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Biological Control of Plant Pathogen by Pmdornonas sp. (Pseudomondas sp.에 의한 채소병원균의 생물학적 억제)

  • 김교창;김홍수;도대홍;조제민
    • Microbiology and Biotechnology Letters
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    • v.20 no.3
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    • pp.263-270
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    • 1992
  • For the selection of powerful antagonistic bacterium for biological control of soil borne Eminia carotovora subsp. carotovora causing rot of vegetable, excellent strains (S4, S14, 565) were selected from 1,196 strains of bacteria which were isolated from rhizosphere in vegetable root rot-suppresive soil. Strains were identified to be Pseudomonas species with Api 20NE kit. Antagonistic substance was produced in 523 synthetic broth medium at pH 7~8 and $30^{\circ}C$ during 3 days culture. The substance was stable in the pH range of 6 to 9. When the basal medium was supplemented with mannitol and sorbitol as carbon source and calcium chloride as metal salt, the production of the inhibitory substance was increased. The inhibitory acitivity was increased by the addition of fertilizer in soil. The isolated strains were resistant to the agricultural chemical such as benomyl and fosethyl-Al-folpet, and the antibiotics such as penicillin and lincomycin. We had found that Pseudomonas sp. S14 strain had a single plasmid. After treated with acridin orange for curing, we confirmed the existence of antagonistic gene in the chromosomal DNA.

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Development of Antibacterial Using Several Wild Flowers (야생화를 이용한 살균제 개발)

  • Jung, J.H.;Park, N.B.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.21 no.1
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    • pp.71-80
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    • 2019
  • We have been experimenting for industrialize four kinds of wildflowers(Smilax china, Saururus chinensis, Houttuynia cordata and Zizania caduciflora) which are used for Korean medicinal plants for a long time as Korean resources plants. Smilax china, Saururus chinensis, Houttuynia cordata was used for roots and Zizania caduciflora used for leaves. Extraction was carried out in 50% ethyl alcohol, which was concentrated by freezing the material and then injected at a low concentration of 100ppm. The results of the study on the antibacterial potential activity and antifungal potential activity using the four wildflowers strains were as follows. 1. The results of the sterilization and antibacterial potential activity and antifungal potential activity were as follows. Houttuynia cordata showed good inhibitory effect for Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa. The inhibitory power of Zizania caduciflora was proved to some extent. However, other cultivars showed little antibacterial and antifungal activity. 2. The antifungal potential activity against Candida albicans, Aspergillus niger, Aspergillus fumigatus, and Cryptococcus neoformans was small but some inhibition against Candida albicans was confirmed. There was no inhibition of other fungi potential at all.

Natural antibrowning treatments on fresh-cut apple slices (천연 갈변방지제를 이용한 최소가공 사과 절편 개발)

  • Son, Seok-Min
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.8 no.1
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    • pp.151-155
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    • 2007
  • A comprehensive study to evaluate the relative antibrowning activity of 5 known natural juices was conducted. Among the juices tested, rhubarb juice showed the highest inhibitory activity on apple browning (${\Delta}L=0.47$ & ${\Delta}E=1.8$ after 6 storage days at $4^{\circ}C$). Even 50% diluted rhubarb juice was very effective in controlling apple discoloration, showing very limited change in Hunter's L and E values. No significant difference in apple slices browning was observed with respect to the packaging material (LDPE or nylon/PE). However, gas composition inside package was closely related to apple browning.

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Effects of Oil Unsaponifiables and Plant Extracts on the Thermal Oxidation of Oils at $180^{\circ}C$ (유지 불검화물과 식물체 추출물이 식용유의 $180^{\circ}C$ 가열 산화에 미치는 효과)

