• Applied Microscopy
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• v.30 no.1
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• pp.45-59
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• 2000

Cis-platin is a widely used anticancer drug against certain solid tumors such as malignant ovarian tumor, malignant carcinoma of head and neck, bladder cancer and cervical cancer of uterus, and its major mechanism of action is inhibition of DNA synthesis of the tumor cell. To investigate the inhibitory effects of cis-platin on the ciliogensis of the ciliated cells in the mucosa of oviduct, the author pursued the alterations of $\alpha-tubulin$, which is the main constituent of the microtubles in cilia, after cis-platin treatment. To eliminate the possible variations due to ovarian cycle, female Spargue-Dawley rats ($150\sim200gm$ in B.W.) were pretreated with estradiol benzoate (20 mg/kg, once a day, for 4 consecutive days). Animals were administrated with cis-platin (6 mg/kg, i.p.) and sacrificed at 1day, 3days, 5days and 7days after treatment, respectively. Immunohistochemistry for $\alpha-tubulin$ using mouse anti-rat $\alpha-tubulin$ monoclonal antibody as primary antibody was done. Immunogold electronmicroscopy for intracellular distributions of $\alpha-tubulin$ was also performed with same primary antibody and Goat anti- mouse IgM which is preconjugated with gold particles of 15 nm as secondary antibody. The results obtained were as follows; 1. Strong immunoreactivity of $\alpha-tubulin$ was observed in ciliated cells of oviducts at 1, 3 and 5 days after estradiol pretreatment. 2. Weak immunoreactivity of $\alpha-tubulin$ was observed in ciliated cells of oviducts at 1 and 3 days after cis-platin treatment but it was recovered to strong immunoreactivity in 5 days 3. In immunogold electronmicroscopy, density of gold particles for $\alpha-tubulin$ reactions was decreased in apical cytoplasm, but few changes were observed in basal body or cilia at 1 and 3 days after cis-platin treatment. From these above results, it is indicated that synthesis of $\alpha-tubulin$ in ciliated cells of rat oviduct is inhibited by cis-platin treatment.

• Korean Journal of Agricultural Science
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• v.19 no.2
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• pp.201-237
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• 1992

