• Journal of Korean Society of Forest Science
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• v.104 no.4
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• pp.578-587
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• 2015

Nuclear SSR (nSSR) and chloroplast SSR (cpSSR) markers were analyzed to assess the parameters of mating system in seed orchard, such as outcrossing rates, the number of potential pollen contributors, paternal contribution rates, degree of pollen contamination, and biparental inbreeding ($t_m-t_s$). In 2006, 2007, seeds were collected from the seed orchard of Pinus densiflora, established in 1977 at Anmyeon island. Estimates of outcrossing rates ranged from 94.9 to 100% (mean 98.9%) in 2006 and from 91.2 to 100% (mean 97.7%) in 2007 on the basis of the analysis of cpSSR haplotypes and from 90.3 to 100% (mean 95.9%) in 2006 and from 81.6 to 100% (mean 95.3%) in 2007 on the basis of the analysis of nSSR genotypes. By cross checking of both DNA markers, mean cumulative outcrossing rates of 100% and 98.9% were estimated in each year. Mean contamination rates were estimated as 48.9% and 42.4%, respectively. On the basis of cpSSR haplotype observed in each seed, paternal contribution rates (the number of pollen contributors) were estimated as 0.458 (mean 16.2) in 2006 and 0.512 (mean 14.8) in 2007. In conclusion, considering pretty high level of outcrossing rates observed in a seed orchard, there might be little to be influenced by inbreeding depression for genetic potential of the seeds induced by selfing. Estimates of the mating system parameters obtained from the two reproductive years were not statistically different, which revealed stable genetic quality of seeds produced in different years. Observed results from this study may provide useful information for the management and establishment of the seed orchard of the progressive generation.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.39 no.4
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• pp.259-269
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• 2013

In this work, licorice extracts were prepared using various extraction conditions such as extraction solvent, temperature, and time from Glycyrrhiza uralensis (G. uralensis) produced in Korea and China and Glycyrrhiza glabra (G. glabra) in Uzbekistan. The optimum extraction condition was selected from the extraction yields and antioxidative activities of extracts. Korea licorice extracts showed the highest free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activity (46.05%) under the extraction condition of 85% ethanol at $60^{\circ}C$ for 6 hours. The prominent ROS (reactive oxygen species) scavenging activity using luminol-dependent chemiluminescence assay and the cellular protective effect against $^1O_2$ induced cellular membrane damage were also shown from the extracts obtained from the same condition. Especially, Korea G. uralensis extracts exhibited the higher prominent protective effect (${\tau}_{50}$ = 116.4 min) than (+)-(+)-${\alpha}$-tocopherol (${\tau}_{50}$ = 28.5 min) and the extraction yield of Korea licorice extract was 18.75%, which is 1.2 times and 2.5 times higher than that of Uzbekistan and China, respectively. These results indicate that the extraction condition of 85% ethanol at $60^{\circ}C$ for 6 hours is optimal to prepare licorice extracts, which can be applicable as anti-oxidative cosmetic materials.

• Journal of the Korean Chemical Society
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• v.53 no.6
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• pp.742-748
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• 2009

In the meat industry, correct breed information in food labeling is required to assure meat quality. Genetic markers provide corroborating evidence to identify breed. We described the development of DNA markers to discriminate between Korean beef cattle (Hanwoo), Holstein, and mixed cow beefs. As most breeds are standardized for coat colour, the melanocortin 1 receptor (MC1R) gene, involved in the regulation of eu/pheomelanins synthesis, has been suggested as marker for breed traceability of products of animal origin. We also designed sex-determining region Y (SRY) gene specific primers for Y chromosome detection. In this study, fragments of MC1R gene and SRY gene were amplified by multiplex-PCR and subjected to digestion by MspA1I restriction endonuclease. Reaction products were analysised by denaturing high performance liquid chromatography (DHPLC). As a result, we identified 6 DHPLC peak types from MC1R gene and SRY gene analysis. DHPLC method showed more sensitive than RFLP method for DNA fragments analysis. Therefore, DHPLC method can apply to identify for Hanwoo, Holstein and mixed beef.

