Jo;Cho, Young-Eun;Sung, Jeehye
    • Journal of the Korean Society of Food Culture
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    • v.36 no.1
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    • pp.84-91
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    • 2021
  • The oriental plum (Prunus salicina) is one of the most popular fruits in Korea due to its sweet taste and pleasant aroma. The objective of this study was to analyze the quality characteristics and antioxidant activities of a puree made using plums stored at low-temperatures. The quality characteristics were determined by estimating total soluble solids (TSS, °Brix), titratable acidity (TA, % citric acid), TSS/TA ratio, pH, colors, sugar content, and sensory profiles. The antioxidant effects were estimated by total polyphenol and flavonoid content and radical scavenging activity. The results showed that the plum puree containing both the flesh and the peel had markedly higher red coloration (a⁎ value) than the puree without the peel, while yellow coloration (b⁎ value) and lightness (L⁎ value) were lower in the puree without the peel than with the peel. The sensory qualities including the plum taste and flavor, texture as well as overall acceptance showed no significant variation between the samples. Meanwhile, the total polyphenol/flavonoid content and radical scavenging activities were significantly improved by the presence of fruit peel and by frozen storage. These findings suggest that frozen plums could be a suitable ingredient for making a puree and concentrate for the food manufacturing industry.

In vitro Antifungal Activities of Fungicides against Japanese Plum Fruit Anthracnose Fungi (자두 탄저병균에 대한 살균제의 활성)

  • Jeong, Byeong-Ryong;Lee, Tae-Yi;Park, Min-Jung;Ha, Da-Hee;Chung, Jong-Bae;Lee, Yong-Se
    • Korean Journal of Environmental Agriculture
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    • v.37 no.1
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    • pp.34-40
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    • 2018
  • BACKGROUND: In order to select a fungicide that can effectively control anthracnose disease in Japanese plum fruit, mycelial growth inhibition effect and spore germination inhibition effect of six fungicides were tested in vitro against six isolates of Colletotrichum acutatum and five isolates of C. gloeosporioides that were isolated from diseased Japanese plum fruit. METHODS AND RESULTS: Inhibitory effects of fungicides on mycelial growth were investigated after inoculating each isolate on potato dextrose agar amended with four discriminatory concentrations of each fungicide for 7 days at $25^{\circ}C$. For spore germination inhibitory effect, each isolate of the Colletotrichum spp. was cultured in potato dextrose agar for 7-14 days at $25^{\circ}C$. After adjusting the concentration of spores of each isolate to $1{\times}10^6mL^{-1}$ by diluting with 0.025% PDB, the spore suspension was mixed with each fungicide (1:4, v/v), and $60{\mu}L$ aliquots were dispensed to sterile hole slide glass. Hole slide glasses were placed in a humidified box and incubated for 15 hours at $25^{\circ}C$. Then, spore germination was observed under an optical microscope. At recommended concentration of fungicide prochloraz manganese showed the highest mycelial growth inhibitory effect and dithianon showed the lowest mycelial growth inhibition. The $EC_{50}$ values for the inhibition of spore germination by dithianon and pyraclostrobin were $0.069-0.126{\mu}g/mL$ and $0.37-1.59{\mu}g/mL$, respectively. Although benomyl, prochloraz manganese, azoxystrobin, and tebuconazole did not inhibit the spore germination, they appeared to restrain mycelial growth by abnormal growth of germ tube and mycelium after germination. CONCLUSION: Dithianon seemed to have preventive effect. Prochloraz manganese, azoxystrobin, and tebuconazole were likely to have control effect. Pyraclostrobin is considered to have both preventive and control effect against anthracnose disease of Japanese plum fruit.

Identification, Larval Host Plant Range, and Damage of the Fruit Sucking Moths to the Major Fruit in Cheonnam Province (주요(主要) 과수(果樹)에 대(對)한 흡수(吸收)나방류(類)의 종류(種類), 유충(幼蟲)의 기주범위(寄主範圍) 및 피해상(被害相)에 관(關)하여(전남(全南) 과수지대(果樹地帶) 중심(中心)))

  • Kim, Kyu-Chin;Lee, Tae-Sik
    • Korean journal of applied entomology
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    • v.24 no.4 s.65
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    • pp.183-190
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    • 1986
  • Identification of fruit sucking moths, their larval host plant, and degree of damages to fruits were investigated in $1982{\sim}'83$ at two orchards in Cheonnam province. In this study, 4 families 30 species were collected as fruit sucking moths and among them 10 species are newly recorded in Korea as fruit sucking moth: Catocala electa(B.), Spirama helicina(H.), Erebus ephesperis(H.), Parallelia stuposa(F.), Simplicia niphona(B.), Marumba gaschkewitschii(B. et G.), Agrius convolvuli(L.), Thyatira batis (L.), and Spilosoma niveum(M.). Larval host plants of fruit sucking moths in the vicinity of the fruit orchard include Cocculus trilobus D., Albizzia julibrissin D., Pterocarya rhoifolia S., Jaglans mandshurica M., Solanum melongena L., Brassia campestris S., Rubus idaeus C., Thalictrum aquilegifol L., and Rhus succedanea L. As the damage on different varieties of the main fruit trees, plum was orderly Santa Rosa>Beauty, Apple was Chook>Hong Og, Peach was Choseng Baeg Do>Hwang Do>Baeg Do, and Grape was Campbell Early>Neo Mascat>Golden Queen. Seasonal occurrence of main fruit sucking moth Spirama retorta(C.) showed peak 3 times in early-July, late-July, early-September. Oraesia emerginata(F.) showed peak 3 times in late-July, early-August, early- September and Lagoptera juno(D.) showed peak only in late-July and Adrias tyrannus amurensis(S.) showed peak only in mid-July. Fruit sucking moths were immigrated from p.m. 6 to a.m. 2, with peak in p.m. 10 to p.m. 12.

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