• 보건세계
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• v.40 no.12 s.448
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• pp.26-28
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• 1993

세포나 미생물의 특정 DNA 부위를 증폭하고자 할때 이용되는 PCR(polymerase chain reaction, 핵산중합효소 연쇄반응)은 1983년 미국 Cetus사의 Mullos에 의해 고안되었고, 그후 PCR에 의한 마이코 박테리아의 동정 및 검출이 특정 염기서열의 연구가 이루어지면서 활발히 시도되었으며, 아이젠하크 등은 IS6110을 이용하여 본격적으로 PCR이 결핵균 검출에 유용한 도구로 사용될 수 있다는 가능성을 제시하였다. PCR에 의한 DNA증폭은 단시간 내에 이루어진다는 장점 때문에 유전병 및 미생물의 진단에 이용될 뿐 아니라 법의학 분야 및 장기이식에 따른 조직접합 시험에도 이용될 것으로 예상된다. 이에 따라 국내에서도 결핵연구원에서 최초로 일반 임상검사에 적용함으로써 현재 많은 호평을 받고 있는 PCR에 대하여 알아본다

• Proceedings of the Korea Contents Association Conference
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• 2018.05a
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• pp.15-16
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• 2018

NGS 분석과정에서는 정확도를 높이기 위해서 중합 효소 연쇄반응 (PCR)을 통해 DNA 리드들을 증폭한다. PCR 증폭은 DNA 리드의 중복을 발생시키며 중복으로 인해 NGS 과정의 정확성이 저하되는 문제가 발생한다. 본 논문에서는 DNA 리드 중복을 제거하는 도구중 하나인 Samblaster 의 병렬화를 위한 DNA 리드 분할 방법을 제안한다. DNA 리드를 분할하여 Samblaster를 병렬 실행 하도록 하여 수행시간을 단축 할 수 있도록 한다.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2010.05a
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• pp.129-132
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• 2010

The objective of IM rocket motor is to minimize the probability of inadvertent initiation and severity of subsequent collateral damage, hence it is important to define personnel and equipment survivability to a rocket motor accident. The violent response probability associated with shock, impact and thermal effects be minimized. And during production, transportation/storage and stack of rocket motor, sympathetic detonation, giving severe effects of the propagation of adverse reaction on its surroundings, be reduced. Hence Reaction type also based on reaction results of the overpressure, fragment throw and heat flux.

• Journal of the Korean Chemical Society
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• v.36 no.5
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• pp.644-651
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• 1992

The kinetics of the gas phase reaction of ozone(∼0.5 torr) with sulfur trioxide was investigated in the range of 6∼12 torr pressure at 69∼150${\circ}C$. The reaction rate of ozone with sulfur trioxide was faster than the reaction rate of $O_3 in the presence of CO_2 alone. No evidence for a molecular reaction of O_3 with SO_3 was found and the faster rate is probably due to impurity (HX) from the SO_3 reactant which gives rise to a chain reaction initiated by O_3 ＋ HX → OH ＋ O_2 ＋ X and also SO_3 has a larger collision diameter, which may be attributed to the O3 thermal decomposition more feasibly. The proposed experimental law [-d(O_3)/dt] = k_a(SO_3)(O_3) ＋ k_b(O_3)^{3/2} gives a rate constant ka(M-1 s-1) = (1.55 {\pm} 0.67) {\times} 105 e-{(9.27 0{\pm}0.43)kcal/RT}.$

• Restorative Dentistry and Endodontics
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• v.28 no.3
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• pp.241-248
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• 2003

Asaccharolytic Eubacterium균종은 감염 근관에서의 높은 발생 빈도와 독성으로 인해 최근 많이 연구되어지고 있다. 본 연구는 24명의 환자의 감염 근관에서 얻은 22개의 PCR 산물로부터 Eggerthella lenta를 포함한 Eubacterium 균종의 빈도 및 환자의 임상 증상이나 당뇨와의 상관성을 조사한 후 얻은 자료를 토대로 다음과 같은 결과를 얻었다. 1. 22개의 표본 중에서 16개(73%)가 Eubacterium균종을 포함하고 있었으며 이 중 9개의 시편에서 Eubacterium infirmum이 검출되었다. 2. Eggerthella lenta는 어떤 시편에서도 발견되지 않았다. 3, Odds ratio analysis 결과 Eubacterium infirmum은 당뇨병과의 높은 상관성을 보여주었다(OR=9.6, P=0.04).

• Journal of fish pathology
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• v.12 no.1
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• pp.66-69
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• 1999

Occurrences of red sea bream iridovirus disease (RSIVD) in cultured marine fishes were investigated. The infection was detected by the polymerase chain reaction (PCR) used to amplify the red sea bream iridovirus (RSIV). The RSIV infection was widely distributed in fish culture farm around the south coastal area of the Korean peninsula.