본(本) 연구(硏究)는 1992년(年) 2월(月)부터 동년(同年) 8월(月)까지 한국(韓國)의 농어촌진흥공사(農漁村振興公社)(RDC)와 말레시아의 KTA기술용역단간(技術用役團間)의 기술제휴하(技術提携下)에 아세아개발은행(亞細亞開發銀行)의 기술협력기금(技術協力基金)에 의(依)한 말레지아북부(北部) Terengganu 농촌개발제(農村開發第)2단계사업(段階事業)의 타당성조사연구(妥當性調査硏究) 결과(結果) 중 말레시아정부(政府)의 경제정책방향(經濟政策方向), 농업개발(農業開發) 및 사업(事業)의 경제적(經濟的) 재무적(財務的) 타당성(妥當性)만을 발췌(拔萃)하여 본(本) 논문(論文)에 수록(收錄)하였다. 본(本) 농촌개발사업지구(農村開發事業地區)는 말레시아의 북동(北東)쪽에 위치(位置)하고 있는 Terengganu State의 Setiu-Besut양(兩) Distict가 포함(包含)되는데 경제적(經濟的)으로 가장 낙후(落後)된 지역(地域)으로 매년(每年) 행사(行事)처럼 찾아오는 홍수(洪水)때문에 농경지(農耕地)를 집약적(集約的)으로 경작(耕作)하지 못함으로써 지역농민(地域農民)들은 빈곤(貧困)에서 벗어나지 못하고 있고 침수기간중(浸水期間中)에는 지역(地域)의 경제활동(經濟活動)은 물론(勿論) 교통(交通)마저 불통(不通)되고 농촌하부구조(農村下部構造)의 손실(損失)은 물론(勿論) 사회경제적(社會經濟的)인 손실(損失)이 크며 Setiu River의 하구(河口)가 침전(沈澱)되어 어선(漁船)의 출입(出入)이 점점(漸漸) 곤란(困難)해지므로써 어민(漁民)들의 생계(生計)에도 위협(威脅)을 주는 지역(地域)이다. 따라서 본(本) 타당성(妥當性) 조사연구(調査硏究)의 근본적(根本的)인 목적(目的)은 농촌(農村)의 빈곤(貧困)을 타파(打破)하기 위하여 (1) 홍수(洪水)를 방지(防止)하고, 배수(排水) 및 관개개선(灌漑改善)을 하며, 하구(河口)를 개발(開發)하여 어민(漁民)들의 생산활동(生産活動)을 돕고 지역주민(地域住民) 및 농민(農民)들의 경제활동(經濟活動) 및 농업생산성(農業生産性)을 제고(提高)시키며 (2) 환경보호(環境保護) 및 관리(管理)를 통(通)하여 지역주민(地域住民)에게 쾌적(快適)한 농촌생활환경(農村生活環境)을 제공(提供)하고 생태계(生態系)의 변화(變化)를 방지(防止)하며 (3) 다각적(多角的)인 영농활동(榮農活動)을 통(通)하여 지역농민(地域農民)의 소득(所得)을 극대화(極大化) 할 수 있는 개발(開發)의 기본구상(基本構想)과 이에 대한 기술적(技術的) 경제적(經濟的) 타당성(妥當性)을 구명(究明)하는 것이다. 본(本) 사업지역(事業地域)의 총면적(總面積)은 9,500ha이며 이는 4,680ha의 기설지구(旣設地區)의 개보수관개사업(改補修灌漑事業)과 500ha의 과수단지(果樹團地), 200ha의 채소단지(菜蔬團地), 500ha의 옥수수단지(團地), 250ha의 엽연초생산단지(葉煙草生産團地), 2,760ha의 오일팜 및 고무나무단지에 소, 염소 및 양(洋)을 사육(飼育)하는 종합적(綜合的)인 농촌개발(農村開發)로서 농가(農家)의 농업소득제고(農業所得提高)에 큰 기여(寄與)를 하게 되며 Setiu강(江)의 유역(流域) 4,090ha에 대한 홍수경감대책(洪水輕減對策)으로 지역주민(地域住民)의 생활안정(生活安定) 및 교통(交通), 관광(觀光), 사회경제적(社會經濟的) 생산활동(生産活動)을 촉진(促進)하는 사업(事業)을 하게된다. 본(本) 사업(事業)의 공사기간(工事期間)은 1993년(年)부터 5개년간(個年間)이며 총사업비(總事業費)는 외화(外貨) 2천만불(千萬弗)을 포함(包含)하여 5천(千) 5백만불(百萬弗)로 추정(推定)되었다. 년간사업수익(年間事業收益)은 사업(事業)의 완전운영기간(完全運營期間)인 2003년(年)을 기준(基準)으로 할 때 15,541백만불(百萬弗)로 추정(推定)되었으며 사업(事業)의 내구기간(耐久期間)은 30년(年)으로서 2023년(年)까지 생산(生産)이 계속(繼續)될 것이다. 본(本) 사업(事業)의 전체재무수익율(全體財務收益率)은 22.49%이며 경제적(經濟的) 수익률(收益率)은 19.30%로 경제적(經濟的)인 타당성(妥當性)이 매우 높음을 알 수 있다. 모든 사업(事業)이 계획(計劃)대로 성공적(成功的)으로 추진(推進)될 경우 사업지역내(事業地域)의 몽리농가(蒙利農家) 4,000호(戶)가 큰 혜택(惠澤)을 보게되고 농촌(農村)의 빈곤수준(貧困水準)(Poverty Line)은 현재(現在)의 36%에서 18%수준(水準)으로 경감(輕減)될 것이 기대(期待)되고 있다.

• Korean Journal of Heritage: History & Science
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• v.54 no.2
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• pp.194-215
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• 2021

Termites are important decomposers in the ecosystem. They are also economically significant structural pests. In this study, we reviewed the developments of termite control and recent research on termite management to provide information on the prevention and control of termites. In Korea, most of the damage to wooden historical buildings is caused by subterranean termites. Reticulitermes speratus kyushuensis is the main species, which is widely found throughout the country. In the early 1900s, inorganic insecticides, such as arsenic dust, were used for termite control. After the synthesis of chlorinated hydrocarbon pesticide in the 1940s, it was widely utilized and demonstrated high termite control efficacy. However, chlorinated hydrocarbon insecticides were later banned, disappearing from markets after reports emerged concerning environmental contamination and toxicity to humans. Therefore, the termite control industry sought a new termiticide; hence many pesticides were utilized for termite control. Organophosphate (1960s), carbamate (1970s), pyrethroid, and insect growth inhibitor (1980s) were newly synthesized and adopted. In the 1990s, the first commercial baits using chitin synthesis inhibitors (CSI) were developed, providing a means to eliminate an entire colony of subterranean termites around a structure. Many studies have been carried out on soil termiticides (liquid termiticides) and CSI baits to increase their efficacy, and different baits such as aboveground bait stations, fluid bait, and high-durability bait were also developed in the 2000s. In addition, the paradigm of termite control has shifted from localized treatments using soil termiticides to area-wide pest management using CSI baits to create termite-free zones and protect buildings over time. Termite infestations in wooden historical buildings in Korea have been reported since 1980, and considerable attention was drawn in the 1990s when several UNESCO world heritages such as the Jongmyo Shrine and the Janggyeong Panjeon Depositories of Haeinsa Temple were infested by subterranean termites. Since then, a survey of termite infestation in wooden architectural heritage has been conducted, and the National Research Institute of Cultural Heritage and Heritage Care Program regularly monitors those properties. Finally, we suggest termite management using primarily CSI baits, selective application of various soil treatments applied to the object, foundation soil treatment, research and development of durable termite baits, application of area-wide programs for wooden-building complexes, application of integrated termite management (ITM), and regular education for owners and managers to prevent and reduce termite damage.

