• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.2
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• pp.1200-1206
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• 2015

The major target for drug discovery, G-protein coupled receptor (GPCR) is involved in many physiological activities and related to various diseases and disorders. Among experimental techniques relating to the GPCR drug discovery process, various cell-based screening methods are influenced by cell conditions used in the overall process. Recently, the utilization of frozen cells is suggested in terms of reducing data variation and cost-effectiveness. The aim of this study is to evaluate various conditions in cell freezing such as temperature conditions and storage terms. The stable cell lines for calcium sensing receptor and urotensin receptor were established followed by storing cultured cells at $-80^{\circ}C$ up to 4 weeks. To compare with cell stored at liquid nitrogen, agonist and antagonist responses were recorded based on the luminescence detection by the calcium induced photoprotein activation. Cell signals were reduced as the storage period was increased without the changes in $EC_{50}$ and $IC_{50}$ values $EC_{50}:3.46{\pm}1.36mM$, $IC_{50}:0.49{\pm}0.15{\mu}M$). In case of cells stored in liquid nitrogen, cell responses were decreased comparing to those in live cells, however changes by storage periods and significant variations of $EC_{50}/IC_{50}$ values were not detected. The decrease of cell signals in various frozen cells may be due to the increase of cell damages. From these results, the best way for a long-term cryopreservation is the use of liquid nitrogen condition, and for the purpose of short-term storage within a month, $-80^{\circ}C$ storage condition can be possible to adopt. As a conclusion, the active implementation of frozen cells may contribute to decrease variations of experimental data during the initial cell-based screening process.

• Journal of the Microelectronics and Packaging Society
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• v.22 no.3
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• pp.31-38
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• 2015

In order to achieve a high speed and high quality silicon wafer bonding, the room-temperature direct bonding using atmospheric pressure plasma and sprayed water vapor was developed. Effects of different plasma fabrication parameters, such as flow rate of $N_2$ gas, flow rate of CDA (clear dry air), gap between the plasma head and wafer surface, and plasma applied voltage, on plasma activation were investigated using the measurements of the contact angle. Influences of the annealing temperature and the annealing time on bonding strength were also investigated. The bonding strength of the bonded wafers was measured using a crack opening method. The optimized condition for the highest bonding strength was an annealing temperature of $400^{\circ}C$ and an annealing time of 2 hours. For the plasma activation conditions, the highest bonding strength was achieved at the plasma scan speed of 30 mm/sec and the number of plasma treatment of 4 times. After optimization of the plasma activation conditions and annealing conditions, the direct bonding of the silicon wafers was performed. The infrared transmission image and the cross sectional image of bonded interface indicated that there is no void and defects on the bonded wafers. The bonded wafer exhibited a bonding strength of average $2.3J/m^2$.

• Journal of the Institute of Electronics and Information Engineers
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• v.53 no.11
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• pp.32-40
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• 2016

IEEE 802.15.4 is the one of the protocols for radio communication in a personal area network. Because of low cost and low power communication for IoT communication, it requires the highest optimization level in the implementation. Recently, the studies of location aware algorithm based on IEEE802.15.4 standard has been achieved. Location estimation is performed basically in equal consideration of reference node information and blind node information. However, an error is not calculated in this algorithm despite the fact that the coordinates of the estimated location of the blind node include an error. In this paper, we enhanced a conventual maximum likelihood estimation using weighted coefficient and implement the hardwired location aware engine for small code size and low power consumption. On the field test using test-beds, the suggested hardware based location awareness method results better accuracy by 10 percents and reduces both calculation and memory access by 30 percents, which improves the systems power consumption.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.12
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• pp.8357-8362
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• 2015

The emergency call device has been installed and operated for the elevator passenger safety, but the call can not be connected or delay to the operator in an emergency. The application of the Internet of Things(IoT) technology is required for the systematic management and reliability of the emergency call device installed in the elevator. To meet these requirements it is necessary to apply the RMS(Remote Management System). Emergency call device periodically reports the operating status information and the emergency call history information of the emergency call device to the RMS in the PSTN(Public Switched Telephone Network) environment, and transmits the call request information in an emergency. RAS(Remote Access Server) plays a role of converting the PSTN signals to TCP / IP. RMS reports a significant outbreak call and abnormal operating conditions to administrators. In this study, we developed optimized protocol to PSTN environment that is required by RMS and implemented its differentiated process. And a RAS-RMS Protocol was added to the Application Layer. Development protocol consisting of simple structure as compared to CoAP, MQTT or HTTP can work properly in low-spec CPU and transmit elevator emergency key information easily.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.17 no.6 s.109
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• pp.562-573
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• 2006

It is required to reduce the peak-to-average power ratio(PAPR) in an orthogonal frequency division multiplexing system or a multicarrier system. And it is needed to eliminate the transmission of the side information in the Partial Transmit Sequences. So, in this paper, a new technique is proposed, where the subcarriers used for the multiple signal representation are only utilized for the reduction of PAPR to eliminate the burden of transmitting the side information. That is, it is proposed by taking the modified minimization criteria of partial transmit sequences scheme instead of using the convex optimization or the fast algorithm of tone reservation(TR) technique As the result of simulation, the PAPR reduction capability of the proposed method is improved by 3.2 dB dB, 3.4 dB, 3.6 dB with M=2, 4, 8(M is the number of partition in the so-called partial transmit reduction sequences(PTRS)), when the iteration number of fast algorithm of TR is 10 and the data rate loss is 5 %. But it is degraded in the capability of PAPR reduction by 3.4 dB, 3.1 dB, 2.2 dB, comparing to the TR when the data rate loss is 20 %. Therefore, the proposed method is outperformed the TR technique with respect to the complexity and PAPR reduction capability when M=2.

