• Title/Summary/Keyword: 이상개체파악

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Comparative studies of the five edible mountain vegetables by DNA marker fingerprinting (DNA marker 지문법에 의한 취나물 5종 (청옥취 , 개미취 , 참취 , 수리취 , 곰취)의 비교연구)

  • 유기억
    • Korean Journal of Plant Resources
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    • v.9 no.3
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    • pp.305-310
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    • 1996
  • Five edible mountain vegetables(Saussurea sp. Aster tataricus, A. scaber. Synurus deltoides, Ligularia fischeri) were investigated on the basis of amplified DNA polymorphisms resulted from PCR (polymerase chain reaction) analysis. The sampled plants consisted of 38 individuals in 5 taxa. Only 10 primers out of 62 primers (60 random [10-mer] primers, two 15-mer [M13 core sequence, and (GGAT) sequence]) tested gave rise to polymorphisms in all of the tested plants, producing 176 DAN fragments amplified. Intraspecific polymorphisms found in each taxa showed intraspecies constancy (31.1-61.1%) in the banding patterns of individual plants: Saussurea sp. 31.1%, 15 bands, Aster tataricus, 40.9%, 18 bands, A. scaber. 38.5%, 15 bands. Synurus deltoides, 34.7%, 17 bands, and Ligularia fischeri, 61.1%, 22 brands, respectively. All five species were well classified from each other at the 0.93 level of similarity index value. Intraspecific and interspecific variations were appeared at the levels ranging from 0.62 to 0.99. Based on these results, our PCR analyses support the previous data derived from external morphology of the 5 edible mountain vegetables, but very low levels o intraspecific variations were detected in all of these taxa.

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Implementation of Policy based In-depth Searching for Identical Entities and Cleansing System in LOD Cloud (LOD 클라우드에서의 연결정책 기반 동일개체 심층검색 및 정제 시스템 구현)

  • Kim, Kwangmin;Sohn, Yonglak
    • Journal of Internet Computing and Services
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    • v.19 no.3
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    • pp.67-77
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    • 2018
  • This paper suggests that LOD establishes its own link policy and publishes it to LOD cloud to provide identity among entities in different LODs. For specifying the link policy, we proposed vocabulary set founded on RDF model as well. We implemented Policy based In-depth Searching and Cleansing(PISC for short) system that proceeds in-depth searching across LODs by referencing the link policies. PISC has been published on Github. LODs have participated voluntarily to LOD cloud so that degree of the entity identity needs to be evaluated. PISC, therefore, evaluates the identities and cleanses the searched entities to confine them to that exceed user's criterion of entity identity level. As for searching results, PISC provides entity's detailed contents which have been collected from diverse LODs and ontology customized to the content. Simulation of PISC has been performed on DBpedia's 5 LODs. We found that similarity of 0.9 of source and target RDF triples' objects provided appropriate expansion ratio and inclusion ratio of searching result. For sufficient identity of searched entities, 3 or more target LODs are required to be specified in link policy.

Ecological Studies of Wild boars(Sus scrofa) in Yeongwol Hanbando Wetland Inferred through DNA Analysis of Non-invasive Samples (비침습 샘플 DNA 분석으로 유추한 영월 한반도습지 내 멧돼지(Sus scrofa)의 생태 연구)

  • Kim, Minkyung;Lee, Sang-im;Park, Hyomin;Lee, Sangdon
    • Journal of Environmental Impact Assessment
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    • v.29 no.3
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    • pp.230-238
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    • 2020
  • This study inferred the ecology of habitat use of the wild boars (Sus scrofa) in Yeongwol Hanbando wetland through DNA analysis using non-invasive samples of hairs. From November 2018 to May 2019, hair samples were collected from rubbing trees and hairtraps within the Hanbando wetland (2.772 ㎢). We extracted DNA from the hair samples and conducted PCR to verify the species and identify sex of the individuals. In addition we analysed 6 microsatellite markers to identify individuals and genetic relationship among the pairs of individuals. A total of 16 boar hairs were sampled, which turned out to be from 10 individual (7 females and 3 males) boars. We found that 9 pairs, out of 45 possible pairs, were most likely to be relatives. The result from kinship data and the location of the sampled hairs suggest that wild boars in this area live as family groups that consist of a mother and her offspring, which is consistent with known habits of wild boars. It is needed to include more samples and microsatellite markers for better precise estimation of kinship among the boar individuals.

