• Journal of Life Science
• /
• v.26 no.7
• /
• pp.868-874
• /
• 2016

Receptor-interacting protein kinase 1 (RIPK1) and RIPK3 are members of the serine or threonine protein kinase superfamily that phosphorylates the hydroxyl group of serine or threonine through the highly conserved kinase region. The RIPK family plays a crucial role not only in inflammation and innate immunity, but also in mediating programmed cell death, such as apoptosis and necroptosis. The interaction between RIPK1 and other TNFR1-related proteins has been shown to assemble a signaling complex I that controls activation of the pro-survival transcription factor NF-κB upon binding of cytokines to TNF receptor 1 (TNFR1). Moreover, RIPK1 and RIPK3 interact through their RIP homotypic interaction motifs (RHIMs) to mediate programmed necrosis, which has long been considered an accidental and uncontrolled cell death form with morphological characteristics differing from those of apoptosis. Highly conserved sequences of RHIM in RIPK1 and RIPK3 were shown to regulate their binary interaction, leading to assembly of a cytosolic amyloid complex termed the “necrosome”. The necrosome also contains mixed lineage kinase domain-like protein (MLKL), which has been found recently to be a substrate of RIPK3 to mediate downstream signaling. This review provides an overview of the functional and physiological characteristics of RIPKs and MLKL in TNF signaling.

• Journal of Nutrition and Health
• /
• v.55 no.3
• /
• pp.330-347
• /
• 2022

Folate, a water-soluble vitamin, acts as a coenzyme for one-carbon metabolism in nucleic acid synthesis and amino acid metabolism. Adequate folate nutritional status during the periconceptional period is known to prevent neural tube defects. In addition, insufficient folate intake is associated with various conditions, such as anemia, hyperhomocysteinemia, cardiovascular disease, cancer, cognitive impairment, and depression. This review discusses the rationale for the revision of the 2020 Korean dietary reference intakes for folate, and suggestions for future revisions. Based on the changes in the standard body weight in 2020, the adequate intake (AI) for infants (5-11 months) and the estimated average requirements (EARs) for 15-18 years of age were revised, but there were no changes in the recommended nutrient intakes (RNIs) and tolerable upper intake levels (ULs) for all age groups. Mean folate intake did not reach RNI in most age groups and was particularly low in women aged 15-29 years, according to the results of the 2016-2018 Korea National Health and Nutrition Examination Survey (KNHANES). The percentages of folate intake to RNI were lower than 60% in pregnant and lactating women, but serum folate concentrations were higher than those in other age groups, presumably due to the use of supplements. Therefore, total folate intake, from both food and supplements, should be evaluated. In addition, the database of folate in raw, cooked, and fortified foods should be further expanded to accurately assess the folate intake of Koreans. Determination of the concentrations of erythrocyte folate and plasma homocysteine as well as serum folate is recommended, and quality control of the analysis is critical.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
• /
• v.19 no.4
• /
• pp.103-113
• /
• 2024

This study applies the UTAUT theory to examine the intention to use online shopping mall chatbot services and to elucidate the mediating effect of anthropomorphism in their relationship. For this purpose, a sample of students from A University in Gyeongnam was surveyed online using Google Docs from the first to the second week of May 2024, and after excluding 5 insincere respondents, 245 were used for the final analysis. The key findings are as follows. First, among the UTAUT factors for online shopping mall chatbot services, performance expectancy, effort expectancy, facilitating conditions, and social influence were found to have a significant positive impact on anthropomorphism. The relative impact appeared in the order of performance expectancy, social influence, facilitating conditions, and effort expectancy. Second, anthropomorphism in online shopping mall chatbot services had a significant positive impact on usage intention. Third, the UTAUT factors of performance expectancy, effort expectancy, facilitating conditions, and social influence had a significant positive impact on usage intention, with the relative impact appearing in the order of facilitating conditions, social influence, performance expectancy, and effort expectancy. Fourth, the mediating effect of anthropomorphism was confirmed in the relationship between UTAUT factors and usage intention for online shopping mall chatbot services. This study is limited to students from A University in Gyeongnam, which may restrict the generalizability of the results. Future research should select a broader sample considering regional and demographic diversity and use diverse data collection methods to enhance the reliability and validity of the data.

