• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.04a
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• pp.181-181
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• 1994

포유 동물의 중추 신경계에서 주 억제성 신경전달물질로 알려진 4-aminobutyrate(GABA) 대사에 관여하는 효소인 GABA transaminase (GhBA-T)와 Succinic semialdehyde reductase(SSR)를 각각 여러 가지 크로마토그래픽 방법을 사용하여 순수 분리 정제하였다 GABA-T는 subunit 분자량 50kDa을 가진 dimer이며 화학 변형(chemical modification), 흡광, 형광스펙트럼 연구에 의하면 cysteinyl잔기가 활성 부위에 존재하고 있음을 보여주었다 SSR은 2-mercaptoethanol이 존재하든 안하든 o-pthalaldehyde(OPA)에 의하여 효소 활성도가 상실되었으며 흡광 및 형광스펙트럼에 의하면 OPA가 효소의 활성 부위의 lysyl잔기와 반응하여 cysteinyl잔기와 함께 intermolecular cross-linking을 이루는 것을 알 수 있었다. 한편 SSR은 몇가지 anticonvulsant drug에 의하여 부분적인 저해 효과를 나타내었다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.131-131
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• 2018

중국(中國) 장홍화(藏紅花)는 해발 1500m~3000m에 자생하는 고원식물이다. 장홍화는 여성질환예방과 두뇌의 신경안정, 호르몬분비 촉진, 학습증진과 기억력향상에 의한 알츠하이머 치료의 효과가 있는 것으로 알려져 있으며, 함유되어 있는 크로신은 암세포를 파괴시키어 종양세포를 줄이는 작용을 하며, 카로티노이드 성분이 항산화 활성과 면역을 증진시키어 항암효과를 가져다 준다. 또한 다량의 칼륨을 함유하고 있어서 나트륨배출에 효과적이어서 혈압을 떨어뜨리는 작용을 한다. 또한 신경전달물질의 활동을 증진시키어서 스트레스 해소와 치매 및 인지력을 높여준다고도 알려져 있다. 본 실험에서는 장홍화(藏紅花)추출액의 총 폴리페놀 함량과 항산화 물질 활성을 조사했던 바, 장홍화(藏紅花) 메탄올과 에탄올추출액에서 항산화 반응이 매우 양호하였다. 또한 총 폴리페놀 함량도 에탄올 추출물과 메탄올 추출물 처리구에서도 비슷한 결과를 나타내었다. 이러한 결과들로 미루어 볼 때 장홍화(藏紅花)의 추출액은 건강음료와 화장품의 기능성원료로도 이용이 가능할 것으로 추측되었다.

• Microbiology and Biotechnology Letters
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• v.28 no.2
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• pp.97-104
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• 2000

Screening of Biologically Active Essential Oils from Ligusticum tenuissimum. Kim, Min-Hae, Young-Gil Kim, Jin-Ha Lee, Keo-Pyo Hong, Jung-Ki Hong, Young-Joon Kong, and Hyeon-Yong Lee*. Division of Food and Biotechnology, Kangwon National University, Chunchon 200-701, Korea, 1 Regional Crop Development Station, Kangwon Agricultural Research & Extension Services, Chunchon 200-150, Korea-The biological activities of the crude essential oils from Ligusticum tenuissimum and the control(phthalic anhydride) were compared. About 60% of the growth of MCF7, A549, and Rep3B cells were inhibited by adding 1.0 mg/ml of the crude essential oils and below 40% was observed by the control. Cytotoxicity on human normal lung cell(IMR90) was scored as 34.4% for the crude oil and 26.4% for control, respectively. It was found that the crude essential oils were more effective than the control in anti mutagenecity tested by both Rec-assay and CRG V79 cells. The growth of human T-cell(Jurkat) was enhanced up to 1.21 times by adding the crude essential oil compared with the control. 50% of a-glucosidase activity was inhibited by both the crude essential oil and the control. ACE activities were inhibited 80.1 % and 65.3% by adding 1.0 mg/ml of the crude oil and the control, respectively. The higher enhancement of glutathione-S-transferase activity was observed in the crude oil than those in the control: 301 % v.s 234% at 1.0 mg/ml of the treatment. Thrombolytic activity was measured as 42.9% and 28.6% for the crude oil and the standard, respectively. The effect of the oil on the nerve cells PCI2, was observed as follows: the neurite of PCl2 cells was lengthened up to 255 /-lm longer than 205 /-lm of control. The number of neurite-bearing cells were about two times higher than control. The survival ratio of the crude essential oil was also increased up to 56.4% which was about two fold higher than in control.