A Characteristics and Management Plan of Elaeocarpus sylvestris var. ellipticus Population Distributed in Munseom(island), a Natural Monument (천연기념물 문섬의 담팔수 개체군 특성 및 관리방안)

  • Choi, Byoung-Ki;Lee, Ho-Sang;Seo, Yeon-Ok;Choi, Hyung-Soon;Yang, Ju-Eun;Song, Kuk-Man;Song, Gwan-Pil
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.39 no.1
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    • pp.37-42
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    • 2021
  • Munseom (Island) is known as the largest native Elaeocarpus sylvestris var. ellipticus in Korea. Recently, disease damage from the E. sylvestris var. ellipticus community has been reported in Munseom. This study was conducted to understand the damage situation and growth characteristics of the E. sylvestris var. ellipticus population in Munseom. The purpose of this study was to evaluate the spontaneous regeneration potential of the community by grasping the characteristics of the population of surviving E. sylvestris var. ellipticus individuals, and to discuss ways to restore native habitats. A total population survey was conducted to determine the distribution status of E. sylvestris var. ellipticus individuals. The field survey carefully recorded the height of tree, DBH, DRH, and growing status, along with GPS location information of the individual. The growth status of E. sylvestris var. ellipticus individuals distributed in Munseom and the characteristics of tree height, DBH, DRH of each individual were analyzed. The total number of E. sylvestris var. ellipticus populations identified in Munseom was 293. The dense E. sylvestris var. ellipticus community reported in 2005 has been identified as being greatly damaged or damaged. In particular, the damage was more serious in mature trees with a height of 6m or more forming the canopy layer in the forest, and it was confirmed that 80.6% of the mature trees died. In the growth characteristics of the old-growth tree, which is an indicator of the health of the forest based on DRH, 75.4% died or the growth condition was found to be poor. In order to restore the native habitat of E. sylvestris var. ellipticus in Munseom, conservation efforts such as pest control, preservation of mature trees, control of the territory of young trees, and reintroduction after ex-situ transplantation etc. are considered necessary.

The relationship between the population characteristics and physical habitat of Manchurian trout(Brachymystax lenok tsinlingensis) in the Geybangcheon stream (계방천에 서식하는 열목어의 개체군 특성 및 물리적 서식환경과의 상관관계)

  • Ko, Min Seop;Choi, Jun Kil;Lee, Hwang Goo
    • Korean Journal of Environmental Biology
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    • v.39 no.1
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    • pp.108-118
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    • 2021
  • The purpose of this study was to provide baseline ecological data for the conservation of the Manchurian trout habitat through the investigation of the growth status of Brachymystax lenok tsinlingensis, and Pearson's correlation analysis (PCA) between the B. lenok tsinlingensis population and the use of the land around Gyebangcheon stream. Sampling was conducted twice in July, September, and October 2018. During the July and September surveys, 882 individuals belonging to 13 species from six families were collected. The dominant species was Rhynchocypris kumgangensis and the subdominant species was Zacco koreanus. The total number of B. lenok tsinlingensis collected was 99. The results of the length-weight relationship in the B. lenok tsinlingensis population were analyzed with a regression coefficient b value of 3.1272 and a condition factor (k) value of 0.0006. Therefore, the growth condition of B. lenok tsinlingensis was regarded as fairly good. The QHEI(Qualitative habitat evaluation index) value in the B. lenok tsinlingensis habitat was 119.5(±0.5)-153.5(±0.5), indicating optimal-suboptimal conditions. As a result of the HIS (Habitat suitability index) analysis, it was confirmed that the optimal habitat for B. lenok tsinlingensis was 0.45-0.55m and >1 m in water depth, 0.55-0.65 m s-1 in water velocity, and boulder in the substrate. The ratio of the land use in this study site was analyzed as 66.26-96.31% for forest and grassland areas, 0.00-23.79% for agricultural areas, 0.00-4.19% for urbanized areas, and 3.69-8.87% for others. Correlation analysis of the number of B. lenok tsinlingensis and various factors revealed statistically significant correlations between QHEI and forest and grassland areas, agricultural areas, and urbanized areas.

