• Korean Journal of Optics and Photonics
• /
• v.19 no.1
• /
• pp.73-79
• /
• 2008

We propose a novel metal-waveguide structure for sustaining long-range surface-plasmon-polaritons (LRSPP). The LRSPP waveguides are composed basically of two asymmetric metal layers: a very thin, finite-width metal strip on top of a metal slab with a dielectric gap in between them. Mode cut-off of LRSPPs excited on the double-metal waveguides is characterized by consistently investigating their dispersion relations and mode profiles. We also confirm experimentally the existence of low-loss, well-confined LRSPP modes by measuring far-field outputs emerging from an edge of the asymmetric double-metal waveguides. In the experiment, we have fabricated several types of SPP waveguide devices including straight lines, S-bend, and Y-branch consisting of gold strips (20 nm-thick, $5{\mu}m$-wide). Overall propagation loss of the proposed double-metal waveguides is quite comparable to that of single metal-strip waveguides, in addition the mode sizes can be tuned by increasing the core-insulator gap between the metal layers to get a higher coupling efficiency with a single-mode fiber in telecom wavelength. The proposed LRSPP waveguides may open up realization of SPP-waveguide sensors or nonlinear SPP-devices by replacing the core-insulator with a bio-fluid or a nonlinear medium.

• Korean Journal of Plant Taxonomy
• /
• v.37 no.3
• /
• pp.225-237
• /
• 2007

In order to estimate the genetic relationships among four taxa of Korean Symplocaceae and their regional populations, RAPDs analysis was performed. The length of the amplified DNA fragments ranged from 150 to 1,900 bp. Ninety-two RAPD bands were scored for 11 primers and the genetic distance was calculated with by Nei-Li's genetic dissimilarity. Deciduous and evergreen groups were clearly separated in the UPGMA analysis. Symplocos coreana was clustered as a distinct group, and S. sawafutagi and S. tanakana were also clustered at specific level, respectively. The RAPD data was useful to cognize the genetic variation and to discuss the relationships among Korean Symplocaceae

• Korean Journal of Agricultural Science
• /
• v.28 no.1
• /
• pp.8-17
• /
• 2001

This study was carried out to obtain a basic intonation for the development of a new com hybird with tillers. Materials used in this study were 20 lines having three to four tillers per plant including the PI213749 U.S. line with non-tillers. These 20 lines were compared for the botanical characteristics and genetic distances were measured using RAPD analysis. Flowering date of the K15 was very earlier, while the K07 was very late in flowering date. Stem height and ear height were similar except for K04 and the K15. K06, K13 and K19 lines were appeared to be resistant to lodging due to decreased ear height. Number of tillers per plant of lines used were shown three to five on average. K09 showed the highest kernel yield, while the K08 was low. Among characteristics measured tiller per plant and flowering dates, and silking dates showed a positive correlation, while 100 kernels weight, flowering date and ear height were shown a negative with tillers per plant. A total of 17 bands by RAPD analysis using four per primer were appeared and these lines were classified into three groups, especially the third group could be classified into of four sub-groups.

• Korean Journal of Ichthyology
• /
• v.20 no.3
• /
• pp.156-162
• /
• 2008

Five microsatellite DNA markers were used to investigate genetic diversity and population structure of olive flounder Paralichthys olivaceus collected from four locations (YD, SC, GJ, WD) where hatchery-based seeds of the flounder have been released. The average of observed (Ho) and expected heterozygosity (He) ranged from 0.833 to 0.871, and from 0.842 to 0.876, respectively. The average number of alleles per locus ranged from 12.4 to 17.8. The proportion of stocked flounder ranged from 20.0% to 95.8% for wild-caught populations with a decreasing tendency of alleles per locus following a higher proportion of stocked flounder. There is need to implement a more careful stock-enhancement program of hatchery-based seeds and to monitor its genetic effects on wild populations to ensure conservation of natural flounder resources.

