• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.9
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• pp.559-568
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• 2020

Epidermal growth factor (EGF) is a hormone protein that affects cell growth and proliferation, and has various medical applications. In the present study, the gene of human EGF was codon-optimized with E. coli and the expression vector was constructed by cloning into pRSET. In order to obtain the recombinant human EGF in an active form rather than an inclusion body, chaperone co-expression was attempted along with codon optimization, for the first time. The expressed human EGF was isolated in the pure form by performing Ion Exchange Chromatography in two consecutive runs. ELISA analysis showed that the activity of purified EGF was greater than 99%, which is similar to commercially available EGF. Cell proliferation test confirmed that the recombinant human EGF has the ability to promote cell proliferation of human skin fibroblasts. The human EGF expression system of this study gives a significant amount of protein, and does not require the renaturation step and the additional chromatographic system to remove a fusion contaminant, thereby providing a very useful alternative to conventional expression systems for the preparation of recombinant human EGF.

• Applied Chemistry for Engineering
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• v.18 no.5
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• pp.475-482
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• 2007

The thermal and optical properties of glucose penta(cholesteryloxycarbonyl)alkanoates (CAGLn, n = 2~8, 10, the number of methylene units in the spacer) were investigated. All the CAGLn formed monotropic cholesteric phases with left-handed helical structures. CAGLn with n = 2 or 10, in contrast with CAGLn with $3{\leq}n{\leq}8$, did not display reflection colors over the full cholesteric range, suggesting that the helical twisting power of the cholesteryl group highly depends on the length of the spacer joining the cholesteryl group to the glucose chain. The isotropic-cholesteric transition point ($T_{ic}$) decreased with increasing n and showed no odd-even effect. The plot of transition entropy at $T_{ic}$ against n had a sharp negative inflection at n = 7. The optical pitches (${{\lambda}_m}^{\prime}$ s) of CAGLn with $3{\leq}n{\leq}8$ decreased with increasing temperature. However, the temperature dependence of the ${\lambda}_m$ of the derivatives exhibited pronounced dependence on n. The transitional properties and the temperature dependence of the ${\lambda}_m$ observed for CAGLn were discussed in terms of the differences in arrangement of the cholesteryl groups and the conformation of the molecules.

• Journal of Korean Society for Geospatial Information Science
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• v.18 no.4
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• pp.141-149
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• 2010

Three-dimensional geo-spatial information is important for the efficient use and management of the country and the three-dimensional expression and analysis of urban projects, such as urban plans devised by local governments and urban management. Thanks to the revitalization of the geo-spatial information service industry, it is now being variously used not only in public but also private areas. For the creation of high-guiltily three-dimensional geo-spatial information, emphasis should be placed on not only the quality of the source image and three-dimensional geo-spatial model but also the level of visualization, such as level of detail and texturing. However, in the case of existing three-dimensional geo-spatial information, its establishment process is complicated and its data are not updated frequently enough, as it uses ready-created digital maps. In addition, as it uses Ortho Images, the images exist Relief displacement. As a result, the visibility is low and the three-dimensional models of artificial features are simplified to reach LoD between 2 and 3, making the images look less realistic. Therefore, this paper, analyzed the quality of three-dimensional geo-spatial information created using the three-dimensional modeling technique were applied using Digital photogrammetry technique, using digital aerial photo images by an existing large-format digital camera and multi-looking camera. The analysis of the accuracy of visualization information of three-dimensional models showed that the source image alone, without other visualization information, secured the accuracy of 84% or more and that the establishment of three-dimensional spatial information carried out simultaneously with filming made it easier to gain the latest data. The analysis of the location accuracy of true Ortho images used in the work process showed that the location accuracy was better than the allowable horizontal position accuracy of 1:1,000 digital maps.

• Journal of Korean Society of Environmental Engineers
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• v.39 no.11
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• pp.607-612
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• 2017

The effect of carbonic anhydrase on $CO_2$ absorption rates and the heat of reaction were evaluated in various amine solutions for post combustion $CO_2$ capture process. The $CO_2$ absorption rate was analyzed in 30 wt% MEA, AMP, DMEA, MDEA aqueous solutions with and without carbonic anhydrase (250 mg/L) from bovine erythrocyte. $CO_2$ absorption rates were increased in all solutions with carbonic anhydrase. The effect of carbonic anhydrase on absorption rates was more in tertiary amine (DMEA and MDEA) solutions than in primary amine (MEA) and hindered amine (AMP) solutions. The heat of reaction of MEA, DMEA, MDEA aqueous solutions with and without carbonic anhydrase were measured using reaction calorimeter. Carbonic anhydrase decreased the heat of absorption in all solutions. The results suggested that tertiary amines that have the excellent desorption ability were suitable for applying carbonic anhydrase to the post combustion $CO_2$ capture process and the effect of carbonic anhydrase was best in MDEA solution.

• Spatial Information Research
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• v.23 no.2
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• pp.49-57
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• 2015

Recently, Through the linkage and opening, the fusion of the spatial information, it is necessary for productive ecosystem to provide a variety of information and to increase the civil use. Depending on the economic growth, demand for quality of life and well-being has been on the increase. Spatial information service contents for the public convenience has emerged to solve the problem such as health, safety, welfare and discomfort of daily life This study aims to implement search services for a tailored residence space through the three-dimensional data modeling on cadastral information. To achieve this goal, we established the requirements for deriving a registered object by investigating recent trend with respect to existing cadastral data model and defined property and relationship. Focusing on Songpa-gu, Jamsil station in Seoul, we implemented search services for a tailored residence space for three-dimensional right analysis in conjunction with residential and commercial complex building. As a result, we derived a way to supply 3D cadastre information through open platforms (VWorld) and to represent efficiently, which is able to improve the quality of spatial information service contents for the public convenience as well as to widen utilization of information.