• Journal of Bio-Environment Control
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• v.27 no.3
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• pp.245-252
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• 2018

Yam bean (Pachyrhizus erosus) is a subtropical plant belonging to the Fabaceae family, and is a tuberous vegetable used as various food material with a crisp and juicy taste. This study was conducted to seek optimum sowing time of yam bean in Korea. For this, we surveyed germination properties by the different temperatures and compared the accumulation temperature (AT) and dry matter production (DMP) on growth stages of yam bean by the different sowing times. Two types of varieties cultivated mainly in Korea, Thailand local variety (TLV) and Cheongunmanma cultivar (CGMM), was used. The germination rate of yam bean was 86.0~94.0% at above $18^{\circ}C$, and germination days was longer at lower temperature. The times for flowering and tuber formation of CGMM were later than those of TLV, and the AT required for flowering, tuber formation and hypertrophy of CGMM were higher than those of TLV by $293^{\circ}C$, $280^{\circ}C$ and $108^{\circ}C$, respectively. Also, DMP of shoot and tuber in CGMM were greater than those in TLV. In sowing at April 25, tuber formation was slower than sowing after that time, and harvest index (HI) was relatively low due to delayed formation and hypertrophy of tuber. In sowing after June 9, DMP of shoot was relatively greater in early growth, but tuber was not sufficient to enlarge due to lack of growth days by cold and frost in late October. In sowing May 10 and 25, DMP of tuber and HI were the highest, because the change of day length and temperature gave an advantage to vegetable growth and tuber development. All above suggest that it was suitable to sow seeds on May for increment of tuber productivity in Korea.

• Korean Journal of Plant Resources
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• v.23 no.1
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• pp.60-78
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• 2010

To clarify the distribution of vascular plants and their usefulness in Heoninlleung, Ecological Landscape Conservation Areas of Seoul, we investigated it from April, 2006 to June, 2009. Total 313 taxa; 68 families, 191 genera, 264 species, 41 varieties and 8 forma were distributed in Heoninlleung. Among them, 37 taxa were highly abundant everywhere (3A), 16 taxa were highly abundant locally (3B), 70 taxa were moderately abundant everywhere (2A), 96 taxa were common in certain regions locally (2B), 9 taxa were rare but observed everywhere with low frequency (2A) and 85 taxa were rare and observed locally (1B). The economic plants were 293 taxa. There were 156 taxa of edible source, 223 taxa of medicinal source, 141 taxa of ornamental source, 69 taxa of pastoral source, 12 taxa of industrial, and 8 taxa of timber source. Twelve Korean endemic plants were collected. Based on the list of rare plants by the Korea National Arboretum and Ministry of Environment, 2 rare species were found. The specific species of I~V grades by phytogeography were 19 taxa. And twentyfour taxa of naturalized plant species were distributed. Naturalization Index was 7.7% and Urbanization Index was 8.4% in the investigated area.

• Korean Journal of Food Science and Technology
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• v.44 no.4
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• pp.401-410
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• 2012

This study examines the authenticity discrimination of the circulated honey by using stable isotope ratio methods. In the case of domestic honey, the range of ${\delta}^{13}C$ for the samples labeled as pure honey was about -27- -21‰ at the $C_3$ origin, and the range of that for artificial honey was over -19‰ at the $C_4$ origin. The range of ${\delta}^{13}C$ for all imported honey was over -27- -23‰ originating from the $C_3$ plant. According to the nectar-source, ${\delta}^2H$ and ${\delta}^{18}O$ for domestic honey were significantly different for 6 and 5 groups, respectively. However, we could not explain the detailed relationship as well as the geographical feature of ${\delta}^2H$ and ${\delta}^{18}O$. The difference for ${\delta}^2H$ and ${\delta}^{18}O$ in the wide range of latitude, such as between Australia and Canada, was more or less shown. However, it was difficult to find out the trends of ${\delta}^2H$ and ${\delta}^{18}O$ for imported honey versus the geographical information in the similar latitudinal country.

• Journal of the Korean Society of Food Science and Nutrition
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• v.34 no.6
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• pp.915-923
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• 2005

We monitored paralytic shellfish poison (PSP) by HPLC method in shellfishes including a sea squirt, and its products produced in domestic and imported, total 35 species, 850 samples, collected at 9 cities such as Seoul, Busan, Daegu, Ulsan, Pohang, Masan, Tongyeong, Geoje and Sacheon, 10 times by month (2 times in April and May) from March to October, 2004. PSPs were detected in a few raw samples on March, and it showed highest toxic ratio $(72.9\%)$ on third week of April (51 samples toxic in total 70 samples) and decreased suddenly to $2.4\%$ on third week of May. After then, PSPs were detected in a few samples, sporadically, and disappeared after August. Most raw shellfishes in the market were safe from PSP, showing the toxicity below 1 MU/g (quarantine level 4.0 MU/g), except oyster collected on April at Masan (1.9 MU/g), blue mussel on April (1.8 MU/g) and purplish washington clam on May at Sacheon (2.1 MU/g). However, it was strongly suggested to survey and under control for the imported scallops and ark shells which showed highly toxic in the quarantine level on May, July, even September. PSP were not detectable in the all shellfish products collected on May, July and September, except 2 boiled and dried mussels contained trace amount (0.01 MU/g), and all those products were safe as below quarantine toxin level (4.0 MU/g).

