• Title/Summary/Keyword: 수집종

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Purification of a New Elicitin from Phytopthora cambivora KACC40160 (Phytophthora cambivora KACC 40160으로부터 새로운 elicitin의 분리)

  • Yoon, Sang-Hong;Bae, Shin-Chul;Park, In-Cheol;Koo, Bon-Sung;Kim, Young-Hwan;Yeo, Yun-Soo
    • Applied Biological Chemistry
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    • v.46 no.2
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    • pp.79-83
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    • 2003
  • Elicitins, proteinaceous elicitors secreted from Oomycetes fungi (Phytophthora spp. and Pythium spp.), have been known as inducer of hypersensitive response (HR) in incompatible interactions between plant and pathogens. Five elicitins among many Korean Phytophthora species caused the reactions of distal HR in radish, chinese cabbage and some hot pepper cultivars, but not in cucumber and tomato. Because the isolation of elicitin from Phytophthora cambivora hasn't been reported yet, we have purified a cambivorein, a new member of the elicitin family, from the culture filtrate of Phytophtilora cambivora (KACC 40160) by using FPLC (Fast Protein Liquid Chromatography, AKTA) with sepharose S and Sephacryl HR columns. We confirmed that it induces necrosis activities in some hot pepper cultivars and its molecular weight is about 10 KDa by Tricine-SDS-PAGE. Comparison of amino acid sequences of its N-terminal ends also informed the identification of Iysine at the 13th position, which is characteristic of a kind of basic elicitin isoform $({\beta}-isoform)$. It Also showed that our elicitin is not identical with N-terminal sequences of many elicitins reported from Phytophthora spp..

Device Mutual Authentication and Key Management Techniques in a Smart Home Environment (스마트 홈 환경에서 디바이스 상호 인증 및 키 관리 기법)

  • Min, So-Yeon;Lee, Jae-Seung
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.10
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    • pp.661-667
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    • 2018
  • Recently, the smart home market is growing due to the development of wireless communication technology and sensor devices, and various devices are being utilized. Such an IoT environment collects various vast amount of device information for intelligent services, receives services based on user information, controls various devices, and provides communication between different types of devices. However, with this growth, various security threats are occurring in the smart home environment. In fact, Proofpoint and HP warned about the cases of damage in a smart home environment and the severity of security vulnerabilities, and cases of infringement in various environments were announced. Therefore, in this paper, we have studied secure mutual authentication method between smart nodes used in smart home to solve security problems that may occur in smart home environment. In the case of the proposed thesis, security evaluations are performed using random numbers and frequently updated session keys and secret keys for well-known vulnerabilities that can occur in IoT environments and sensor devices such as sniffing, spoofing, device mutual authentication, And safety. In addition, it is confirmed that it is superior in security and key management through comparison with existing smart home security protocol.

Estimation of genetic parameters for growth traits and backfat thickness using Maternal animal model in pigs (모체효과 모형을 이용한 돼지 품종 간의 성장형질 및 등지방두께에 대한 유전모수 추정)

  • Kim, Yong-Min;Choi, Tae-Jeong;Cho, Eun-Seok;Cho, Kyu-Ho;Chung, Hak-Jae;Jeong, Yong-Dae
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.11
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    • pp.350-356
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    • 2017
  • This study was conducted to examine the influence of the maternal genetic effect of swine on their economic traits through the estimation of their genetic parameters, breeding value and genetic trends using an animal model. The data on Duroc pigs, Korean Native Pigs and Synthetic pigs (Duroc ${\times}$ Korean Native Pig) from 2000 to 2015 were obtained from the National Institute of Animal Science in Korea and used to estimate the genetic parameters for the average daily gain (ADG) and backfat thickness (BFT). Model 1 included the additive genetic effect of the animals, Model 2 consisted of Model 1 + the maternal genetic effect and Model 3 consisted of Model 2 + the maternal permanent environment effect. The heritability calculated by estimating the additive genetic effect was higher than that calculated by estimating the maternal genetic effect using the maternal animal model. The estimated genetic correlations between the additive and maternal genetic effects for the ADG and BF were strongly negative. Thus, the estimation of the breeding value can be used to select the most appropriate individuals and make an optimal breeding scheme.

