Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
권호 표지
DOI QR코드

DOI QR Code

Biological Activities of Poria cocos Wolf and Corni fructus Extracts based on their extraction solvent

추출용매에 따른 복령(Poria cocos Wolf)과 산수유(Corni fructus) 추출물의 생리활성 효과 비교

  • 오희경 (장안대학교 건강과학부 바이오동물보호과)
  • Received : 2019.12.07
  • Accepted : 2019.12.30
  • Published : 2019.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study was to investigate the antioxidant, anti-inflammatory and neuronal cell protective effects of Poria cocos Wolf and Corni fructus extracted by water and 70% ethanol. Total polyphenol content in water extract of Poria cocos Wolf was significantly higher than those of other extracts. DPPH and ABTS free radical scavenging activity in water extract of Corni fructus was higher than those of other extracts. DPPH and ABTS free radical scavenging activity were increased in a dose-dependent manners. In order to effectively extract total polyphenol contents and anti-oxidant components in Poria cocos Wolf and Corni fructus, hot water extraction method is more efficient than ethanol extraction method. Poria cocos extracts were found to be a superior NO production inhibitory effect compared to Corni fructus extracts. In a neuronal cell viability assay using MPP+, the water extract of Poria cocos Wolf protected against MPP+-induced neurotoxicity than those of Corni fructus extract. It is considered to be a potential functional material with antioxidant, anti-inflammatory and neuronal protective effect against to oxidative stress according to the extract methods of extracting Poria cocos Wolf and Corni fructus.

본 연구는 열수와 에탄올 용매에 따른 복령 및 산수유 추출물의 농도별로 항산화 활성, 항염증 효과 및 신경세포 보호효과에 미치는 영향을 살펴보고자 진행하였다. 추출물들의 총 polyphenol 함량은 복령 열수 추출물에서 가장 높았고, 복령 에탄올 추출물, 산수유 열수 추출물, 산수유 에탄올 추출물 순으로 유의적으로 감소되는 경향을 나타냈다. DPPH 및 ABTS radical 소거 활성능은 추출물의 농도 의존적으로 증가하였고 산수유 열수 추출물은 모든 추출물에서 항산화 활성이 가장 높게 나타났으며, 복령 열수 추출물은 에탄올 추출물에 비해 높은 활성이 확인되었다. 복령과 산수유에 함유된 총 polyphenol과 항산화 성분을 추출하기 위해서는 에탄올 추출방법 보다는 열수 추출방법이 효과적인 방법이라고 사료된다. LPS로 염증이 유도된 RAW 264.7 세포에서 복령추출물이 산수유 추출물에 비해 NO 생성 억제 효과가 우수한 것으로 확인되었다. MPP+에 의해 유도된 SH-SY5Y 신경세포에 복령 열수 추출물은 산수유 열수 추출물에 비해 산화적 스트레스로부터 신경세포 보호효과가 우수하게 나타났다. 복령과 산수유 추출방법에 따른 항산화, 항염 및 산화적 스트레스로부터의 신경세포 보호효과를 갖는 기능성 소재로서 가능성이 있다고 사료된다.