  • Jung, Mun-Yhung;Yoon, Suk-Hoo;Kim, Seong-Yeol;Lee, Jee-Hyeun
    • Korean Journal of Food Science and Technology
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    • v.29 no.5
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    • pp.860-868
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    • 1997
  • The effects of oil unsaponifiables (8 species) and methanolic plant extracts (53 species) on the thermal oxidation of oils during 14 or 16 hour heating at $180^{\circ}C$ were studied by analyzing the fatty acid composition of soybean oil or cottonseed oil. All the unsaponifiables tested did not show any antioxidative activities on the thermal oxidation of soybean oil. However, among the 53 species of methanolic plant extracts, some species (Rheum undulatum L., Chelidonium majus L. var. asiaticum and Eugennia caryopyllata T.) showed very strong antioxidative activities on the thermal oxidation of cottonseed oil. The antioxidative activities of methanolic extracts of these 3 species on thermal oxidation of cottonseed oil during an extended heating period were also studied by heating cottonseed oils for 7 hours a day for 8 days at $180^{\circ}C$. The methanolic extracts of Chelidonium majus L. v. asiaticum and Eugennia caryopyllata T. greatly reduced both polymer formation and linoleic acid decomposition after the 8 day heating at $180^{\circ}C$. However, the methanolic extracts of Rheum undulatum L. was effective for the reduction of polymer formation, but not for the reduction of linoleic acid decomposition.

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Adsorption and desoption property of activated carbon fiber coated with transition metal (전이금속 코팅 활성탄소섬유의 흡착 및 탈착특성)

  • You, Seung-Han;Kim, Jung-Su;Jang, Hyun-Tae;Cha, Wang-Seog
    • Proceedings of the KAIS Fall Conference
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    • 2011.12a
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    • pp.286-288
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    • 2011
  • 활성탄소섬유의 유해 미생물의 번식 억제력을 조사하기 위해 한천베지배양법을 이용하였다. 전이금속으로 코팅된 활성탄소섬유는 일부 유해 미생물에서 번식억제력이 나타났으며, 유해 미생물은 인간의 피부 부종을 야기시키는 미생물인 candida albicans이었다. 그리고 코팅 활성탄소섬유의 흡 탈착력은 코팅되지 않은 활성탄소섬유보다 약 5% 내외로 감소함을 알 수 있었다.

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Antimicrobial Activities of Viscous Substance from Chongkukjang Fermented with different bacillus spry. (청국장 발효 세균의 종류에 따른 청국장 정절물의 항 미생물 활성에 관한 연구)

  • 윤호경;최희선;허성호;홍정화
    • Journal of Food Hygiene and Safety
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    • v.16 no.3
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    • pp.188-193
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    • 2001
  • To evaluate antimicrobial activities of chongkukjang slime fermented by different strains, growth characteristics were compared using various standard microorganisms with addition of chongkykjang slime. Chonghkjang slime was prepared by fermenting cooked soybean after inoculating with Bacillus circulans K-1, Baciilus spp N-1 and Bacillus subtilis CH-1, respectively. Significant antimicrobial activity was observed by chongkukjang slime on gram positive bacteria (Staphylococcus aureus, Bacillus cereus, Bacillus subtilis, Micrococcus luteus), gram negative bacteria(Escherichia coli O157:H7, Salmonella Typhimurium, Pseudomonas fluorescens), and yeast (Pichia membranaefaciens, Saccharomyces cerevisiae, Candida albicans). In case of B. cereus growth inhibition of 80% was achieved by the addition of chongkukjang slime; on the contrary, to Escherichia coli O157:H7 only 20% inhibition was observed. Slime from Bacillus subtilis CH-1, in particular, inhibition of 40% toward bacteria and yeast, whereas slime from Bacillus circulans K-1, Bacillus spp N-1 showed only 20% inhibition.

