• Horticultural Science & Technology
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• v.28 no.5
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• pp.828-835
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• 2010

Conventional methods for identification of apple cultivars are based on the evaluation of sets of morphological characteristics, however, closely related cultivars often cannot be distinguished by morphological traits. This study was conducted to develop DNA markers for discrimination of the apple cultivars bred in Korea. Thirty random primers generated eighty-three random amplified polymorphic DNA (RAPD) markers from thirty-one Korean bred and introduced apple cultivars. Fifty-two RAPD fragments were cloned and sequenced for conversion into sequence characterized amplified region (SCAR) markers. Among them only seventeen SCAR markers resulted in the amplification of single major bands the same size as the RAPD fragment cloned. Several combinations of six (AN11_433, AN08_566, A408_592, AK17_653, AO04_711, AO04_779 or AW15_368, AN11_433, A408_592, AK17_653, AO04_711, AO04_779, or AL1_427, AN11_433, AN08_566, A408_592, AK17_653, AO04_779) to seven (AL1_427, AN11_433, AN08_566, A408_592, AK17_653, AM16_708, AO04_779 or A330_424, AN11_433, AG14_502, AN08_566, A408_592, AK17_653, AO04_779 or A330_424, AN11_433, AK14_564, A408_592, AK17_653, AM16_708, AT14_789) SCAR markers provided enough polymorphism to identify sixteen Korean apple cultivars among thirty-one tested cultivars. Therefore, application of the seventeen SCAR markers was sufficient to identify the thirty-one tested apple cultivars. These markers could be utilized as a reliable tool for cultivar discrimination of Korean apples.

• Clean Technology
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• v.26 no.2
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• pp.145-150
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• 2020

Nitrous oxide (N₂O) is one of the six greenhouse gases, and it is essential to reduce N₂O by showing a global warming potential (GWP) equivalent to 310 times that of carbon dioxide (CO₂). Selective catalytic reduction (SCR) is a technology that converts ammonia into harmless N₂ and H₂O by using ammonia as a reducing agent to remove NOx, one of the air pollutants; the process also produces high denitrification efficiency. In this study, the Fe-BEA catalyst was steam-treated at 100 ℃ for 2 h before Fe ion exchange in the fixed bed reactor in order to investigate the effect of the steam-treated Fe-BEA catalyst on the NH₃-SCR reaction. NH₃-SCR reaction test of synthesized catalysts was performed at WHSV = 180 h^-1, 370 to 400 ℃ in the fixed bed reactor. The Fe-BEA(100) catalyst steam-treated at 100 ℃ showed a somewhat higher activity than the Fe-BEA catalyst at 370 to 390 ℃. The catalysts were characterized by BET, ICP, NH₃-TPD, H₂-TPR, and ²⁷Al MAS NMR in order to determine the cause affecting NH₃-SCR activity. The H₂-TPR result confirmed that the Fe-BEA(100) catalyst had a higher reduction of isolated Fe³⁺ than the Fe-BEA catalyst, and that the steam treatment increased the amount of isolated Fe³⁺ as an active species, thus increasing the activity.

• Journal of Korean Society of Forest Science
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• v.94 no.6
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• pp.488-495
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• 2005

