• Journal of Periodontal and Implant Science
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• v.38 no.4
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• pp.679-690
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• 2008

Purpose: The aim of this study was to investigate the effects of EMD on demineralized root surface using human periodontal ligament cells and compare the effects of root conditioning materials(tetracycline(TCN), EDTA). Material and Methods: Dentin slices were prepared from the extracted teeth and demineralized with TCN and EDTA. Demineralized dentin slices were incubated at culture plate with 25, 50 and $100{\mu}g/ml$ concentration of EMD. Cell attachment, alkaline phosphatase activity test, protein synthesis assay and scanning electronic microscopic examination were done. Results: Cells were attached significantly higher in EMD treated group at 7 and 14 days. Cell numbers were significantly higher in EMD treated group. Alkaline phosphatase activity was significantly higher in EMD treated group at 7 and 14 days. Protein synthesis was significantly higher in EMD treated group at 7 and 14 days. Conclusion: Enamel matrix derivatives enhance the biologic activities of human periodontal ligament cells on demineralized root surface and its effects are dependent on the concentration of EMD.

• Journal of Periodontal and Implant Science
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• v.38 no.4
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• pp.729-736
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• 2008

Purpose: Anterior ridge defect after tooth extraction results in unfavorable appearance. Ridge augmentation procedures should be preceded by careful surgical-prosthetic treatment planning, and various techniques can be used in anterior ridge augmentation. Materials and Methods: Three patients showed deformed ridges after tooth extraction. Three different techniques ; onlay-interpositional connective tissue graft; bovine hydroxyapatite graft with free connective tissue graft; bovine hydroxyapatite graft with resorbable collagen membrane following free connective tissue graft; were used for anterior ridge augmentation. Result: Soft tissue graft can be used in small amount of ridge defect, hard tissue graft combined with soft tissue graft can be used in large amount of ridge defect. After ridge augmentation, about three months of healing period, augmented tissue was stabilized. The final restoration was initiated after this healing period, and the tissue form was maintained stable. Conclusion: Careful diagnosis and surgical-prosthetic treatment planning with joint consultation prior to surgery should be performed in order to attain an optimal esthetic results.

• Journal of Periodontal and Implant Science
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• v.38 no.4
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• pp.621-628
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• 2008

Purpose: Osteopontin is one of the major non-collagenous protein of hard tissue. Use of peptide domain of biologically active protein has some advantages. The objective of this experimental study is evaluation of periodontal regenerative potency of synthetic peptide gel which containing collagen binding domain of osteopontin in the degree III periodontal defect of beagle dogs. Material and Methods: Experimental degree III furcation defect was made in the mandibular third and fourth premolar of beagles. Regenerative material was applied during flap operation. 8 weeks after regenerative surgery, all animals were sacrificed and histomorphometric measurement was performed to calculate the linear percentage of the new cementum formation and the volume percentage of new bone formation. Result: The linear percent of new cementum formation was 41.6% at control group and 67.1% at test group and there was statistically significant difference. The volume percent of new bone formation was 52.1% at control group and 58.9% at test group. Conclusion: As the results of present experiment, synthetic peptide gel containing collagen binding domain of osteopontin significantly increase new bone and cementum formation in the degree III furcation defect of canine mandible.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.15 no.1
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• pp.17-22
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• 1979

To catch live anchovies which will be used as bait for skipjack fishing, set net, lift net and purse seine net were tried in Korean coastal waters by the authors. Among them the former two methods showed unsuitable to catch in bulk even though the catch showed good survival. The last showed unsuitable result because of the lack of exclusive fishing boat and skilled fishermen. From the viewpoint of practical use, the authors regarded it may be suitable to improve the traditional boat seine net which are used as major fishing gear to catch anchovies in Korea, so far as Korea is concerned. So we tried to catch live anchovies with partially reconstructed boat seine net and to hold the catch in the creel improved by the authors. Even though the result showed less than 70 percent of survival, slightly worse than other fishing methods, the experimented method showed to be practical in catching the live anchovies intensively.

• The Korean Journal of Food And Nutrition
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• v.22 no.4
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• pp.644-649
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• 2009

This study was conducted to determine the influence of cultural conditions such as temperature, time, water content, koji-thickness and agitation on the production of saccharifying amylase by Rhizopus sp. ZB9 isolated from Korean Nuruk during the preparation of rice koji, which is used in brewing Korean rice wines, Takju and Yakju. Rice kojies were made under different cultural conditions, and the saccharifying activities of each koji were tested. The temperature range suitable for the production of saccharifying amylase was $28{\sim}36^{\circ}C$. Based on the saccharifying activity and color, 60 hours of cultivation at $28^{\circ}C$ was believed to produce the optimum results. The water contents of steamed rice suitable for the production of saccharifying amylase were 35~40%. An increase in koji-thickness induced no adverse effects on the production of saccharifying amylase, but agitation-work during cultivation had a harmful effect.

