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Visual Cell : Image Analysis and Visual Retrieval System for Biology Cell Image Bigdata (Visual Cell : 바이오세포 이미지 빅데이터를 위한 이미지 분석 및 시각적 검색 시스템)

  • Park, Beomjun;Jo, Sunhwa;Lee, Suan;Shin, Jiwoon;Yoo, Hyuk Sang;Kim, Jinho
    • The Journal of Bigdata
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    • v.4 no.1
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    • pp.53-61
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    • 2019
  • The extracellular matrix, which provides the structural and biochemical support of surrounding cells, is a cell physiological modulator that controls cell division and differentiation. In the bio sector, the company produces Scapold, a three-dimensional support for tissue engineering, and cultivates stem cells in the produced Scapold to be transplanted into animals to assess tissue regeneration. This depends on components such as collagen in the tissue. Therefore, it is very important to identify the inclusion rate and distribution of components in the tissue, and the data are obtained by analyzing the color of the dyed tissue image. The process from image collection to analysis is costly, and the data collected and analyzed are managed in different formats by different research institutions. Therefore, data integration management and analysis results search are not being performed. In this paper, we establish a database that can manage relevant bigdata in an integrated manner, and propose a bio-image integrated management and retrieval system that can be searched based on color, an important analytical measure in this field of study.

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Mass-production of Eleutherococcus seoulensis Seedlings Through Somatic Embryogenesis (체세포배 형성을 통한 서울오갈피(Eleutherococcus seoulensis) 묘목의 대량생산)

  • Lee, Su-Gwang;Kang, Ho-Duck
    • Journal of Korean Society of Forest Science
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    • v.98 no.6
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    • pp.719-725
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    • 2009
  • This study was conducted to establish the optimal condition for acclimatization from somatic embryos of Eleutherococcus seoulensis. Torpedo-shaped embryos of Eleutherococcus seoulensis were cultured on 1/3 MS and WPM media supplemented with $GA_3$ (3.0, 5.0 mg/L) for 4 weeks. Plentlets were transferred to 1/2 SH solid medium with 1.0 mg/L $GA_3$ and 0.2% activated charcoal for shoot and root elongation and them elongated plantlets further developed on 1/2 SH medium for 4 weeks. Developed plantlets further elongated into well-shaped leaf and root system on 1/3 SH medium under ventilation condition for 4 weeks. Plantlets grew normally on 1/3 SH basal medium, were acclimated on various soil. Survival frequency of plantlets was influenced by soil type(peatmoss+perlite, perlite, soil on Nam mountain). The highest survival rate to soil was more than 70% when plantlets were 1/3 SH medium under ventilation condition in Nam mountain soil. These results indicate that the systematic procedure of plant production in Eleutherococcus seoulensis could be practically applicable for mass propagation.

Plant Regeneration via Secondary Somatic Embryogenesis and Acclimatization in Panax ginseng (장뇌삼의 2차 체세포배 발생을 통한 식물체 유도 및 순화)

  • Lee, Su-Gwang;Kim, Ji-Hee;Kang, Ho-Duck
    • Journal of Korean Society of Forest Science
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    • v.97 no.1
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    • pp.127-133
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    • 2008
  • This study was conducted to establish the optimal condition for plant regeneration and acclimatization from somatic embryos of Panax ginseng. Cotyledon segments of Panax ginseng produced primary and secondary somatic embryos when cultured on MS and WPM media supplemented with 7% sucrose. To induce plantlet conversion, cotyledonary somatic embryos were cultured on WPM solid medium with $GA_3$ at various concentrations (1~30 mg/L) for 4 weeks. Plantlets were transferred to 1/2 WPM solid medium with $GA_3$ at various concentrations (0~5 mg/L) and 0.5% activated charcoal for shoot and root elongations. Elongated plantlets further developed into well-developed leaf and root system on 1/3 SH medium with 0.5% activated charcoal under ventilation condition for 5 months. The highest survival rate to soil was 75% when plantlets were regenerated on 1/3 SH medium without sucrose under ventilation condition.

