• Environmental Mutagens and Carcinogens
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• v.8 no.1
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• pp.1-12
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• 1988

The effects of aphidicolin (APC), an inhibitor of DNA polymerase alpha, or 2', 3'-dideoxythymidine 5'-triphosphate (ddTTP), an inhibitor of DNA polymerase beta, on the repair of DNA damage induced by ethyl methanesulfonate (EMS) or bleomycin (BLM) were investigated in Chinese hamster ovary (CHO)-K1 cells. Three assays were employed in this study: unscheduled DNA synthesis, alkaline elution and alkaline sucrose gradient sedimentation. It was shown that APC or ddTTP inhibited DNA induced by EMS, and thus, the post-treatment with APC or ddTTP following EMS treatment was resulted in the more amount of unscheduled DNA synthesis, and the more accumulation of DNA single-stand breaks than the cells post-incubated without APC or ddTTP. While, in the BLM induced DNA repair, only ddTTP inhibited DNA repair induced by BLM. And thus, the groups post-incubated with or without APC after BLM treatment had the same value in the amount of unscheduled DNA synthesis and of DNA single-strand breaks, while post-treatment with ddTTP was resulted in the increased amount of unscheduled DNA synthesis and the increased DNA sin -strand breaks than the group without ddTTP. These results suggested that both of DNA polymerase $\alpha$ and $\beta$ participated in the repair of DNA damage induced by EMS, but in BLM-induced DNA repair, polymerase $\beta$ participated.ipated.

• Korean journal of communication and information
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• v.26
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• pp.259-289
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• 2004

In this study, the use of mainstream online news site and alternative online news site were compared in the aspects of users' characteristics, attitude on online news sites, and using pattern. A survey was conducted for 182 mainstream-only users, 46 alternative online news users, and 47 both sites users, Also, their traffic data of online news sites were analyzed during the 16th presidential election. As a result, it was found that both sites users had the highest political interest and the most progressive political position among the user groups. In the aspect of users' attitude, mainstream-only users were most positive to the mainstream online news site and both sires users were most positive and more involved in alternative online news site. But all user groups set higher credibility on alternative online news site than mainstream online news sire. In the comparison of user size, mainstream online news site has larger user size than alternative online site. However, the user royalty, such as time per person, pages per person, and visiting days per person, was lower than that of the latter. These results suggest thar small but differentiated news sires have royal users, and online news users be segmented according to news contents.

• Survey Research
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• v.7 no.1
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• pp.55-84
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• 2006

This study has classified development stages (Embryonic-Growth-Maturity) of mobile telecommunication industry based on Industry Life Cycle theory. There are two steps to be analyzed in this study, In the first step, cluster was investigated through cluster analysis using mobile density to categorize development stages of mobile telecommunication industry. In the second step, we compared on indexes of market structure, market efficiency and market performance to find out characteristics of each stage of development. The results are as follows. First, HHI is higher at embryonic stage than at growth and maturity stages, Second, ARPU(Average Revenue Per User) and RPM(Revenue Per Minute) are getting higher as the stages move on. Third, EBITDA margins, an index of market performance, is decreasing along the three stages. Finally, this study presents a clue to define the stage of development of mobile telecommunication industry and build a proper strategy for the market change.

• Journal of the korean academy of Pediatric Dentistry
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• v.30 no.1
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• pp.25-32
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• 2003

S. mutans is the most important causative bacteria of dental caries among the oral bacteria. S. salivarius is a normal inhabitant in the human oral cavity. Nine strains of S. salivarius in this study were isolated from the oral cavities of children and identified, and their effect on S. mutans and S. oralis was studied. 1. The mean weight of produced artificial plaque on the wires in the beaker was 204.9 mg in the culture of S. mutans only, whereas being reduced to 1.9 mg through 20.6mg in the combined culture of S. mutans and each S. salivarius isolate (p<0.05). The viable cell didn't show the difference between them after culturing. 2. When S. mutans was cultured in the media containing culture supernatant of each S. salivarius isolate in M17 broth, the mean weight of produced artificial plaque was 117.1 mg on the wires, whereas being 47.7 mg in the media containing culture supernatant of each S. salivarius isolate in M17 broth containing 5% sucrose. 3. The polymer produced by S. salivarius isolates was on the thin layer chromatography. 4. Inulin and levan didn't inhibit the formation of artificial plaque by S. mutans in the beaker test. These results suggested that fructan-producing S. salivarius isolates inhibited the formation of artificial plaque by S. mutans.

• Journal of the korean academy of Pediatric Dentistry
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• v.35 no.1
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• pp.73-82
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• 2008

Dental caries is an infectious disease caused by mutans streptococci, and is a primary etiologic agent of dental caries in humans. The molecular pathogenesis of mutans streptococcal-associated dental caries occurs in three phases. Firstly, S. mutans attaches to tooth surface via a cell surface adhesion termed antigen I/II. In the second phase, the glucosyltransferase(GTFs) synthesize polymers like glucans in the presence of sucrose. In the third phase, the multivalent glucans interacts with glucan binding proteins (GBPs) and they make dental plaque and accumulation of microorganisms. Many studies and clinical trials have indicated that a mucosal immune response to these antigens(Ag I/II, GTFs, GBPs) of S. mutans can influence the pathogenesis of dental caries. So these antigens can be important vaccine candidates for immunologic intervention against dental caries. In this study, we cloned the genes for GTFb, GTFc, GTFd from S. mutans GS-5 and did the nucleotide sequence analysis. And the recombinant proteins of GTFd and N-terminus of GTFd were expressed. Intact GTF which we get from this experiment can be used for antibody production specific for any GTF activity domain through animal experiment.