• Animal Systematics, Evolution and Diversity
• /
• v.17 no.1
• /
• pp.49-57
• /
• 2001

The partial sequences of mtDNA cytochrome b gene in two subspecies of striped field mouse(Apodemus agrarius coreae and A. agrarius manchuricus) from Korea and northeast China were analyzed to determine the degree of genetic diversity in ech subspecies and to confirm their subspecific difference. In 18 specimens of A. agrarius coreae, ten haplotypes were resulted, and in two specimens of A. agrarius manchuricus. one haplotype was revealed. Tamura-Nei nucleotide distance among ten haplotypes in subspecies coreae ranged 0.36 to 1.86%. and nucleotide distance between two subspecies (coreae and manchuricus) was 0.37 to 1.47%: maximum infrasubspecific divergence in coreae was greater than maximum intersubspecific difference between two subspecies. Moreover, no major subgroup was resulted when 11 haplotypes in two subspecies were compared. Our sequence result was not cancordant with the morphological data studied so far, and it is concluded that cytochrome b gene sequence is not a good genetic marker to distinguish two subspecies of A. agrarius. In futurem, mtDNA control region analyses seemded to be necessary to reveal genetic relationship between these two subspecies of A. agrarius.

• Korean journal of applied entomology
• /
• v.53 no.4
• /
• pp.355-365
• /
• 2014

It has been unclear whether the diamondback moth, Plutella xylostella can overwinter in Korean field conditions. This study determined overwintering conditions of P. xylostella by conducting field exposure tests based on its cold tolerance and monitoring overwintering populations by direct examination of overwintering larval habitats and capturing adults with sex pheromone traps. In addition, the overwintering populations were analyzed using polymorphic genetic markers to trace their sources. When all immature stages of P. xylostella were exposed to $-5^{\circ}C$, which was the temperature much above their supercooling points, they significantly suffered with direct cold injuries, where larval stage was most tolerant to the cold injury. However, the exposure to $5^{\circ}C$ for a long period (4 weeks) did not give any significant cold injury to nonfeeding stages, while this treatment gave lethality to larval stage without diet. When all developmental stages of P. xylostella were exposed to open field conditions during winter, they exhibited significant decreases of survival rates. However, some protected and indoor conditions reduced the cold injuries and the diet provision significantly increased larval survival rates. Adult monitoring with sex pheromone during winter period indicated that the first captures were observed at similar periods at different locations (${\approx}260$ Km apart). The overwintering adults were captured until early April. Genetic variation of these overwintering populations was analyzed with polymorphic molecular markers, indicating significant genetic divergences among the overwintering populations. This study indicates that P. xylostella can overwinter in southern Korean fields or some protected greenhouses with host plants.

• Korean Journal of Ecology and Environment
• /
• v.52 no.3
• /
• pp.221-230
• /
• 2019

Barbel steed (Hemibarbus labeo) is a small freshwater fish species as semi-bottom dwellers distributed in eastern Asia. We carried out characterization of the cytochrome c oxidase subunit I (COI) gene from the mitochondrial DNA of H. labeo in the Sumjin River to identify the phylogenetic location of H. labeo in the genus Hemibarbus and Cyprinidae. Multiple alignment of the 577 bp COI sequence revealed high sequence homology (99~100%) between Seomjin River H. labeo. The nucleotide sequence similarity between H. labeo (HD1) and H. mylodon was 88.91% and that of H. longirostis was 88.81% among the three species found in Korea. In addition, the nucleotide sequence similarities of H. maculatus, H. meditus, H. umbrifer and H. barbus showed 98.97%, 97.20%, 96.87% and 98.85%, respectively. Phylogenetic analysis on seven species of the genus Hemibarbus showed that the H. labeo collected in this study formed two clades. One of which consisted of Hadong, Imsil, Kangjin. The other one formed a step with HD2, HD8 and HD9 of Hadong and the H. labeo reported in Busan, Asan and Seoul, Korea. Phylogenetic position of the H. labeo among Cyprinidae showed 0.143 for the evolutionary distance from Zacco platypus and 0.006 for the H. maculatus. In addition, the genetic position of the H. labeo among 28 species of Cyprinidae was found to be located in Group I, including Gobioninae fishes. The results of this study will provide key genetic information for the taxonomic comparison in Cyprinidae and study of model fish for pollution monitoring in freshwater environments.