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Structure-Activity Relationships of Fungicidal N-Substituted Phenyl 1,3,5- Trimethylpyrazole-4-carboxamides in the Inhibition of Succinate Dehydrogenase (SDH) Isolated from Rhizoctonia solani $K{\ddot{u}}hn$ (벼 잎집무늬 마름병균 (Rhizoctonia solani $K{\ddot{u}}hn$)에서 분리한 Succinate Dehydrogenase (SDH) 에 대한 N-치환 phenyl 1,3,5-trimethylpyrazole-4-carboxamide 유도체의 효소활성저해)

  • Kim, Yong-Whan
    • Applied Biological Chemistry
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    • v.40 no.5
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    • pp.447-450
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    • 1997
  • Eighteen N-substituted phenyl 1, 3, 5-trimethylpyrazole-4-carboxamides were synthesized to screen for their mycelial growth inhibition activity against Rhizoctonia solani $K{\ddot{u}}hn$ $(pEC_{50})$ and to measure enzymatic inhibition activity of these compounds $(pI_{50})$ against succinate dehydrogenase (SDH) isolated from Rhizoctonia solani $K{\ddot{u}}hn$ A structure-activity relationship formulated by regression analysis showed that 79% of the variance in mycelial growth inhibition activity can be explained with SDH inhibition activity and chromatographic capacity factor $(\acute{k})$ as a hydrophobic parameter related to the penetration and transport processes in the biological system.

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Effect of Dropwort (Oenanthe javanica) Extracts on Memory Improvement in Alzheimer's Disease Animal Model, Tg2576 mice (알츠하이머질병 모델동물인 Tg2576 마우스를 이용한 미나리 알코올추출물의 기억력 개선 효능)

  • Won, Beom Young;Shin, Ki Young;Ha, Hyun Jee;Chang, Keun-A;Yun, Yeo Sang;Kim, Ye Ri;Park, Yong Jin;Lee, Hyung Gun
    • Korean Journal of Food Science and Technology
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    • v.47 no.6
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    • pp.779-784
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    • 2015
  • This study was conducted to investigate the effect of herbs on memory improvement by focusing on their cholinergic functions in Tg2576 mice. Seven herbs were used to obtain extracts by using alcohol and water. In screening test for cholinergic activities of the extracts, acetylcholinesterase (AChE) activity was highly inhibited in Oenanthe javanica alcohol extract (OJAE, 18.76%) as compared with the others. The OJAE-treated Tg2576 (Tg-OJAE) groups showed the statistically significant increases of latency time in passive avoidance test. Also, it was found that the concentration of $A{\beta}1-42$ was significantly reduced in Tg-OJAE groups compared to non-treated Tg2576 groups. In the additional enzyme test, it was found that $IC_{50}$ of OJAE was $991.77{\mu}g/mL$ and OJAE acted as an uncompetitive inhibitor of AChE. Therefore, it seemed that OJAE can be used for the development of processed foods for memory improvement.

Influences of Several Chemicals on the Solubility of Cocoon Sericin in Water (제사용수(製絲用水)에 대(對)한 몇가지 화학약제(化學藥劑)의 처리(處理)가 견층(繭層) Sericin의 용해성(溶解性)에 미치는 영향(影響))

  • Rhee, In Jeon;Lee, Dong Soo
    • Current Research on Agriculture and Life Sciences
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    • v.1
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    • pp.55-65
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    • 1983
  • The purpose of this study is to find out influences of several chemicals on the solubility of cocoon sericin in water, and there are several results of use to control the solubility of cocoon sericin in water. The results obtained are summarized as follows : The chemical which shows the strongest accelerating power of the solubility of cocoon sericin in water is Sodium Hydroxide (NaOH), the second is Potassium Hydroxide (KOH), the third is Sodium Silicate ($Na_2SiO_3$), and the weakest is Sodium Bicarbonate ($NaHCO_3$) in order among noticed silk-reeling accelerators. The chemical which shows the strongest inhibiting power of the solubility of cocoon sericin in water is Form Aldehyde (HCHO), the second is Ammonium Alum ($Al_2(SO_4)_2{\cdot}(NH_4)_2SO_4{\cdot}24H_2O$), the third is Acetic Acid ($CH_3COOH$), the weakest is Hydrochloric Acid(HCl) in order among noticed silk-reeling inhibitors. Particulary Hydrochloric Acid (HCl), which is expected to show strong inhibiting power of the solubility of cocoon sericin in water, shows accelerating power of the solubility of cocoon sericin at high temperature over 80 degrees of Celsius thermometer in water.

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