• Journal of Digital Convergence
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• v.15 no.7
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• pp.247-257
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• 2017

The purpose of this study was to measure the depression level of college students and to analyze the effects of college life stress and circadian rhythm on depression. A questionnaire survey was conducted to 720 students of college in Gwangju area from October 10, 2016 to October 31, and 687 of the collected questionnaires were analyzed. As this result, 34.5% of the students needed treatment for depression, and 71.6% of the students had mild depression. Second, depression was higher in female students, in higher grade students and students living apart from their parents. Third, evening activity type showed higher depression than morning activity type. Fourth, regression analysis revealed that depression was influenced by stress of value problem, circadian rhythm, academic stress, and getting a job stress. The results of this study suggest that students should be able to detect depression early and run a mental health program to relieve college life stress in college. However, this study is limited to some local college students and is not suitable for generalization. It is necessary to conduct research to compare university students and college students in the future.

• Journal of The Geomorphological Association of Korea
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• v.19 no.3
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• pp.109-121
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• 2012

Rock-surface temperatures were observed at a trachytic lava dome, called as Baeknokdam Northwest Face, in the summit area of Mt. Halla, Jeju Island, to examine the frequency and occurrence season of freeze-thaw cycles and the rate of temperature changes during a freezing period. Long-term measurements were recorded over 18 months from November 2006 to April 2008, at a 1-hour logging interval and rock depth of 1.5 cm. Both diurnal freeze-thaw cycles and effective freeze-thaw cycles appear in larger numbers on a south-facing rock face than a north-facing rock face. The diurnal cycles were dominantly observed on February and March for the south face and on November and April for the north face, respectively. The annual freeze-thaw cycles were confirmed in terms of the presence of seasonal freezing periods lasting from mid-November to mid-April for the south face and from early-November to late-April for the north face, respectively. The rate of decreasing temperatures during the seasonal freezing periods is larger on the north face than the south face. Notwithstanding the lower numbers of freeze-thaw events, the north face experiences a higher frost intensity since the number of hours below $-3^{\circ}C$ is larger on the north face than the south face. The number of freeze-thaw events and the duration of days with continuous sub-zero rock temperatures largely depend on the solar radiation controlled by the aspect of the monitored rock surfaces, and thus the high-frequency short-term frost cycle dominantly appears on the south face and the annual frost cycle on the north face, respectively.

• Proceedings of the Zoological Society Korea Conference
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• 1995.10b
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• pp.198.1-198
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• 1995

• Journal of Applied Biological Chemistry
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• v.53 no.3
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• pp.121-125
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• 2010

In the past 10 years, a lot of plant circadian rhythm researches have published in molecular biology and biochemistry. We discussed with published molecular studies of circadian clock and rhythmic genes in Arabidopsis, rice and algae. However past this studies are not sufficient to explain the whole rhythmic metabolism. Recently many researchers have concerned post-transcriptional, translational and post-translational modification of rhythmic proteins. From the view point of the high-throughput study, we could suggest the proteomic analysis with 2-DE gel electrophoresis and MS/MS techniques for the identification of modified proteins.

• 대한생식의학회:학술대회논문집
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• 2003.12a
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• pp.148.2-149
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• 2003

• Development and Reproduction
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• v.8 no.1
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• pp.57-64
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• 2004

This study was carried out to examine the expression of the circadian clock genes in the mouse ovary and testis at different developmental stages. Expression of Period1(Per 1), Period2(Per2), Period3(Per3), Cryptochrome1(Cry1), Cyptochrome2(Cry2), Clock Small and Prokineticin1 and Prokineticin2 receptor(Prok1r, Prok2r) genes in mouse ovary was explored by semiquantitative reverse transcription Polymerase chain reaction(RT-PCR) according to the developmental stage(post partum day; ppd 1, 7, 10, 21 and 35). Immunohistochemistry using PER1 antibody was also analyzed. The differential expression pattern of clock genes was presented according to stages of the mouse ovarian development (ppd 1, 7, 10, 21 and 35). In the cases of ovaries, at the starting point of follicle growth at ppd 7 and 10, the clock gene expression patterns were changed vastly. According to the developmental stages, the clock genes were highly expressed at ppd 7 and 10 in mouse testis also. Receptors for Prok2, the circadian output molecule of SCN, were also expressed in ovary at ppd 7 and in testis at ppd 1 and 7, respectively. Immnunohistochemical analysis of PER1 showed positive signals in the cytoplasm of oocytes and granulosa cells. The level or PER1 expression was increased in cells at the spermatogonia and the condensing spermatids. The expression pattern of Perl and localization of PER1 were showed similar patterns according to the developmental stages in ovary and testis. Taken together, it could be observed that the expression of clock genes was highly correlated with gonadal development and germ cell differentiation in mice. Therefore, in this study, circadian programming of the genes in the ovary and testis is strongly imposed across a wide range of core reproductive cycles and normal development of gametes. Although the existence of circadian genes is clearly investigated, further studies on the direct evidence is required for the understanding of the relationship between circadian genes and regulation of gonadal differentiation and germ cell development.