열 화학기상증착법을 이용한 BCN 박막의 합성과 전기적 특성 분석

  • Jeon, Seung-Han;Song, U-Seok;Jeong, Dae-Seong;Cha, Myeong-Jun;Kim, Seong-Hwan;Kim, Yu-Seok;Lee, Su-Il;Park, Jong-Yun
    • Proceedings of the Korean Vacuum Society Conference
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    • 2014.02a
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    • pp.388.2-388.2
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    • 2014
  • 최근 그래핀 연구와 더불어 2차원 구조의 나노소재에 대한 관심이 급증하면서 육각형의 질화붕소(hexagonal boron nitride; h-BN) 박막(nanosheet) [1]이나 붕소 탄화질화물(boron caronitride; BCN) 박막 [2,3]와 같은 2차원 구조체에 대한 연구가 활발히 진행되고 있다. 그 중 BCN은 반금속(semimetal)인 흑연(graphite)과 절연체인 h-BN이 결합된 박막으로 원소의 구성 비율에 따라 전기적 특성을 제어할 수 있다는 장점이 있다. 따라서 다양한 나노소자로의 응용을 위한 연구가 활발히 진행되고 있다. 본 연구에서는 폴리스틸렌(polystyrene, PS)과 보레인 암모니아(borane ammonia)를 고체 소스로 이용하여 열화학기상증착법을 이용하여 BCN 박막를 SiO2 기판 위에 직접 합성하였다. SEM과 AFM 관측을 통해 합성된 BCN 박막을 확인하였으며, RMS roughness가 0.5~2.6 nm로 매우 낮은 것을 확인하였다. 합성과정에서 PS의 양을 조절하여 BCN 박막의 탄소의 밀도를 성공적으로 제어하였으며, 이에 따라 전기적인 특성이 제어되는 양상을 확인하였다. 또한 합성온도 변화에 따른 BCN 박막의 전기적인 특성이 제어되는 양상을 확인하였다. 추가적으로 같은 방법을 이용하여 BCN 박막을 Ni 위에서 합성하여 SiO2 기판위에 전사 하였다. 합성된 BCN 박막의 구조적 특징과 화학적 조성 및 결합 상태를 투과전자현미경(transmission electron microscopy), X-선 광전자 분광법(X-ray photoelectron spectroscopy)을 통해 조사하였다.

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Biotransformation of trans,trans-farnesol by Wood Rot Fungi (목재부후균에 의한 trans,trans-farnesol의 생물변환)

  • Kim, Young-Hun;Lee, Su-Yeon;Park, Mi-Jin;Choi, In-Gyu;Lee, Jae-Won
    • Korean Journal of Microbiology
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    • v.48 no.1
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    • pp.37-41
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    • 2012
  • In this study, we screened and evaluated possibility of wood rot fungi as biocatalyst for biotransformation of sesquiterpenes. Screening were performed to select the most promising microorganisms with ability to biotransformation the substrate trans,trans-fanesol. Trans,trans-farnesol which is synthesized precursor of sesquiterpenes was used for resistance test on 19 of wood rot fungi. From the 19 tested wood rot fungi, 5 were selected by resistance test on different concentration of trans,trans-fanesol. Biotransformation was performed with selected wood rot fungi on liquid culture. The metabolites detected by GC-MS analysis were nerolidol for Laetiporus sulphureus var. miniatus (jungh) Imaz and eicosane for Coriolus versicolor (L.Fr) Prlar and isoborneol for Fomitopsis pinicola and isocyclocitral for Lampteromyces japonicas. As the results, wood rot fungi could be potential biocatalyst for biotransformation of sesquiterpenes.

The Dynamic Characteristics and Serviceability of Long Span Multi-purpose Hall (장스팬 다목적 홀의 동적특성과 사용성)

  • Lee, Sung-Min;Choi, Chui-Kyung;An, Young-Ki;Lee, Soo-Gon
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.7 no.2
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    • pp.105-113
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    • 2003
  • Because structural systems are becoming lighter and more flexible and have lower natural frequencies and dampings than before, coordinated rhythmic activities such as dancing, audience participation in arenas or concert halls, and aerobics result in undesirable levels of vibration. For rhythmic activities, it is resonant or near resonant behavior that result in significant dynamic amplification and hence human discomfort. The most rational design strategy is to provide enough of a gap between the natural frequency of a floor system and the dominant frequencies excited by planned human activities to assure reasonably that resonance will not occur. For the case study the vibration measurements were performed at the floor of a long-span multi-purpose hall during the rock concert of popular singer.

