• 한국균학회소식:학술대회논문집
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• 2018.05a
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• pp.44-44
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• 2018

Fusarium head blight (FHB) caused by infection with Fusarium graminearum leads to enormous losses to crop growers, and may contaminate grains with a number of Fusarium mycotoxins that pose serious risks to human and animal health. Antagonistic bacteria that are used to prevent FHB offer attractive alternatives or supplements to synthetic fungicides for controlling FHB without the negative effects of chemical management. Out of 500 bacterial strains isolated from soil, Bacillus amyloliquefaciens JCK-12 showed strong antifungal activity and was considered a potential source for control strategies to reduce FHB. B. amyloliquefaciens JCK-12 produces several cyclic lipopeptides (CLPs) including iturin A, fengycin, and surfactin. Iturin A inhibits spore germination of F. graminearum. Fengycin or surfactin alone did not display any inhibitory activity against spore germination at concentrations less than 30 ug/ml, but a mixture of iturin A, fengycin, and surfactin showed a remarkable synergistic inhibitory effect on F. graminearum spore germination. The fermentation broth and formulation of B. amyloliquefaciens JCK-12 strain reduced the disease incidence of FHB in wheat. Furthermore, co-application of B. amyloliquefaciens JCK-12 and chemical fungicides resulted in synergistic in vitro antifungal effects and significant disease control efficacy against FHB under greenhouse and field conditions, suggesting that B. amyloliquefaciens JCK-12 has a strong chemosensitizing effect. The synergistic antifungal effect of B. amyloliquefaciens JCK-12 and chemical fungicides in combination may result from the cell wall damage and altered cell membrane permeability in the phytopathogenic fungi caused by the CLP mixtures and subsequent increased sensitivity of F. graminearum to fungicides. In addition, B. amyloliquefaciens JCK-12 showed the potential to reduce trichothecenes mycotoxin production. The results of this study indicate that B. amyloliquefaciens JCK-12 could be used as an available biocontrol agent or as a chemosensitizer to chemical fungicides for controlling FHB disease and as a strategy for preventing the contamination of harvested crops with mycotoxins.

• 한국균학회소식:학술대회논문집
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• 2018.05a
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• pp.13-13
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• 2018

Alternaria is a ubiquitous fungal genus, widely distributed in the environment and a range of different habitats. It includes both plant pathogenic and saprophytic species, which can affect crops in the field or cause post-harvest spoilage of plant fruits and kernels. Numerous Alternaria species cause damage to agricultural products including cereal grains, fruits and vegetables, and are responsible for severe economic losses worldwide. Most Alternaria species have the ability to produce a variety of secondary metabolites, which may play important roles in plant pathology as well as food quality and safety. Alternariol (AOH), alternariol monomethyl ether (AME), tenuazonic acid (TeA), tentoxin (TEN) and altenuene (ALT) are considered the main Alternaria compounds thought to pose a risk to human health. However, food-borne Alternaria species are able to produce many additional metabolites, whose toxicity has been tested incompletely or not tested at all. Both alternariols are mutagenic and their presence in cereal grain has been associated with high levels of human esophageal cancer in China. TeA exerts cytotoxic and phytotoxic properties, and is acutely toxic in different animal species, causing hemorrhages in several organs. The possible involvement of TA in the etiology of onyalai, a human hematological disorder occurring in Africa, has been suggested. Altertoxins (ALXs) have been found to be more potent mutagens and acutely toxic to mice than AOH and AME. Other metabolites, such as TEN, are reported to be phytotoxins, and their toxicity on animals has not been demonstrated up to now. Vegetable foods infected by Alternaria rot are obviously not suitable for consumption. Thus, whole fresh fruits are not believed to contribute significantly with Alternaria toxins to human exposure. However, processed vegetable products may introduce considerable amounts of these toxins to the human diet if decayed or moldy fruit is not removed before processing. The taxonomy of the genus is not well defined yet, which makes it difficult to establish an accurate relationship between the contaminant species and their associated mycotoxins. Great efforts have been made to organize taxa into subgeneric taxonomic levels, especially for the small-spored, food associated species, which are closely related and constitute the most relevant food pathogens from this genus. Several crops of agricultural value are susceptible to infection by different Alternaria species and can contribute to the entry of Alternaria mycotoxins in the food chain. The distribution of Alternaria species was studied in different commodities grown in Argentina. These food populations were characterized through a polyphasic approach, with special interest in their secondary metabolite profiles, to understand their full chemical potential. Alternaria species associated with tomato, bell pepper, blueberry, apples and wheat cultivated in Argentina showed a surprisingly high metabolomic and mycotoxigenic potential. The natural occurrence of Alternaria toxins in these foods was also investigated. The results here presented will provide background for discussion on regulations for Alternaria toxins in foods.