• Journal of Life Science
• /
• v.26 no.3
• /
• pp.282-288
• /
• 2016

Protein-protein interactions regulate the subcellular localization and function of receptors, enzymes, and cytoskeletal proteins. Proteins containing the postsynaptic density-95/disks large/zonula occludens-1 (PDZ) domain have potential to act as scaffolding proteins and play a pivotal role in various processes, such as synaptic plasticity, neural guidance, and development, as well as in the pathophysiology of many diseases. Multi-PDZ domain protein 1 (MUPP1), which has 13 PDZ domains, has a scaffolding function in the clustering of surface receptors, organization of signaling complexes, and coordination of cytoskeletal dynamics. However, the cellular function of MUPP1 has not been fully elucidated. In the present study, a yeast two-hybrid system was used to identify proteins that interacted with the N-terminal PDZ domain of MUPP1. The results revealed an interaction between MUPP1 and Wdpcp (formerly known as Fritz). Wdpcp was identified as a planar cell polarity (PCP) effector, which is known to have a role in collective cell migration and cilia formation. Wdpcp bound to the PDZ1 domain but not to other PDZ domains of MUPP1. The C-terminal end of Wdpcp was essential for the interaction with MUPP1 in the yeast two-hybrid assay. This interaction was further confirmed in a glutathione S-transferase (GST) pull-down assay. When coexpressed in HEK-293T cells, Wdpcp was coimmunoprecipitated with MUPP1. In addition, MUPP1 colocalized with Wdpcp at the same subcellular region in cells. Collectively, these results suggest that the MUPP1-Wdpcp interaction could modulate actin cytoskeleton dynamics and polarized cell migration.

• Proceedings of KOSOMES biannual meeting
• /
• 2004.05b
• /
• pp.127-150
• /
• 2004

목포해양대학교 현행 4개 학부의 교육과정은 1997년도에 전면 개편되어 학부 단위로 무난히 실시되고 있지만, 해사계열의 특수성과 해양공학계열의 학부별 전공 구성의 차이점 등으로 인하여 일률적 학부운영방침을 대학 전체에 동일하게 적용하는 것은 다소무리가 있었다. 해양계 특성화라는 대학의 목표를 향하여 각 학부의 교육목표와 전공특성을 살리면서 사회와 관련산업계의 시대적인 요구를 만족시키기 위하여 학부운영을 효율화$\cdot$극대화할 수 있는 체제 정립 방안을 연구하고 있다. 1차 년도의 연구결과에 의하면, 해상운송시스템학부는 효율적인 학부운영체제의 개선 및 운영시스템 개발을 위하여 학부 특성 및 전공 구성에 대한 분석을 통하여 문제점을 발견하고 해결방안을 모색함과 동시에 학생들의 전공 선택권을 최대한 보장할 수 있는 효율적인 학부 운영 체제를 구축하는 방안을 제시한다. 기관시스템공학부는 현행 의무복수전공제도의 문제점 분석과 해결 방안 강구를 위하여 전공들간의 인계성을 강화하며 활성화할 수 있는 방안을 모색하고 해양경찰학 전공의 운영 및 지도 방안을 연구한다. 또한 해기품질관리 관련 규정 등의 분석을 통해 교육 및 훈련에 대한 질적 향상을 꾀하고 해기품질 향상을 위한 교육평가시스템을 개발하고 구축하고자 한다. 해양전자$\cdot$통신공학부는 전공간의 연계성 구축과 효율적 운영방안을 모색하기 위하여 학부제 및 복수전공제와 관련하여 설문조사 문항을 개발$\cdot$분석하고, 해양전자공학 전공 교과목 정비 및 교재 개발을 시도하고 있다. 해양시스템공학부는 전공구성의 특성을 고려한 탐색과목의 설치 및 산업체 실습과 연계한 학점인정과목의 검토를 위하여 현행의 전공소개 프로그램을 분석하고, 졸업생의 취업을 분석하며 산업체의 요구사항을 조사하고 있다.산 알고리즘의 정당성을 보였다. 맞이하고 있음을 볼 수 있다. 국내광업이 21C 급변하는 산업환경에 적응하여 생존하기 위해서는 각종 첨단산업에서 요구하는 소량 다품종의 원료광물을 적기에 공급 할 수 있는 전문화된 기술력을 하루속히 확보해야 하며, 이를 위해 고품위의 원료광물 확보를 위한 탐사 및 개발을 적극 추진하고 가공기술의 선진화를 위해 선진국과의 기술제휴 등 자원산업 글로벌화 정책이 절실히 요구되고 있음을 알 수 있다. 또한 삶의 질을 향상시키려는 현대인의 가치관에 부합하기 위해서는 각종 소비제품의 원료를 제공하는 광업의 본래 목적 이외에도 자연환경 훼손을 최소화하며 개발 할 수밖에 없는 구조적인 어려움에 직면할 수밖에 없다. 이처럼 국내광업이 안고 있는 여러 가지 난제들을 극복하기 위해서는 업계와 정부가 합심하여 국내광업 육성의 중요성을 재인식하고 새로운 마음가짐으로 관련 정책을 수립 일관성 있게 추진해 나가야 할 것으로 보인다.의 연구 결과를 요약하면 다음과 같다. 첫째, 브랜드 이미지와 서비스 품질과의 관계에서 브랜드이미지는 서비스 품질의 선행변수가 될 수 있음을 증명하였으며 4개 요인의 이미지 중 사풍이미지를 제외한 영업 이미지, 제품 이미지, 마케팅 이미지가 서비스 품질에 영향을 미치고 있음을 알 수 있다. 둘째, 지각된 서비스 품질과 가격 수용성과의 관계에서, 서비스 품질은 최소 가격에 신뢰서비스 요인에서 정의 영향을 미치고 있으나 부가서비스, 환경서비스에서는 역의 영향을 미침을 알수 있고, 최대 가격에 있어서는 욕구서비스 요인은 정의 영향을 미치지만 부가서비스의 경우에는 역의 영향을 미치고 있음을 알 수 있다. 셋째, 서비스품질과 재 방문 의도와의 관계에 있어서 서비스품질은 재 방문 의도에 영향을 미침을 알 수 있다. 따라서 브랜드 이미지는 서비스품질의 선행변수가 될 수 있으며, 서비스품