Effects of Participation in Korean Traditional Dance on the Acculturative Stress and Life Satisfaction in the Married Immigrant Women (한국전통무용프로그램이 결혼이주여성의 문화적응 스트레스 및 생활만족에 미치는 영향)

  • Lee, Moon-Sook;Le, Hyung-Il;Lee, Jung-Joo;Yoon, Min-Sook;Kim, Sang-Hyun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.12 no.6
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    • pp.2567-2574
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    • 2011
  • The purpose of this study was to investigate the effects of Korean traditional dance on acculturative stress and life satisfaction in the married immigrant women. The subjects of this quasi-experimental study were two folds as one experimental group and one control group. The subjects of experimental group were randomly composed of Korean traditional Dance. Contrary to this, the subjects of control group included those who neither participated in Korean traditional dance. All statistical analyses and description methods were computed by SPSS window 14.0. The discriminant analysis was used to find changes of the acculturative stress and life satisfaction, ANCOVA. The results of this study were as followings: Frist, there was founded that Korean traditional dance program group significantly decreased their acculturative stress. Second, there was founded that Korean traditional dance program group significantly improved their life satisfaction. It is concluded that Korean traditional dance program is directly and strongly cause and effect relations with the acculturative stress and life satisfaction in the married immigrant women.

Related Factors with Decreased Visual Acuity of Elementary School Students (초등학생의 시력저하 관련요인)

  • Mun, Kyeoung-Ae;Lee, Moo-Sik;Na, Baeg-Ju;Lee, Jin-Yong;Hong, Jee-Young;Bae, Seok-Hwan;Yoo, Se-Jong
    • Proceedings of the KAIS Fall Conference
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    • 2010.11b
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    • pp.677-680
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    • 2010
  • 본 연구는 경기도 구리시의 2개 초등학교 5, 6학년 학생을 대상으로 시력 저하 실태 및 그와 관련된 요인들을 알아보고 시력 저하 예방교육의 기초자료로 활용하고자 시도 된 연구이다. 자료의 수집은 2009년 11월 1일부터 12월 10일까지 초등학교 5학년, 6학년 학생 793명(남 417명, 여 376명)이었고 2009학년도의 시력검사자료 분석과 문헌 및 기존의 조사도구를 토대로 개발한 자가 보고식 설문지를 이용하여 조사하였다. 자료 분석은 SPSS WIN 12.0(한글판)프로그램을 이용하여 빈도, 백분율, $x^2$-test 및 로지스틱 회귀분석의 통계적 방법으로 분석하였다. 시력저하에 유의한 영향요인으로 좌측시력을 종속변수로 한 경우 중 일반특성에 따른 변수인 부모 및 형제와 자매가 안경 및 렌즈 착용하는 경우, 경제적으로 어려울수록 시력 저하 자가 많았고, 환경특성에 따른 변수인 거실 및 방안밝기가 어두울수록, 컴퓨터 사용 시 실내밝기가 어두울수록, TV의 눈높이 및 컴퓨터 모니터의 눈높이가 높을수록 시력 저하 자가 많았다. 우측시력을 종속변수로 한 경우 중 일반특성에 따른 변수인 부모가 안경 및 렌즈를 착용할수록, 형제 와 자매가 안경 및 렌즈를 착용할수록, 경제적으로 어려울수록 시력 저하 자가 많았고, 환경특성에 따른 변수인 거실 및 방안밝기가 어두울수록, 컴퓨터 사용 시 실내밝기가 어두울수록, TV의 눈높이 및 컴퓨터 모니터의 눈높이가 높을수록 시력 저하 자가 많았다. 안경 및 렌즈를 종속변수로 한 경우 중 일반특성에 따른 변수인 부모 및 형제와 자매가 안경 및 렌즈를 착용할수록, 경제적으로 어려울수록 시력 저하 자가 많았으며, 환경특성에 따른 변수인 거실 및 방안밝기의 실내밝기가 어두울수록, TV의 눈높이 및 컴퓨터 모니터의 눈높이가 높을수록 시력 저하 자가 많았다. 눈 건강상태를 종속변수로 한 경우 중 일반특성에 따른 변수인 경제적으로 어려울수록 시력 저하 자가 많았고, 건강행동특성에 따른 변수인 주관적인 건강상태가 나쁠수록, 눈 피로감이 있을수록 시력 저하 자가 많았으며 환경특성에 따른 변수인 거실 및 방안밝기의 실내 밝기가 어두울수록 시력 저하 자가 많았다. 시력저하관련 요인을 연구한 결과 학생들의 시력 저하 자가 매년 증가하고 있다는 것을 알 수 있었고, 유전적 요인에 의한 시력저하는 개인의 노력에 의하여 사전예방이 어렵지만 환경적 요인에 의한 시력저하는 노력과 관심으로 사전예방이 가능하므로 학생들의 시력건강증진을 위하여 학부모와 학교 및 전문 의료기관이 서로 연계적인 협조 하에 학생들 개개인에 맞는 효율적인 시력증진관리프로그램 개발하는 것이 필요하고, 현행학교시력검사의 정확성을 기하기 위하여 표준화된 시력검사 지침을 개발하여 사용할 필요가 있으며, 지역 및 환경적 배경이 다르다 보니 연구결과가 다르게 나타나는 경우가 있는데 객관적인 결과를 도출하기 위하여 여러 지역의 학생을 대상으로 다양하고 체계적인 연구를 해야 할 필요성이 있다고 생각한다.