• 한국해양학회지
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• v.12 no.2
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• pp.57-66
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• 1977

Chlorophyll concentrations of phytoplankton were determined by 5 meters interval from surface to bottom layers in Yeongil Bay water of Korea, late October 1973. The contents of phytoplankton chlorophyll decreased outward from the inner part to the entrance of the Bay. It seemed to be caused by incoming land waters which are rich in nutrients. And vertical distribution pattern of the chlorophyll showed three layer zones in the middle and outer parts, and two layers in the inner part of the Bay. These phenomena suggest that there are two different water masses, the one is bottom cold water originated from offshore, the other is surface warm water. And they are mixed together in the middle layer The correlationship between the contents of chlorophyll a and chlorophyll c represented perfect positive relation with coefficient of r 0.999 from the extracted data. In general, chlorophyll a percent-content of total chlorophyll varied in the range of 14.64∼19.83% (mean value; 18.74%) and chlorophyll c content was about as much as four times compared with chlorophyll a content.

• Applied Biological Chemistry
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• v.30 no.3
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• pp.264-271
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• 1987

Baikhaju, Nockpaju, Dongdongju and Chungmyungju of the traditional Yakjus - the native alcoholic beverage of Korea - with the different fermentation methods were brewed and the volatile odor components were analized by gas chromatography methods of headspace gas. The general constituent content in them was different a little, and formaldehyde, acetone, acetaldehyde, ethylacetate, ethylalcohol, n-propanol, iso-butanol, iso-amylalcohol, diacetyl and dimethyl sulfide were separated and quantified into the volatile aroma components. The contents of acetaldehyde, ethylacetate and n-propanol were $17{\sim}73ppm$, and iso-amylalcohol were $418{\sim}925ppm$. The contents of formaldehyde and acetone were $0{\sim}2.15ppm$, and that of diacetyl and dimethyl sulfide were $0.032{\sim}1.012ppm$, and $5{\sim}65ppb$.

• Microbiology and Biotechnology Letters
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• v.26 no.5
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• pp.450-455
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• 1998

Aspergillus oryzae M-2-3 strain (argB$\^$-/) was transformed with pTAalp plasmid which was constructed for expression of the alkaline pretense gene, alpA, and 16 transformants were selected on arginine minus medium. When these transformants were tested for productivity of alkaline proteases using agar plate containing skim milk, the halo was observed around each colony of transformants, but not observed around the host strain in this condition. Southern analysis showed that the pTAalp plasmid having alpA gene was integrated into the chromosome of the host strain. The highest level of alkaline protease production was obtained in the culture filtrate of the transformant No. 14, which was estimated to 80-90% of total secreted proteins, and the enzyme activity was 64-450 times higher than those of host strain and industrial strain. Total nitrogen content and the digestion rate in soybean Koji extracts were also increased to 1.5 times in Aspergillus oryzae transformant No. 14.

• Journal of Biosystems Engineering
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• v.7 no.2
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• pp.1-7
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• 1983

In order to identify the stress distribution on the furrow slice a small soil bin instrumented with soil stress meters was designed and constructed. From a series of experiments conducted in the soil bin the following results were obtained. 1) Neither the cutting conditions nor the soil conditions affected the direction of the principal stress. 2) The magnitude of the principal stress increased as the tillage depth increased. However, no effects due to lift angles were shown on the magnitude of the principal stresses. 3) The maximum principal stress increased with increase of the moisture and clay contents in the soil. 4) In the clay soil, the maximum principal stresses were distributed uniformly over the tillage depth. However, as the sand content increased, the maximum principal stresses decreased gradually on the top layer so that the distribution over the tillage depth became a trapezoidal shape.

• Journal of Korean Medical classics
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• v.31 no.1
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• pp.1-14
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• 2018

Objectives : This paper reviews the possibility of confusing wind-cold and cold dryness that can be found in the critical discussion revolving Wu Jutong's concept of cold dryness. With this, the paper aims to refresh the understanding of the concept of cold dryness as put together by Wu Jutong. Methods : The paper reviews the concept of Wu Jutong's cold dryness based on today's critical discussions on the topic. At the same time, the paper searches for additional theoretical basis that can be proposed with regards to Wu Jutong's concept of cold dryness. Results : The core element of the criticisms against Wu Jutong's cold dryness is that cold dryness's cold dryness pattern proposes cold fluid as one of the main symptoms, and that Xiaoqinglongtang is referred to as a cure. However, the problem of the dryness and dampness that can be found in Wu Jutong's cold dryness seems that it can be explained by the discussions regarding Shenkuzao as written in Neijing. Moreover, considering the fact that the connection between cold paathogen and Shenkuzao that surfaced in historical medical theories can also be applied to the concept of cold dryness, it seems that it can be helpful in understanding the characteristics of disease mechanism of cold dryness. Conclusions : The discussion on the concept of cold dryness originally derives as a branch of the discussion on cold. Acknowledging this, it seems that reconsideration is required on the assertion that Wu Jutong confused the symptoms of wind-cold with that of cold dryness.