• Journal of Life Science
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• v.20 no.9
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• pp.1314-1323
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• 2010

In this study, we constructed various kinds of binary vectors with the pPZP backbone for co-overexpression, tissue- or development-specific expression and stress-inducible expression, and validated them for ectopic expression of target genes. Using a modified CaMV 35S promoter, a binary vector was generated for co-overexpression of two different genes and was confirmed to be efficient for overexpressing two different target genes at the same time and place. Binary vectors containing At2S3, KNAT1 or LFY promoters were constructed for tissue-specific or development-specific gene expression, and the binary vectors were suited for embryo/young seedling stage-, shoot apical meristem- or leaf primordia-specific expressions. Furthermore, the binary vectors containing RD29A or AtNCED3 promoters were validated as suitable vectors for gene expression induced by abiotic stresses such as high salt, ABA, MV and low temperature. Taken together, the binary vectors constructed in this study would be very useful for analyzing the biological functions of target genes and molecular mechanisms through ectopic expression.

• Journal of the Earthquake Engineering Society of Korea
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• v.5 no.5
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• pp.73-83
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• 2001

The mass and stiffness of upper wall-lower frame system(mixed building structures) change sharply at transfer floor due to different structural system in upper and lower part. These mixed building structures generally show the stiffness, weight or geometric vertical irregularities. The purpose of this study is to investigate the response characteristics of these structures by push-over analysis and nonlinear time history analysis. For four types of analysed models, only the variation of upper wall stories was considered. The conclusions of this study are following; (1) In the push-over analysis, yielding hinges in beams and columns of lower frame occurred at the base shear of similar magnitude in all models. But as the number of stories of upper wall increases, yielding hinges at ends of coupling beams were observed in the small magnitude of base shear. (2) In the nonlinear time history analysis, yielding of lower frame occurred at beams with as small ground acceleration as 55gal, and in upper walls yielding was concentrated on coupling beams and shear walls near the transfer floor. (3) As the number of stories of upper walls decreases, the story stiffness of the lower frames decreased relatively and the occurrence of soft stories in the lower frame was observed.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.36 no.3
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• pp.185-192
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• 2023

In this study, fluctuating wind velocity for time history analysis is simulated by a single variate, single-dimensional random process using the KBC2022 spectrum about across-wind direction. This study analyzed and obtained the inelastic dynamic response for structures modeled as a single-degree-of-freedom system. It is assumed that the wind response is excellent in the primary mode, the change in vibration owing to plasticization is minor, along-wind vibration and across-wind vibration are independent, and the effect of torsional vibration is small. The numerical results, obtained by the Newmark-𝛽 method, shows the time-history responses and trends of maximum displacements. As a result of analyzing the inelastic dynamic response of the structure with the second stiffness ratio(𝛼) and yield displacement ratio (𝛽) as variables, it is identified that as the yield displacement ratio (𝛽) increases when the second stiffness ratio is constant, the maximum displacement ratio decreases, then reaches a minimum value, and then increases. When the stiffness ratio is greater than 0.5, there is a yield point ratio at which the maximum displacement ratio is less than 1, indicating that the maximum deformation is reduced compared to the elastically designed building even if the inelastic behavior is permitted in the inelastic wind design.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.4 no.2
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• pp.117-131
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• 2006

A coupled T-H-M(Thermo-Hydro-Mechanical) analysis was carried out for KENTEX (KAERI Engineering-scale T-H-M Experiment for Engineered Barrier System), which is a facility for validating the coupled T-H-M behavior in the engineered barrier system of the Korean reference HLW(high-level waste) disposal system. The changes of temperature, water saturation, and stress were estimated based on the coupled T-H-M analysis, and the influence of the types of mechanical constitutive material laws was investigated by using elastic model, poroelastic model, and poroelastic-plastic model. The analysis was done using ABAQUS, which is a commercial finite element code for general purposes. From the analysis, it was observed that the temperature in the bentonite increased sharply for a couple of days after heating the heater and then slowly increased to a constant value. The temperatures at all locations were nearly at a steady state after about 37.5 days. In the steady state, the temperature was maintained at $90^{\circ}C$ at the interface between the heater and the bentonite and at about $70^{\circ}C$ at the interface between the bentonite and the confining cylinder. The variation of the water saturation with time in bentonite was almost same independent of the material laws used in the coupled T-H-M processes. By comparing the saturation change of T-H-M and that of H-M(Hydro-Mechanical) processes using elastic and poroelastic material mod31 respectively, it was found that the degree of saturation near the heater from T-H-M calculation was higher than that from the coupled H-M calculation mainly because of the thermal flux, which seemed to speed up the saturation. The stresses in three cases with different material laws were increased with time. By comparing the stress change in H-M calculation using poroelasetic and poroelasetic-plastic model, it was possible to conclude that the influence of saturation on the stress change is higher than the influence of temperature. It is, therefore, recommended to use a material law, which can model the elastic-plastic behavior of buffer, since the coupled T-H-M processes in buffer is affected by the variation of void ratio, thermal expansion, as well as swelling pressure.

