• Korean journal of applied entomology
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• v.46 no.3
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• pp.385-392
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• 2007

This research was carried out to develop economical artificial diets of the greater wax moth, Galleria mellonella (L.). Diets were mixed with malt or sugar instead of whole honey for cheaper ones. fecundity, developmental period, pupation rate, emergence rate and adult longevity of G. mellonella depending on diet were examined. The head width and length of larvae were not significantly different among diets tested. However, the weight of larva was the heaviest, 255.5 mg, at 1/2 honey + 1/2 malt diet and lightest, 144.3 mg, at sugar diet. The weight of pupa was also the heaviest at 1/2 honey + 1/2 malt representing 196.7 mg. Larval period was the shortest at honey diet as 30.9 days but the longest at sugar diet as 36.5 days. Pupation and emergence rates were over 79.3% at all diets. The longevity of mated females was ranged from 6.3 to 8.0 days and those of mated males ranged from 7.9 to 11.2 days. The highest number of eggs, 1269, was obtained at sugar diet. Artificial diets replaced by rice bran, malt, and sucrose reduced costs compared with wheat bran plus honey diet. The cost of rice bran was only 10% of wheat bran. Honey was much more expensive than malt and sugar, that is 6 and 13 times, respectively.

• Korean journal of applied entomology
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• v.37 no.2
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• pp.137-142
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• 1998

The regional distribution and some ecological characteristics of Chinese white-wax scale, Ericerus pela Chavannes, were investigated from 1996 to 1997. It was found that 10.8% of the stems of the privet, Ligustrum obtusifolium infested with the scale in Chtingpyijng, whereas only 1.1% of them infested in Y6ngwol and Pyongtaek. The average survival rate of the female adult marked 85.3% after it hibernated on the privet, Ligustrum obtusifolium. A female laid 7,783.5 eggs in average and 36.7% of females fell on the range of 7,000-10,000 eggs. It sized 0.40 mm in length and 0.21 mm in width. The hatchability was highest at 27$^{\circ}$C with 66.8% and it seemed the optimum temperature for incubation. The pupation rate was lower than 50.0% at the above experimental temperatures and the emergence rate marked 67.3% at 25$^{\circ}$C. When the egg was preserved at the various low temperatures, it was found that the egg could be preserved at lS$^{\circ}$C for 50 days in maximum.

• Korean journal of applied entomology
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• v.51 no.4
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• pp.469-477
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• 2012

Ostrinia larvae feed the pods and stem of red bean and seriously damage the bean production from farmers. In this study we investigated biological and developmental characteristics including field collection, host feeding preference, artificial rearing diet, morphological and molecular taxonomical identification, and pheromone analysis for an Ostrinia sp. in Korea. The male adults have massive tibia in the middle legs and 3-lobed uncus in the genitalia. The partial nucleotide sequences of mitochondrial cytochrome oxidase I (COI) and II (COII) were not corresponded to those DNA sequences from other Ostrinia species reported previously in Japan and China. Host plants for this species are also different from the previous species reported. In the gas chromatography (GC) analyses, (Z)-9-tetradecenyl acetate was not detected from the pheromone gland of our species while the component as a sex pheromone was found in O. zaguliaevi and O. zealis, With taken results, we conclude this Ostrinia species in Korea is Ostrinia scapulalis or closely related species. When larvae collected in a fall were incubated in the outdoor condition, they emerged to adult between June and July in the next year. The result indicates that the winter diapause could be started in late larval stage. In addition, we developed a semi-synthetic artificial diet adopted for mass rearing of the O. scapulalis in laboratory.

• Korean Journal of Environmental Biology
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• v.28 no.2
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• pp.64-68
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• 2010

The mosquito, Aedes albopictus is important vector of humen phatogen such as dengue fever. And Aedes albopictus has widly distribution in oriental regions. This study was conducted to investigate the biological effects of cooper on the development of the Asian tiger mosquitoes, Aedes albopictus (Skuse). Aedes albopictus was affected with pupation ratios, mortalities, and wing lengths of the 3th and 4th instars by exposure to different concentration $CuCl_2$ (0.0, 2.5, 25.0 and 50.0 ppm) for 24 h and 48 h depending on dose and exposure period. The lowest $LC_{50}$ (median lethal concentration) value of $CuCl_2$ was 18.1 ppm at the 3rd instars for 48 h exposure. The pupation ratio of the larvae exposed at 50.0 ppm for 48 h was 14% which was much lower than those exposed to other treatment groups Changes of adult wing length showed significant decreased by Cu treatment. In conclusion, the most prominent effects of high concentration and longer exposure period were to reduce in the survival and pupation rates of larvae and wing length of Ae. albopictus.