This study was conducted to understand the influences of forest structure on throughfall, stemflow and interception loss. The study plots included the natural old-growth deciduous, Pinus koraiensis and Abies holophylla forests in Gwangneung and the rehabilitated young mixed forest in Yangju, Gyeonggido. The Pinus koraiensis and Abies hotophylla had been planted in 1976. The rehabilitated young mixed forest had been established to control erosion in 1974. Total and net rainfall were monitored from March, 2003 to October, 2004. Tipping bucket rain gauge recorded total rainfall. Throughfall and stemflow were measured by custom-made tipping bucket and CR10X data logger at each $10m{\times}10m$ plots at intervals of 30 minutes. Interception loss in the Pinus koraiensis plot were most as 37.2% of total rainfall and least as 22.6% in the rehabilitated young mixed forest. Stemflow in the rehabilitated young mixed forest was 10.7% of total rainfall and stemflow in the Pinus koraiensis plot was 2.4%. The average throughfall ratio ranged from 66% to 77% depending on the canopy coverage. The relationship of stemflow and total rainfall represented in a linear regression equation though the variation of data was large. The ratio of stemflow-conversion was 2% of total rainfall in the Pinus koraiensis plot and 12% in the rehabilitated young mixed forest, respectively. The stem storage of the natural old-growth deciduous was the largest of 0.21 mm whereas that of the Pinus koraiensis plot was the least of 0.003 mm. A deciduous forest produced stemflow more than a coniferous forest due to a smooth bark and steeply angled branches. Interception loss of all study plots increased linearly as total rainfall increased. The distribution of interception loss data related in total rainfall became wider in a deciduous forest than a coniferous. It resulted from seasonality of leaf area index in a deciduous forest. As considered above results, it was confirmed that there were great differences of throughfall, stemflow and interception loss depending on forest stand structures. The simulation model for predicting interception loss must have parameters such as forest stand characteristics and LAI in order to describe the influence of forest structure on interception loss.

• Development and Reproduction
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• v.8 no.2
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• pp.119-122
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• 2004

It is well known that unidentified factors in sera, hormones and growth factors promote the proliferation of granulosa cells and nuclear maturation of bovine COCs in vitro. Attemps had been developed the simple composition of culture media and similar system to in vivo conditions has been applied. In the present study, we investigated the effect of TGF-${\beta}$ on in vitro maturation and in vitro development of Hanwoo COCs. When the COCs were matured in TCM 199 containing 0.1, 1 or 10 ng/ml TGF-${\beta}$ for 24 hrs, metaphaseⅡ of COCs were obtained 95.8%, 100% of matured COCs, respectively and there were no differences among the concentrations of TGF-${\beta}$. Matured COCs with TGF-${\beta}$ cultured in maturation medium after in vitro fertilization, developmental rate to blastocyst were 0~0.8%. Matured COCs with TGF-${\beta}$ were cultured in TCM 199+10% FBS, 0.8% BSA, 0.1% PVA, blastocyst formation were showed in 12.4%, 12.8%, 8.5% of those and cultured in IVMD or IVD without serum were 38.4%, 34.8%, respectively. There were significant differences among the media (P<0.05). TGF-${\beta}$ is available for i vitro maturation of bovine COCs, but further investigation would be need for finding the synergistic autocrine/paracrine fashion of other growth factors in early bovine development.

• Journal of the Society of Disaster Information
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• v.17 no.3
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• pp.415-426
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• 2021

Purpose: The purpose of this study is to create the deveopment plan for promotion of advanced disaster-safety awareness, which is noted as a major factor in the large disaster. Method: This study is to conduct theoretical review with regard to disaster management and safety awareness. Consciousness surveys on safety awareness and previous disaster case was analyzed to derive the cause of the disaster, and the development plan for promotion of advanced disaster-safety awareness was suggested. Result: In the survey on the public's sense of safety on the disaster management evaluation, 'Response' stage was well performed, but the 'Recovery' stage was not. Especially, it was found that disaster safety education at the 'Prevention' stage was very lacking. In the survey on the public's safety awareness, the awareness level of the evacuation facility was very low, information on infectious diseases and collapse accident was insufficient. Especially, it has been found that the awareness on safety regulation in daily life is very insufficient. Through the case study on previous disaster(COVID-19, Fire in Miryang Sejong Hospital, Forest fire in the east coas at 2004'), it was derived that the lack of safety awareness(such as safety insensitivity) was the main factor of the expansion of the damage scale. Conclusion: The development plan for promotion of advanced disaster-safety awareness are as follow. First, it is necessary to spread the safety culture movement through the expansion of safety education and safety promotion. Second, disaster confrontation training for the public should be implemented to improve the effectiveness of disaster response. Finally, it is necessary to change the individual awareness on safety. When these factors are implemented systematically, advanced disaster-safety awareness can be promoted. Ultimately, disaster accidents in our society can be reduced.