• Journal of the Korea Concrete Institute
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• v.21 no.4
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• pp.489-499
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• 2009

It is quite difficult to predict the flexural strength of post-tensioned members with unbonded tendons (unbonded posttensioned members, UPT members) because of debonding behavior between concrete and prestressing tendons, which is different from that with bonded tendons. Despite many previous researches, our understanding on the flexural strength of UPT members is still insufficient, and thus, national codes use different methods to calculate the strength, which quite often give very different results. Therefore, this paper reviews various existing methods, and aims at proposing an improved rational strength model for UPT flexural members having better accuracy. Additionally, a database containing a large number of test data on UPT flexural members has been established and used for verification of the proposed flexural strength model. The analysis results show that the proposed method provides much better accuracy than many existing methods including the rigid-body model that utilizes the assumption of concentrated deformation and plastic hinge length, and that it also gives proper consideration on the effects of primary parameters such as reinforcement ratio, loading pattern, concrete strength, etc. Especially, the proposed method also well predicts the ultimate stress of unbonded tendons of over-reinforced members, which are often possible in construction fields, and high strength concrete members.

• Journal of Korean Society of Steel Construction
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• v.22 no.5
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• pp.497-509
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• 2010

Five concrete-encased steel columns using high-strength steel($f_{ys}$=801MPa) and high-strength concrete($f_{ck}$=97.7MPa) were tested to investigate the eccentric axial load-displacement relationship. Test parameters included the type, yield strength, and spacing of lateral reinforcement, and also the eccentricity of axial load. To analyze the behavior of the column specimens, the nonlinear sectional analysis using strain-compatibility and confinement effect was performed. To examine the applicability of existing design codes for the composite sections using high-strength materials, the test results were also compared with the predictions by the nonlinear analysis and the design codes. The confinement effect of lateral reinforcement increased the ductility of concrete, and the moment capacity of the column specimens increased with the ductility of concrete. The prediction by the nonlinear analysis gave good agreement with the test results. On the other hand, the ACI 318 neglecting lateral confinement effect underestimated the strength of the column specimens, and the Eurocode 4 using complete plastic capacity of steel section overestimated.

• Journal of the Microelectronics and Packaging Society
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• v.10 no.1
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• pp.45-50
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• 2003

Global full 3D finite element analysis fatigue models are constructed for flip-chip BGA on system board to predict the creep fatigue life of solder joints during the thermal cycling test. The fatigue model applied is based on Darveaux's empirical equation approach with non-linear viscoplastic analysis of solder joints. The creep life was estimated the creep life as the variations of the four kinds of thermal cycling test conditions, pad structure, composition and size of solder ball. The shortest fatigue life was obtained at the thermal cycling test condition from $-65^{\circ}C$ to $150^{\circ}C$. It was increased about 3.5 times in comparison with that from $0^{\circ}C$ to $100^{\circ}C$. At the same conditions, the fatigue life of SMD structure as the change of pad structure increased about 5.7% as compared with NSMD structure. Consequently, it was confirmed that the fatigue life became short as the creep strain energy density increased in solder joint.

• Journal of Korean Society of Steel Construction
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• v.30 no.1
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• pp.37-48
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• 2018

Cyclic loading tests for shear dominant hybrid steel link beams with circular web openings were performed to evaluate the seismic performance. Four half-scaled specimens with bolted connections were tested. The test parameter is a diameter of the web opening, i.e., shear strength ratio ($V_{pw}/V_p$) of the link beam and presence of top-seat angles. Using test results, adequate design shear strength of link beam was finally suggested. Test results showed that when the shear capacity is less than half of the plastic shear strength, seismic performance was improved due to mitigation of pinching under reversed cyclic inelastic deformations.

• Journal of the Korean Geotechnical Society
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• v.29 no.10
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• pp.57-66
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• 2013

Societal interest on a faster transportation demands an increase of the train speed exceeding current operation speed of 350 km/h. To trace the pattern of variations in displacements and subsoil stresses in the concrete slab track system, finite element simulations were conducted. For a simple track-vehicle modeling, a mass-point system representing the moving train load was developed. Dynamic responses with various train speeds from 100 to 700 km/h were investigated. As train speeds increase the displacement at rail and subsoil increases nonlinearly, whereas significant dynamic amplification at the critical velocity has not been found. At low train speed, the velocity of elastic wave carrying elastic energy is faster than the train speed. At high train speed exceeding 400 km/h, however, the train speed is approximately identical to the elastic wave velocity. Nonlinearity in the stress history in subsoil is amplified with increasing train speeds, which may cause significant plastic strains in path-dependent subsoil materials.

• International Journal of Highway Engineering
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• v.12 no.4
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• pp.203-209
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• 2010

The base asphalt mixtures that used the waste recycle asphalt correcting from the four different overlay construction sites in Seoul city were made using the foamed asphalt method. The sample mixtures were made in different ratio of the recycle asphalt and new asphalt material and the performance of the mixtures of the different ratio was investigated in the laboratory. The laboratory tests includes the Marshall test, the indirect tensile test, the resilient modulus test, the creep test and the wheel tracking test. The test of the recycle foamed asphalt mixtures(RFA) were compared with the those of the recycle hot mix asphalt(RHA) mixtures. The performance of the RFA is comparable to that of the RHA. On the other hand, the indirect tensile strength of the RFA in dry condition is lower than that of RHA and the indirect tensile strength of the RFA in wet condition is much lower than that of the RHA.