• Journal of the Korean Society of Food Science and Nutrition
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• v.45 no.11
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• pp.1533-1543
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• 2016

The anti-stress effects of Punica granatum L. (family Lythraceae, PG) on $H_2O_2$/corticosterone (CORT)-induced stress in cells and sleep-deprived rats were investigated. The PG extract showed neuroprotective effects in SH-SY5Y cells against $H_2O_2$/CORT-induced stress. Sleep deprivation led to behavioral, hormonal, and biochemical alterations in the animal model. The effects of P. granatum on physiological, behavioral, and biochemical parameters aggravated by sleep deprivation were investigated. Sleep deprivation impaired physiological (survival, body weight, and drowsiness scores) and behavioral (rotarod, passive avoidance, hot hyperalgesia, and Y maze) parameters as well as biochemical factors (cortisol, serotonin, dopamine, testosterone, and growth factor I contents in serum). These parameters were significantly recovered by PG extract in a concentration-dependent manner. The PG extract also enhanced catalase, superoxide dismutase, and non-enzymatic antioxidative activities such as glutathione compared to sleep-deprived rats. On the basis of these results, our findings suggest that Punica granatum prevents impairment of body functions induced by sleep deprivation and related oxidative damage.

• Journal of the Korean Institute of Landscape Architecture
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• v.45 no.6
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• pp.28-39
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• 2017

The purpose of this study was to analyze the tendencies of the written test questions regarding the scientific plant names in the subject of scenic planting in the national qualification test for landscape engineers. To achieve this purpose, targeting the 20 questions in the subject of scenic planting, the study chronologically analyzed the types of the presented questions about the scientific names of the plants, the changes in their year-to-year number, and their presentation frequency over the past 11 years from 2006 to 2016. The following was found by an analysis of the tendency of the questions presented over 11 years from 2006 to 2016 regarding the scientific plant names in scenic planting in the written test of the national technical qualification examination for landscape engineers. The number of these questions was 234, and they were classified into 13 items: scientific names, families/genuses/ species, characteristics, barks, roots, foliage, flowers, fruits, properties, places of origin, stalks, leave, and uses. After analyzing the examination question trends per year, the year in which the highest number of questions was given was 2013 at 56.6%; the ratio of questions asking botanical names increased until 2013, although that ratio decreased starting in 2014 due to consistent efforts from the landscaping field. The plant species most frequently asked about were Betula platyphylla and Taxus cuspidata and others frequently asked about included Pinus densiflora, Acer palmatum, Forsythia koreana, Cercis chinensis, Robinia pseudoacacia, Cornus officinalis, Zelkova serrata, Abies holophylla, Camellia japonica, Sophora japonica, Chaenomeles sinensis, which were asked about more than 10 times. The number of plant species presented as question items or choice items was 240 and their presentation frequency was 806, Among these, 66 species were steadily presented more than five times. They were presented 486 times, accounting for 27.5% of all species. The number of the new plant species which began to be presented in 2009 were 119(49.58% including Quercus myrsinaefolia) of all 240 species, indicating that their presentation frequency was gradually increased. As a result of comparative analysis of questions on arbor and shrub, evergreen and leaf abscission that have been presented for recent 11 years, the questions on arbor were more than shrub, the questions on leaf abscission were more than evergreen.

• Journal of Korea Fair Competition Federation
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• no.150
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• pp.14-29
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• 2010

