• The Korean Journal of Cytopathology
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• v.4 no.1
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• pp.74-76
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• 1993

Cytologic findings of pleural effusion in three cases of rhabdomyosarcoma are reported. Case 1 was a pleomorphic rhabdomyosarcoma which had devoped at the chest wall of an elderly male patient and caused pleural effusion. The cytologic features were consistent with pleomorphic rhabdomyosarcoma, that was, showing loose clusters, cellular pleomorphism, and abundant finely vesicular cytoplasm. Cases 2 and 3 were embryonal rhabdomyosarcomas in young adults. Primary site was the oral cavity in case 1, but unknown in case 2 and case 3. The effusion cytology was similar in these cases. Clustered or isolated small round cells with hyperchromatic nuclei and scanty cytoplasm were smeared. The cohesiveness of tumor cells was weak and the cells did not show linear arrangement or nuclear melding. Effusion cytology in a sarcoma patient would be diagnostic when the primary site and the type of sarcoma were already known.

• Applied Microscopy
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• v.41 no.3
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• pp.163-168
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• 2011

Tiger barb (Puntius tetrazona Bleeker, 1855) is a teleost belonging to Cyprinidae. The oogenesis of tiger barb was investigated by light microscope. The ovary was of white and ellipsoidal shape with the major axis 2 cm and the minor axis 1 cm. Cytoplasm of oogonia was basophilic and many nucleoli were located at inside of nuclear membrane. In early stage of primary oocyte, yolk vesicles were distributed only in the marginal area. In secondary oocyte, the egg envelope was formed and yolk vesicles was formed in the cytoplasm. The basophilic substance of cytoplasm was changed to acidic. In case of matured egg, thickness of egg envelope and size of egg were increased. The yolk vesicles were changed to yolk mass in accordance with development. Also, there is not the formation of oil droplets in cytoplasm. In conclusion, the oogenesis of tiger barb was characterized by the increase in cell size, the formation of yolk, the decrease of basophilic substance in the cytoplasm, and formation of yolk mass.

• The Korean Journal of Malacology
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• v.27 no.1
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• pp.15-27
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• 2011

This study was performed to observe ultrastructure of the gill and to ascertain the effect of salinity on histopathological and ultrastructural changes in the gill of the Japanese clam, Corbicula japonica. Experimental period was 7 days. Experimental groups consisted of control, 5, 10, 20 psu. $LC_{50}$ (96 h.) by the probit was 19.55 psu. Mortality was significantly different from the control (p < 0.05). Inner demibranch of the gill of C. japonica was wider 1.37 times than outer demibranch (p < 0.001). The filament zone on the plica can be distinguished by the six epithelial celll cell; frontal ciliated epithelium ($7{\mu}m$), latero-frontal ciliated epithelium ($5{\mu}m$), postlatero-frontal epithelim ($3{\times}8{\mu}m$), and lateral ciliated epithelium ($5{\mu}m$) in the frontal zone, endothelial cellin the intermediate zone, and abfrontal cell in the abfrontal zone. It had one type of secretory cell that was filled with fibrous substances of low electron density. The gill of C. japonica exposed to 5 psu for 7 days was observed partially disappearance of the cilia, and glycogen granule in the filament. In the 10 psu, gill appeared partially modification of epithelial cell and destruction of the glycocalyx. Gill exposed to 20 psu was extended nuclus of the ciliated epithelial cell, destruction of the organelles, and observed glycogen granules infiltration and numerous vacuoles. Moreover, more than 50% filaments were observed that come out chitinous rod from disappearance of epithelial cell in the filament. Therefore, the destruction of the cilia and epithelial cell induce physiological activity and it may be leading directly to death.