• Journal of the Daesoon Academy of Sciences
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• v.44
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• pp.177-212
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• 2023

In this study, discussions of the suppression of cultural diversity in communities was conducted. First, based on the studies conducted so far and recent changes, the oppression that exists between the Chinese government and ethnic minorities was considered. The visible suppression mentioned was the expansion of Han Chinese Mandarin language education, sanctions on minority languages, and the expansion of higher education at the exclusion of minority identities. In terms of 'invisible' oppression, urbanization, urban development with modernization at the forefront, and the use of officials from minority ethnic groups educated by the central government were items that were discussed. Next, the case of Latin America was examined. In particular, attention was paid to the theory of resistance against Europeans and European culture. Based off of the worries and experiences of Latin American intellectuals who have underwent oppression as individuals from culturally diverse backgrounds, a mature theory was formulated that could be used to defend Chinese minorities in the future. There is a specificity to the problem of Chinese minority communities. However, from a large perspective, experience and self-critical exploration in Latin America serve as an opportunity to expand the specificity of Chinese minority communities. Their situation resembles previous situations in Latin America when native cultures were being culturally eroded by Europe. Thus, as Latin American scholars argue, a shift in perception is necessary. In addition to this, in the text, it is likewise necessary to reflect on diversity, freedom, and mutualistic respect. There are proposals advocating for the realization of Heyibutong (和而不同 harmony but not through sameness) based on the situation in China. In the process of this consideration, much thought was given about what the observed communities are like and what a hypothetically desirable community would be like. This extends not only to Chinese minority communities and native residents of Latin America, but also to Asians in the United States and foreigners in Korea. Through this, it is hoped that desirable communities characterized by cultural diversity can be skillfully pursued.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.12 no.3
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• pp.159-169
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• 2007

Excessive input of organic matters from fish cage farms to the coastal waters has been considered as one of the major factors disturbing their benthic ecosystem. Sediment samples were taken from around the two fish cage zones (A and B) in Tongyeong coast in June and August 2003, to evaluate the ecological impacts of fish cage farming activity on the macrobenthic polychaete communities. Polychaete accounted for $81{\sim}87%$ of the total macrofauna individuals from each of the sampling stations. The number of species, abundance, diversity and dominant species of polychaete were rapidly changed with the distance from the fish cages. Within 10 m from the fish cages, Capitella capitata, which is a bio-indicator for the highly enriched sediments, was a dominant species and the lowest diversity was recorded. In particular, the maximum density (${\sim}18,410\;ind.m^2$) of C. capitata was found at Farm A where fish cages were more densely established within a semi-enclosed bay system. The sampling zone between 10 m and 15 m showed a rapid decrease of C. capitata with a rapid increase of the numbers of species, implying that this zone may be an ecotone point from a highly to a slightly enriched area. In the sampling zone between 15 m and 60 m, a transitional zone, which represents slightly enriched condition before normal one, was observed with additional increase and maintenance of the number of species and density of polychaete. In addition, the potential bio-indicators of organic enrichment, such as Lumbrineris longifolia and Aphelochaeta monilaris were the predominant species in the sampling zone. Multidimensional scaling (MDS) ordination plots and k-dominance curves confirmed the above results on the gradual changes in the macrobenthic polychaete communities. Our findings suggest that the magnitude of impact of fish cage farming activity on polychaete communities is probably governed by a distance from fish cage, density of fish cage and geomorphological characteristics around fish cage farm.

