• Environmental Analysis Health and Toxicology
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• v.17 no.4
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• pp.299-305
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• 2002

심폐 지구력은 유전인자에 의해 부분적으로 결정되며, 현재까지 이루어진 연구 결과에 의하면 안지오텐신 전환효소 유전자에 존재하는 다형성과 이 형질 사이에 유의한 관련성이 보고되고 있다. 그러나, 이러한 연구는 주로 서양인을 대상으로 수행되었기 때문에 유전적 배경이 다른 아시아 집단에 대해서는 아직까지 이렇다 할 연구 성과가 없는 실정이다. 이에, 본 연구에서는 아시아 집단중에서도 민족적으로 순수한 한국인 집단을 대상으로 안지오텐신 전환효소 유전자에 존재하는 다향성이 한국인 집단에서도 심폐 지구력과 유의한 관련성이 있는 지를 조사하였다. 그러나, 한국인 운동선수군을 대상으로 한 연구에서는 안지오텐신 전환효소 유전자의 다향성이 심폐 지구력을 비롯한 신체 계측치 및 생화학적인 측정치 들과 어떠한 관련성도 나타내지 않았다(P＜0.05). 그러나, 본 연구 대상으로 다양한 종목에서 선발된 운동선수들을 표본으로 하였기 때문에, 단일 종목의 운동 선수군을 대상으로 한 추시가 요구된다.

• Journal of Life Science
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• v.17 no.7 s.87
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• pp.882-888
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• 2007

Enzyme electrophoresis was used to estimate genetic diversity and population genetic structure of Pseudobagrus fulvidraco in Korea. Nine of the 14 loci (64.3%) showed detectable polymorphism. Genetic diversity at the population and species levels were 0.286 and 0.277, respectively. Analysis of fixation indices, calculated for all polymorphic loci in each population, showed a substantial deficit of hetero-zygotes relative to Hardy-Weinberg expectations. This deficit is expected that it is due to a limited effective number of individuals per population. The average $G_{ST}$ for polymorphic loci was 0.064, indicating that most (93.6%) of the genetic diversity occurred within populations. The indirect estimate of gene flow based on mean $G_{ST}$ was 3.67. Given limited gene flow is expected to diverge genetically due to drift and reduced populations. Most populations in our study experience annual, severe demo-graphic bottlenecks due to drought and floods.

• Exercise Science
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• v.21 no.1
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• pp.111-120
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• 2012

The purpose of the study was to assess the effects of different cardiorespiratory fitness response to exercise training on cardiovascular disease and adipocytokine release and gene expression in abdominal obesity women. forty eight middle-age women were divided to a exercise-responder (n=34) group (ERG) and a exercise-nonresponder (n=14) group (ENRG) based on cardiorespiratory fitness after exercise training (12weeks, 1200 kcal/week, moderate-vigorous intensity, walking and jogging). Cardiorespiratory fitness was measured using maximal oxygen uptake with metabolic gas analysis and body composition was determined by bioelectrical impedance. We measured lipid and glucose profiles, blood adipocytokines and adipocytokine genes expression in adipose tissue. Waist girth (p=0.040), and %body fat (p=0.031) were significantly decreased in ERG than ENRG. Triglyceride (p=0.023) and systolic blood pressure (0.046) were significantly decreased in ERG than ENRG. Blood leptin (p=0.022) was significantly decrease in ERG than ENRG but leptin gene expression was decreased both groups (p<0.001). These results show that exercise-responder group improved cardiovascular disease risk factors and adipocytokine more than exercise-nonresponder group after exercise training. Moreover, exercise-nonresponders group show that obese indices and blood leptin and leptin gene expression in adipose tissue were decreased despite the failure to improve fitness. Therefore, regular exercise training seems to give health benefits although the failure to improve fitness.