• Korean Journal of Heritage: History & Science
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• v.56 no.2
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• pp.148-169
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• 2023

Hayeob pigment is known as one of the traditional dark green pigments, but the color, raw material, and manufacturing method have not been clearly identified. However, comparing the analysis results of the particle shape and constituent minerals of Hayeob pigments revealed through pigment analysis studies of colored cultural properties such as Dancheong, Gwaebul, and paintings, Hayeob pigments appear to be the same as Dongrok pigments produced by salt corrosion. Therefore, in order to restore Hayeob pigment, the manufacturing method of Dongrok pigment was studied based on the records of old literature. The Dongrok pigment manufacturing method confirmed in the old literature records is a natural corrosion method in which copper powder and a caustic are mixed and then left in a humid condition to corrode. Based on this, artificial corrosion using a corrosion tester was adopted to corrode the copper powder more efficiently, and an appropriate mixing ratio was selected by analyzing the state of corrosion products according to the mixing ratio of the caustic agent. In addition, the manufacturing method of Dongrok pigment was established by adding a salt removal process to remove residual caustic agents and a purification process to increase chroma during pigment coloring. The prepared Dongrok pigments have a bluish green or green color, show an elliptical particle shape and a form in which small particles are aggregated, and a porous surface is observed. The main constituent elements are copper(Cu) and chlorine(Cl), and the main constituent mineral is identified as atacamite [Cu₂Cl(OH)₃]. As a result of an accelerated weathering test to evaluate the stability of the prepared Dongrok pigments, it was found that the greenness partially decreased and the yellowness significantly increased as deterioration progressed. Before deterioration, the Dongrok pigments had lower yellowness compared to the Hayeob pigments of the old Dancheong, but after deterioration, yellowness increased significantly, and it was found to have a similar chromaticity range as Dancheong's Hayeob pigments. As a result, the prepared Dongrok pigments were confirmed to be similar to Dancheong's Hayeob pigments in terms of color as well as particle shape and constituent minerals.

• Korean Journal of Environmental Agriculture
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• v.22 no.3
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• pp.210-214
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• 2003

Pesticide residues in some vegetables collected at Noeun wholesale market in Daejeon were surveyed and assessed their risk. In 100 samples, the detection rate of pesticide was 46.0% and the rate exceed MRL was 6.0%. Commodities showing high detection rate were lettuce(85.0%), perilla leaf(80.0%) and cucumber(60.0%). Dicarboximide, organochlorine, and azole fungicides and organophosphorus and pyrethroid insecticides were detected. Detection frequency of pesticide was in the order of procymidone, chlorpyrifos, chlorothalonil, cypermethrin and EPN. When the estimated intake amount of the pesticides were compared with ADI to assess their risk, bitertanol, triflumizole and iprobenphos in perilla leaf were higher rate than the other vegetables. However the total amounts of intake of pesticides were estimated to less than 0.46% of ADI.

• Journal of Korean Society of Environmental Engineers
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• v.28 no.6
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• pp.677-686
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• 2006

This study was the effectiveness of two downflow BAF(Biological Aerated Filter) systems at conventional water treatment system. A BAF reactor placed in front of coagulation and sedimentation tanks(Mode A) and after coagulation and sedimentation tanks(Mode B) that were compared in terms of removal of suspended particles, organic matters, and ammonia nitrogen. The suspended particles removal efficiency was over 80% for both Mode A and B, although Mode A gave slightly better results. $BOD_5$ removal and nitrification efficiencies were more than 90% for both reactor. The organic matter and ammonia removals were also superior in the Mode A. The biofilm thickness and biomass increased as increment of EBCT and the upper part of reactor more about 30% than lower part. The specific oxygen uptake rate(SOUR) was higher the upper part of reactor and Mode A than the lower part of reactor and Mode B. A cost analysis showed that the Mode A system was more cost effectiveness. It could save the coagulant dose by about 67% and the chlorine demand by about 95%. The ideal place to put the BAF reactor was in front of the coagulation/sedimentation process.