• Journal of Convergence for Information Technology
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• v.10 no.9
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• pp.200-205
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• 2020

This study investigates the effects of instrument-assisted soft mobilization (IASTM) and manual myofascial release (MFR) on the muscle activity of the biceps brachii. This study was conducted on 10 men and women in their 20s, and all subjects participated in the experiment for 3 days and measured muscle activity of the upper forearm muscles. On the first day, the muscle activity value was measured before the intervention, and the remaining two days were measured for muscle activity after the intervention of each manual therapy in a random order. All muscle activity values were compared and analyzed through the dependent t test and the independent t test. The before and after comparison of muscle activity values before and after each intervention was verified by performing the dependent t test and comparing the values of muscle activity between groups between each intervention after intervention by performing an independent t test. According to the results of this study, both interventions significantly increased muscle activity of the biceps brachii before and after intervention, and there was no significant difference in muscle activity values between groups after intervention. Therefore, both manual therapy (IASTM, MFR) are thought to be effective in improving neuromuscular control ability.

• Journal of Biomedical Engineering Research
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• v.21 no.5
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• pp.487-494
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• 2000

본 논문에서는 배경잡음이 섞여 있는 QRS 파의 증대를 위해 신경망에 근거한 적응라인증대기(ALE) 적용을 다루고 있다. Elman과 Jordan RNN 구조의 합성형태를 갖는 수정된 완전연결 리커런트 신경망이 ALE의 비션형 적응필터로 사용되고 있다. 신경망 노드사이의 연결계수와 이득, 기울기, 지연과 같은 노드 활성함수의 변수들이 기울기 강하 알고리즘을 사용하여 학습이 반복될 때마다 갱신된다. 수정된 신경망은 먼저 미지의 선형과 비선형 시스템 identification을 수행함으로써 평가하였다. 그리고 미약한 QRS를 증대시키기 위해서 적당한 크기의 잡음과 매우 심한 잡음이 포함된 실제의 ECG 신호를 비선형 신경망 적응필처를 사용하는 ALE에 입력하였다. 수정된 신경망은 시스템 identification에 사용하기가 적합함을 확인하였으며, 시뮬레이션 결과에 의하면 신경망 ALE는 잡음 ECG 신호로부터 QRS 파를 증대를 잘 수행하였다.

• Journal of the Korean Applied Science and Technology
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• v.36 no.4
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• pp.1303-1311
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• 2019

This study was to investigate the antioxidant, anti-inflammatory and neuronal cell protective effects of Poria cocos Wolf and Corni fructus extracted by water and 70% ethanol. Total polyphenol content in water extract of Poria cocos Wolf was significantly higher than those of other extracts. DPPH and ABTS free radical scavenging activity in water extract of Corni fructus was higher than those of other extracts. DPPH and ABTS free radical scavenging activity were increased in a dose-dependent manners. In order to effectively extract total polyphenol contents and anti-oxidant components in Poria cocos Wolf and Corni fructus, hot water extraction method is more efficient than ethanol extraction method. Poria cocos extracts were found to be a superior NO production inhibitory effect compared to Corni fructus extracts. In a neuronal cell viability assay using MPP⁺, the water extract of Poria cocos Wolf protected against MPP⁺-induced neurotoxicity than those of Corni fructus extract. It is considered to be a potential functional material with antioxidant, anti-inflammatory and neuronal protective effect against to oxidative stress according to the extract methods of extracting Poria cocos Wolf and Corni fructus.

