• Applied Microscopy
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• v.37 no.2
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• pp.83-91
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• 2007

The present study examined the epidermal changes of adhesive disks which occur during attachment in Parthenocissus tricuspidata using scanning and transmission electron microscopy. Several adhesive disks, each covered with a bract, develop from the shoot apical meristem during early development. In the initial stage, the adhesive disks are club-shaped and their upper and lower epidermis are indistinguishable. However, in the actively growing stage, they become spherical and both epidermis are clearly differentiated into the adventitious roots. Prior to wall attachment, the adhesive disks exhibit adaxial convex and abaxial concave shapes, and electron-dense substances are abundant in the vacuoles of epidermal cells. The peripheral area of the adhesive disk is adhered first to the wall surface, while the central area is drawn inward in a vacuum-like state during attachment. As the attachment progresses and the electron-dense substances continue to discharge, the upper and lower epidermis rapidly undergo deterioration and the disks shrink considerably. At this stage, structural changes of the lower epidermis occur much faster than in the upper one. The discharged substance is accumulated on the wall surface, and this aids the attachment of adhesive disks on the wall for long periods. In this manner, the shape and structure of the adhesive disk epidermis change drastically from initial growth to the mature stage. Further, the role of electron-dense substance and shrinkage of the disk during attachment has been discussed in Parthenocissus tricuspidata.

• Proceedings of the Korea Water Resources Association Conference
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• 2007.05a
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• pp.1145-1148
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• 2007

한국을 비롯하여 논농사 중심지역에서는 하천 주변이 과거에는 농경지로, 그리고 오늘날은 도시지역으로 개발되어 하천의 원형이 거의 유지되지 못하고 있다. 하천은 수역과 수변으로부터 거리에 따라 여러 개의 이질적 생태적 공간이 이어지는 복합생태계로서 하나의 경관으로 볼 수 있다. 하천이 하나의 온전한 생태적 계로서 기능을 발휘하기 위해서는 이들을 모두 포함하는 하천의 원형이 복원되어야 한다. 현존하는 하천복원사업이 수변 구역의 복원에 초점을 맞추고 있지만, 진정한 복원이 이루어지기 위해서는 온전한 강변 식생의 복원이 선결과제이다. 본 연구에서는 유적군락의 형태로 잔존하는 강변 식생을 조사하여 식생복원 모델로 삼고자 한다. 수위와 침수체제에 의해 하천의 횡단구조를 수변, 홍수터 및 제방으로 구분한 후 각 지소의 적합한 식생단위를 구분하여 생태적 복원을 실현하기 위한 정보를 구축하였다. 수변식생으로는 달뿌리풀군락과 갈대군락이 자주 출현하고, 홍수터에는 갯버들군락과 키버들군락이 우세하였다. 그 중 전자의 식물군락은 하상재료가 거친 입자의 지소에 성립하고, 후자의 것은 미세한 입자의 지소에 성립하는 경향이었다. 제방역에는 버드나무군락, 다릅나무군락, 귀룽나무군락, 물푸레나무군락, 산사나무군락, 신나무군락(한탄강 지류), 참느릅나무군락, 물푸레나무-갈참나무군락(한탄강 본류), 오리나무군락(민통선 북방지역), 소나무군락, 황철나무군락, 느릅나무군락(소양강 상류), 왕버들군락(금강 중류) 등이 성립하였다. 이러한 식생정보를 하천의 지리적인 위치에 따라 구분하였고, 생태적 복원 시 도입식물을 결정하기 위한 자료로 삼기 위해 각 식생단위를 이루는 종 조성을 정보 체계화하였다.을 효율적으로 하기 위하여 Solar Unit으로부터 나오는 전기를 중전기에서 밧데리로 축전을 시키고 완전 충전 후에는 나머지 전기는 방전이 되도록 회로를 구성하였다. 사통수문 자원조사 결과에 의하면 현재 저수지에 물공급을 하는 수문은 취수탑 형식이 70% 이상을 차지하고 있으며 나머지 30%의 사통수문 중 원형수문비가 98% 이상을 차지하고 있다. 현재 대체에너지를 사용하는 저수지 사통수문은 없는 것으로 조사되었으며 전력을 사용하는 사통도 조사결과에 의하면 20% 이내로 나타났다. 이러한 결과는 향후 수리시설 개보수시 적은 예산으로 사업을 시행하는 경우 사통수문의 설치방향의 지표가 될 것으로 판단되며 수리시설의 운영관리에 대한 새로운 대안으로 제시할 수 있다.분류되었다. 지표 유거가 많아 배수등급이 매우양호로 분류되던 토양은 정 등(1995)의 분류와 비교하여 대부분 강우 유출 가능성이 큰 쪽으로 조정되었다. 새로운 수문학적 토양유형을 이용할 경우 낮은 토심에서 암반층이 발견되는 산림토양이 분포한 유역이나 산림, 밭 등에 식질 토양이 많이 분포하는 유역에서는 기존의 방법을 이용하는 것보다 강우 유출량이 높게 평가될 것으로 판단된다. 앞으로 강우 유출량 실측자료와의 비교를 통해 지속적인 보정을 하여야 할 것이며 특히 불투수층의 존재시 일괄적으로 D유형으로 분류된 토양의 경우 깊이에 따라 C 또는 D 유형으로 세분하여 조정할 필요가 있다.가 있었다. 에틸아세테이트분회물의 경우 디글로로메탄 분회물에 비해 다소 낮은 저해효과를 나타내었지만 250 ${\mu}$g/ml 농도에서 약 60%의 세포독성 효과를 나타내었다. 디클로로메탄 분획물과 에틸아세테이트 분