• Journal of Korean Society of Coastal and Ocean Engineers
• /
• v.19 no.5
• /
• pp.492-501
• /
• 2007

Coastal disasters have become one of the most important issues in every coastal country. In Korea, coastal disasters such as storm surge, sea level rise and extreme weather have placed many coastal regions in danger of being exposed or damaged during subsequent storms and gradual shoreline retreat. A storm surge is an onshore gush of water associated with a tow pressure weather system, typically in typhoon season. However, it is very difficult to predict storm surge height and inundation due to the irregularity of the course and intensity of a typhoon. To provide a new scheme of typhoon damage prediction model, the scenario which changes the central pressure, the maximum wind radius, the track and the proceeding speed by corresponding previous typhoon database, was composed. The virtual typhoon scenario database was constructed with individual scenario simulation and evaluation, in which it extracted the result from the scenario database of information of the hereafter typhoon and information due to climate change. This virtual typhoon scenario database will apply damage prediction information about a typhoon. This study performed construction and analysis of the simulation system with the storm surge/coastal inundation model at Masan coastal areas, and applied method for predicting using the scenario of the storm surge.

• Tuberculosis and Respiratory Diseases
• /
• v.54 no.1
• /
• pp.80-90
• /
• 2003

Background : Goblet cell hyperplasia is a critical pathological feature in hypersecretory diseases of the airways. A bacterial infection of the lung is also known to induce inflammatory responses, which can lead to the overproduction of mucus. Recently, mucin synthesis in the airways has been reported to be regulated by neutrophilic inflammation-induced epidermal growth factor receptor (EGFR) expression and activation. In addition, it was reported that migration of the activated neutrophils is dependent on the matrix metalloproteinases (MMPs), especially MMP-9. In this study, bacterial lipopolysaccharide (LPS)-induced goblet cell hyperplasia and mucus hypersecretion by EGFR cascade, resulting from the MMPs-dependent neutrophilic inflammation were investigated in the rat airways. Methods : Pathogen-free Sprague-Dawley rats were studied in vivo. Various concentrations of LPS were instilled into the trachea in $300{\mu}{\ell}$ PBS (LPS group). Sterile PBS ($300{\mu}{\ell}$) was instilled into the trachea of the control animals (control group). The airways were examined on different days after instilling LPS. For an examination of the relationship between the LPS-induced goblet cell hyperplasia and MMPs, the animals were pretreated 3 days prior to the LPS instillation and daily thereafter with the matrix metalloproteinase inhibitor (MMPI; 20 mg/Kg/day of CMT-3; Collagenex Pharmaceuticals, USA). The neutrophilic infiltration was quantified as a number in five high power fields (HPF). The alcian blue/periodic acid-Schiff (AB/PAS) stain were performed for the mucus glycoconjugates and the immunohistochemical stains were performed for MUC5AC, EGFR and MMP-9. Their expressions were quantified by an image analysis program and were expressed by the percentage of the total bronchial epithelial area. Results : The instillation of LPS induced AB/PAS and MUC5AC staining in the airway epithelium in a time- and dose-dependent manner. Treatment with the MMPI prevented the LPS-induced goblet cell hyperplasia significantly. The instillation of LPS into the trachea induced also EGFR expression in the airway epithelium. The control airway epithelium contained few leukocytes, but the intratracheal instillation of LPS resulted in a neutrophilic recruitment. A pretreatment with MMPI prevented neutrophilic recruitment, EGFR expression, and goblet cell hyperplasia in the LPS-instilled airway epithelium. Conclusion : Matrix metalloproteinase is involved in LPS-induced mucus hypersecretion, resulting from a neutrophilic inflammation and EGFR cascade. These results suggest a potential therapeutic role of MMPI in the treatment of mucus hypersecretion that were associated with a bacterial infection of the airways.