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Comparative Study on Effects of Sexuality Education in Elementary School Students (초등학생의 성교육 효과에 관한 비교연구)

  • Seo, Soon-Hee;Hong, Jee-Young;Lee, Moo-Sik;Na, Baeg-Ju;Lee, Jin-Yong;Bae, Seok-Hwan;Yoo, Se-Jong
    • Proceedings of the KAIS Fall Conference
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    • 2010.11b
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    • pp.513-516
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    • 2010
  • 본 연구는 성교육이 학생들의 성 지식, 태도, 역할 정체감에 미치는 효과를 알아보기 위해 실험군과 대조군으로 집단을 나누어 실험군에게 의도적인 성교육 실시하여 그 결과를 살펴보고 향후 학교에서 실시되는 성교육 계획 수립에 도움을 주고자 하는 실험연구이다. 본 연구의 대상은 충청북도 제천시에 위치한 J초등학교 5학년 6개 학급을 대상으로 실험군 100(남 50, 여 50), 대조군 100(남 50, 여 50) 총 200명을 대상으로 실시하였으며 자료 수집은 2009년 10월 15일부터 2009년 11월 25일까지 실시하였다. 연구도구는 문헌고찰을 통해 성 지식, 성 태도, 성역할 정체감에 사용한 설문지를 구성타당도 검증을 통해 문항을 구성 선정하였으며, 신뢰도는 Cronbach's ${\alpha}$ 값으로 검증하였다. 연구 대상자의 일반적 특성을 보면 92%의 학생이 부모님이 있고, 88.5%가 형제자매가 있으며, 집안 분위기는 60%가 자유스럽고, 7%가 엄한 것으로 나타났다. 연구 대상자의 성관련 특성으로 가정에서 성교육을 받아본 경험이 있는 경우는 36%로 나타났으며, 성과 관련된 정보를 얻는 순서는 87.5%의 학생이 학교 보건선생님한테 성 관련 정보를 얻는 경우가 대부분이었고, 다음 순서로는 부모님, 서적류, 담임선생님, 영상매체, 친구, 형제자매 순으로 나타났다. 연구 대상자의 신체변화정도를 살펴보면 여자의 경우 월경이 있는 경우는 20%정도, 가슴의 변화는 84%로 나타났으며, 남자의 경우 몽정이 있는 경우는 4%로 나타났으며, 남녀 모두 변성기, 여드름, 발모, 몽정, 월경, 가슴변화 등 한 가지 이상 신체변화가 나타난 경우는 56%로 나타났다. 연구 대상자의 성지식 사전 사후 점수를 비교해보면 두 집단 모두 사전보다 사후 점수가 증가하였다. 사후검사에서 교육을 받지 않은 대조군의 성지식 점수는 소폭 증가하였으나 성교육을 실시한 실험군의 성지식 점수는 더 큰 폭으로 높았으며, 특히 신체발달에서 사전검사에 대조군은(M=2.17), 실험군은 (M=1.66)으로 실험군의 점수가 낮았으나, 사후검사에서는 대조군은(M=2.76), 실험군은(M=3.90)으로 실험군의 점수가 더 높았다. 이상의 결과를 종합해보면 학교에서의 체계화된 성교육은 학생들의 성 지식, 태도, 성 역할 정체감 형성에 도움을 줄뿐만 아니라 성교육을 부끄러워하고, 쑥스러워하며, 어색해 하던 아이들이 문제 중심 학습을 적용하여 자기 주도적으로 문제를 해결하고, 체험, 실습, 역할극, 토의 등의 활동적인 수업에 적극 참여하고, 자연스럽게 활동하여 긍정적인 '밝은 성(性)'으로 변해가는 모습을 관찰할 수 있었다. 성교육은 인간의 성장과 발달에 따라 변화하는 과정이라는 것을 인식하여 건전한 성, 건강한 성을 함양하기 위해 체계적이고 발달 단계에 맞추어 이루어져야 할 것이다.