STM Study of Low Dimensional Nanostructures Formed by Adsorption of Dipyrromethane-trimer Molecules on Graphite Surface (흑연 표면에 형성된 dipyrromethene-trimer 분자의 저차원 나노구조의 주사 터널링 현미경 연구)

  • Son, S.B.;Lee, S.J.;Hahn, J.R.;Shin, J.Y.;Dolphin, D.
    • Journal of the Korean Vacuum Society
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    • v.17 no.5
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    • pp.375-380
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    • 2008
  • We have investigated the low-dimensional nanostructures produced by adsorption of triangular Co coplexed dipyrromethane(DPM-trimer, Fig. 1) on graphite surface by using scanning tunneling microscope. DPM-trimer deposition on the graphite surface leads to the formation of long 1-D molecular wires and 2-D hexagonal patterns. We analyzed the heights and structures of 1-D molecular wires and 2-D hexagonal patterns. The 1-D molecular wires were formed 'edge-on' alignments on graphite surface result of continuos $\pi-\pi$ stacking interactions. The other case of 2-D hexagonal patterns were formed 'face-on' alignments on graphite surface.

Experimental Study of the Changing Characteristics of Geocell with Load Carrying capacity (지오셀 특성 변화에 따른 하중지지력 연구)

  • Hong, Seungrok;Choi, Jinwook;Yoo, Chungsik;Lee, Daeyoung;Lee, Suhyung;Yoo, Inkyoon
    • Journal of the Korean Geosynthetics Society
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    • v.12 no.3
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    • pp.1-13
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    • 2013
  • This paper presents the results of a laboratory investigation of the porous pavement substructure effect when reinforced with geocell. In order to analyze load carrying capacity of Geocell, a series of 9 reduced-scale laboratory tests was performed, changing the type, thickness, diameter of Geocell. The results of the analyses indicated that the bearing capacity of the reinforced Geocell increases much more than the non-reinforced Geocell and load carrying capacity was considered to be insignificant according to the type of Geocell. It was also found that the most supportive effects appeared as 30 cm in diameter and 1.8mm in thickness.

Development and Test of Line-Telemetry DPS for KSLV-I Upper Stage (나로호 상단부 Line-Telemetry 데이터처리시스템 개발 및 시험)

  • Kim, Kwang-Soo;Lee, Soo-Jin;Chung, Eui-Seung
    • Aerospace Engineering and Technology
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    • v.10 no.1
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    • pp.107-115
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    • 2011
  • The line-telemetry data processing system is necessary for monitoring the status of each onboard systems of KSLV-I upper stage during the ground tests and launch preparation. The mission of line-telemetry system is to provide reference telemetry data and to monitor the status of upper stage. The line-telemetry data processing system consists of a PCM acquisition/processing server, a system management server, and 9 monitoring consoles. In this paper, we will describe the overview of onboard remote measurement system, the design of the line-telemetry data processing system, anomaly setup information for indicating alarm signal in case of abnormal occurrence, and the result of the ground test and flight test.

Synthesis of Fluorine-Containing Water-Soluble Polyurethane with Environmental Affinity (환경친화적 수용성 불소계 폴리우레탄의 합성)

  • Lim, Hyejin;Park, In Jun;Lee, Soo-Bok;Lee, Yongtaek
    • Clean Technology
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    • v.5 no.2
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    • pp.37-44
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    • 1999
  • The influence of the reactant composition on the surface free energy and on the dispersity in water was investigated by using the fluorine-containing water-soluble polyurethane synthesized with the fluorine-containing diol and hydrophilic diol. The diol donating ionic characteristics was more effective than polyol, and stable emulsion with the diameter of 610~310 nm was obtained in the range of 0.3~0.7 molar ratio of the diol to polyol. According to the increment of fluorine-containing compound up to 10 wt %, the surface free energy of polyurethane was dramatically decreased from 22.3 to 12.6 dyn/cm, and the diameter of water dispersed polyurethane increased from 380 to 860 nm due to the strong interaction between polymer molecules because fluorine segments are arranged on the surface of molecules. The macromonomer with perfluoroalkyl group was more effective in complement of the weak point of water-soluble polyurethane, which is a decrease of hydrophobicity, because of strong hydrophobicity.

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