• Journal of Bio-Environment Control
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• v.26 no.4
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• pp.291-296
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• 2017

This study was conducted to evaluate the influence of early defoliation on photosynthesis and carbohydrate reserves when the source leaves of 'Fuji'/M9 apple (Malus domestica Borkh.) trees were removed during the growing period. Bud regrowth rates of 80%, 50% defoliation and non-defoliation treatments were significantly different 82.7%, 45.9% and 2.1% respectively at 30 days after treatment. In all treatments, sucrose and starch concentrations in remaining leaves decreased non-significantly during the 14-day period. No significant changes were observed for total soluble carbohydrates in non-defoliation and 50% defoliation. However, in 80% defoliated treatments, concentrations of sorbitol and total soluble carbohydrates in remaining leaves declined steadily during the 14-day period. It is thought that high sink strength increases the requirements of carbohydrate from remaining leaves more than non-defoliated. The concentrations of starch in the roots tend to decrease non-significantly as percentage of defoliation increased. Photosynthesis of remaining leaves was monitored during the 14-day period after partial defoliation treatments. Net photosynthetic rates (Pn) and stomatal conductance were significantly enhanced in the 80% defoliation. The observed photosynthetic enhancement following partial defoliation may have been due to the enhancement of osmotic potential in leaves. These results were estimated that increasing of photosynthetic rate in the partial defoliation is due to the sink carbohydrate requirements for the current year's secondary growth of buds.

• Korean Journal of Food Science and Technology
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• v.25 no.4
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• pp.386-390
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• 1993

Addition of starch syrup, table sugar, potato powder, skim milk powder an parched soybean powder to melon flesh was suitable as fermented melon base. The manufacturing process of fermented melon was as follows: Pasteurization for 10 min at $95^{\circ}C$, use of 1% starter culture, fermentation for 12 hours at $35^{\circ}C$ and ripening for 3 days at $8^{\circ}C$. The growth and acid production of Pediococcus acidilactici among several starter cultures were most active for the first 12 hours, but such activities were disappeared during ripening. In the case of Lactobacillus plantarum, the activities were not high during fermentation, which, however, increased during ripening. Throughout the whole manufacturing process, the fermented melon with a mixed culture of P. acidilactici and L. plantarum showed more cell number of each bacterium and higher titratable acidity than that with single cultures. Also P. acidilactici surpressed the growth of L. plantarum during ripening.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.15 no.3
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• pp.185-190
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• 1982

In utilization of small size red muscled fishes like mackerel, sardine, and filefish, mechanical dressing is usually required. The removal of dark muscle is also necessary to improve qualify of the product, which could hardly be done by mechanical process. As a method of separating dark muscle, specific gravity method using sugar solution was investigated in this study. And the effects of the level of specific gravity, the size and density of meat particles, and stability of meat particle float on the separation of dark muscle were discussed. From the results, effective specific gravity, in case of sucrose solution, ranged 1.067 to 1.072 for mackerel, 1.062 to 1.070 for sardine, and 1.072 to 1.077 for filefish, respectively. The maximum separation of more than $90\%$ was obtained at specific gravity of 1.075, 1.070, and 1.075 in cases of mackerel, sardine and filefish, respectively. The size of meat particles which were ground with 0.2cm orifice plate was adequate to yield $90\%$ separation or above. The meat particle float in the glucose solution began to precipitate within 5 minutes after separation while 25 minutes in case of sucrose solution. Lipids were also fairly removed by the dark muscle separation process.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.10
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• pp.530-540
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• 2020

Ecological momentary assessment (EMA) is a method that captures the current experience and behavior of subjects in real time in a natural environment. The data collected by EMA reveals alterations according to the time and context of experience and action. The Trier Inventory for Chronic Stress (TICS) is a tool that measures momentary stress, and is capable of measuring stress experienced in real life. This study was undertaken to verify the validity and reliability after translating the short form TICS into a Korean version. Totally, 89 police officers working at the police station in the capital participated in this study. The mobile application developed for this study was installed on their smartphones, and data were collected four times a day for seven consecutive days. Confirmatory factor analysis and content validity index (CVI) was applied to test the validity, and concurrent validity was evaluated by correlating with the occupational stress score. The model was validated by confirmatory factor analysis, and we obtained good reliabilities (I-CVI=0.83-1, RMSEA=0.104, SRMR=0.104, CFI=0.948, TLI=0.919, Cronbach's alpha=0.839). Our findings indicate the reliability, and validate use of the Korean tool for measuring momentary stress.

• KSBB Journal
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• v.21 no.4
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• pp.286-291
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• 2006

Various ${\beta}$-ionone resistant mutants were isolated from the wild-type red yeast Xanthophyllomyces dendrorhous KCTC 7704. Although the growth of X. dendrorhous KCTC 7704 was strongly inhibited at 0.025 mM ${\beta}$-ionone, one of the ${\beta}$-ionone resistant mutants isolated at 0.1 mM ${\beta}$-ionone by NTG mutagenesis showed rather 70% of relative survival at 0.15 mM ${\beta}$-ionone. Fermentation kinetics study with the mutant was carried out at $20^{\circ}C$ for 4 days in 300-mL baffled flasks. The mutant yielded up to 2.3-fold higher carotenoids content(viz. $1.2{\mu}g$ of total carotenoids per mg of dry cells) compared with the wild-type strain. The production of metabolites such as organic acids could be neglected. Studies on the kinetics with various carbon substrates revealed both an increase in final dry cell mass and a higher total carotenoids content in cell mass with glucose when compared to fructose or sucrose. As a further part of study, the effect of pH on the fermentation kinetics was investigated in glucose-limited chemostat at a dilution rate of $0.04h^{-1}$. When compared to steady-state kinetic parameters obtained at pH 4.0, a significant reduction in cell concentration at pH 3.0 and a lower carotenoids content at pH 5.2 were evident.