• Research in Plant Disease
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• v.25 no.4
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• pp.233-236
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• 2019

Citrus vein enation virus (CVEV), which was regulated as a quarantine virus in Korea, was firstly found on Jeju Island in 2017. In February 2018, a survey was carried out to determine the distribution of CVEV in the main commercial areas growing Citrus spp. and Poncirus trifoliata. The survey was performed at 203 groves in the southern Korean Peninsula and Jeju Island. CVEV infection was determined by reverse transcription polymerase chain reaction detection and sequencing. The coat protein (CP) gene sequences obtained from the CVEV-infected samples showed high similarities (more than 98%) to the previously reported CVEV CP sequences. In summary, CVEV was detected in 136 groves (67%), in which 85.4% of Citrus junos and 77.8% of Citrus unshiu were infected by CVEV. In Jeju Island, the infection rate of CVEV was relatively higher (90.6%). Our result revealed that CVEV has spread widely in Citrus and Poncirus in Korea. Based on the result, the Korean quarantine agency decide to exclude CVEV from quarantine in Korea.

• Journal of Plant Biotechnology
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• v.35 no.2
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• pp.147-153
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• 2008

Cry1Ac gene was introduced into chrysanthemum (Dendranthema grandiflorum Kitamura) 'Linneker Salmon' through Agrobacterium-mediated gene transformation to develop new lines showing resistance to tobacco cutworm (Spodoptera litura). Cry1Ac gene was transferred into chrysanthemum by Agrobacterium C58C1 containing pCAMBIA2301. After infection of Agrobacterium C58C1 with leaf segments, the segments were cultured on regeneration medium (MS + 1.0 mg/L BA + 0.5 mg/L IAA) containing 10 mg/L kanamycin for the first selection, on the same medium containing 20 mg/L kanamycin for the second selection, and on rooting medium (MS basal medium) containing 20 mg/L kanamycin for the third selection. Until the third selection, sixty nine plantlets (1.6%) were survived and rooted. Thirty six ones (0.8%) among them were confirmed as putative transformants with nptll gene by nptll primer PCR, and 35 (0.8%) of 36 ones as transformants with nptll gene and cry1Ac gene by Southern analysis. The gene transformation efficiency of cry1Ac gene was favorable with 0.8%. The resistance of tobacco cutworm (Spodoptera litura) in chrysan-themum transformant introduced cry1Ac gene was tested in green house. Three transformants were confirmed to have resistance to tobacco cutworm.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.15 no.6
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• pp.3455-3459
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• 2014

This paper reports the design and experiments for a high drive force of projectile in a coil gun system. Currently, the coil gun has been studied to apply an electromagnetic launcher. A coil gun launches a projectile by the attractive magnetic force of the electromagnetic coil. The drive force of projectile is proportional to the magnetic force generated by the electromagnetic coil. The current affects the life of the coil and the current limit exists. Therefore, the coil gun design, which does not exceed the current limit and the magnetic forces are at the maximum, is required. For this purpose, this study calculated the magnetic flux density and forces of the coil gun system and determined the current limit of the coil using the Onderdonk's equation. Based on the design result, a prototype was manufactured and an experiment was conducted to measure the muzzle velocity of the projectile. The fired projectile was analyzed using a CCD camera, and the muzzle velocity was 21m/s. In addition, a comparison of the experimental value and analysis value using commercial electromagnetic analysis software MAXWELL revealed an error of approximately 9.5%.

• International Journal of Highway Engineering
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• v.13 no.4
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• pp.167-175
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• 2011

Evaluation of fuel consumption for the various road condition and vehicle type is necessary to perform the economic analysis of road construction which is important for the efficient design and management of road. Economic analysis of road should consider the social cost which can be divided into agency cost including initial construction expense, maintenance cost, and so on, and user cost consisting of vehicle operating cost, congestion cost, etc. Since vehicle operating cost depends on the traffic volume, fuel consumption that is a major part of vehicle operating cost will change by traffic volume as well. Fuel consumption is significantly affected by vehicle speed and road condition, especially the roughness. Thus, fuel consumption should be evaluated in terms of road condition, which is not currently considered. In this study, the estimation model of fuel consumption for the passenger cars in Korea has been developed by considering the road condition. First, the relationship between vehicle speed and fuel consumption that is used to calculate the vehicle operating cost for investment evaluation of transportation facility and the initial feasibility study of road construction was investigated. Second, with the consideration of road roughness, fuel consumption of the passenger car was measured. From the measurement, it was found that fuel consumption increased by $80m{\ell}$ per 100km driving as the roughness increased by 1m/km. Therefore, it is recommended that for the economic analysis of road design and management, the fuel consumption should be a function of road roughness.

• Journal of the Korea Society of Computer and Information
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• v.16 no.1
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• pp.31-38
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• 2011

A RAM-based Neural Net is a weightless neural network based on binary neural network. 3-D neural network using this paper is binary neural network with multiful information bits and store counts of training. Recognition method by MRD technique is based on the supervised learning. Therefore neural network by itself can not distinguish between the categories and well-separated categories of training data can achieve only through the performance. In this paper, unsupervised learning algorithm is proposed which is trained existing 3-D neural network without distinction of data, to distinguish between categories depending on the only input training patterns. The training data for proposed unsupervised learning provided by the NIST handwritten digits of MNIST which is consist of 0 to 9 multi-pattern, a randomly materials are used as training patterns. Through experiments, neural network is to determine the number of discriminator which each have an idea of the handwritten digits that can be interpreted.