• 한국균학회소식:학술대회논문집
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• 2018.05a
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• pp.33-33
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• 2018

Air-borne plant pathogenic fungus Fusarium graminearum and seed-borne plant pathogenic bacterium Burkholderia glumae are cause similar disease symptoms in rice heads. Here we showed that two pathogens frequently co-isolated in rice heads and F. graminearum is resistant to toxoflavin produced by B. glumae while other fungal genera are sensitive to the toxin. We have tried to clarify the resistant mechanism of F. graminearum against toxoflavin and the ecological reason of co-existence of the two pathogens in rice. We found that F. graminearum carries resistance to toxoflavin as accumulating lipid in fungal cells. Co-cultivation of two pathogens resulted in increased conidia and enhanced chemical attraction and attachment of the bacterial cells to the fungal conidia. Bacteria physically attached to fungal conidia, which protected bacterium cells from UV light and allowed disease dispersal. Chemotaxis analysis showed that bacterial cells moved toward the fungal exudation compared to a control. Even enhanced the production of phytotoxic trichothecene by the fungal under presence of toxoflavin and disease severity on rice heads was significantly increased by co-inoculation rather than single inoculation. This study suggested that the undisclosed potentiality of air-born infection of bacteria using the fungal spores for survival and dispersal.

• Korean Journal of Weed Science
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• v.30 no.2
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• pp.164-170
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• 2010

In organic agriculture, cover crops are used for weed control. Cover crops inhibited weed germination or growth by allelopathy or shading effects. In this study, we used rye as cover crop to control weed in organic soybean field. The main treatment was rye using method such as living mulch, incorporating and mowing. Sub-treatment was rye planting density and soybean planting density. One month after soybean sowing, weed emergency and growth had been highly suppressed by rye in all treatments. Living mulch treatment was the best effective way to control the weed about 92%, and mowing treatment was the second about 75% compare to control. The weed control efficiency between rye planting densities was similar during one month. Soybean planting density treatment was same result as rye planting density. As a result of this experiment, we recommend rye as living mulch in one row planting.

• Journal of the Korean Society for Library and Information Science
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• v.54 no.1
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• pp.277-301
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• 2020

The purpose of this study is to analyze art museum websites and derive implications for future operation of art museum websites. To this end, this research collected basic information about art museums according to '2018 National General Report of Cultural Infrastructure' and investigate the websites of 30 art museums through multi-step sampling process. This study analyzed the structure and menu of the art museum websites as well as the current state of various contents provided by the websites and the search service for the collections offered by the websites. Following sentences are the results. First of all, the art museum websites offer 5.6 top menus on average. Secondly, contents related to art museum, exhibitions, news, education, general forum, and SNS are the basic contents that should be provided on the art museum websites. Third, for contents related to news, education, and events have problems with hierarchical structure and need to be adjusted. Fourth, in the content type, specialized information contents are relatively insufficient and thus need to be improved. Fifth, the art museum websites should give sufficient information about the collection and offer directory searching hat includes keyword searching as well as detail searching service. It is also required to reorganize the directory along with the download function for searched results and the sorting service.

• The Korean Journal of Applied Statistics
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• v.30 no.4
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• pp.603-613
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• 2017

Many people have recently posted about personal interests on social media. The development of the Internet and computer technology has enabled the storage of digital forms of documents that has resulted in an explosion of the amount of textual data generated; subsequently there is an increased demand for technology to create valuable information from a large number of documents. A text mining technique is often used since text-based data is mostly composed of unstructured forms that are not suitable for the application of statistical analysis or data mining techniques. This study analyzed the Meteorological Yearbook data of the Korea Meteorological Administration (KMA) with a text mining technique. First, a term dictionary was constructed through preprocessing and a term-document matrix was generated. This term dictionary was then used to calculate the annual frequency of term, and observe the change in relative frequency for frequently appearing words. We also used regression analysis to identify terms with increasing and decreasing trends. We analyzed the trends in the Meteorological Yearbook of the KMA and analyzed trends of weather related news, weather status, and status of work trends that the KMA focused on. This study is to provide useful information that can help analyze and improve the meteorological services and reflect meteorological policy.

• Journal of The Korean Association For Science Education
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• v.34 no.6
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• pp.601-611
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• 2014

The purpose of this study is to analyze the Korean science magazine, Science Donga providing scientific facts, phenomenons, and issues with infographic for the readers by time series analysis and to search for the application of infographic on the science education. The criteria for the infographic analysis of Science Donga consisted of three categories such as storytelling type, visual perception, and framework level because infographic presents complex information quickly and clearly by integrating various images, words, and graphics. We found that the articles emphasized by including image about science issue have been published from 1986 to 2014. Particularly, after 2008, the articles including infographic sharply rose. So we set up 2008 as $T_c$(Critical time point). The articles including infographic after 2008 have been more variously distributed and frequently used in storytelling types category such as location, time, number, connection, function, and process based infographic, in visual perception of Gestalt Theory such as proximity, similarity, continuation, and closure than before 2008. Lastly, in framework level category, location, time, number, and process based infographic mainly had total range level but function and connection based infographic changed in the framework level. The three features about storytelling type, visual perception, framework level are important changes to influence $T_c$ in the infographic analysis about Science Donga. Through the results of this study, we analyzed the feature of change on infographic from 1986 to 2014. Thus, we hope that the results suggest a basic criteria for making materials including infographic in science education.