• Journal of Conservation Science
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• v.35 no.6
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• pp.612-620
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• 2019

A woolen tapestry curtain, owned by the Seoul Museum of Craft Art, is composed of tabby by cotton-warp and wool-weft threads and its patterned part is shown as a tapestry; paint with pigment has been added to it. The chromaticity of this curtain was measured and the substances in the deep red color were confirmed as Hg by an analysis of the ingredients through X-ray fluorescence. This is presumed to be cinnabar or vermilion. Analyses were performed on a total of seven fabric samples, including the warp & weft of the fabric, its trimming, and its back fabric. As a result, the warp of the woolen tapestry curtain was determined to be a cotton fiber with a middle hole or lumen in the cross-section. Furthermore, an infrared peak likewise showed O-H and C-O binding. Wool fibers as wefts were identified with circular and oval cross-sections and IR peaks showed N-H/O-H stretching and amide(-CONH-). The animal hair samples used in the wool fiber are believed to have come from long-tailed goral or goats and the possibility of using easy-to-spin sapsal dog hair is also not to be overlooked. This was determined through a contrast analysis by the Cultural Heritage Administration to identify the animal species used in the tapestry.

• Analytical Science and Technology
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• v.26 no.4
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• pp.235-244
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• 2013

Detection of halogens using laser-induced breakdown spectroscopy (LIBS) in open air is very difficult since their strong atomic emission lines are located in VUV region. In NIR region, there are other emission lines of halogens through electronic transitions between excited states. However, these lines undergo Stark broadening severely. We report the observation of the emission lines of halogens in laser-induced plasma (LIP) spectra in NIR region using a helium gas flow. Particularly, the emission lines of iodine at 804.374 and 905.833 nm from LIPs have been observed for the first time. In the helium ambient gas, Stark broadening of the emission lines and background continuum emission could be suppressed significantly. Variations of the line intensity, plasma temperature, and electron density with the helium flow rate was investigated. Detection of chlorine and bromine in flame retardant of rubbers was demonstrated using this method. Finally, we suggest a pulsed helium gas jet as a practical and ecomonical helium gas source for the LIBS analysis of halogens in open air.

• Journal of Korea Soil Environment Society
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• v.2 no.1
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• pp.13-20
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• 1997

Usage and residue of pesticide were sureyed for five years from three golf courses which have morethan eighteenholes inKangwon-do from 1992 to 1996. A total of 186 samples(soils, lawns, effluents) were collected from these courses and residual levels of four kinds of organochlorines and twelve kinds of organophosphorus pesticides in each samples were determined by GC-ECD and GC-NPD. The amount of pesticide used in each golf course was 1, 635.6kg a year on the average, and that of pesticide to the green and the fairway were 33g/$\textrm{m}^2$ and 3g/$\textrm{m}^2$, respectively. The pesticide which was not permitted for the management of golf course was detected in 24 of 186 samples and its detection rate was 12.9%. The average pesticide residue in soils was 0.208 ppm for captan, 0.031 ppm for daconil, respectively. Detection rate of the prohibited pesticides has decreased every year since 1992, and there were no residues all the tested samples in 1996.Based on these results, it might be judged investigation methods of pesticide residue should be changed with current using pesticide from the prohibited pesticides and the criteria of residual pesticides in effluents of golf course also should be established.

• Korean journal of applied entomology
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• v.33 no.4
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• pp.230-236
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• 1994

Dicofol susceptible two-spotted spider mite(S) which was selected by 150 times of dicofol treatment showed 27 5-fold increase in resistance as compared to S strain. Inheritance of resistance and crossresistance in selected strain($R_d$) were investigated. There were differences oi susceptibility in the dicofol concentmtion-moltaliity relationships between $F_1$ progenies (R♀, XS♂, R♂, XS♀) obtained from reciprocal cross with the S strain and $R_d$, strain. Degree of dominance of the $F_1$ progenies (R♀, XS♂, R♂, XS♀) were -0.29 and -0.72 in adult females and -0.42 and -0.93 in adult males, respectively. These results suggest that inheritance of dicofol resistance is controlled by an incomplete recessive. $R_d$ strain exhibited a high level of cross resistance to amitraz, and also showed cross resistance to pyrethrotd acaricides, acrinathrin and bifenthnn. $R_d$ strain showed non-cross reslstancz to abamectin, chlolfenson, clofentezine, cyhexatm, fenbutatin oxlde, fenpyroximate, hexythiazox, monocrotophos, propargite and tetradifon, but negatively correlated cross-resistance to azocyclotin and fenbutatin oxide belonging to tin acaricides.