Keywords

References

  1. J. P. Kehrer, L. O. Klotz, "Free radicals and related reactive species as mediators of tissue injury and disease: implications for health", Crit. Rev. Toxico., Vol.45, pp. 765-798, (2015). https://doi.org/10.3109/10408444.2015.1074159
  2. W. J. Yoon, J. A. Lee, K. N. Kim, J. Y. Kim, S. Y. Park, "In vitro antiinflammatory activity of the Artemisia fukudo extracts in murine macrophage RAW 264.7 cells", Korean J. Food Sci. Technol, Vol. 39, pp. 464-469, (2007).
  3. V. N. Talesa, "Acetylcholine esterase in Alzheimer's disease", Mech Ageing Dev, Vol.122, pp. 1961-1969, (2001). https://doi.org/10.1016/S0047-6374(01)00309-8
  4. H. R. Jeong, J. H. Kwark, J. H. Kim, G. N. Choi, C. H. Jeong, H. J. Heo, "Antioxidant and neuronal cell protective effects of an extract of Houttuynia cordata Thumb(a culinary herb)", Korean J. Food Preserv., Vol. 17, pp. 720-726, (2010).
  5. M.S. Kwom, S. K. Chung, J. U. Choi, K. S. Song, W. W. Kang. "Quality and functional characteristics of cultivated Hoelen(Poria cocos Wolf) under the picking date. J. Korean Soc Food Sci Nutr., Vol. 27, pp. 1034-1040, (1998).
  6. N. Takao, N. Takahashi, M. Kobayash, S. Shoji. "A polysaccharide by laboratory cultivation of Poria cocos Wolf", Carbohydrate Research Vol. 87, pp. 161-164, (1980). https://doi.org/10.1016/S0008-6215(00)85202-7
  7. H. Kanayama, N. Adachi, M. Togami, "A new antitumor polysaccharide from the mycelia of Poria cocos Wolf", Chem Pharm Bull Vol. 31, pp. 1115-1119, (1993). https://doi.org/10.1248/cpb.31.1115
  8. H. Nukaya, H. Yamashiro, H. Fukazawa, H. Ishida, K. Tsuji, "Isolation of inhibitors of TPA-induced mouse ear edema from Hoelen, Poria cocos". Chem Pharm Bull Vol. 44, pp. 847-850, (1996). https://doi.org/10.1248/cpb.44.847
  9. S. R. Chung, K..H. Jeune, S. Y. Park, S. J. Jang, "Toxicity and lectins Constituents from the seed of Cornus officianalis". Korean J. Pharmacogn, Vol. 24, pp. 177-183, (1993).
  10. O. K. Kim, "Antidiabetic and actioxidative effects of Corni fructus in streptozotocininduced diabetic rats". J Korean Oil Chemists' Soc, Vol. 22, pp. 157-167, (2005).
  11. H. K. Joo, D. J. Jang, "Effects of shanshuyu(Cornus officinalis Sieb) tea and market of rat". Korean J Dietary Culture, Vol. 4, pp. 257-264, (1989).
  12. K. I. Seo, S. W. Lee, K. H. Yang, "Antimicrobial and antioxidative activities of Corni fructus extracts". Korean J Food Preserv, Vol. 6, pp. 99-103. (1999).
  13. Y. B. Seo, G. J. Kil, Y. K. Lee, Y. C. Lee, "Study on the effects of Corni fructus about the anti-allergic action". Korean J Herboogy Vol. 1 pp. 1-17, (2002).
  14. Y. J. Kim, D. Y. Son, "Antioxidant activity and suppression of pro-inflammatory mediator of Corni fructus extracts in activated RAW 264.7 macrophage", Korean J. Food preserv., Vol.26, pp. 876-882, (2016).
  15. O. Folin, W. Denis, "On phosphotungastic phosphomolyblic compounds as color regents", J Biol Chem, Vol.12, pp. 239-249 (1912). https://doi.org/10.1016/S0021-9258(18)88697-5
  16. M. S. Blois, "Antioxidant determinations by the ese of a stable free radical', Nature, No. 181, pp. 1199-1200 (1958). https://doi.org/10.1038/1811199a0
  17. R. Roberta, P. Nicoletta, P. Anna, Y. Min, R. E. Catherine, "Antioxidant activity applying an improved ABTS radical cation decolorization assay", Free Radical Bio Med., No. 26, pp. 1231-1237 (1999). https://doi.org/10.1016/S0891-5849(98)00315-3
  18. K. H. Sohn, J. S. Kim, "Anti-dementia effects of Cornus officinalis S. et Z. extract on the scopolamine Induced dementia in mouse", Kor. J. Pharmacogn. Vol.48, pp 304-313, (2017).