• Ecology and Resilient Infrastructure
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• v.9 no.4
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• pp.237-246
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• 2022

Owing to their high bioproductivity and unique physical environment, estuarine wetlands are gaining importance in national biodiversity management and habitat conservation. With regard to conservation and management of estuarine wetlands, this study analyzed the ecological characteristics of Gahwacheon Estuarine Wetland, an open estuary with various habitat types. Data from vegetation and biotic surveys have shown that 12 plant communities of five physiognomic vegetation types, including lentic herbaceous vegetation, halophytic herbaceous vegetation, and chasmophytic herbaceous vegetation. Due to the discharge of Namgang Dam and the effect of the tide, vegetation are distributed along the narrow waterside area. In terms of biodiversity, a total of 715 species, including 12 endangered wildlife species, were identified. Species diversity was relatively high in sections I and III where various riverbed structures and microhabitats were distributed. Due to the effect of the brackish water area following the inflow of seawater, endangered wildlife of various functional groups were also found to be distributed, indicating the high conservation value of that area. The collection of ecological information of the Gahwacheon Estuarine Wetland can be used as a framework for establishing the basis for conservation and management of the estuarine ecosystem and support policy establishment.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.19 no.10
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• pp.2028-2042
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• 1994

Properly defining signal and noise in a self-organizing system like ART(Adaptive Resonance Theory) neural network model raises a number of subtle issues. Pattern context must enter the definition so that input features, treated as irrelevant noise when they are embedded in a given input pattern, may be treated as informative signals when they are embedded in a different input pattern. The ATR automatically self-scales their computational units to embody context and learning dependent definitions of a signal and noise and there is no problem in categorizing input pattern that have features similar in nature. However, when we have imput patterns that have features that are different in size and nature, the use of only one vigilance parameter is not enough to differentiate a signal from noise for a good categorization. For example, if the value fo vigilance parameter is large, then noise may be processed as an informative signal and unnecessary categories are generated: and if the value of vigilance parameter is small, an informative signal may be ignored and treated as noise. Hence it is no easy to achieve a good pattern categorization. To overcome such problems, a Coupled-ART neural network capable of modularized categorization of patterns is proposed. The Coupled-ART has two layer of tightly coupled modules. the upper and the lower. The lower layer processes the global features of a pattern and the structural features, separately in parallel. The upper layer combines the categorized outputs from the lower layer and categorizes the combined output, Hence, due to the modularized categorization of patterns, the Coupled-ART classifies patterns more efficiently than the ART1 model.

• Korean Journal of Environment and Ecology
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• v.25 no.2
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• pp.156-165
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• 2011

It is selected seven sites for marine algal flora and community structure and investigated seasonally from December 2008 to November 2009 in Gogunsan Islands, the west coast of Korea. A total of 58 species including 10 green, 16 brown and 32 red algae were collected and identified. Among these species, 11 species were found throughout the year. Annual mean biomass in dry weight were 213.8 g $m^{-2}$ at Sinsido 1, 143.1 g $m^{-2}$ at Sinsido 2, 133.3 g $m^{-2}$ at Sinsido 3, 164.0 g $m^{-2}$ at Munyeodo, 116.9 g $m^{-2}$ at Seonyudo, and 145.1 g $m^{-2}$ at Jangjado. Maximum biomass was recorded in Sinsido 1, and minimum mean biomass was Sinsido 4. The dominant species based on biomass were Sargassum thunbergii, Ulva pertusa, Sargassum fusiforme and Corallina pilulifera. S. thunbergii was the representative alga occurred at all seasons. The flora investigated could be classified into six functional groups such as coarsely branched form (46.6%), filamentous form (27.6%), sheet form (17.2%), thick leathery form (3.4%), jointed calcareous form (3.4%) and crustose form (1.7%). The R/P, C/P and (R+C)/P value reflecting flora characteristics were 0.33~0.75, 1.11~2.50, 1.47~3.25, respectively. Diversity index (H') and dominance index (DI) indicated that stability of seaweed community of Gogunsan Islands was unstable and environmental conditions were bad. According to multidimensional scaling (MDS) and cluster analysis, Gogunsan Islands were divided into three distinct groups. The first group was Sinsido 2, Sinsido 3, Seonyudo and Muyeodo and the second group comprised Sinsido 1 and Jangjado and the other was Sinsido 4 due to meaningful difference in similarity.