• Parasites, Hosts and Diseases
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• v.34 no.1
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• pp.21-26
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• 1996

An experimental study was carried out to observe the effects of radiation on the infectivity of metacercariae of Gymnophalloines seoi to C3H mice. Oysters, the second intermediate host, were collected from an endemic area, and non-irradiated control, metacercaria-irradiation, and oyster-irradiation groups were prepared . One hundred metacercariae were infected orally to each mouse, and worm recovery rate was compared by groups at 7th day post-infection. In the metacercaria-irradiation group, the worm recovery rate was significantly reduced at radiation doses higher than 200 Gy, and the number of intrauterine eggs significantly reduced at doses over 50 Gy. In the oyster- irradiation group, 50 Gy significantly reduced both the worm recovery rate and number of uterine eggs. In the two groups, no worm was recovered at 1,000 Gy irradiation. Conclusively, irradiation of oysters with 200-1,000 Gy was effective to control infectivity of metacercariae to C3H mice, and could be adopted as a control measure for gymno-phalloidiasis.

• Korean journal of applied entomology
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• v.49 no.4
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• pp.351-355
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• 2010

This study was conducted to determine the relationship of larval density of tobacco cutworm, Spodoptera litura (Fabricius) to damage in greenhouse sweet pepper. Laboratory experiments, cage experiments by artificial release and field investigation were carried out in 2008. The leaf consumption rate increased greatly with larval development. The damaged leaves had several round or oval shape holes on the surface or lost certain parts of them, and the fruit damaged had a conspicuous hole on the surface or scar marks around the calyx. In the field investigation, fruit damage was highly correlated with larval densities and reached 3.5% damage at maximum. Cage experiments showed that numbers of non-marketable fruit increased as increasing larval densities released. The larval density at two weeks before harvest had a high relationship with the percentage of damaged-fruit at harvest. Corresponding larval density caused 1, 3, 5% of damaged-fruit was 0.2, 0.5, 0.8 larvae per plant, respectively.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.9 no.1
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• pp.35-42
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• 1976

Larval morphogenesis and infection rates of Cercaria pectinata Huet were investigated in regards to histological changes and mortality of the hard clam, Meretrix lusoria $(R\ddot{o}ding)$, in Buan located on the Western Coast of Korea. The trichocerous cercariae, Cercaria pectinata, and its sporocysts parasitize mainly in the tissues of goand, digestive gland and gills of the hard clams. One branched sporocyst grows into a great number of cylindrical sporocysts, and a heavily infected clam possesses 2,000 to 5,000 cylindrical spo ro cysts. Each of them produces approximately 30 to 70 germinal cells and cercariae. Of 2,639 clams examined 347 individuals $(13.2\%)$ were found to be infected. The infection rates showed seasonal variations, the rate being highest $(29.6\%)$ in July, and lowest $(2.0\%)$ in March. Whereas, the infection rates did not change significantly with the changes of size class of the shell. No infection was observed in Meretrix petachialis (Lamarck) collected from Myeong-ji, Kimhae Gun located near Busan. In the iefected clams, the gametogenesis (oogenesis and apermatogenesis) did not undergo completely, therefore the heavily infected clam seemed to be castrated. Also the tissues of the digestive gland and gills were compressed by the multiplication of the parasites. When they were kept in aquariums in the laboratory, high mortality has occurred in the infected clams. All of the 60 infected clams died within 35 days.