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Physicochemical Properties, Lactic Acid Bacteria Content and Physiological Functionalities of Korean Commercial Makgeolli (시판 막걸리의 이화학적 특성과 젖산균 함량 및 생리기능성)

  • Kim, Young-Hun;Min, Jin-Hong;Kang, Min-Gu;Kim, Jae-Ho;Ahn, Byung-Hak;Kim, Ha-Kun;Lee, Jong-Soo
    • Microbiology and Biotechnology Letters
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    • v.40 no.4
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    • pp.325-332
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    • 2012
  • The goal of this study was to characterize the physicochemical properties, lactic acid bacteria and physiological functionality of Korean commercial Makgeolli. We collected 60 kinds of raw and pasteurized Makgeolli commercially available in Korea. Their physicochemical properties, lactic acid bacteria content and physiological functionalities were determined. In the commercial raw Makgeolli, ethanol and residual sugar content ranged from 3.6% to 9.6% and 0.20% to 4.52%, respectively. Furthermore, the raw Makgeolli contained 3.50 to 101.9 mg% of amino nitrogen. In the pasteurized Makgeolli, ethanol content ranged from 4.0% to 7.0% and their residual sugar content were 0.98% to 7.57%. The raw Makgeolli contained 2.0 $ent^3$~1.2 $ine^8$ CFU/mL of lactic acid bacteria. Among several functionalities, the antihypertensive angiotensin I-converting enzyme inhibitory activities of EDS-14 raw Makgeolli and PWR-12 pasteurized Makgeolli were very high, at 89.9% and 87.0% respectively. The other functionalities were seen to be below 30% or not detectable. The ${\beta}$-Glucan contents of the raw and pasteurized Makgeolli were noted as average, at 14.1% and 14.6%, respectively.

