• Title/Summary/Keyword: 이물질

Search Result 534, Processing Time 0.024 seconds

공유결합으로 고정화된 urokinase 칼럼의 스케일업과 solid-phase refolding에 의한 반복 사용

  • Seo, Chang-U;An, Sang-Jeom;Lee, Eun-Gyu
    • 한국생물공학회:학술대회논문집
    • /
    • 2001.11a
    • /
    • pp.85-88
    • /
    • 2001
  • We scaled up a covalent immobilization system of urokinase to the activated Sepharose and used it repeatedly to cleave a fusion protein consisting of human growth hormone and GST fragment. After scale up from 6 ml to 250 ml, the column system still demonstrated basically the same performance in terms of urokinase immobilization and fusion protein cleavage. When the column was washed with 6M guanidine HCl after the cleavage reaction. the immobilized urokinase showed no activity probably because it was fully unfolded. However. as the denaturant was gradually removed from the column the immobilized urokinase fully regained its bioactivity. which indicated it was properly refolded into its native conformation as covalently attached to the solid matrix. After 20 cycles of this 'solid-phase unfolding/refolding', the immobilized urokinase maintained approx. 80% of the initial bioactivity. This method provides an efficient protocol to apply the solid-phase refolding technique to improve the longevity of immobilized enzyme columns.

  • PDF

Transobturator Tape Under the Ultrasonographic Guidance -A Case Report- (초음파 유도하 경폐쇄공 띠의 제거 -증례 보고-)

  • Min, Kyoung-Dae;Hwang, Seok-Ha;Kang, Seung-Gu;Lee, Byung-Ill
    • The Journal of Korean Orthopaedic Ultrasound Society
    • /
    • v.5 no.2
    • /
    • pp.118-122
    • /
    • 2012
  • Taking history and ultrasonography is important to detect foreign body which might be located in the soft tissue. We encountered a case of soft tissue foreign body (transobturator tape) in a patient who had been treated with the impression of adductor myositis resulting from vagina fistula. The foreign body was found through ultrasonographic examination and the patient's symptom was relieved after removal of the foreign body from the ultrasonography-guided operation. Clinicians should keep in mind the fact that foreign bodies may exist when the inflammation persists without appreciable cause and ultrasonography can be useful tool in case of small fibrous band not detected with magnetic resonance imaging due to inflammatory change of the surrounding tissues.

  • PDF

A Foreign Body Found in the Pulmonary Artery of a Traffic Accident Victim with a Chest Injury and Near-amputation of the Upper Extremity - A case report - (흉부 손상과 함께 우상지에 절단에 가까운 열창을 입은 교통 사고 환자에서 발견된 폐동맥 내 이물질 - 1예 보고 -)

  • Choi, Goang-Min;Kim, Heung-Cheol;Cho, Kwang-Yun;Kim, Hyung-Soo
    • Journal of Chest Surgery
    • /
    • v.41 no.4
    • /
    • pp.536-539
    • /
    • 2008
  • We present a case with a foreign body in the left pulmonary artery, found in a traffic accident victim. A 52-year-old woman sitting in the passenger side of a car had massive bleeding and near complete amputation of her right forearm in addition to multiple rib fractures and a hemopneumothorax. At arrival to the emergency room, the patient had signs of shock; she was anemic, drowsy and hypotensive. A large volume of blood and crystalloid fluids were administered via the left subclavian vein with a rapid infusion device (Level $1^{(R)}$). As the lung contusion improved, a foreign body was noticed in the left lung field on plain x-rays. Pulmonary angiography was performed and revealed a 15 cm foreign body in the left basal segment of the common pulmonary artery. The foreign body was successfully retrieved using vascular forceps via the percutaneous femoral vein approach.

Evaluation of Impurity Content Criteria of Recycled Aggregate for Lean Concrete Base (빈배합 콘크리트 기층용 순환골재의 이물질 품질기준 적정성 연구)

  • Kim, Nam-Ho;Yang, Seung-Cheol
    • International Journal of Highway Engineering
    • /
    • v.14 no.3
    • /
    • pp.69-76
    • /
    • 2012
  • A recent shortage in Korean aggregate market leads a social demand to utilize recycled aggregate to more advanced level, such as the use in concrete structures or paving materials for surface and base layers. Government announced a recycled aggregate guideline in 2009 to provide an institutional framework for recycled aggregate in such an up-scaled use. The use of recycled aggregate in such use; however, is very minimal. This paper evaluates the validity of the impurity content criteria of recycled aggregate for lean concrete base through a series of material tests. The analysis results shows that reclaimed asphalt pavement (RAP) in recycled aggregate not only influence a strength lean concrete adversely, but also influence negatively on an absorption and abrasion characteristics of aggregate system significantly that made those two indices lower. Since absorption and abrasion characteristics are very important indices for recycled aggregate quality, RAP in recycled aggregate could significantly mislead the recycled aggregate qualification. This paper provides a suggestion to resolve these problems.