비록 부정경쟁방지법의 제정 목적이 부정경쟁행위 등의 방지를 통하여 건전한 거래질서를 유지한다는 의미의 경쟁체제 확립에 있기는 하지만, 우리나라의 경우는 법 제정 당시와는 달리 사실상 산업스파이에 대한 영업비밀의 보호나 주지의 상표 영업표지의 보호와 같은 지적재산권의 보호 법률로서의 역할로 점차 변화하고 있고, 특히 부정경쟁방지법이 주지의 상표에 대한 출처의 혼동에 대한 규제뿐만이 아니라 별도로 저명상표의 희석화(稀釋化) 방지라는 법익, 이에 더 나아가 도메인 네임(Domain Name)의 선점과 원산지 및 품질의 오인(誤認) 야기행위, 주지 저명한 타인의 디자인(Design), 캐릭터(Character)와 같은 상품의 표지에 이르기까지 지적재산권과 관련된 보다 넓은 법익의 보호까지 수행하게 되면서 그 기능은 날로 강화되고 있는 상태이다. 이에 반하여 부정경쟁방지법상의 부정경쟁행위 자체에 대한 규제는 사실상 주지 저명한 타인의 상표나 상품표지의 식별력이나 출처표시기능 등의 보호라는 의미의 분쟁수준을 넘지 못하고 있어, '경쟁법'으로서의 역할은 상대적으로 미약해지고 있는 것 또한 현실이다. 또한, 공정거래법 제23조 제1항 제8호를 비롯하여 현행 공정거래법상의 불공정거래행위에 대한 규정체제를 살펴보면, 해석 여하에 따라서는 부정경쟁방지법상의 부정경쟁행위가 대부분 공정거래법상의 불공정거래행위의 범위 내로 포섭될 수도 있는 상황이기도 하다. 이에 양 법률의 성격과 역할, 앞으로 나아가야 할 방향을 고민하지 않을 수 없고, 이와 같은 논의는 발전적 입법론으로서의 가치를 갖는다. 물론 불공정거래행위(부정경쟁행위)에 대한 규제에 있어서 반드시 독일법체계에 따를 것인지 아니면 미국의 경우를 따를 것인지에 대한 선택 자체가 논리적으로 양립 불가능한 것은 아닐 것이지만 우리나라의 경우는 1980년 "독점규제 및 공정거래에 관한 법률"이 제정되는 과정에서 당시 부정경쟁방지법에 담겨 있던 기존의 부정경쟁행위에 대한 규정과 공정거래법상의 불공정거래행위와의 경합이나 중복문제는 마땅히 검토되었어야 했음에도 불구하고 공정 거래법의 제정과정에서 사실상 부정경쟁방지법의 존재 자체가 간과되어 오늘에 이르고 있다. 그동안 양 법률상의 규정 중복이나 충돌을 정식으로 문제 삼았던 바는 없었지만 '발전적 입법론' 이라는 차원에서 살펴 보면 부정경쟁방지법상의 부정경쟁행위에 대한 규제는 앞으로 공정거래법체계 내의 불공정거래행위로 포섭할 필요가 있고 이를 통하여 경쟁정책의 전문 전담기구로서 불공정거래행위에 대한 규제의 중심에 서 있는 공정거래위원회를 중심으로 효율적이고 통일적인 경쟁정책을 확립을 기대하여 볼 수 있을 것이다. 이 과정에서 공정거래법의 변화 또한 뒤따라야 하는데, 부정경쟁방지법상의 부정경쟁행위의 편입에 따라 불공정거래행위에 대한 규정 일부를 알맞게 다시 수정하는 것에 그치지 않고, 기존 부정경쟁방지법이 인정하고 있었던 사인간(私人間) 금지 또는 예방청구권 또한 공정거래법으로 그대로 편입되는 방향으로의 입법 개선이 이루어질 필요가 있으며, 그동안 '부정경쟁방지법의 공정거래법으로의 편입문제'와는 전혀 무관하게 공정거래법의 사적 구제 및 사소(私訴)의 활성화 차원의 논의로서 공정거래법상 사인간 금지청구권의 도입 여부가 검토되어 왔지만, 앞으로 이 문제는 부정경쟁방지법상 부정경쟁행위의 공정거래법체계 내로의 편입문제와 함께 이를 포함한 더욱 큰 논의로서 다시 적극적으로 검토될 필요가 있다고 본다. 이를 통하여 앞으로 부정경쟁방지법은 특허청을 중심으로 산업스파이에 대한 규제나 영업비밀의 보호와 기타 지적재산권의 보호에 온 힘을 다하고, 공정거래법은 공정거래위원회를 중심으로 불공정거래행위 (부정경쟁행위에 대한 보다 포괄적이고 통일적인 규제를 담당하여 '선택과 집중' 이라는 차원의 각 법률체계의 한 차원 높은 발전 또한 기대해 볼 수 있을 것으로 확신한다. 이러한 합의점을 시작으로 미시적인 다음 단계의 논의에 해당하는 사인간 금지청구권의 허용범위나 허용요건, 남용을 방지하기 위한 제도적 장치, 단체소송 등의 허용 여부 등의 논의도 함께 하여야 할 것이고, 이 과정에서 미국의 클레이튼법(Clayton Act)이나 가까운 일본의 입법례를 참고하여 우리의 실정에 맞는 규제의 틀을 마련함이 타당할 것이고, 이를 통하여 궁극적으로 그동안 공정거래법의 사적 집행의 활성화를 통한 경쟁질서의 확립의 강화라는 이상에 더욱 가까워질 수 있는 좋은 입법적 변화의 모습을 볼 수 있을 것이라 생각한다.