• Journal of Broadcast Engineering
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• v.9 no.1
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• pp.17-31
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• 2004

This paper proposes an algorithm for the robust transmission of video in error Prone environment using multiple description codingby optimal split of DCT coefficients and rate-distortionoptimization framework. In MDC, a source signal is split Into several coded streams, which is called descriptions, and each description is transmitted to the decoder through different channel. Between descriptions, structured correlations are introduced at the encoder, and the decoder exploits this correlation to reconstruct the original signal even if some descriptions are missing. It has been shown that the MDC is more resilient than the singe description coding(SDC) against severe packet loss ratecondition. But the excessive redundancy in MDC, i.e., the correlation between the descriptions, degrades the RD performance under low PLR condition. To overcome this Problem of MDC, we propose a hybrid MDC method that controls the SDC/MDC switching according to channel condition. For example, the SDC is used for coding efficiency at low PLR condition and the MDC is used for the error resilience at high PLR condition. To control the SDC/MDC switching in the optimal way, RD optimization framework are used. Lagrange optimization technique minimizes the RD-based cost function, D+M, where R is the actually coded bit rate and D is the estimated distortion. The recursive optimal pet-pixel estimatetechnique is adopted to estimate accurate the decoder distortion. Experimental results show that the proposed optimal split of DCT coefficients and SD/MD switching algorithm is more effective than the conventional MU algorithms in low PLR conditions as well as In high PLR condition.

• Journal of Broadcast Engineering
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• v.12 no.4
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• pp.360-372
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• 2007

We propose a fast inter-layer mode decision method by utilizing coding information of base layer upward its enhancement layer inscalable video coding (SVC), also called MPEG-4 part 10 Advanced Video Coding Amendment 3 or H.264 Scalable Extension (SE) which is being standardized. In this paper, when the motion vectors from the base layer have zero motion (0, 0) in inter-layer motion prediction or the Integer Transform coefficients of the residual between current MB and the motion compensated MB by the predicted motion vectors from the base layer are all zero, the block mode of the corresponding block to be encoded at the enhancement layer is determined to be the $16{\times}16$ mode. In addition, if the predicted mode of the MB to be encoded at the enhancement layer is not equal to the $16{\times}16$ mode, then the rate-distortion optimization is only performed on the reduced candidated modes which are same or smaller partitioned modes. Our proposed method exhibits the complexity reduction in encoding time up to 72%. Nevertheless, it shows negligible PSNR degradation and bit rate increase up to 0.25dB and 1.73%, respectively.

• Journal of Advanced Marine Engineering and Technology
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• v.37 no.4
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• pp.409-417
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• 2013

Mu-communication board supported by multi-communication is designed with Atxmega 128A1 which is a low power energy consuming of 8-bit microcontroller. ATxmega128A1 microcontroller consists of 8 UART(Universal asynchronous receiver/transmitter) ports which can be setting appropriate user interface having command line interpreter(CLI) program with each port, 2 kbytes EEPROM, 128 kbytes flash memory, 8 kbytes SRAM. 8 URAT ports are used for the multi communication modem, GPS module, etc. and EEPROM is used for saving a configuration for program running, and flash memory of 128 kbytes is used for storing a Firm Ware, and 8 kbytes SRAM is used for stack, storing memory of global variables while program running. If we uses the hybrid communication of path optimization of VHF, TRS and CDMA to remote control AtoN(aid to navigation), it is able to remove the communication shadow area. Even though there is a shadow area for individual communication method, we can select an optimum communication method. The compatibility of data has been enhanced as using of same data frame per communication devices. For the test, 8640 of data has been collected from the each buoy during 30 days in every 5 minutes and the receiving rate of the data has shown more than 99.4 %.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.45 no.1
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• pp.59-65
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• 2008

In this paper, a new adaptive resource allocation scheme is proposed in orthogonal frequency-division multiple access(OFDMA) systems with rate proportionality constraints. The problem of maximizing the overall system capacity with constraints on bit error rate, total transmission power and rate-proportionality for user requiring different classes of service is formulated. Since the optimal solution to the constrained fairness problem is extremely complex to obtain, a low-complexity suboptimal algorithm that separates subchannel allocation and power allocation is proposed. Firstly, the number of subchannels to be assigned to each user is determined based on the users' average signal-to-noise ratio and rate-proportion. Subchannels are subsequently distributed according to the modified max-min criterion. Lastly, based on the subchannel allocation, the optimal power allocation by solving the Language dual problem is proposed. Additionally, in order to reduce the computational complexity, iterative rate proportionality tracking algorithm is proposed for maximizing the capacity together with maintaining the rate proportionality constraint.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.39 no.5
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• pp.8-15
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• 2002

We optimally designed the VCO(voltage-controlled oscillator) with spiral inductor using the MOSIS HP 0.5${\mu}{\textrm}{m}$ CMOS process. With the developed SPICE model of spiral inductor, the quality factor of spiral inductor was maximized at the operating frequency by varying the layout parameters, e.g., metal width, number of turns, radius, space of the metal lines. For the operation frequency of 2㎓, the inductance of about 3nH, and the MOSIS HP 0.5 CMOS process with the metal thickness of 0.8${\mu}{\textrm}{m}$, oxide thickness of 3${\mu}{\textrm}{m}$, the optimal width of metal lines is about 20${\mu}{\textrm}{m}$ for the maximum Quality factor. With the optimized spiral inductor, the VCO with LC tuning tank was designed, fabricated and measured. The measurements were peformed on-wafer using the HP8593E spectrum analyzer. The oscillation frequency was about 1.610Hz, the frequency variation of 250MHz(15%) with control voltage of 0V - 2V, and the phase noise of -108.4㏈c(@600KHz) from output spectrum.