• Journal of Life Science
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• v.23 no.6
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• pp.728-735
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• 2013

Plantago asiatica (Plantaginaceae) is a wind-pollinated plant that grows mainly on fields in East Asia. Starch gel electrophoresis was used to investigate the allozyme diversity and population structure of 18 populations of this species. Although the plantain populations were isolated and patchily distributed, they maintained a high level of genetic diversity; the average percentage of polymorphic loci was 57.1%, the mean number of alleles per locus was 2.07, and the average heterozygosity for 18 populations was 0.201. The combination of a predominant wind-pollinated, mix-mating reproduction, large population sizes, high gene flow between subpopulations, and a propensity for high fecundity may explain the high level of genetic diversity within populations. A direct gradient in overall genetic diversity is associated with latitude. Genetic diversity of P. asiatica is markedly decreased from $35^{\circ}3^{\prime}$ to high latitude and decreased from $35^{\circ}3^{\prime}N$ to low latitude, whereas there does not show a longitudinal gradient in genetic diversity.

• Korean journal of applied entomology
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• v.40 no.3
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• pp.227-233
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• 2001

Four polymorphic allozymes were used for genetic analysis of overwintering populations of field diamondback moth, Plutella xylostella (Linne). Different allele frequencies were found among three local populations of Andong, Youngchon, and Yangsan in all loci. Two allozyme loci (acid phosphatase and phosphoglucomutase) showed significant deviation from Hardy-Weinberg equilibrium in allele frequencies among these populations. Estimated Nei's genetic distance varied from 0.0151 between Yangsan and Youngchon to 0.0877 between Andong and Youngchon. Compared with the previous genetic distances in this moth, a little higher genetic differentiation among these overwintering populations suggests that there would be a specific genetic bottleneck in each local population during overwintering period.

• Food Science of Animal Resources
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• v.24 no.1
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• pp.8-14
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• 2004

Identification of animals has been made with an ear tag with dummy code, and blood typing has been used for paternity and individual identification in live animals. As various genetic markers are for different cattle breeds vary, the discrete genetic markers are necessary to identify Hanwoo. A total of 740 progeny testing Hanwoo were used to identify Hanwoo specific markers. To examine traceability of individuals by using breed specific genetic codes, four animal were randomly sampled, and traced from live animals to post-slaughter processing stages. The candidate genetic makers used in the study were 16 DNA microsatellites which were identified in romosomes 1 and 14. The number of alleles of those DNA microsatellites ranged from a minimum of 3 to maximum of 12. The heterozygote frequency ranged from 0.022 to 0.824. Effective number of alleles for each DNA microsatellites were 3 to 6. Six selected candidate genetic markers were able ti trace individual cattle with an 100% confidence level.

• Proceedings of the Korean Institute of Intelligent Systems Conference
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• 2007.11a
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• pp.145-148
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• 2007

생명과학분야에서 마이크로어레이 기술은 세포에서의 RNA 발현 프로파일을 관찰할 수 있도록 함으로써 생명현상의 규명 및 약물개발 둥에서 분자수준의 생명현상에 대한 관찰과 분석이 가능 해지고 있다. 마이크로어레이 데이터분석에서는 특정한 처리나 과정에서 현저한 특성을 보이는 유전자를 식별하기 위한 분석뿐만 아니라 유전자 전체인 게놈수준에서의 분석도 이루어진다. 최근 유전자의 발현이 다양한 조절, 신호전달 및 대사경로에 의해서 영향을 받고 있다는 관점에서 게놈수준의 분석에 관심이 증가하고 있다. 약물반응 실험에서는 약물에 대한 게놈수준의 발현 프로파일을 관찰하는 것도 많은 정보를 제공할 수 있다. 약물실험에서는 대조군과 실험군들간에 관심 있는 상대적인 발현특성을 갖는 유전자군을 전체적으로 추출하는 것이 필요한 경우가 있다. 예를 들면 정상군은 두개의 실험군에 대해서 중간청도의 발현정도를 갖는 유전자군을 식별하는 분석을 하는 경우, 생물학적인 데이터의 특성상 절대값을 비교하는 방법으로는 유용한 유전자들을 효과적으로 식별해 낼 수 없다. 이 논문에서는 정상군과 실험군들의 발현정도값의 경향을 판단하기 위해서 각 유전자에 대해서 집단별 대표값을 선정하여 퍼지집합으로 집단의 값의 범위를 결정하고, 이를 이용하여 특정 패턴을 갖는 유전자들을 식별해내는 방법을 제안하고, 실제 데이터를 통해서 실험한 결과를 보인다.