• Korean journal of applied entomology
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• v.29 no.4
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• pp.277-285
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• 1990

Laboratory and field studies were conducted to investigate the initiation and termination factors for pupal diapause in the Oriental tabacco budworm, Heliothis assulta Guenee. Diapause induction was dependent of photoperiod and temperature experienced by 4th and 5th instar larve. One hundred percent of pupal diapause was obtained when larvae were grown at 10L/14D or shorter and $20^{\circ}C$. But at $25^{\circ}C$ the maximum rate of diapause was only about 85% at 8L/16D, the shortest photoperiod tested in this experiment. Adults, eggs or pupae did not respond to diapause-inducing environmental conditions. The critical photoperiod was 12-12.5 hr at $25^{\circ}C$and 14-14.5 hr at $20^{\circ}C$. In the field-collected sample 100% of diapuse rate was observed on Sept. 20 and thereafter in Suwon and middle part of Korea. Cold temperature treatment was not necessary in the termination of pupal diapause, but accelerated adult development.

• Korean journal of applied entomology
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• v.41 no.1
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• pp.43-47
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• 2002

This study was carried out to investigate the ecological characteristics of Protaetia orientalis submarmorea (Burmeister). The emergence period of adults is appeared from April to October, and most abundant in July∼August, once a year in the field. Hibernation took place mostly in larval stage, but some in adult stage, and were generally found in the decayed rice straws and in the soil covered with compost or similar materials with average depth of 17.6 cm The developmental characteristics in the insectary (28$^{\circ}C$, 70% RH, 16L: 8D) was investigated: the average number of eggs laid per female is 82.8, egg period 8.4 days, 1st instars of larva 10.6, 2nd 18.7, 3rd 38.1, and pupal period 35.5 days. Longevity of the adult was 135.2 days In the female and 121. 7days in the male.

• Asian Journal of Turfgrass Science
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• v.12 no.2
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• pp.127-140
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• 1998

곤충병원성 선충의 이용 효율을 높이기 위하여 곤충병원성 선충 상호간 또는 선충과 곤충병원성 곰팡이와의 상호관계를 알아보았다. Steinernema glaseri는 기주의 무게에 따라 성충수에서는 차이가 없었으나 침입태 유충수에서는 차이가 있었다. 선충의 접종 농도에 따른 침입 발육된 성충수는 기주당 20마리 이하의 접종에서는 차이를 보였으나 40마리 이상 1,000마리 접종구에서는 차이가 없었고, 증식된 유충수도 기주당 1,000마리 접종구에서 현저히 적은 것을 제외하고는 차이가 없었다. 동일 기주에서의 선충 상호간 관계에서는 steinernematid 선충에 의한 기주 치사율이 heterorhabditid 선충에 의한 것보다 높았다. 즉, 서로 다른 종의 곤충병원성 선충을 동일 기주에 접종하였을 때, S. carpocapsae 포천에 의한 기주 치사율은 $76.2\pm$4.8%였고 Heterorhabditis bacteriophora 함양에 의한 치사율은 $23.8\pm$4.8%였다. 또한 S. carpocapsae 포천에 의한 기주치사율이 각각 $90.5\pm$4.8%와 $80.9\pm$4.8%, H. bacteriophora NC 1에 의한 것은 9.5$\pm$4.8%였다. S. glaseri NC와 H. bacteriophora 함양 및 H. bacteriophora NC 1을 동시 접종하였을 때는 S. glaseri NC에 의한 것이 각각 $61.9\pm$9.65%와 $80.9\pm$4.8%, H. bacteriophora 함양에 의한 것이 $38.1\pm$9.5%, H. bacteriophora NC 1에 의한 것이 $19.1\pm$4.8%였다. 그러나 두 선충의 동시 감염은 관찰되지 않았다. 그리고 S. carpocapsae All과 곤충병원성 곰팡이인 Beauveria brongniartii를 동시 또는 곰팡이를 먼저 처리했을 때는 곰팡이 12시간 전 처리부터 선충과 곰팡이의 동시 감염이 관찰되었고, H. bacteriophora NC 1는 곰팡이 6시간 전 처리부터 동시 감염이 관찰되었다. 선충에 의한 감염과, 곰팡이에 의한 감염, 선충과 곰팡이 동시 감염은 곰팡이 48시간 전 처리부터 관찰되었다. 그러나 유충 증식수는 선충 단독 감염보다 동시 감염충에서 현저히 떨어졌다.