• Journal of Life Science
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• v.28 no.10
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• pp.1127-1131
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• 2018

Cortisol, a steroid hormone, functions within metabolism, immune response, and stress. Intense or prolonged physical exercise increases cortisol levels to enhance the gluconeogenesis pathway and stabilize blood glucose level. However, cortisol also exerts a negative impact on muscle function and creates a stressful environment in skeletal muscle cells. The present study investigated the function of cortisol as a stress hormone. To examine the effect of the exercise-induced hormone cortisol on skeletal muscles, C2C12 cells were cultured and treated with cortisol at different concentrations. As a result, we found that the morphology of C2C12 changed remarkably with 5 ug/ml cortisol treatment. Western blot analysis was conducted to learn whether ER-stress and autophagy were induced. We found that the expression ratio of LC3I/LC3II decreased and BiP expression increased after cortisol treatment. In addition, immunocytochemistry analysis with IER3 antibody clearly showed that apoptosis is induced after 12-hour cortisol treatment. These results indicate that cortisol treatment could induce apoptosis, ER-stress, and autophagy in muscle cells. This study would provide valuable information in the study of the effects of exercise on skeletal muscle cells and the development of additives to reduce cortisol stress.

• Korean Journal of Animal Reproduction
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• v.19 no.4
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• pp.271-281
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• 1996

1960년대에 본격적인 연구가 시도된 난세포질내 정자직접주입법(Intracytoplasmic Sperm Injection ; ICSI)은 1976년에는 Uehara 등에 의한 hamster의 연구에서 최초로 전핵의 형성에까지 성공하였다. 이후 계속된 연구를 통하여 여러 동물종에서 이 방법에 의한 난자의 수정 및 배발달에 성공하여, 1988년에 토끼, 1991년에 소, 1995년에는 생쥐에서 산자의 생산에 성공하였다. 한편, 사람에 있어서는 1988년 Lanzendorf 등에 의해 최초로 사람난자가 난세포질내 정자직접주입법에 의해 수정에 성공한 것이 보고되었으며, 1992년에는 Palermo 등에 의해 이 방법에 의해 수정된 수정란 이식을 통한 임신 및 성공적인 분만이 보고되었다. 난세포질내 정자직접주입법에 있어서는 정자의 운동성 및 첨체반응 등의 유무가 수정에 관여하는 중요한 요인이 아닌 것으로 알려지고 있으며, 성숙한 정자가 아닌 정소상체(미부-두부)정자 혹은 정소내의 미성숙정자를 사용하여 난세포질내 정자직접주입법을 시행하여도 수정 및 임신이 가능한 것으로 보고되었다. 또한 정자세포(spematid)나 원형정자세포(round spermatid)를 수정과 배발달이 관찰되었으며 사람에 있어서는 분만까지 성공하였다. 현재까지 이 난세포질내 정자직접주입법은 학술적으로는 난자-정자가 결합하는 기전을 밝히는 연구에 이용되어 왔으며, 임상적으로는 시험관아기시술에 있어서 정자의 기능, 수 등이 문제가 되어 수정이 어려운 남성불임환자에게 적용하여 좋은 성과를 거두어 왔다. 향후 이 기술은 유전자진단이나 남성불임환자의 처치에 폭넓게 사용될 것으로 기대된다.

• Restorative Dentistry and Endodontics
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• v.6 no.1
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• pp.87-92
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• 1980

Two different types of cilia appeared in the odontoblasts with secretory function of the dentin forming substances. The cilia possessing the double nine peripheral fibrils and probably no central pairs of microtubuies with two basal centrioles in odontoblasts and odontoblastic process is speculated to be an indicative of sensory function. The other cilia with a single centriole may be ilssociated with the motile function agitating the extracellular dentin forming materials secreted from the odontoblast.

• Korean Journal of Microbiology
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• v.32 no.2
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• pp.139-146
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• 1994

Protective effects on subcellular localization of Photobacterium leiognathi CuZnSOD(PSOD) were examined in Escherichia coli SOD mutant cells on the treatment of paraquat, heat shock $(37^{\circ}C{\to}42^{\circ}C{\to})$, hydrogen peroxide and copper sulfatem respectively. The physiological characteristics of the periplasmic and cytoplasmic PSOD localized differently are dependent on the conditions in this experiment. Cells expressing SOD periplasmically in the treatments of paraquat and $H_2O_2$ respectively were somewhat better protective effects cells expressiong SOD cytoplasmically at comparable level and SOD expression level showed, the most consistently important variable. However, this was reversed in the treatments of heat shock and $CuSO_4$, respectively.