• Journal of fish pathology
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• v.1 no.2
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• pp.103-110
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• 1988

For the rapid diagnosis of bacterial diseases of cultured fishes, the immunoperoxidase method was applied to the detection of $\beta$-haemolitic Streptococcus sp. strain KST-2 isolated from tilapia(Oreochromis niloticus). The suitability of field analysis and the sensitivity of the immunoperoxidase method was compared with those of the counterimmunoelectrophoresis(CIE) and the immunodiffusion(ID). Results of testing cross-reactivity which did not indicate any cross reactivity with other fish pathogens, this method was specific to Streptococcus sp. The sensitivity of this method was $1{\times}10^3CFU/ml$ which was at least $10^2$times greater than the CIE and $10^4$times greater than the ID. The immunoperoxidase method was more suitable for field application and more sensitive than other diagnostic techniques tested on this study.

• Polymer(Korea)
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• v.28 no.3
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• pp.218-224
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• 2004

The adhesion and proliferation of mammalian cells on polymeric biomaterials depend on the surface characteristics such as wettability, chemistry, charge and roughness. In order to recognize the correlation between the adhesion and proliferation of human bone marrow derived stem cells (BMSCs) and surface property, radio frequency generated plasma treatment on low density polyethylene (LDPE) has been carried out. The modified LDPE surfaces were characterized by measuring the static water contact angle. The adhesion and proliferation of cells on LDPE films were characterized by cell counting and reverse transcription-polymerase chain reaction (RT-PCR). The water contact angle of the film surface decreased with plasma treatment time. Proto-oncogenes (c-myc, c-fos) and tumor suppressor gene (p153) showed maximum expression with contact angle of 60 ∼ 70$^{\circ}$ range of LDPE film. By cell counting, we confirmed that the rate of cell proliferation appeared the higher on the film surface of the contact angle of 60∼70$^{\circ}$ We concluded that the surface wettability is an important role for the growth and differentiation of BMSCs.

• Journal of fish pathology
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• v.19 no.3
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• pp.267-276
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• 2006

Seventy-five streptococci were isolated from diseased olive flounder, Paralichthys olivaceus in Jeju. Their drug susceptibility and transferable multiple drug resistance were characterized. All isolates were resistant to flumequine (AR) and oxolinic acid (OA) and 26 isolates (34.7%) showed 4～6 multiple resistance of ampicillin (ABPC), AR, doxycycline (DOXY), erythromycin (EM), norfloxacin(NOR), OA and oxytetracycline (OTC) in various combinations. pST9 of a transferable R plasmid was detected from a multiple drug resistance strain, Streptococcus sp., ST9 originated from diseased flounder in Jeju, previously. We performed DNA hybridization to know the distribution of plasmid with the same DNA structure as pST9 in streptococci. Thirteen out of 60 isolates analyzed were positive in colony DNA hybridization and the part of bacteria isolated from raw meal was also hybridized with pST9. It suggested that raw meal is one of the origin of the resistance plasmid and R plasmid with DNA structure differing from pST9 is also involving in multiple drug resistance of the streptococci. In conjugation experiment, we found transferable R plasmid carrying OTC, DOXY and/or EM resistance determinant in the 13 resistance strains. all of the streptococci carrying the transferable R plasmid were similar in RAPD patterns. However, pST -type R plasmid was rare in S. iniae most frequently appearing in flounder farm.

• Korean Journal of Biological Psychiatry
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• v.2 no.2
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• pp.248-251
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• 1995

정신분열병의 약물치료에서 중요한 역할을 하는 도파민의 기전은 지금까지 5종류의 수용체들이 발견되고 클론되면서, 새롭고 보다 근본적인 유전학적 접근을 통해 규명될 수 있게 되었다. 특히, 도파민 수용체 D4 (DRD4)는 막단백질의 세포질쪽 세번째굴곡에 48-bp반복 다형성배열을 가지고있다. 이러한 다형성 반복배열이 신호전달에 참여할 가능성이 높은 막단백질의 세포질쪽 굴곡에 있다는것은, 각 개인의 항정신병약물에 대한 민감성의 차이를 포함하여 정신분열병에 대한 개개인의 유전적 차이를 진단할 수 있는 가능성을 제시한다. 이러한 가능성을 검증하기 위하여 주로 손쉽고 빠른 중합효소연쇄반응(PCR)을 사용하여 DRD4의 아형들을 분류하게 되는데, DRD4의 PCR은 그 반복배열과 그 주변배열의 높은 GC함량(78% G+C) 때문에 일반적인 PCR 방법을 변형시켜 사용해야한다. DRD4의 아형을 분류하기 위해 변형된 PCR은 통상적으로 7-deaza dGTP와 10% DMSO를 사용하게된다. 이러한 DRD4 PCR은 대부분의 경우 성공하지만, 항상 모든 시료에서 PCR이 성공되는것은 아니었으며 반복적으로 시도하여 증폭시킬 수 있었다. 이러한 어려움은 대부분이 template DNA에 문제가 있을것으로 의심되며 DNA정제 또는 template DNA를 제한효소로 적절하게 무작위절단하여 성공율을 높일 수 있었다.