• Title/Summary/Keyword: 원형이정

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Studies on the Induction of Transformation and Mutiplication in Orchid Plants (II) Isolation, Culture and Electroporation of Protoplasts in Bletilla striata (난과 식물의 형질전환 유도 및 다량증식에 관한 연구 (II) 자란의 원형질체 분리, 배양 및 Electroporation)

  • 이정석;김영준황성진황백
    • KSBB Journal
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    • v.6 no.2
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    • pp.201-205
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    • 1991
  • We have investigated influencing factors on viability of Bletilla striata protoplasts electroporated in the presence of various electrical conditions. Cultures of embryogenic callus and embryogenic cell suspension were established with immature seeds of Bletilla striata. Viabilty of electroporated protoplasts was decreased according to the increaseing of electroporation voltage and capacitance. An optimal condition of electroporation for viable protoplasts was in HBM buffer at $4^{\circ}C$.

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Omnidirectional Circularly Polarized Antenna Using Zeroth-Order Resonance (영차 공진을 이용한 전방향성 원형 편파 안테나)

  • Park, Byung-Chul;Lee, Jeong-Hae
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.20 no.8
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    • pp.806-812
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    • 2009
  • In this paper, the omnidirectional circularly polarized(CP) antenna using arc-shaped mushroom structure with curved branch is proposed. To obtain a vertical polarization and an omnidirectional radiation pattern, the CP antenna uses zeroth-order resonance(ZOR) mode of composite right and left handed(CRLH) transmission line. The horizontal polarization is achieved by the curved branches. Also, the spacing between curved branch and arc-shaped mushroom structure gives the $90^{\circ}$ phase difference between vortical and horizontal polarization. The proposed antenna, therefore, has an omnidirectional CP radiation pattern In the azimuthal plane. The electrical size of the proposed antenna is reduced by 38%, compared with that of the previously presented omnidirectional CP antenna. In addition, the CP antenna is simply designed without $90^{\circ}$ phase shifter and dual feed line. The proposed antenna uses a Bazooka balun for good impedance matching and radiation pattern. To improve 3 dB axial ratio in XY plane, the designed antenna is optimized. After optimization, the measured 3 dB axial ratio in XY plane is observed in $86{\sim}282^{\circ}$.

An Interval Type-2 Fuzzy C Spherical Shells Algorithm (Interval 제 2종 퍼지 C원형 윤곽선 알고리즘)

  • Hwang, Chul;Lee, Jung-Hoon
    • Proceedings of the Korean Institute of Intelligent Systems Conference
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    • 2004.04a
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    • pp.214-218
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    • 2004
  • 본 논문은 fuzzy C 원형 윤곽선(fuzzy C spherical shells 이하 FCSS) 알고리즘을 확장한 interval 제2종 fuzzy C원형 윤곽선 알고리즘에 관한 연구이다. 본 논문에서는 FCSS의 클러스터 윤곽선과의 관계에 의해 패턴이 할당 받은 퍼지 소속도(fuzzy 소속도) 값 결정에 존재하는 불확실성(uncertainty)은 표현하고, 관리하여 플러스터링 성능을 향상하고자 한다. 이러한 과정을 통하여 확장된 interval 제2종 FCSS는 패턴 집합에 존재할 수 있는 노이즈(noise)의 존재에 대해 기존의 FCSS보다 좀더 안정적이고, 바람직한 클러스터 윤곽선을 검출해낼 수 있도록 할 수 있을 것이다.

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Studies on the Induction of Transformation and Multiplication in Orchid Plants.(III) Expression of Gene Transferred into Orchid Protoplasts by Electroporation (난과식물의 형질전환 유도 및 다량증식에 관한 연구. III. Electroporation에 의해서 자란의 원형질체로 도입된 유전자의 발현)

  • 이정석;황성진김영준황백
    • KSBB Journal
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    • v.6 no.4
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    • pp.385-388
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    • 1991
  • Embryogenic cell suspension cultures of B. striata were established as transferred selected embryogenic callus into liquid medium. Protoplasts isolated from embryogenic cell suspensions were electroporated in buffered solutions containing plasmid DNA of pBI121. Transient GUS (beta-glucuronidase) activity measurement and selection for kanamycin resistent showed that expression of foreign genes and stable transformation were achieved. GUS transient gene expression was increased by increasing DNA concentration of pBI121 plasmid and affected by the level of the applied voltage. An optimal level of GUS activity was obtained after electroporation with a pulse of 200-300 voltage/1180 uF. Protoplast viability was up to the 80% at the optimal voltage.

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Effect of Drying method on the Fruit and Powder color of Red Pepper (건조방법이 고추 및 고춧가루의 색도에 미치는 영향)

  • Yoon, Wha-Mo;Lee, Jeong-Yeop
    • The Journal of Natural Sciences
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    • v.15 no.1
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    • pp.97-109
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    • 2005
  • This experiment was established to find out the effect of drying methods on the fruit and powder color of red pepper. Hunter's L, a, and b values of the dried fruit were also higher with the cut fruit in comparison with the whole or punched fruit. The Hunter's a value of the pepper powder after drying and grinding was slightly higher for the whole fruit with sun drying, but it was higher for the cut fruit in case of heat drying. The ASTA value was higher when pepper fruits were sun dried. For the heat drying, the value was higher with the drying temperature $60^{\circ}C$.

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-1 Mode Circular Polarization Antenna Design by Using Cross Aperture-Coupled Feed (십자 개구 결합 급전을 이용한 -1 모드 원형 편파 안테나)

  • Kim, Jun-Sik;Lee, Jeong-Hae
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.25 no.2
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    • pp.156-163
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    • 2014
  • In this paper, a compact circularly polarized metamaterial patch antenna using cross aperture-coupled feed is proposed. The CP antenna utilizes the -1 mode that is induced by the composit right-left handed(CRLH) transmission line. Since the -1 mode has the same properties with the $TM_{010}$ mode of the conventional patch antenna, the circular polarization(CP) can be realized. If two orthogonal modes are excited with $90^{\circ}$ phase difference, the CP property can be obtained. In order to obtain two orthogonal modes and $90^{\circ}$ phase difference, 4 mushroom structures having the shape of square are employed. The width and length of the cross aperture are optimized through the design algorithm. The fabricated antenna is based on RT/duroid5880 substrate and the total area of the 4 mushroom is $0.25{\lambda}_0{\times}0.25{\lambda}_0$. The center frequency of the LHCP(Left-Handed Circular Polarization) antenna is measured as 1.622 GHz and circular polarization bandwidth(3 dB) is measured as 3 MHz. The center frequency of the RHCP(Right-Handed Circular Polarization) antenna is measured as 1.609 GHz and circular polarization bandwidth (3 dB) is measured as 3 MHz, respectively. The measured radiation efficiency of LHCP antenna is 61.1 % and the measured radiation efficiency of RHCP antenna is 54.5 %.