Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
권호 표지
DOI QR코드

DOI QR Code

Development of Economical Artificial Diets for Greater Wax Moth, Galleria mellonella (L.)

꿀벌부채명나방[Galleria mellonella (L.)] 사육을 위한 경제적 인공사료 개발

  • Lee, Seung-Wook (Sesil Corporation) ;
  • Lee, Dong-Woon (Department of Applied Biology, Institute of Agricultural Sciences, Sangju National University) ;
  • Choo, Ho-Yul (Department of Applied Biology and Environmental Sciences, Institute of Agricultural and Life Sciences, Gyeongsang National University)
  • 이승욱 (주식회사 세실) ;
  • 이동운 (상주대학교 생물응용학과) ;
  • 추호렬 (경상대학교 응용생물환경학과 농업생명과학연구원)
  • Published : 2007.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

This research was carried out to develop economical artificial diets of the greater wax moth, Galleria mellonella (L.). Diets were mixed with malt or sugar instead of whole honey for cheaper ones. fecundity, developmental period, pupation rate, emergence rate and adult longevity of G. mellonella depending on diet were examined. The head width and length of larvae were not significantly different among diets tested. However, the weight of larva was the heaviest, 255.5 mg, at 1/2 honey + 1/2 malt diet and lightest, 144.3 mg, at sugar diet. The weight of pupa was also the heaviest at 1/2 honey + 1/2 malt representing 196.7 mg. Larval period was the shortest at honey diet as 30.9 days but the longest at sugar diet as 36.5 days. Pupation and emergence rates were over 79.3% at all diets. The longevity of mated females was ranged from 6.3 to 8.0 days and those of mated males ranged from 7.9 to 11.2 days. The highest number of eggs, 1269, was obtained at sugar diet. Artificial diets replaced by rice bran, malt, and sucrose reduced costs compared with wheat bran plus honey diet. The cost of rice bran was only 10% of wheat bran. Honey was much more expensive than malt and sugar, that is 6 and 13 times, respectively.

본 연구는 꿀벌부채명나방의 경제적 사육을 위한 인공사료 개발을 위하여 수행하였다. 가격이 비싼 벌꿀을 대체하여 물엿과 설탕을 이용한 사료별 꿀벌부채명나방의 발육과 발육기간 및 산란수, 성충 수명 등을 조사하였다. 당 종류별 유충의 두폭과 체장은 비슷하였으나 체중은 1/2벌꿀 + 1/2물엿 처리가 255.5 mg으로 가장 무거웠고, 설탕은 144.3 mg으로 가장 가벼웠다. 꿀벌부채명나방 번데기의 체중도 1/2벌꿀+1/2물엿이 196.7 mg으로 가장 무거웠다. 당 종류별 꿀벌부채명나방의 유충 발육기간은 벌꿀이 포함된 사료가 30.9일로 가장 짧았으며 설탕은 36.5일로 가장 길었다. 꿀벌부채명나방의 용화율과 우화율은 79.3% 이상이었다. 모든 사료에서 꿀벌부채명나방의 교미 성충의 수명은 암컷이 $6.3{\sim}8.0$일이었고, 수컷은 $7.9{\sim}11.2$일이었다. 사료별 꿀벌부채명나방 암컷 성충의 산란수는 설탕물 첨가 사료에서 1,269개로 가장 많았다. 당 종류별 인공사료 조성에 대한 비용은 밀기울이 쌀겨보다 10배 정도 많았으며, 벌꿀은 물엿과 설탕의 6배와 13배였다.