• Horticultural Science & Technology
• /
• v.31 no.6
• /
• pp.772-781
• /
• 2013

The objective of this study was to develop simple sequence repeat (SSR) markers from expressed sequence tags (EST) of lettuce (Lactuca sativa) and identify 9 germplasms from 3 wild species of lettuce and 61 commercial cultivars using the developed EST-SSR markers. A total of 81,330 lettuce ESTs from NCBI databases were used to search for SSR and 4,229 SSR loci were identified. The highest proportion (59.12%, 2500) was represented by trinucleotide, followed by dinucleotide (29.70%, 1256) and hexanucleotide (6.62%, 280) among SSR repeat motifs. Totally 474 EST-SSR primers were developed from EST and a random set of 267 primers was used to assess the genetic diversity among 9 germplasms and 61 cultivars. Out of 267 primers, 47 EST-SSR markers showed polymorphism between 7 cultivars. Twenty-six EST-SSR markers among 47 EST-SSR markers showed high polymorphism, reproducibility, and band clearance. The relationship between 26 markers genotypes and 70 accessions was analyzed. Totally 127 polymorphic amplified fragments were obtained by 26 EST-SSR markers and two to nine SSR alleles were detected for each locus with an average of 4.88 alleles per locus. Average polymorphism information content was 0.542, ranging from 0.269 to 0.768. Genetic distance of clusters ranged from 0.05 to 0.94 between 70 accessions and dendrogram at a similarity of 0.34 gave 7 main clusters. Analysis of genetic diversity revealed by these 26 EST-SSR markers showed that the 9 germplasms and 61 commercial cultivars were discriminated by marker genotypes. These newly developed EST-SSR markers will be useful for cultivar identification and distinctness, uniformity and stability test of lettuce.

• Journal of Life Science
• /
• v.19 no.2
• /
• pp.174-178
• /
• 2009

The characteristics of the population genetic structure of the Japanese anchovy (Engraulis japonicus Temminck & Schlegel) were collected from the West, South and East Seas of Korea in August, 2006 and were compared using six microsatellite DNA loci. In the West Sea population, the range of allele number against 72 individuals was from 19 to 41, the average allele number was 28.5. In EJ9, the allele number had the highest value of 41, this was 1.4 times higher than the average number of allele. The average allele number of the South Sea population was 24.5 that was less than that of West Sea population. In EJ2, EJ9 and EJ27.1 loci, the allele number was higher than average allele number in the South Sea population. In the East Sea population, the average allele number was estimated at 25.0 that most of loci except for EJ35 were higher than average allele number. Allele frequency in the West, South and East Sea populations was below 0.24. The value of observed heterozosity for six loci was approximately 0.5 higher than that of expected heterozosity (p>0.05), but three populations similar values to these heterozosity. Although the genetic diversity was higher value of above 0.9, three populations had a similar value. Genetic differentiation and distance combined estimate of the six loci were 0.258 and 0.019 (p>0.05), respectively, but showed no significant distance between three populations. These results suggested that it is responsible for no differentiated gene pool between three populations.

• Korean Journal of Ecology and Environment
• /
• v.55 no.3
• /
• pp.212-220
• /
• 2022

An estuary is a water ecosystem with a high abundance of the species diversity, due to a variety of complex physicochemical factors of the area where freshwater and ocean mixed. The identification of Corbicula species in the estuary environments is difficult because of various morphological characteristics. In this study, we provide taxonomic information on Corbicula species with taxonomic difficulties using morphological and genetic analysis. This study was conducted on clams from the Seomjin River-Gwangyang Bay, one of the major production area of marsh clam in Korea. As a result, we characterized Cytocrome C Oxidase subunit I (COI) sequences of the Corbicula. The 636 bp nucleotide sequences of COI have 98% homology among Corbicula species collected from 2 sites of Seomjin River-Gwangyang Bay. The phylogenetic analysis with 17 species of Corbicula indicated that most of the species collected from Seomjin River-Gwangyang Bay were brackish water clam (Corbicula japonica), and only one Asian clam (Corbicula fluminea). The evolutionary distance between C. japonica and C. fluminea was less than 0.003. Therefore, it was confirmed that C. japonica is phylogenetically closely related to C. fluminea. In 9 species of Cyrenidae, phylogenetic tree was classified into three lineages. These results will be used as an important data for an identification of clam species by providing genetic information for Corbicula species with a morphological diversity.