• The Journal of Society for e-Business Studies
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• v.18 no.1
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• pp.33-55
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• 2013

Information sharing behavior in the Internet has raised much interest. Recently, social network sites provide a new information sharing channel for the users who want to connect with others based on common social background or tastes. Especially, we focus that a social network site becomes one of major routes for information sharing about socially influential issues. Therefore, studying how information is diffused via a social network site may give theoretically, practically significant implication. Based on the assertion, we investigated user's behavior to mediate other user's information messages. We define information mediating behavior as concurrent actions of filtering and distributing behavior of the digital content that is originated from one of the connected users. In this study, we intended to understand the effects of information mediating behavior, and tried to understand characteristics of re-mediating of previously mediated information. Using an agent-based simulation model, we found that information mediating behavior increased the extent of information diffusion significantly. In addition, even a small degree of mediating probability could boost up the level of information diffusion in the case of a re-mediating condition. We believe that those findings provide remarkable insight of research and business application on both of information sharing and diffusion in a social network site.

• The Korean Journal of Malacology
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• v.28 no.1
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• pp.13-19
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• 2012

To determine the status of Korean molluscs infection and relation with climate change of Korean peninsula, references were reviewed. Wild animals serve as intermediate, reservoirs and paratenic hosts to zoonotic parasites of human beings. Trematode are common parasites of molluscs and almost all trematodes infect mollusks as the first host in the life cycle, and most have a complex life cycle involving other hosts. The significance of auxiliary hosts to the ecology of the parasite has not been proper discussion. There is increasing concern as to the impact of climate change on the epidermiology of many parasitic diseases. A total of 21 species for snail transmitted parasites from Korean molluscs has been reviewed. Among them, 15 species was aquatic mollusks and 6 species for marine mollusks. Maximum infections belonged to Semisulcospira livertina had 11 kind of parasite pathogenic organisms, including Paragonimus westermani, Metagonimus yokogawai, Centrocestus armatus, Notocotylus magniovatus, Centrocestus formosanus, incerte cercaria, nipponensis cercaria, Yoshidae cercaria, cristata cercaria, innominatum cercaria and Metagonimus sp. And 11 in Parafossarulus manchouricus including Clonorchis sinensis, Asymphylodora japonica, Cyathocotyle orientalis, Exorchis oviformis, Notocotylus attenuatus, Echinochasmus japonicus, Loxogenes liberum, Cercariae of Loxogenes liberum Type I, Cercariae of Loxogenes liberum Type II, Furcocercus cercariae (Family Sanguincolidae) and Cercaria of Mucobucaris, 10 in Semisulcospira sp. including Paragonimus westermani, Metagonimus yokogawai, Centrocestus armatus, Echinochasmus redioduplicatus, Notocotylus magniovatus, Cercaria incerte, Cercaria nipponensis, Cercaria yoshidae, paludinarum cercaria and Metagonimus sp., 7 in Koreanomelania globus including Pseudexorchis major, Cercaria of parapleurolophocercous type, Metagonimus sp. (A & B), Cercaria nipponensis, Cercaria inserta and Cercaria yoshidae. Also, Tapes philippinarum have 3 pathogenic organisms including Cercariae tapidis, Cercariae furcocercus and Parvatrema sp. In particular, under climatic extremes such as floods and drought, aquatic molluscs may play a more prominent role in parasite transmission in the future.

• 한국균학회소식:학술대회논문집
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• 2006.10a
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• pp.32-44
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• 2006

To investigate non-aflatoxin-production of A. oryzae at the molecular level, an aflatoxin biosynthesis gene homolog cluster of RIB 40 was analyzed. Although most genes in the corresponding cluster exhibited from 97 to 99 % similarity to those of Aspergillus flavus, three genes shared 93 % similarity or less. In addition, although slight expression of aflR, positive transcriptional regulator gene, was detected in some A. oryzae strains having seven aflatoxin biosynthesis homologous genes, other genes related to aflatoxin production were not detected. RIB strains were mainly divided into group 1, having seven aflatoxin biosynthesis homologous genes (aflT, nor-i, aflR, norA, avnA, verB, and vbs), and group 2, having three homologous (avnA, verB, and vbs). Partial aflatoxin homologous gene cluster of RIB62 from group 2 was sequenced and compared with that of RIB40 from group 1. RIB62 showed a large deletion upstream of ver-1 with more than half of the aflatoxin homologous gene cluster missing including aflR, a positive transcriptional regulatory gene. Adjacent to the deletion of the aflatoxin homologous gene cluster, RIB62 has a unique sequence of about 8kb and a telomere. Southern analysis of A. oryzae RIB strains with four kinds of probe derived from the unique sequence of RIB62 showed that all group 2 strains have identical hybridizing signals. Polymerase chain reaction with specific primer set designed to amplify the junction between ver-1 and the unique sequence of RIB62 resulted in the same size of DNA fragment only from group 2 strains. Based on these results, we developed a useful genetic tool that distinguishes A. oryzae group 2 strains from the other groups' strains and propose that it might have differentiated from the ancestral strains due to chromosomal breakage.