• Applied Microscopy
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• v.39 no.4
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• pp.333-340
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• 2009

In the present study, ultrastructural features of the mesophyll tissue have been investigated in Crassulacean acid metabolism (CAM)-performing succulent Orostachys. A large central vacuole and numerous small vacuoles in the peripheral cytoplasm were characterized at the subcellular level in both developing and mature mesophyll cells. The most notable feature was the invagination of vacuolar membranes into the secondary vacuoles or multivesicular bodies. In many cases, tens of single, membrane-bound secondary vacuoles of various sizes were found to be formed within the central vacuole. multivesicular bodies containing numerous small vesicles were also distributed in the cytoplasm but were better developed within the central vacuole. Occasionally, electron-dense prevacuolar compartments, directly attached to structures appearing to be small vacuoles, were also detected in the cytoplasm. One or more huge central vacuoles were frequently observed in cells undergoing differentiation and maturation. Consistent with the known occurrence of morphologically distinct vacuoles within different tissues, two types of vacuoles, one representing lytic vacuoles and the other, most likely protein storage vacuoles, were noted frequently within Orostachys mesophyll. The two types coexisted in mature vegetative cells but did not merge during the study. Nevertheless, the coexistence of two distinct vacuole types in maturing cells implies the presence of more than one mechanism for vacuolar solute sorting in these species. The vacuolar membrane is known to be unique among the intracellular compartments for having different channels and/or pumps to maintain its function. In CAM plants, the vacuole is a very important organelle that regulates malic acid diurnal fluctuation to a large extent. The membrane invagination seen in Orostachys mesophyll likely plays a significant role in survival under the physiological drought conditions in which these Orostachys occur; by increasing to such a large vacuolar volume, the mesophyll cells are able to retain enormous amounts of acid when needed. Furthermore, the mesophyll cells are able to attain their large sizes with less energy expenditure in order to regulate the large degree of diurnal fluctuation of organic acid that occurs within the vacuoles of Orostachys.

• Korean Journal of Microbiology
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• v.24 no.1
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• pp.32-37
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• 1986

In fermentation studies it revealed that Streptomyces sp. SMF 3001 started to synthesize extracellular alkaline protease from early exponential phase of cell growth. The biosynthesis of the alkaline protease was greatly induced by skim milk as a sola nitrogen source and further stimulation was observed under inorganic sulphur limited culture. However, it was found that the biosynthesis was apparently repressed by $NH_4^+$ and free amino acids, specially by cysteine. It was considered that the strain SMF 301 of Streptomyces sp. would produce the alkaline protease for the uptake of sulphur compounds from protein contained in the culture broth.

• Journal of Korean Society of Forest Science
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• v.94 no.6
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• pp.496-503
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• 2005