A Study on the Genomic Patterns of SARS coronavirus using Bioinformtaics Techniques (바이오인포매틱스 기법을 활용한 SARS 코로나바이러스의 유전정보 연구)

  • Ahn, Insung;Jeong, Byeong-Jin;Son, Hyeon S.
    • Proceedings of the Korea Contents Association Conference
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    • 2007.11a
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    • pp.522-526
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    • 2007
  • Since newly emerged disease, the Severe Acute Respiratory Syndrome (SARS), spread from Asia to North America and Europe rapidly in 2003, many researchers have tried to determine where the virus came from. In the phylogenetic point of view, SARS virus has been known to be one of the genus Coronavirus, but, the overall conservation of SARS virus sequence was not highly similar to that of known coronaviruses. The natural reservoirs of SARS-CoV are not clearly determined, yet. In the present study, the genomic sequences of SARS-CoV were analyzed by bioinformatics techniques such as multiple sequence alignment and phylogenetic analysis methods as well multivariate statistical analysis. All the calculating processes, including calculations of the relative synonymous codon usage (RSCU) and other genomic parameters using 30,305 coding sequences from the two genera, Coronavirus, and Lentivirus, and one family, Orthomyxoviridae, were performed on SMP cluster in KISTI, Supercomputing Center. As a result, SARS_CoV showed very similar RSCU patterns with feline coronavirus on the both axes of the correspondence analysis, and this result showed more agreeable results with serological results for SARS_CoV than that of phylogenetic result itself. In addition, SARS_CoV, human immunodeficiency virus, and influenza A virus commonly showed the very low RSCU differences among each synonymous codon group, and this low RSCU bias might provide some advantages for them to be transmitted from other species into human beings more successfully. Large-scale genomic analysis using bioinformatics techniques may be useful in genetic epidemiology field effectively.

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Knowledge, Attitude, and Performance on the Hand Washing of Health Care related Students (보건계 대학생의 손씻기에 대한 지식, 태도 및 수행도)

  • Ryu, Seong-Mi
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.14 no.8
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    • pp.3916-3924
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    • 2013
  • The purpose of this study is to provide the students with the proper hand washing knowledge after examining their attitude and performance of health science college students. Data were collected from 425 students at the college in G metropolitan city by using a questionnaire. Data were analyzed into descriptive statistics, ANOVA and pearson correlation coefficient with SPSS/WIN18.0. The average frequency and the average time of hand washing were 8.7 times a day and 18.5 sec respectively. The average frequency of hand washing education was 2.4 times. Soap bar was most frequently used as a hands detergent and paper towel as a dry method.. The Frequency and the time(sec) of hand washing increased significantly in the following provisions: above 6 times hand washing education, practical education, decontaminating agent soap, and use of paper towel. Knowledge, attitude and performance on hand washing increased significantly in the following variable : women, practice at upper grade general hospital, above 11 times hand washing a day, above 16secs hand washing, above 6 times hand washing education, practical education, decontaminating agent soap, and use of paper towel. Finally, there was significantly difference among the knowledge, attitude, and performance of hand washing. The result of this study indicates a necessity for developing education programs consistently to improve knowledge, attitude, and performance of hand washing.

COBie-based Building Information Exchange System Framework for Building Facility Management (건축물 유지관리를 위한 COBie기반 건축정보교환체계 프레임웍 연구)

  • Kang, Tae-Wook
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.21 no.8
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    • pp.370-378
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    • 2020
  • The Construction Operations Building Information Exchange (COBie) collects and documents a large amount of information from different paths in one place during design and construction projects. This documented information is readily available as a means of continuously transferring data to the facility management systems used by building engineers. In this process, the COBie Worksheet, an open standard form, is used to input the information by simplifying the list required by the user. As a result, COBie was developed to improve dramatically how relevant information is obtained and updated to support operations, maintenance, and asset management at the design and construction stages. On the other hand, to use COBie, a great deal of manual work is required for information linkage and quality inspection with heterogeneous data models. These issues become obstacles to COBie-based facility management system development. This study analyzed the COBie information system and defined the framework for simpler operating maintenance information from BIM (Building Information Modeling). Moreover, the rules for facility management information submission, quality inspection, role definition of framework components, and information linkage were derived. COBie DB schema and support data linkages could be generated effectively based on the proposed framework in prototype development.