• Reproductive and Developmental Biology
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• v.29 no.1
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• pp.49-55
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• 2005

We cloned cDNA of the PGH(porcine growth hormone) gene and constructed retrovirus vector designed to express PGH gene under the regulation of CMV (cytomegalovirus) promoter. To maximize the expression, WPRE(woodchuck hepatitis virus posttranscriptional regulatory element) sequence was placed at the downstream of the PGH gene. After infection with recombinant viruses, approximately 1×10/sup 6/ PFF(porcine fetal fibroblast) cells released PGH protein into the media as much as 1,400 ng. In a subsequent experiment, a modifications of the retrovirus vector was made to express the PGH gene in a teracycline-inducible manner. In PFF cells carrying these viral vector sequences, addition of doxycycline to the media resulted in 2∼6 fold increase in PGH synthesis. In the modified retrovirus vectors, the WPRE sequence also played a role in boosting the effect of the tetracycline induction. This result indicates that our tetracycline-inducible expression system might be a promising candidate in alleviating the complicate physiological problems caused by constitutive expression of the exogenous genes in the transgenic animals.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.26 no.4
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• pp.309-316
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• 1990

Age and growth of the Marbled Sole Limanda Yokohamae in Tokyo Bay were studied by otolith reading of 1,070 fishes from May 1986 to April 1988. Examination of the outer margins of the otoliths showed that the hyaline zone as annulus was formed once a year and that its formation was completed at the end of the spawning season (Feb. to Apr.). Growth of the fish was expressed by the von Bertallinffy's equaion as Lt=255.7{1-exp[-0.505(t-0.149)]} for males and Lt=376.9{1-exp[-0.303(t-0.202)]} for females, where Lt is standard length in mm and t is age in years. It was found that the growth of males and females differs, with the females showing a higher growth than the males at each estimated age. Growth in body weight was also examined. Most of the fish examined were found to be not more than three years old, although the oldest fish were four years old for males and seven years old for females.

• Korean Journal of Ichthyology
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• v.12 no.4
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• pp.244-249
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• 2000

This study was conducted to obtain data for identification of species among elvers of anguillids, Anguilla japonica and A. anguilla and A. rostrata implanted in Korea for culture. The longer predorsal length and the fewer number of vertebrae distinguished elvers of A. rostracta from those of A. anguilla and A. japonica. A. japonica and A. anguilla were well discriminated each other in the statistical value of preanal length which the former showed shorter distance. It was remarked phylogenetically that the morphological changes according to growth in head and predorsal length of A. rostrata was different from other two species. So the elvers of A. japonica, A. anguilla and A. rostrata culturing in Korea can be distinguished by the vertebral counts, predorsal and preanal length.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.53 no.4
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• pp.544-550
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• 2020

This study was conducted to estimate the genetic improvements by selection criteria using the genetic parameters and breeding values for population of abalone Haliotis discus hannai. Genetic parameters and breeding values were estimated using all measurement data of growth traits (shell length, shell width and total weight) at 18 and 30 months old after artificial fertilization for 3,029 individuals produced in April 2014. Growth traits all exhibited moderate heritability (0.253-0.354). So it is considered that family selection will be more advantageous than individual selection. It was found that a higher genetic improvement could be expected when selecting the top 10% based on the breeding values of total weight rather than other traits. In particular, a higher genetic improvement could be expected when selecting the top 10% at 30 months old than 18 months old after artificial fertilization. This seems to be because the selection differential and heritability were higher at the 30 months old. Therefore, by estimating genetic parameters and breeding values of a population for production of the next generations by stage of growth, if they are used properly in selection and mating according to the improvement direction, it is considered that more breeding effects can be expected.

• Journal of Animal Science and Technology
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• v.47 no.2
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• pp.159-166
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• 2005

Porcine myostatin(MS1N) gene plays a key role in the differentiation of myoblast and muscle development. Genetic polymorphism was screened by single stranded conformation polymorphism(SSCP) analysis and subsequent DNA sequencing detected a nucleotide substitution(C2150T) in exon 3 of MSIN gene. Phenotypic association of the polymorphism was tested in a Landrace population and positive effects of the allele T for lean growth traits were found in the population. Even though it is not significant, the pigs have IT and TC genotypes were heavier for the body weight at birth and at twenty weeks of age than those containing genotype. Cc. However, the allele T was significantly associated with higher eye muscle area(P < 0.05). As a result of this study, we suggested that the allele T in exon 3 of MSTN gene comes a significant effect for increasing the eye muscle area without decreasing backfat thickness. This polymorphism did not change the amino acid but Taq I -RFLP matched to SSCP band patterns in exon 3 of MSTN gene, which will be an useful molecular marker for breeding of Landrace pigs.