• Parasites, Hosts and Diseases
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• v.35 no.4
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• pp.277-282
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• 1997

The only observed morphological difference between Spirometra erinqsei and S. mcnsonoides is the uterine shape of the mature proglottid. Two species of worms are thought to be evolutionarily closely related. Biomolecular colnparison of the ho worms by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis was conducted to observe the genetic distance. The 285 rDNA, mitochondrial cytochrome c oxidase subunit I (mCOI), and ribosomal internal transcribed spacer 1 (ITSI) fragments were obtained from the worms by PCR. The PCR products were cleaved by 5 four-base pair restriction enzyme combinations (Msp I, Hae III, Alu I, Cfo I, Rsa I) , electrophoresed and analyzed with PAUP 3.1.1. The fragment Patterns or 285 rDNA and Lni demonstrated that two worms were in identical systematic tree with bootstrap number 94 and 100, respectively As for mCOI, bootstrap number was 74 in a different tree. Above results are indicative of recent common ancestry between S. etinocei and S. mansonoides.

• Journal of Korean Society of Steel Construction
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• v.23 no.1
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• pp.41-49
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• 2011

A long-span (more than 8m) plastic greenhouse is currently being used in farms due to its magnified benefits, such as the convenience of the farming equipment used, and the land usage efficiency. In this study, the reinforcing effects of the use of a pretension tie were shown. In a previous study, tests for a 6.5m single-span-type greenhouse announced by Rural Development Administration were carried out. The tests of symmetric and eccentrics now loading by the sun and wind were conducted for the 10.2m span with a ${\phi}48.1{\times}2.1$ section in this study, after which the load-deflection relationship was compared for the cases of reinforcement with a tie and without a tie. The results of the symmetric snow loading test showed that the strength increased by 68~93% in the case of the specimen with a tied arch. The failure mode of the specimen without a tie tended to be that with a sway failure mechanism, and that of the reinforcement specimens with a tie tended to be that with an arch buckling mechanism. The results of the eccentric snow loading test showed that the strength of the specimen with a tie increased by 10~20% compared to that of the specimen without a tie. For the failure mode of the latter, a combined failure mechanism was adapted, although the failure mode of the tied specimens tended to be that with an arch buckling mechanism.

• Journal of the Korean Wood Science and Technology
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• v.39 no.6
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• pp.469-480
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• 2011

In this study, to determine the ligninase activity related to the PCBs degradation of Ceriporia sp. ZLY-2010, the protein contents and manganese peroxidase (MnP) and laccase activities during cultivation on shallow stationary culture (SSC) medium were observed. 4 PCB congeners were selected depending on the number of chlorine substituted on biphenyl. Furthermore, to examine the effects of cytochrome P450 monooxygenase, the inhibition of cytochrome P450 monooxygenase was evaluated by measuring the biodegradation rate when inhibitor such as 1-aminobenzotriazole was added. The extracellular protein contents were affected by PCB congeners in culture media. The total protein in the culture medium showed the biggest differences between the samples containing 2,2',4,4',5,5'-hexachlorobiphenyl and the control. On the other hand, MnP and laccase activity showed dominant increases within samples containing 4,4'-dichlorobiphenyl and 2,3',4',5-tetrachlorobiphenyl. Cytochrome P450 monooxygenase was inhibited by adding inhibitor, 1-aminobenzotriazole in low concentration. Only 2.73% of 2,3',4',5-tetrachlorobiphenyl was degraed on day 1, and biodegradation of 2,2',4,4',5,5'-hexachlorobiphenyl was inhibited as well, showing about 20% of biodegradation rate.

• Journal of the Korean Ceramic Society
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• v.31 no.5
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• pp.513-519
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• 1994

Alumina composite was fabricated by introducing tetragonal ZrO2 in alumina composite of $\alpha$-alumina reinforced with in-situ formed La-$\beta$-aluminate (LaAl11O18). The powder mixture of composition (100-x)[88 Al2O3+(La2O3+11 Al2O3)]+x vol% ZrO2 was prepared with x from 0 to 40. Dense composites fabricated by hot-pressing exhibited bending strength up to 1200 MPa. The gain growth inhibiting effect of ZrO2 grains and La-$\beta$-aluminate platelets, and possibly the stress-induced phase transformation of ZrO2 have resulted in the high bending strength. The fracture toughness of the composite also increased up to 8.5 MPa.m1/2. The enhancement in toughness can be explained by transformation and microcrack toughening of ZrO2 and by crack deflection and bridging of La-$\beta$-aluminate platelets and ZrO2.

• Anxiety and mood
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• v.4 no.2
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• pp.142-147
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• 2008

Objective : Evidence from recent studies supports the role of genetic factors in the development of Posttraumatic Stress Disorder (PTSD). The primary aim of this study is to investigate the association between the dopamine D2 receptor (DRD2) TaqI A polymorphism and PTSD. The second aim is to examine the association between the DRD2 TaqI A polymorphism and clinical symptoms in patients with PTSD. Methods : We recruited 189 Vietnam veterans for participation in this study, among whom 99 were PTSD patients and 90 were control subjects. The presence of the DRD2 TaqI A polymorphism was determined by polymerase chain reaction (PCR). Several standardized research scales were used in the clinical assessment of PTSD, including the Combat Exposure Scale (CES), Clinician Administered PTSD Scale (CAPS), Beck Depression Inventory (BDI), and Clinical Global Impression (CGI). Results : There was no significant difference in the distribution of the DRD2 genotype, frequency and prevalence of the A1 allele, or the frequency of heterozygotes between the patients with PTSD and the controls. In the PTSD group, the patients with the A1 allele (A1A1, A1A2) scored higher on the CAPS-total (p=0.044), CAPS-avoidance symptoms (p=0.016) and BDI (p=0.024) than those without the A1 allele (A2A2). Conclusion : We could not find an association between the dopamine D2 receptor (DRD2) TaqI A polymorphism and PTSD. However, the A1 allele of DRD2 seemsto influence avoidance symptoms in patients with PTSD.