Food Habits of the Asian Paddle Crab, Charybdis japonica (A. Milne-Edwards) on the Jangbong Tidal Flat, Incheon, Korea (민꽃게, Charybdis japonica (A. Milne-Edwards)의 섭식생태)

  • Seo, In-Soo;Hong, Jae-Sang
    • Korean Journal of Environmental Biology
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    • v.27 no.3
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    • pp.297-305
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    • 2009
  • The Asian paddle crab, Charybdis japonica (A. Milne-Edwards) is an important fishery resource in Korea. Despite its common occurrence and commercial importance, few studies have been carried out on the life cycle and trophic ecology of these populations inhabiting on the tidal flat. Food habits of C. japonica populations were investigated on the Jangbong tidal flat, Incheon, Korea. Monthly samples were taken using a modified otter trawl from November 1999 to January 2001. Based on the examination of stomach contents from 257 individuals, the frequencies of occurrence and the relative volumes of food items were analysed. As a result, the crustaceans (the brachyura, the cirripedia and the harpacticoid copepoda), the echinoderms (the ophiuroidea) and the molluscs (the cephalopoda Loligo beka, the bivalves Bivalvia unid. and Mytilus galloprovincialis) were important food for this species. To examine ontogenetic shifts in diets, individuals of C. japonica were categorized into 2 different size classes (${\leq}60mm$ and ${\geq}61mm$ in carapace width) representing sexually premature and mature stages. The food items of the small crabs (${\leq}60mm$) were dominated by the crustaceans with the harpacticoid copepods constituting 84.6% of total prey number. The next group was the bivalves and the brachyurans. On the other hand, the large crabs (${\geq}61mm$) mainly feed on the bivalvia (Mytilus galloprovincialis and Bivalvia unid.), the brachyurans (Crab unid.) and occasionally the cephalopods and fishes. The difference in proportions of the main prey items among these two size classes was statistically significant. Therefore, the feeding habits of the portunid crab, C. japonica changed with growth.

Ecological Characteristic of Clithon retropictus inhabitating in Yeoncho River in Southern Coastal Area (남해안 연초천에 서식하는 기수갈고둥의 생태적 특성 연구)

  • Lee, Soo-Dong;Kim, Mi-Jeong;Kim, Ji-Suk
    • Korean Journal of Environment and Ecology
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    • v.32 no.6
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    • pp.591-602
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    • 2018
  • Clithon retropictus has been designated as an endangered wildlife Class II due to its high value as a biological indicator species capable of judging environmental quality such as salinity, water flow, and ground conditions. However, basic research on its physiological and ecological characteristics is still lacking. As such, this study intended to examine the impact of environmental conditions such as salinity and soil particle size on the size and density of Clithon retropictus at the Yeoncho river estuary. The investigation of the salinity, which is a key variable that affects the distribution of organisms in the estuary, showed that Clithon retropictus could grow at a salinity ranging from 0#x2030; (freshwater) to 25‰ (brackish water). The coarse gravel (19-75mm) tended to increase nearer the upper stream (under the Yeoncho weir), while the proportion of particles smaller than sand (less than 19mm) increased toward the downstream. The population and the size of the individuals decreased rapidly in the downstream where water stagnated near the Yeoncho weir, and the salt water joined. The results indicated that Clithon retropictus had a high tolerance to salinity, but the adaptability was weaker toward the extremes since the population, and the size tended to decrease as the salinity increased. The correlation analysis revealed that both salinity and soil particle size affected the population and individual size. The correlation between the individual size and salinity was -0.242 (P <0.01), indicating that the size decreased with increasing salinity. The correlation between individual size and coarse gravel having a particle size of 19mm or more was 0.420 (P <0.01), indicating that the size increased with increasing the particle size.