• Journal of Applied Biological Chemistry
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• v.57 no.4
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• pp.379-386
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• 2014

Chromatin is an instructive DNA structure that can widely respond to external signals. An important change of chromatin is the modifications of histone for this regulation. There are accumulating lists of these modifications and the complexity of their action is gradually understood. It is evident that histone modifications play important roles in most biological processes that are involved in the expression or repression of DNA. The surface of nucleosomes is susceptible to multiplicity of modifications. Chromatin modifications can play either by eliminating chromatin contacts or by recruiting non-histone proteins to chromatin. Many of these regulations seem to be epigenetically inherited. Thus, histone modifications are closely correlated with many fundamental biological processes in animal, plant and microbial kingdoms. Failures of histone modification lead, in general, to defective chromosome condensation or decondensation, impeding many biological functions including development, maturation, and protection against various diseases.

• Korean Journal of Microbiology
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• v.52 no.4
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• pp.487-494
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• 2016

In Saccharomyces cerevisiae, Paf1 complex consists of five proteins, and they are structurally and functionally well conserved in yeast, fruit fly, plants, and human. With binding to RNA polymerase II from transcription start site to termination site, Paf1 complex functions as a platform for recruiting many types of transcription factors to RNA polymerase II. Paf1 complex contributes to H2B ubiquitination and indirectly influences on H3K4 di- and tri-methylation by histone crosstalk. But the individual effects of five components in Paf1 complex on these two histone modifications including H2B ubiquitination and H3K4 methylation largely remained to be identified. In this study, we constructed the single-gene knockout mutants of each Paf1 complex component and observed H3K4 mono-, di-, and trimethylation as well as H2B ubiquitination in these mutants. Interestingly, in each ${\Delta}paf1$, ${\Delta}rtf1$, and ${\Delta}ctr9$ strain, we observed the dramatic defect in H3K4 monomethylation, which is independent of H2B ubiquitination, as well as H3K4 di- and trimethylation. However, the protein level of Set1, which is methyltransferase for H3K4, was not changed in these mutants. This suggests that Paf1 complex may directly influence on H3K4 methylation by directly regulating the activity of Set1 or the stability of Set1 complex in an H2B ubiquitination independent manner.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.30 no.2
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• pp.263-278
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• 2004

Ursolic acid (UA) and Oleanolic acid (ONA), known as urson, micromerol and malol, are pentacyclic triterpenoid compounds which naturally occur in a large number of vegetarian foods, medicinal herbs, and plants. They may occur in their free acid form or as aglycones for triterpenoid saponins, which are comprised of a triterpenoid aglycone, linked to one or more sugar moieties. Therefore UA and ONA are similar in pharmacological activity. Lately scientific research, which led to the identification of UA and ONA, revealed that several pharmacological effects, such as antitumor, hepato-protective, anti-inflammatory, anticarcinogenic, antimicrobial, and anti-hyperlipidemic could be attributed to UA and ONA. Here, we introduced the effect of UA and ONA on acutely barrier disrupted and normal hairless mouse skin. To evaluate the effects of UA and ONA on epidermal permeability barrier recovery, both flanks of 8-12 week-old hairless mice were topically treated with either 0.01-0.1mg/mL UA or 0.1-1mg/mL ONA after tape stripping, and TEWL (transepidermal water loss) was measured. The recovery rate increased in those UA or ONA treated groups (0.1mg/mL UA and 0.5mg/mL ONA) at 6h more than 20% compared to vehicle treated group (p < 0.05). Here, we introduced the effects of UA and ONA on acute barrier disruption and normal epidermal permeability barrier function. For verifying the effects of UA and ONA on normal epidermal barrier, hydration and TEWL were measured for 1 and 3 weeks after UA and ONA applications (2mg/mL per day). We also investigated the features of epidermis and dermis using electron microscopy (EM) and light microscopy (LM). Both samples increased hydration compared to vehicle group from 1 week without TEWL alteration (p < 0.005). EM examination using RuO4 and OsO4 fixation revealed that secretion and numbers of lamellar bodies and complete formation of lipid bilayers were most prominent (ONA=UA > vehicle). LM finding showed that thickness of stratum corneum (SC) was slightly increased and especially epidermal thickening and flattening was observed (UA > ONA > vehicle). We also observed that UA and ONA stimulate epidermal keratinocyte differentiation via PPAR Protein expression of involucrin, loricrin, and filaggrin increased at least 2 and 3 fold in HaCaT cells treated with either ONA (10${\mu}$M) or UA (10${\mu}$M) for 24 h respectively. This result suggested that the UA and ONA can improve epidermal permeability barrier function and induce the epidermal keratinocyte differentiation via PPAR Using Masson-trichrome and elastic fiber staining, we observed collagen thickening and elastic fiber elongation by UA and ONA treatments. In vitro results of collagen and elastin synthesis and elastase inhibitory activity measurements were also confirmed in vivo findings. These data suggested that the effects of UA and ONA related to not only epidermal permeability barrier functions but also dermal collagen and elastic fiber synthesis. Taken together, UA and ONA can be relevant candidates to improve epidermal and dermal functions and pertinent agents for cosmeseutical applications.