• Journal of Embryo Transfer
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• v.18 no.3
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• pp.227-235
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• 2003

한우의 mt DNA cytochrome oxidase subunit I, II, 및 III complex지역의 유전적 다형현상을 제한효소를 이용하여 검출하였다. PCR primer 6종에 대하여 20가지 제한효소를 처리하였으며, Pst I, Pvu II, Rsa I, Eco RI, Bgl II, and Msp I 제한효소를 사용하여 유전적 변이를 검출하였다. 검출된 변이체와 한우의 성장과의 관련성을 조사한 결과 cytochrome oxidase subunit III complex 지역의 유전염기서열을 근거로 제작한 primer Mt9 좌위에서 제한효소 PvuII를 이용한 절단형과 체중형질 인 WT15(P<0.05) 및 WT18(P<0.01)에서 고도의 유의성이 관찰되었다. 아울러 , Mt9-Pvu II(P=0.07), Mt6-Bgl II(P=0.05), and Mt8-Rsa I(P=0.05) 좌위 또한 WT9, WTl5, and WT15에서 각각 통계적 유의성이 관찰되었다. 따라서 본 결과는 cytochrome oxidase subunit III complex segments가 candidate gene으로서 기초적 유전정 보 제공은 물론 유전적 개량을 위해 사용될 수 있을 것으로 사료된다.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.54 no.6
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• pp.974-981
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• 2021

The aim of this study was to evaluate genetic parameters of growth traits for improving selective breeding in olive flounder Paralichthys olivaceus produced in April 2019. The total length and body weight at 11-, 18-, and 22-months-old were measured for 7,479, 2,831 and 1,904 individuals, respectively. Since 2004, we have been conducting a selective breeding program to improve growth traits in the olive flounder. Data were analyzed using the restricted maximum likelihood method using the multiple traits animal model. The effect of sex and production period showed significant differences in all traits (P<0.05). The heritability of all traits was 0.428-0.520, which is relatively high by measurement month. Therefore, it is considered that individual selection will be more advantageous than family selection. However, to maintain an appropriate degree of inbreeding and genetic diversity for future generations, it is necessary to consider family selection adequately. Results of the correlation analysis between the same traits according to the measurement period indicated that considering production costs such as feed cost, selection at 18-months-old will be advantageous. Olive flounder is a major aquaculture species in Korea, and continuous selective breeding research is essential to improve productivity.

• Korean Journal of Ichthyology
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• v.23 no.4
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• pp.269-277
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• 2011

Age and growth pattern of Pacific cod Gadus macrocephalus were determined using samples collected in Jinhae Bay in Korea during the spawning period from 2006 to 2009. The ages of 333 specimens were estimated using their otoliths. The reliability of scales as a means of age determination was analyzed by comparing the ages estimated from otoliths and scales of 96 specimens. The scales collected from the base of the second dorsal fin or from the caudal peduncle were proved to be suitable for age determination of Pacific cod. Monthly changes in the marginal index in otoliths decreased from December showing the lowest value in February. Ages ranged from 4 to 6 years for both females and males, and most of them were 6 years old. Relationships between the otolith radius (R) and total length (TL) were TL=10.4R+3.1 for males, and TL=11.5R+3.4 for females. The growth curves in total length ($L_t$, cm) were expressed as $L_t$=141.5 [1-exp{-0.089 (t+0.209)}] for males and $L_t$=127.5 [1-exp {-0.124 (t+0.077)}] for females.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.22 no.6
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• pp.542-549
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• 2021

The purpose of this study was to provide an economical and easy preparation method for recombinant human epidermal growth factor (rhEGF) without the need for an expensive enzyme to cleave the fusion part. However, the N-terminal fusion part is still useful for affinity chromatography. The hEGF is an important hormone in cell growth and proliferation in humans, and many studies on the expression and purification of this protein have been reported. In the present study, the hEGF gene was designed to be optimized with the E. coli codon usage preference and to contain Asn-Gly at the N-terminus of the protein. The gene was inserted into pRSET_A, an E. coli expression vector, and transformed into E. coli BL21 (DE3). The recombinant fusion protein was successfully co-expressed with pG-Tf2, a chaperone vector, in E. coli and purified by Ni-NTA column chromatography. The rhEGF was then released by hydroxylamine treatment and confirmed by SDS-PAGE. ELISA analysis showed that the activity of the free rhEGF was more than 92% similar to that of commercial EGF. The biological activity of the rhEGF was confirmed by a cell proliferation test with human skin fibroblasts.

• Journal of Animal Science and Technology
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• v.48 no.1
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• pp.21-28
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• 2006

A polymorphism was found in the promoter region of porcine adipocyte fatty acid binding protein gene(A-FABP) gene which plays a key role in the binding and transportation of free fatty acid in adipocyte and deposition of intramuscular fat. Mutation was detected a substitution(T406C) using SSCP analysis and subsequently confirmed by sequencing the fragment in Duroc pigs. This T-406C mutation might change the binding activity for transcription factor nuclear factor 1(NF1). In this population, this mutation was genotyped using HinfⅠRFLP, and found three kinds of genotypes(TT, TC, and CC) showing their frequencies of 42.3, 44.3, and 13.4%, respectively. We statistically analyzed the association between the A-FABP genotypes and growth traits and found that the body weights of the pigs containing 406C/(TC or CC) were heavier for the body weight at the age of 20 weeks than those containing genotype TT(P<0.05), but not for those at the age of 0, 3, and 10 weeks. Pigs containing genotype CC had also a higher value for the average daily gain and lower values for the date for 90kg of body weight and food conversion ratio than those of 406T/- genotype. In addition, without the significant difference of back fat thickness, there was a significant association between the existence of allele CC and lean meat and eye muscle area(P<0.05). As a result of this study, we suggest that the allele T406C in the promoter region of A-FABP gene play an important role in deposition of intramuscular fat and weight in the later growth period. This polymorphism will be an useful molecular marker for breeding of Duroc pigs.