Agronomic Characterization of Sorghum bicolor (L) Moench Germplasm Collected in Gangwon-do for Bio-energy Crop (바이오에너지 자원으로서 강원도 지역에서 수집된 수수의 작물학적 특성 평가)

  • Seong, Eun-Soo;Yoo, Ji-Hye;Lee, Jae-Geun;Hwang, In-Seong;Kim, Nam-Jun;Kim, Myong-Jo;Lee, Ju-Kyung;Ghimire, Bimal Kumar;Lim, Jung-Dae;Yu, Chang-Yeon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.56 no.2
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    • pp.151-158
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    • 2011
  • Forty nine accessions of the Sorghum bicolor (L) Moench collected from different agronomical zone of Gangwondo province, Korea were characterized morphologically on field trial. Plant length, stem diameter, tiller number, ear type, ear length, ear width, leaves length, leaves width and leaf color differed significantly in the cultivated accessions. Out of forty nine test accessions, six accessions (12.2%) showed comparatively taller height, measuring more than 300 cm, while, other ten test accessions (20.4%) were measured from 200~300 cm. There were no significant differences in the diameter of stem in the collected test accessions (2~3 cm). Of the total test accession, seven accessions showed leaf length of over 70 cm, and twenty two test accession revealed leaf length between 60-70 cm. Forty nine of the collected test accessions showed narrow and short leaves with white mid vain. These results provided additional information for the breeding programs and conservation of Sorghum bicolor as a bio-energy crop.

Understanding the Visitation Aspect of Dabbling Ducks (Anas spp.) in the Long Period at Nakdong Estuary, Busan, Republic of Korea (낙동강 하구에서 수면성오리류(Anas spp.)의 장기적 도래 현황)

  • Lee, In-Sup;Hong, Soon-Bok
    • Korean Journal of Environment and Ecology
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    • v.22 no.6
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    • pp.625-631
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    • 2008
  • We used the visitation aspect of dabbling ducks(Anas spp.) to investigate the estuarine ecosystem of Nakdong River and to compare the data collected during two periods: trom May, 1989 to April 1993 and from May, 2002 to April 2006. Over for the 8 years of field surveys, 356,110 individuals from 11 species of dabbling ducks(Anas spp.) were observed in the Nakdong estuary. In the early 1990s, the average number of species gradually increased from October through November, whereas the highest numbers of species were recorded in October during the second survey period. The average number of individuals recorded during the second period was 2-3 times higher than that observed in the 1990s. Statistical analysis using a t-test indicated that the number of individuals showed significant difference at 5% level between November and February in both the early 1990s and the mid-2000s. During the winter, dabbling ducks select both estuarine wetlands as resting sites and rice paddy as feeding sites. More wintering migrants including ducks would visit to Nakdong river if we provide with enough rice paddy near the estuary. The farmland around Nakdong estuary is used by dabbling ducks as a habitat, we hope it must be conserved thoroughly. CRP(crop reserve program), implemented by the local government of Kangseo-gu, Busan City is effective in conserving rice paddy; therefore, the CRP program need to be continuously applied in the Nakdong river basin.

Temporal variation of wintering bird population and environmental factors in Donglim reservoir (동림저수지에서 월동조류 개체군의 시계열적 변화와 환경 요인)

  • Park, Jongchul;Kim, Woo-Yuel
    • Journal of Wetlands Research
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    • v.19 no.2
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    • pp.223-229
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    • 2017
  • This study analyzed the relationship between environmental factors and the temporal changes of the bird populations during the every January from 1999 to 2016 (18 years) in Donglim reservoir. 'Winter Waterbird Census of Korea' data by National Institute of Biological Resources were used for the bird population data, and principal component analysis and cluster analysis were used to analyze the changes of annual population. The average temperature of January and the average storage rate of the reservoir were used as environmental factors. According to the results of the study, the population changes in the study area can be explained by the increase and decrease of two water bird groups and a mountain bird group. The average temperature of the years when the population of water bird groups increased was more than $1.4^{\circ}C$ in comparison with the year when mountain birds increased. On the other hand, the influence of the water content was not clear. The visiting of Baikal Teal affected by temperature was a factor affecting the other group of water bird and a mountain bird group. The results of this study suggest that the temperature change affects the global spatial distribution of birds and the migration of large population species such as Baikal Teal affects species composition and populations of birds at local scale. Therefore, understanding of environmental changes at large scale and spatial distribution of species and flock contributes to understand the temporal variation of the bird population at regional or local scales.