Experiment on Automatic Detection of Airport Debris (FOD) using EO/IR Cameras and Radar (EO/IR 카메라 및 레이더를 이용한 공항 이물질(FOD) 자동탐지 실험)

  • Hong, Jae-Beom;Kang, Min-Soo;Kim, Yun-Seob;Kim, Min-Soo;Hong, Gyo-Young
    • Journal of Advanced Navigation Technology
    • /
    • v.22 no.6
    • /
    • pp.522-529
    • /
    • 2018
  • FOD refers to various metals and non-metallic foreign substances that pose a risk to aircraft. FODs occur in all areas and time zones, including runways, taxiways, and maintenance facilities, and pose a fatal hazard to aircraft safety during aircraft movements and take-off and landing. Rapid and effective detection and removal of FODs in the runway is required. As part of recent developments in aviation safety technologies, automatic detection of debris in runways in airports is under way. In this paper, we conducted an automated detection test using the EO/IR camera and radar at the Taean campus of Hansu University to confirm normal detection during the day and night.

A study on the Improvement of the Food Waste Discharge System through the Classification on Foreign Substances (이물질 구별을 통한 음식물쓰레기 배출시스템 개선에 관한 연구)

  • Kim, Yongil;Kim, Seungcheon
    • The Journal of the Institute of Internet, Broadcasting and Communication
    • /
    • v.22 no.6
    • /
    • pp.51-56
    • /
    • 2022
  • With the development of industrialization, the amount of food and waste is rapidly increasing. Accordingly, the government is aware of the seriousness and is making efforts in various ways to reduce it. As a part of that, the volume-based food system was introduced, and although there were several trials and errors at the beginning of the introduction, it shows a reduction effect of 20 to 30%. These results suggest that the volume-based food system is being established. However, the waste is caused by foreign substances in the process of recycling resources by collecting them from the 1st collection to the 2nd collection process. Therefore, in this study, to solve these problems fundamentally, artificial intelligence is applied to classify foreign substances and improve them. Due to the nature of food waste, there is a limit to obtaining many images, so we compare several models based on CNNs and classify them as abnormal data, that is, CNN-based models are trained on various types of foreign substances, and then models with high accuracy are selected. We intend to prepare improvement measures for maintenance, such as manpower input to protect equipment and classify foreign substances by applying it.

Clinical analysis of acute drug intoxication and foreign body ingestion in Wonju: comparison between the 1990s and the 2000s (1990년대와 2000년대의 원주시 소아에서 발생한 급성약물중독과 이물질섭취 환아의 비교분석)

  • Eum, Joo Pil;Suh, Jin Suk;Kim, Hwang Min
    • Clinical and Experimental Pediatrics
    • /
    • v.50 no.2
    • /
    • pp.138-142
    • /
    • 2007
  • Purpose : The purpose of this study is to find out the changing pattern of drug or foreign body ingestion between 1990s and 2000s in Wonju Christian Hospital, which is located in one of the most rapidly urbanizing cities in Korea. Methods : We retrospectively reviewed the medical records of patients with acute drug intoxication and foreign body ingestion from January 1991 to December 1994 and from January 2001 to December 2004. We performed clinical analysis and compared the results of the 1990s and 2000s. Results : There were 43 cases of acute drug intoxication in the 1990s, and 35 cases in the 2000s. Foreign body ingestion was 47 cases in the 1990s and 22 cases in the 2000s. The ingested foreign bodies were chiefly coins (46.8 percent), Weiqi chips (14.9 percent), plastic toys (6.4 percent) and others (31.9 percent) in the 1990s. In the 2000s, others (58.2 percent) were the most commonly ingested foreign body with mercury batteries (23.8 percent), nails (9 percent), pins (9 percent) following. The most common cause of drug intoxication was medical drugs (37.2 percent) others (23.2 percent), agricultural agents (13.9 percent), carbon monoxide (11.6 percent), rodenticide (9.3 percent), insecticide (9.3 percent) in the 1990s. In the 2000s the most common causes were others (34.3 percent), medical drug (31.4 percent), agriculture agents (20 percent), detergent (11.4 percent), insecticide (2.9 percent) and carbon monoxide (2.9 percent). The highest incidence age group was the 1-3 year old group, both in acute drug intoxication and foreign body ingestion. There were no changes in the incidence age group between the 1990s and the 2000s. Conclusion : There were some significant changes in the cause of acute drug intoxication and foreign body ingestion between the 1990s and 2000s. New education programs for preventing acute drug intoxication and foreign body ingestion are important and necessary.

Cause Investigation and Verification of Fire Accidents for Road and Street Lighting (가로등 화재사고의 원인규명과 검증)

  • Shong, Kil-Mok;Han, Woon-Ki;Kim, Jong-Min;Kim, Young-Seok;Kim, Myoung-Soo;Kim, Hyeok-Soo
    • Journal of Korean Institute of Fire Investigation
    • /
    • v.11 no.1
    • /
    • pp.31-36
    • /
    • 2008
  • This paper carried out a variety of analysis for a finding out the causes of fire on the street lamp. The glass bulb of 250W metal-halide(MH) lamps attached with the impurities rose the temperature by at $410.4^{\circ}$ in normal condition. Therefore, a lamp apparatus is needed the improvement for ventilation and the protection against dust. The heat of MH lamps is diffused through out the luminous tube, a glass bulb, and apparatus by degrees. The accident cause of the lamp is presumed that the accumulated heat by deposits of the inner part. It will be recommended the improvement of the structure that aren't the external impurity and accumulated heat in the MH lamps set.

  • PDF