• Applied Microscopy
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• v.29 no.4
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• pp.459-470
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• 1999

To investigate the changes during the differentiation of the cerebral neurons of the embryogenic day (ED) 9 and 10, investigated the ultrastructural changes in the cerebral neurons by Electromicroscope, also cerebral protein, the activity of dehydronases (LDH, MDH and SDH) and changes of adenosine triphosphate concentration were analyzed, the result obtained are as follows. In the ultrastructural changes in the cerebral neurons, chromatin in 9 day-old chick embryos are comparatively distributed to even in neucleoplasm and could investigate very prominently that nuclear membrane is double-layer. Esperially, Rough endoplasmic reticulum (RER) and Golgi complex are developed well, also polysome is investigated and synaptic vesicles were scattered. In 10 day-old chick embryos, chromatin evenly spread and nuclear membrane could be differentiated prominently. Rough endoplasmic reticulum (RER) contain cytoplasm, mitochondria and Golgi complex are comparatively developed well. In 9 day-old cultural group of chick embryo cerebrum were separated 37 polypeptide bands and In 10 day-old cultural group of chick embryo cerebrum were separated 38 poly -peptide bands. The more culture time increase, the more the activity of dehydronases (LDH, MDH and SDH) increase. LDH activity was 11.07 (9th day) and 12.12 (10th day), MDH activity was 11.89 (9th day) and 13.44 (10th day) and SDH activity was 8.45 (9th day) and 10.52 (10th day) respectively. The ATP concentration degreesed 10 day-old cultural group than 9 day-old cultural group.

• The korean journal of orthodontics
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• v.34 no.6 s.107
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• pp.506-513
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• 2004

Osteoclast action is necessary for alveolar bone remodeling in orthodontic tooth movement. The nervous system has also been reported to be associated with bone remodeling. This study was aimed to investigate the changes of osteoclasts in the periodontal ligament (PDL) space after surgical resection of the inferior alveolar nerve (IAN). Experimental rats were divided into young and adult groups. A surgical resection procedure of the IAN was carried out in the left side of the mandible and a sham operation in the right side of the mandible. The number of osteoclasts on the bundle bone surface and the resorption activity of the osteoclasts were histomorphometrically measured. The changes in distribution of substance P (SP) immunoreactive (IR) nerve fiber were evaluated in the PDL and pulp. SP-IR nerve fiber was depleted in both the PDL and pulp of the IAN resection side in both groups, which confirmed the resection of IAN to be successfully conducted. The number of osteoclasts in the IAN resection side was significantly reduced in both the young and adult groups (p<0.01 and p<0.05), whereas the resorption activity of osteoclasts did not show any significant difference between the IAN resection side and the sham operation side in both groups (p>0.05 and p<0.05). The adult group showed that the number of osteoclasts reduced significantly (p<0.01) and the resorption activity didn't change in comparison with the young group (p>0.05). These results suggest that surgical resection of the IAN and aging reduce the population of the recruited osteoclasts within the PDL, but don't affect on the osteoclastic resorption activity.

• Journal of Life Science
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• v.26 no.6
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• pp.640-645
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• 2016

Microglial cells are immediately activated in the central nervous system in response to a variety of neuronal environmental changes, such as injuries or inflammation. In addition to the modulation of the intrinsic immune response, a key role of microglial cells is the phagocytosis of dying cells and cellular debris. In this study, the inhibitory effects of epigallocatechine-3-gallate (EGCG), a most abundant and active polyphenol component of green tea, on lipopolysaccharide (LPS)-induced microglial activation are determined. EGCG dose dependently suppressed LPS-induced nitric oxide production and the expression of inducible nitric oxide synthase (iNOS) in BV-2 microglial cells. EGCG are potent LPS-induced inhibitors of several pro-inflammatory cytokine expressions, such as TNF-α and IL-1β, in microglial cells. Furthermore, EGCG generally inhibits the induction of LPS-mediated microglial activation and potently inhibits the phagocytosis of LPS-stimulated BV2 microglia. Although the conditioned media from LPS-stimulated BV-2 cells caused the SN4741 cell death, that from the conditioned media of EGCG pretreated BV-2 cells did not diminish the viability of SN4741 cells. These results suggest EGCG, a green tea polyphenol, could be a promising available molecule for the modulation of harmful microglial activation.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.8 no.1
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• pp.65-69
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• 2015

Joint flexibility is an important factor which affect the process and duration of the therapeutic methods in the filed of occupational therapy. Hip joint flexibility and electromyography (EMG) of major flexor and extensor for the hip joint were examined to understand the biomechanical characteristics of Proprioceptive Neuromuscular Facilitation (PNF). Hip joint flexibility increased $18.9^{\circ}$ on average after PNF was performed by a designated assistant on 10 college students. EMGs of quadriceps femoris muscle and hamstring muscles agreed with biomechanical characteristics of proprioceptive organs in muscles.