• The Korean Journal of Malacology
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• v.26 no.4
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• pp.285-290
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• 2010

The nucleotide sequences of the mitochondrial cytochrome oxidase subunit 1 (CO1) gene of octopus groups collected from Muan, Taean, Yesu, Jeju in Korea and Youngsung, Daeryen in China were analyzed for the identification of species and populations. Six haplotypes were identified from the analyzed 60 individuals. All of the individuals (N = 10) from Jeju showed the A haplotype which was not observed from other groups, and could be classified as a distinct group. The analyzed groups could form two separate clade in MEGA4 analysis. The individuals from Muan, Taean, Yesu in Korea and Daeryen in China form a clase and the others from Jeju in Korea and Youngsung in China formed the other clade. The analysis of relationship among the groups showed the same results. Individuals belong to the group A (Muan, Taean, Yesu and Daeryen) showed closer relationship than individuals belong to the group B (Jeju and Youngsung). Although the CO1 universal primers used in this study was useful as a marker for species identification among Octopus, analysis of population was limited because of few variations in the partial sequences of CO1 analyzed in this study. However, it was possible to show the limited gene flow among the groups which is resulted from the spatial separation and differences in their habitats.

• Journal of Life Science
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• v.8 no.6
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• pp.687-694
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• 1998

Enzyme electrophoresis was used to estimate genetic diversity and population structure of Chimaphila japonica Miq. in Korea. The percent of polymorphic loci within the enzymes was 48.7%. Genetic diversity at the species level and at the population level was high (Hes=0.278 ; Hep=0.222, respectively), whereas the extent of the population divergence was relatively low ( $G_{ST}$ =0.079). $F_{IS}$ , a measure of the deviation from random mating within the 7 populations, was 0.355. An indirect estimate of the number of migrants per generation (Nm=2.61) indicates that gene flow is high among Korean populations of the species. In addition, analysis of fixation indices revealed a substantial heterozygosity deficiency in some populations and at some loci. Factors contributing to the high levels of genetic dive-rsity found in the entire species of C. japonica include wide distribution, long-lived perennials, ability to regenerate due to rhizomatous spread, outcrossing induced by animal vectors, and occasional pollen dispersal by wind.

• Proceedings of the Korean Information Science Society Conference
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• 2004.04b
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• pp.262-264
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• 2004

퍼지 클러스터링 방법은 일반적인 클러스터링 방법과는 달리 하나의 샘플이 다수의 집단에 속할 수 있으며 그 속하는 정도를 표현하여 보다 유연한 클러스터 분할의 분석을 가능하게 한다. 유전자 발현 데이터는 노이즈가 많고 공통된 기능을 가진 유전자들의 집단이 존재하기 때문에 퍼지 클러스터링을 사용하면 더욱 효율적으로 분석할 수 있다. 이러한 퍼지 클러스터링 방법에 있어서 중요한 것은 얼마나 분할이 정확하게 이루어졌으며 실제 데이터가 가지고 있는 분할과 결과가 얼마나 유사한가이다. 본 논문에서는 효과적인 유전자 클러스터의 평가를 위하여 베이지안 검증 방법을 제시하고, 결정트리로 생성된 규칙에 의하여 각 데이터의 특성에 따라 유연하게 검증하는 방법을 제안한다. 다양한 유전자 발현 데이터를 퍼지 c-means 알고리즘을 이용하여 클러스터링하고 제안하는 방법으로 검증한 결과, 그 유용성을 확인할 수 있었다.