• Parasites, Hosts and Diseases
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• v.29 no.4
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• pp.363-370
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• 1991

인체감염이 다발하는 폐흡충증의 혈청학적 진단에서 항원으로 사용하는 성충추출액은 여러단계의 질환 이행과정을 진단하는 항원으로서 문제가 있다. 이를 해결하려면 먼저 추출액내의 성분단백질의 성상을 파악할 필요가 있다. 이 연구에서는 폐흡충 성충 추출액으로 면역시킨 BALB/c mice의 비장세포와 SP2/0 형질세포종 세포를 세포융합하여 제작한 단세포군항체를 이용하여 친화성크로마토그래피로 폐흡충 성충의 구성단백질의 일부를 순수분리하고 성상을 관찰하였다. 그 결과는 다음과 같다. 1. 세포융합으로 PFCK-21, PFCK-44, PFCK-136, PFCK-189 등 4종류의 단세포군 항체를 얻었다. 그중 PFCK-21과 PFCK44는 17k Da, PFCK-136은 23, 46, 92 kDa 단백질에 반응하였고 PFCK-189는 여러종류의 단백질에 반응하였다. 2. PFCK-44 단세포군항체를 고리로 친화성 크로마토그래피를 실시하여 분리한 성분 단백질은 disc-PAGE상 4번째에 위치하고 분자량이 17 kDa로 알려진 단밸질이었다. 이 단밸질은 17 kDa의 monomer로 판단하였다. 면역조직화학염색을 실시한 결과 이 단밸질은 장관 상피세포에 반응하고 있었다. 3. PFCK-136 단세포군항체로 순수분리한 단밸질은 disc-PAGE상 1번 단밸질(440 kDa)이었으며 환원성 SDS-PAGE에서는 23 kDa 단밸질이었다. 면역조직화학염색으로 이 단세포군항체는 충란내 세포에 강하게 반응하고 있었다.

• The Korean Journal of Zoology
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• v.31 no.1
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• pp.29-34
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• 1988

In order to investigate the role of nuclei and cytoplasm in early embryogenesis, interspecific nuclear transplantation was carried out Nuclei of one species of amphibian was transplanted into enucleated egg of another species. In interspecific hybrids between Rana species, development was arrested before or right after the dorsal lip formation. In intergenetic hybrids between Rana, Xenopus and axolod, developmental arrest took place at late blastula stage. Comparing the developmental capacity of each nudeocytoplasmic hybrid, the more distantly related the species, the earlier does development arrest. The general rule is that nuclear transfers between any amphibian species will form a regulary cleaved late blastula. Two plausible factors relate to limitation of tite developmental capacity of nudeocytaplasmic hybrids. One is an irreversible change in grafted nuclei and another is tunctional incompatibility between the recipient cytoplasm and transplanted nucleus. The later is postulated a more possible cause of the arrestrnent.

• The Korean Journal of Zoology
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• v.38 no.3
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• pp.330-339
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• 1995

In Helice tridens tridens, hylaine cells, small granulocytes, and large granulocytes were identified. Features of hyaline cells include a large nucleus in proportion to the cytoiplasm, and weak electron-dense granules of oval shape and vesicles, and endoplasmic reticulum (ER) in the cytoplasm. Small granulocytes have smaller nucleus than that of the hyaline cells, well-developed ER, Golgi complex, and small, round and electron-dense granules in the cytoplasm. Large granulocytes contain large and electron dense granules (ahout 1 $\mu$m) that fused small granules. Hemocytes of Helice tridens tridens differentiated from hyaline cell to large granulocyte granules of hyaline cells have lysosome and make small vesicles from nuclear envelopes. While these vesicles pass through the Golgi complex, they are filled with electron dense matetials, and then fused with the small granules. They eventually matured into large granules. All of hemocytes have the glycogen particles. In the large granulocytes heterogeneouse granules were supposed to occur by disappearance of granules.