This study was conducted to investigate the influences of sapflow flux on soil water tensions and soil moisture content at the Abies holophylla plots in Gwangneung, Gyeonggido, from September to October 2004. The Abies holophylla had been planted in 1976 and thinning and pruning were carried out in 1996 and 2004. Sapflow flux was measured by the heat pulse method, and soil water tension was measured by tensiometer at hillslope and streamside. Time domain reflectometry probes (TDR) were positioned horizontally at the depth of 10, 30 and 50 cm to measure soil moisture content. All of data were recorded every 30 minutes with the dataloggers. The sapflow flux responded sensitively to rainfall, so little sapflow was detected in rainy days. The average daily sapflow flux of sample trees was 10.16l, a maximum was 15.09l, and a minimum was 0.0l. The sapflow flux's diurnal changes showed that sapflow flux increased from 9 am and up to 0.74 l/30 min. The highest sapflow flux maintained by 3 pm and decreased almost 0.0 l/30 mm after 7 pm. The average soil water tensions were low ($-141.3cmH_2O$, $-52.9cmH_2O$ and $-134.2cmH_2O$) at hillslope and high ($-6.1cmH_2O$, $-18.0cmH_2O$ and $-3.7cmH_2O$) at streamside. When the soil moisture content decreased after rainfall, the soil water tension at hillslope responded sensitively to the sapflow flux. The soil water tension decreased as the sapflow flux increased during the day time, whereas increased during the night time when the sapflow flux was not detected. On the other hand, there was no significant relationship between soil water tension and sapflow flux at streamside. Soil moisture content at hillslope decreased continuously after rain, and showed a negative correlation to sapflow flux like a soil water tension at hillslope. As considered results above, it was confirmed that the response of soil moisture tension to sapflow flux at hillslope and streamside were different.

• Proceedings of the Korean Society of Community Living Science Conference
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• 2009.09a
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• pp.106-106
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• 2009

영국에서는 농촌문제의 심각성과 범정부적 대책 마련에 대한 부처간 인식 공유가 증가함에 따라 2000년 "농촌백서"에 '농촌서비스 표준화(Rural Services Standard, RSS)'를 제정하였다. RSS는 농촌 주민의 삶의 질 제고와 복지 서비스 향상을 위해 달성할 주요 서비스의 National Minimum을 의미한다. 매년 RSS 운용에 대한 지속적인 모니터링을 통하여 내용을 매년 업데이트 하고 있다. 교육 및 아동서비스, 광대역 통신, 사회적 돌봄, 우체국 서비스, 보건, 인터넷 접속, 긴급 서비스, 교통의 8개분야 13개 서비스 기준을 설정 제시하고 있다. 우리의 경우도 농촌 주민의 삶의 질 향상을 위한 범정부적 투자가 이루어져 왔지만 농촌지역의 사회서비스 기준을 도시와 비교해 보면 아직도 부족한 부분이 많이 있다. 농촌 인구가 감소되고 있는 상황에서 지속적인 하드웨어 중심적 투자에 대한 의문제기와 함께 농촌 주민들이 최소한의 공적 서비스를 활용할 수 있는 기반이 되어야한다는 의견이 대립되고 있는 가운데 그 해결책을 찾기 위하여 '농촌 서비스 기준'을 설정하자는 논의가 추진되고 있다. 이에 본 연구에서는 인구 5만 이상 군지역에 속하는 경상북도 울진군 기성면 척산리를 중심으로 농촌 정주지원 서비스 요구사항을 분석하였다. 척산리에는 총가구 187호 363명이 거주하고 있으며 지난 3년간 인구 이동이 전혀 없었다. 주민들의 연령대는 50~60대가 가장 많으며 척산 3리가 가장 고령화 되어 있는 것으로 나타났다. 교통 환경은 좋은 편이었는데 기성공용버스 정류장에 10곳 이상의 차편이 있고 7번 국도가 있어 타 지역으로 이동도 용이하였다. 마을에 보건/의료를 담당하는 곳은 보건진료소뿐이었으며 잦은 외근과 구급약 부족 등으로 불편을 호소하고 있었다. 응급상황 발생시 수송 가능한 응급체계는 갖춰져 있지 않았고 노인들이 가장 많이 이용하는 물리 치료실은 일주일에 3번 개방하기에 불편하다고 하였다. 3층 건물의 복지회관이 있으나 장날(5일장; 1일, 5일)만 개방되어 이용에 한계가 있었고 마을주민을 위해 운영되는 프로그램은 없었다. 논농사 중심의 경제생활이었고, 부녀회와 청년회 등은 면단위 또는 행정리로 묶어서 운영하고 있었다. 주민들은 울진군 면소재지 중 척산리에만 약국이 없어 불편한 사항이 많기에 약국, 물리치료실의 상시 개방을 필요로 하였으며 장날을 이용한 노인대상 영화상영, 강연회 등 다양한 문화프로그램 운영을 희망하였다.