• Food Science and Preservation
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• v.30 no.1
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• pp.146-154
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• 2023

To develop a multi-functional ingredient, the bioconversion of katsuobushi protein was optimized using Bacillus subtilis HA and Lactobacillus plantarum KS2020. The Dendropanax morbiferus extract (DME) culture with protease activity (102 unit/mL) was prepared by B. subtilis with 2% glucose and 1% skim milk through one day of alkaline fermentation. Katsuobushi protein was effectively hydrolyzed by the DME culture at 60℃ for 3 hours, resulting in a tyrosine content of 156.85 mg%. Subsequently, a second lactic acid fermentation was carried out with 10% monosodium glutamate (MSG) using L. plantarum KS2020 to produce higher levels of GABA. Following co-cultivation for three days, DME exhibited a pH of 8.3 (0% acidity). After seven days, the viable cell count of L. plantarum increased to 9.33 CFU/mL, but viable Bacillus cells were not detected. Taken together, a multi-functional ingredient with enriched GABA, peptides, probiotics, and umami flavor was developed through lactic acid fermentation using hydrolyzed katsuobushi protein. These results indicate that katsuobushi protein could be used as a byproduct to produce a palatable protein hydrolysate using alkaline-fermented DME culture as a proteolytic enzyme source.

• Journal of dental hygiene science
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• v.18 no.2
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• pp.85-96
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• 2018

The purpose of this study is to propose learning objectives in social dental hygiene by analyzing and reviewing learning objectives in oral health administration area of the existing public oral health. This study is a cross-sectional study. The subjects of the study selected with convenience extraction were 15 members of the social dental hygiene subcommittee of the Korean Society of Dental Hygiene Science. Data collection was conducted by self-filling questionnaire. The research tool is from 48 items of A division in the book of learning objectives in the dental hygienist national examination, and this study classified each of them into 'dental hygiene job relevance', 'dental hygiene competency relevance', 'timeliness', and 'value discrimination of educational goal setting' to comprise 192 items. Also, to collect expert opinions, this study conducted Delphi survey on 7 academic experts. Statistical analysis was performed using the IBM SPSS Statistics ver. 23.0 program (IBM Co., Armonk, NY, USA). Recoding was performed according to the degree of relevance of each learning objective and frequency analysis was performed. This study removed 18 items from the whole learning objectives in the dental hygienist national examination in the oral health administration area of public oral health. Fifteen revisions were made and 15 existing learning objectives were maintained. Forty-five learning objectives were proposed as new social dental hygiene learning objectives. The topics of learning objectives are divided into social security and medical assistance, oral health care system, oral health administration, and oral health policy. As a result of this study, it was necessary to construct the learning objectives of social dental hygiene in response to changing situation at the time. The contents of education should be revised in order of revision of learning objectives, development of competency, development of learning materials, and national examination.

• Journal of the Korean Geotechnical Society
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• v.20 no.5
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• pp.145-159
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• 2004

The conventional method for assessment of liquefaction potential proposed by Seed and Idriss has been widely used in most countries because of simplicity of tests. Even though various data such as stress, strain, stress path, and excess pore water pressure can be obtained from the dynamic test, especially, two simple experimental data such as the maximum deviatoric stress and the number of cycles at liquefaction have been used in the conventional assessment. In this study, a new detailed assessment for liquefaction potential to reflect both characteristics of real earthquake motion and dynamic soil resistance is proposed and verified. In the assessment, the safety factor of the liquefaction potential at a given depth of a site can be obtained by the ratio of a resistible cumulative plastic shear strain determined through the performance of the conventional cyclic test and a driving cumulative plastic shear strain calculated from the shear strain time history through the ground response analysis. The last point to cumulate the driving plastic shear strain to initiate soil liquefaction is important for this assessment. From the result of cyclic triaxial test using real earthquake motions, it was concluded that liquefaction under the impact-type earthquake loads would initiate as soon as a peak loading signal was reached. The driving cumulative plastic shear strain, therefore, can be determined by adding all plastic shear strains obtained from the ground response analysis up to the peak point. Through the verification of the proposed assessment, it can be concluded that the proposed assessment for liquefaction potential can be a progressive method to reflect both characteristics of the unique soil resistance and earthquake parameters such as peak earthquake signal, significant duration time, earthquake loading type, and magnitude.