• Korean Journal of Food Science and Technology
• /
• v.51 no.2
• /
• pp.160-168
• /
• 2019

This study aimed to determine whether double-processed ginseng berry extract (PGBC) could improve learning and memory in an $A\hat{a}42$-induced Alzheimer's mouse model. Passive avoidance test (PAT) and Morris water-maze test (MWMT) were performed after mice were treated with PGBC, followed by acetylcholine (ACh) measurement and glial fibrillary acidic protein (GFAP) detection for brain damage. Furthermore, acetylcholinesterase (AChE) activity and choline acetyltransferase (ChAT) expression were analyzed using Ellman's and qPCR assays, respectively. Results demonstrated that PGBC contained a high amount of ginsenosides (Re, Rd, and Rg3), which are responsible for the clearance of $A{\hat{a}} 42$. They also helped to significantly improve PAT and MWMT performance in the $A{\hat{a}} 42-induced$ Alzheimer's mouse model when compared to the normal group. Interestingly, ACh and ChAT were remarkably upregulated and AChE activities were significantly inhibited, suggesting PGBC to be a palliative adjuvant for treating Alzheimer's disease. Altogether, PGBC was found to play a positive role in improving cognitive abilities. Thus, it could be a new alternative solution for alleviating Alzheimer's disease symptoms.

• Asia Marketing Journal
• /
• v.13 no.3
• /
• pp.103-134
• /
• 2011

The recent economic recession and price rise reduces excessive consumption as a whole. So companies take more interest in and use discount coupons as a means of sales promotion to reinforce their competitiveness. The combination of Internet and mobile communication technology leads to an explosive increase in the use of mobile Internet service, which promotes commercialization of mobile coupons. Nevertheless, there are absolutely insufficient researches on mobile coupons than those on paper ones. In this context, this study tries to consider intention of accepting and using mobile coupons. The innovated Technology Acceptance Model (TAM) was used to see factors of using mobile coupons considered important by customers. Through the combination of characteristics of mobile coupon service and values obtained from mobile coupons, effects of variables to enhance intention of using mobile coupons were empirically analyzed. In particular, this study suggested importance of psychological as well as economic values of mobile coupons and emphasized good considerations of the psychological aspect, such as shame, stinginess, and reputation sensitivity, in using mobile coupons as an important factor for intention of using the coupons. Another empirical analysis was made of what moderating roles consumers' self-construalplayed in the effects of mobile coupon values perceived by consumers on intention of using coupons. As a result, immediate connectivity and situational provision among characteristics of mobile coupon service were found to affect ease and usability. It was also shown that perceived ease and usability had significant effects on both economic and psychological values, which then had significant effects on intention of using a mobile system. After testing moderating effects of self-construal, the degree of effects of perceived mobile coupon values on intention of using mobile coupons was greater among inter-dependent self-construal users than among independent ones. This study considered various schemes of improving intention to use mobile coupons and provided a foundation to help companies make a strategy for mobile coupons to be activated in the future.

• Journal of Aerospace System Engineering
• /
• v.14 no.5
• /
• pp.130-138
• /
• 2020

In this paper, a plan to expand the scale of the domestic MRO industry was proposed by finding the technical common points between the aviation MRO and naval vessel MRO industries. The aviation MRO industry is led by Europe, North America, and Singapore. Europe and North America have very large aviation industries. The reason for the development of the MRO industry in Singapore is that the aviation MRO and ship MRO industries gathered to expand the industrial scale. The MRO field is an industry that spans all fields from research & development, production, manufacturing, operation, disposal, and crew training. The MRO industry is divided into military and civilian use. However, most of them are only differences in the needs of users, and there are no significant technical differences. The weapon system used by the military is steadily developing. It is impossible for the military to maintain all equipment at a time when troops are reduced. For that reason, it is necessary to share roles in each field. There is a need for an MRO industry in which civil and military operations cooperate to maintain all weapon systems at optimal performance. And the MRO industry development should be based on the civil market. The scale of the MRO industry should be expanded by gathering equipment commonly used in aircraft and naval vessels. This can increase military availability and reduce maintenance budgets.