• Polymer(Korea)
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• v.32 no.3
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• pp.230-238
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• 2008

The thermal and optical properties of cellobiose octa(cholestryloxycarbonyl)alkanoates CCCBn, $n=2{\sim}8$,10, the number of methylene units in the spacer) were investigated. All the samples formed monotropic cholesteric phases with left-handed helical structures. CCBn with n=2 or 10, in contrast with CCBn with $3{\leq}n{\leq}8$, did not display reflection colors over the full cholesteric range, suggesting that the helical twisting power of the cholesteryl group highly depends on the length of the spacer connecting the cholesteryl group to the cellobiose chain. The isotropic-cholestropic transition ($T_{ic}$) and glass transition temperatures decreased with increasing n and showed no odd-even effect. The transition entropy at $T_{ic}$ increased with increasing n from 2 up 6, but at n=7 it drops significantly and then increased again with increasing n from 8 to 10. The sharp change at n=7 may be attributed to a difference in arrangement of the side groups. The thermal stability and degree of order in the mesophase and the temperature dependence of the optical pitch observed for CCBn were significantly different from those reported for the cellulose tri(cholesteryloxycarbonyl)alkanoates and glucose penta(cholesteryloxycarbonyl)alkanoates. The results were discussed in terms of the differences in the degree of polymerization, the number of the mesogenic units per mole-glucose unit, and the conformation of the molecules.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.15 no.2
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• pp.36-49
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• 2016

A subway system is one of the major transportation modes at a metropolitan area. When it meets the other lines, the metro station, so-called transferring station, is usually threatened by severe pedestrian congestion and safety issue of transit users including the transportation vulnerable. Although transportation planners forecast travel demand at the beginning, it is not easy to predict pedestrian flows precisely for a long term if land use plans have dramatically changed. Due to expensive costs, structural extension of metro stations is limited. Therefore, it requires efficient and technical improvements as meeting the demand of pedestrian and physical characteristics. In this study, the core mechanism of pedestrian movement-based simulation model was introduced and evacuation scenarios were analyzed with the developed model. As a result, the multiple optimal routes for unexpected events at the solid space of the multiple stories are easily searched through the simulator and in the case of Sadang Station, travel time can be reduced by 60% when the evacuation information and intuitive design are provided.

• 한국노년학
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• v.37 no.2
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• pp.475-496
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• 2017

Increasing evidence shows that ageist attitudes towards older adults develop quite early in childhood in Western culture, yet, empirical research on this topic in Korea is lacking. It has been argued that as Korea has become more youth oriented, negative views of aging have emerged and Korean children are increasingly exposured to negative stereotypes of older adults. Nuclearization of family structure and consequent reduction of intergenerational interaction is considered to be another important factor. The purpose of this study is to find out whether Korean children have ageist attitudes. Acknowledging the possibility that children tend to report in socially appropriate ways to the explicit measures of ageism, we included implicit measures of ageism. Data was collected from 570 fourth to sixth grade elementary school children selected by quota sampling. The current study used Child-Age Implicit Association Test and two other explicit measurements. The original versions were modified to be culturally applicable to the Korean context. Results indicated that when measured explicitly, children do not report preferring younger person than older person. However when measured implicitly, it was found that children at this young age already reveal negative biases toward the elderly. Contrast to the fact that the level of explicit ageism is significantly different by demographic characteristics of children, no statistical difference in the level of implicit ageism is detected. Based on the results, implications are discussed.

• Journal of Digital Convergence
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• v.19 no.4
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• pp.251-257
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• 2021

With the development of new media, great changes are taking place in the way people get information. The change is the use of video content that can deliver content in a more three-dimensional way than words or photos. After 2016, the number of live video streaming content providers and users has increased. In this paper the write takes the 1 personal live video streaming content as the research object. And the write takes live video streaming content on YouTube live or Douyu TV as a research example. In this paper, the writer analyzes the digital information content in the live video streaming case. And the writer expounds the necessity of these visual information and the characteristics of real-time live video streaming content. Especially since 2020, because of the influence of the COVID-19, the live video streaming industry has begun to combine with the traditional industry. It is expected that the integration of digital cutting-edge technology and live video streaming will not only provide diversity in the content, but also create more social value for the video content consumption culture. Therefore, The writer thinks it is necessary to conduct in-depth research on the social responsibility of real-time live content in the future.

• Journal of the Korean Society of Radiology
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• v.16 no.4
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• pp.471-476
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• 2022

The purpose of this study was to study the probability of cancer occurrence due to photoneutron dose exposure of the colon and thyroid gland, which are normal organs, in 3D CRT, IMRT 5 portals, and IMRT 9 portals, which are radiotherapy methods for prostate cancer. The total prescribed dose for prostate cancer was 6600 cGy, 220 cGy per dose, and 30 divided irradiations were applied for the total number of times. After setting up the Rando phantom on the treatment table (couch) of the medical linear accelerator used in the experiment, an optically stimulated luminescence albedo neutron dosimeter was placed on the corresponding area of the large intestine and thyroid gland of the phantom for measurement. During 3D CRT of prostate cancer, the probability of secondary cancer due to photoneutron dose to the colon and thyroid gland, which are normal organs, was 1.8 per 10,000 people. And IMRT 5 portals were 8.7 per 10,000 people, which was about 5 times larger than 3D CRT. IMRT 9 portals derived the result that there is a probability that 1.2 people per 1,000 people will develop cancer. Based on this study, the risk of secondary radiation exposure due to the dose of photoneutrons generated during radiation therapy is studied, and it is thought that it will be used as useful data for radiation protection in relation to the stochastic effect of radiation in the future.