Keywords

References

  1. Arnett, R.H. 1985. Galleria mellonella: In American Insect. p. 570. Published by van Nostrand Reinhold Company Inc. New York, USA
  2. Beck, S.D. and J.F. Stauffer. 1950. An aseptic method for rearing European com borer larvae. J. Econ. Entomol. 43: 4-6 https://doi.org/10.1093/jee/43.1.4
  3. Bedding, R.A. and R.J. Akhurst. 1975. A simple technique for the detection of insect parastic rhabditid nematodes in soil. Nematologica 21: 109-110 https://doi.org/10.1163/187529275X00419
  4. Charriere, J.D. and I. Anton. 1997. Protection of honey combs from moth damage. Swiss Bee Research Center. Communication Nr. 24
  5. Cho, LH. 1996. Practice and application of SAS. 665pp. Sungandang Pub. Co. Seoul
  6. Fan, X. and W.M. Hominick. 1991. Efficacy of the Galleria (wax moth) baiting technique for recovering infective stages of entomopathogenic rhabditids (Steinemematidae and Heterorhabditid) from sand and soil. Revue Nematol, 14: 381-387
  7. Hwang, S.J., Y.I. Mah, M.Y. Lee and H.S. Bang. 1998. The development of great wax moth, Galleria mellonella L., on the various rearing temperatures in the laboratory condition. Kor. J. Apicul. 13: 73-78
  8. Kaya, H.K. and R. Gaugler. 1990. Entomopathogenic nematodes in biological control. CRC Press. Boca Raton
  9. Kim. C.H. 1993. Method of insect rearing. Gyeongsang National University Press. Jinju. 313pp
  10. Ladd, T.L.Jr. 1986. Influence of sugars on the feeding response of Japanese beetles (Coleoptera: Scarabaeidae). J. Econ. Entomol. 79: 668-671 https://doi.org/10.1093/jee/79.3.668
  11. Lee, H.I., J.S. Ryou and K.R. Lee. 1994. Trehalose, cAMP concentration and juvenile hormone esterase activity during the metamorphosis in the great wax moth, Galleria mellonella. Kor. J. Entomol. 24: 49-56
  12. Lee, M.L., M.Y. Choi and K.S. Choi. 1991. Control effects of Galleria mellonella in honeybee combs with aluminiumphosphide, its residues and safety on honeybees. Kor. J. Apicul. 6: 12-15
  13. MacMacters, M.M., J.J.C. Hinton and D. Bradbury. 1971. Microscopic structure and composition of the wheat kernel. In Wheat chemistry and technology ed. by Y. Pomeranz, 3: 51-113. St. paul. Minn: Am. Assoc. Cereal Chemists. 821pp
  14. Nielsen, R.A. and C.D. Brister. 1979. The great wax moth: adult behavior. Ann. Entomol. Soc. Am. 70: 101-103
  15. Oh, H.W., M.Y. Lee and Y.D. Chang. 1995. Development periods and damage patterns of combs by the great wax moth, Galleria mellonella. Kor. J. Apicul. 10: 5-10
  16. Patrick, D.C., F. Merlevede and L. Tirry. 1998. Unnatural prey and artificial diets for rearing Podisus maculiventris (Heteroptera: Pentatomidae). Biol. Contr. 12: 137-142 https://doi.org/10.1006/bcon.1998.0611
  17. Poinar, G.O.Jr. 1975. Entomogenous nematodes: a manual and host list of insect-nematode association. E.J. Brill. Leiden. The Netherlands. p. 27
  18. Seol, K.Y., H.S. Kim and B.Y. Choi. 2005. Insect rearing methods. Hanrimwon. Seoul. 294pp
  19. Sin, W.K., H.G. Kim and K.Y. Cho. 1997. Effect of the composition of artificial diets on the growth and development of a beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidae). Kor. J. Entomol. 27: 181-184
  20. Woodring, J.L. and H.K Kaya. 1988. Steinernematidae and Heterorhabditidae nematodes: a handbook of techniques. Southern Coop. Ser. Bull. 331, Alkansas Agri. Exp. Stn. Fayetteville, AR. 29pp

Cited by

  1. Cost-Effective Culturing ofGalleria mellonellaandTenebrioMolitor and Entomopathogenic Nematode Production in Various Hosts vol.23, pp.2, 2015, https://doi.org/10.4001/003.023.0232
  2. The Role of Entomopathogenic Nematodes as Biological Control Agents of Insect Pests, with Emphasis on the History of Their Mass Culturing andin vivoProduction vol.22, pp.2, 2014, https://doi.org/10.4001/003.022.0222
  3. Effect of Harvest Time and Infective Juvenile Size of the Entomopathogenic Nematode, Steinernema arenarium, on Pathogenicity, Development, and Propagation vol.51, pp.1, 2012, https://doi.org/10.5656/KSAE.2012.01.1.058
  4. vol.6, pp.7, 2018, https://doi.org/10.1128/genomeA.00044-18