Animal Systematics, Evolution and Diversity

The Korean Society of Systematic Zoology (한국동물분류학회)
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Diversity of Mitochondrial DNA cytochrome b Gene in Two Subspecies of Striped Field Mouse, Apodemus asrarius coreae Thomas and A. asrarius manchuricus Thomas (Mammalia, Rodentia) from Korea and Northeast China

한국과 북동 중국에 서식하는 등줄쥐 2아종, Apodemus agrarius coreae Thomas and A. agirarius manchuricus Thomas (포유강, 설치목)의 미토콘드리아 DNA cytochrome b 유전자의 다양성

  • Koh, Hung-Sun (Department of Biology, Chungbuk National University) ;
  • Jinxing Wang (Department of Biology, Shandong University) ;
  • Lee, Bae-Kun (Department of Biology, Chungbuk National University) ;
  • Heo, Seon-Wook (Department of Biology, Chungbuk National University)
  • Published : 2001.04.01
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Abstract

The partial sequences of mtDNA cytochrome b gene in two subspecies of striped field mouse(Apodemus agrarius coreae and A. agrarius manchuricus) from Korea and northeast China were analyzed to determine the degree of genetic diversity in ech subspecies and to confirm their subspecific difference. In 18 specimens of A. agrarius coreae, ten haplotypes were resulted, and in two specimens of A. agrarius manchuricus. one haplotype was revealed. Tamura-Nei nucleotide distance among ten haplotypes in subspecies coreae ranged 0.36 to 1.86%. and nucleotide distance between two subspecies (coreae and manchuricus) was 0.37 to 1.47%: maximum infrasubspecific divergence in coreae was greater than maximum intersubspecific difference between two subspecies. Moreover, no major subgroup was resulted when 11 haplotypes in two subspecies were compared. Our sequence result was not cancordant with the morphological data studied so far, and it is concluded that cytochrome b gene sequence is not a good genetic marker to distinguish two subspecies of A. agrarius. In futurem, mtDNA control region analyses seemded to be necessary to reveal genetic relationship between these two subspecies of A. agrarius.

중국과 북동 중국에 서식하는 등줄쥐 2아종 (Apodemus agrarius coreae와 A. agrarius manchuricus)의 아종 내의 유전자 다양성의 정도를 파악하고 2아종간의 차이를 확인하기 위해, 미토콘드리아 DNA cytochrome b 유전자의 부분 염기서열을 분석하였다. 열 여덟마리의 A. agrarius coreae로부터 10 haplotype이, 그리고 2마리의 A. agrarius manchuricus로부터 1 haplotype이 밝혀졌다. 아종 coreae의 10 haplotype간의 Tamura-Nei nucleotide 거리는 0.36에서 1.86%였고, 2아종 coreae와 manchuricus간의 nucleotide 거리는 0.37에서 1.47%였다. 아종 coreae내 최대 genetic divergene 거리는 2아종간의 최대거리보다 크게 나타났다. 또한 2아종의 11 haplotype을 비교했을 때에, 뚜렷하게 아군으로 나뉘어지지 않았다. 본 염기서열의 분석 결과는 지금까지의 형태적 연구의 결과와 일치하지 않았으며, cytochrome b 유전자는 A. agrarius의 2아종을 구분하기에 좋은 유전자 marker가 아닌 것으로 판단됐다. 앞으로, A. agrarius의 2아종간의 유전적 관계를 밝히기 위해 미토콘드리아 DNA control region의 분석이 필요하다고 본다.

Keywords

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