• Journal of The Korean Association For Science Education
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• v.22 no.4
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• pp.872-881
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• 2002

Student questioning is included in the priority of science literacy, to enable students to solve problems by exploring questions, communicating and constructing knowledge(AAAS, 1989). Also, the essence of student questioning in science lies in its function as a link between thinking and learning. But educators did not pay much attention to students' questioning in Korea. The purpose of this study was to investigate the effects of science question enhancement instruction on students' science questioning level and achievement. Also, this study showed the effects of other variables(logical thinking, science achievement, interest, and gender) on students' science questioning level. The pretest-posttest control group design group design was used. The sample was consisted of 80 second grade middle school students in experimental group(Science question enhancement instruction) and 74 students in control group(Traditional learning). Students in both groups were received identical content instruction on the unit 'Structures and functions of plant'. These groups were treated for 15 hours during 6 weeks. Students' questions were rated using the four levels described by the Middle School Students' Science Question Rating Scale(r= .96,)(Cuccio-Schirripa & Steinner, 2000). Science achievement data were collected using a 17 item multiple choice test(Cronbach ${\alpha}$= .84). To investigate students' logical thinking ability, a abridged GALT(Cronbach ${\alpha}$= .69) was used. Five-way ANOVA, ANCOVA, and multiple regression analysis were used to analyze the results. The results indicated that students who received instruction on researchable questioning outperformed those students who were not instructed on high-order questioning(p< .01). Results of correlations indicated that instruction(r= .640), science achievement(r= .311) and logical thinking ability(r= .212) was significantly and positively related with students' questioning level. But, interest and gender did not show any significant correlation with students' questioning level. Science question enhancement instruction was more effective on science achievement than the traditional instruction(p< .01).

• Journal of Korean Society of Forest Science
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• v.101 no.2
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• pp.175-184
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• 2012

This study was conducted to develop new mattress systems with vegetation base materials for protecting stream bank and rapid rehabilitation. Vegetation base materials are primarily compounded with fine soil, organic composts and peat moss as plant fibers, a water retainer and a soil improver. Peat moss can usually provide necessary natural fibers and organic materials in soil. Especially, peat moss can absorb up to 25 times its own weight in water and is therefore valued as a water retainer to prevent drying effect of vegetation base materials which can harm the growth of vegetation in mattresses. Normally mattress systems resist the lateral earth pressures or stream power by their own weight. Therefore, filled materials must have suitable weight, compressive strength and durability to resist the loading, as well as the effects of water and weathering. In this project, 100 to 200-mm clean, hard stones were basically specified, and about 50-mm rubbles were also used. Test application of new mattress system carried out in the stream bank of a small stream in the Gwangreung experimental forest, belonging to Korea Forest Research Institute (KFRI) in December 16th, 2006. As a result of the monitoring of vegetation coverage of test application plots (each plot size is 4 by 2 m), the coverage of all plots reached 100% in the end of May, 2007 (approximately 50 days passed after the first gemination of vegetation). The coverage of the plots using big hard stones and organic composts and the plots containing peat moss increased more rapidly. The results show that peat moss is effective to retain soil moisture and establish more sound environment for vegetation gemination.