• Horticultural Science & Technology
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• v.30 no.2
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• pp.171-177
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• 2012

A total of 94 tomato accessions were evaluated for the resistance to $Tomato$ $spotted$ $wilt$ $virus$ (TSWV) using a Sw5-2 SCAR marker and bioassay. PCR products of the marker were approximately 574 bp, 500 bp, and 462 bp, among which the longest was linked to TSWV resistance allele of Sw5-b. This allele was only found in three accessions (09-438, 10-318, and 10-321) in which some individuals showed apparent recovery or stem necrosis symptom to a tomato isolate of TSWV-pb1. Thirty-five individuals (one per each accession) which were non-infected by ELISA were selected for further observation. Among these, 26 individuals that did not show any symptom at 5 months after inoculation were confirmed for viral infection by RT-PCR. TSWV-specific PCR amplicon was weakly detected in all 26 individuals including 'Eureta', a commercial F1 possessing the resistance allele of Sw5-b. The resistant genes in the selected individuals may play an important role for reducing the viral concentration in tissues of inoculated tomato plants and seems to be quantitatively controlled by several factors including Sw5-b gene.

• Resources Recycling
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• v.29 no.1
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• pp.43-52
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• 2020

The objectives of this study were to identify the optimal mix of foam concrete with the low-strength and high-flow for the repairing ground subsidence situation emergently by utilizing a large amount of industrial by-products and evaluate the possibility by applying it to the site. The factors of the experiment were the mixing ratio of mixing water and a foaming agent and the mixing ratio of foam over paste volume. The optimal mix identified by the experiment was applied to the field and basic properties were evaluated. The results of the experiment showed that the optimal mixing ratio of mixing water and the foaming agent was 10%. Moreover, when the mixing ratio of pre-foam over paste volume was 170%, it satisfied the target. However, to ensure stable quality when applying to the field, the foam mixing ratio was set 140% for the field application. The field application test of foam concrete with the low-strength and high-flow using an eco-friendly binder satisfied all target performances. Therefore, the possibility of using it as a mixture and construction method for a ground repair system is confirmed. However, there was a quality deviation between the upper part and the lower part due to the separation between foam and paste. Consequently, further studies are needed to improve it.

• The Journal of the Convergence on Culture Technology
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• v.10 no.5
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• pp.153-161
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• 2024

This study proposes improvements to the personnel management system for military officers in South Korea, addressing challenges posed by rapidly changing security environments and population decline due to low birth rates. The research identifies key issues in the current system, including lack of predictability in long-term service selection, limited opportunities for professional development, uncertainties in post-retirement careers, rigid organizational culture, and inadequate responses to demographic changes. To address these issues, the study analyzes personnel management practices in foreign militaries, including the United States, United Kingdom, and Israel, deriving valuable insights. Based on this analysis, the research suggests several improvement measures: enhancing predictability in long-term service selection, providing tailored job transition support for different military specialties, and expanding personnel exchanges between military and civilian sectors. Specific recommendations include clarifying long-term service selection criteria, introducing a phased selection system, strengthening connections between military specialties and civilian job sectors, expanding support for professional certifications, and increasing personnel exchange programs with public institutions and private companies. The study also outlines necessary legal and institutional reforms, strategies for securing and allocating budgets, and a phased implementation plan. The research proposes amendments to the Military Personnel Management Act, legislation for supporting retired military personnel, and the introduction of a performance-based budget management system. A systematic implementation plan is presented, divided into short-term, medium-term, and long-term phases.

• Research in Plant Disease
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• v.25 no.4
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• pp.220-225
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• 2019

During the year 2018, the symptoms of bunch rot on Shine Muscat (Vitis vinifera L.) were observed in Kimcheon-si, Gyeongbuk province in Korea. The disease appears on the Shine Muscat as a black rot due to prolific fungal sporulation after it has invaded into the Shine Muscat which look completely empty and dryness. Colonies of these fungi are present on the Shine Muscat skin from fruit setting and increase in amount from early season to harvest, while become peak at ripening stage. To isolate the causal agent, small fragments (2 to 3 mm) of decayed tissue from the lesion margin were placed onto potato dextrose agar (PDA) plates. Fungal colonies on PDA produced dense white aerial mycelium and then covered with dark black conidial heads. These heads were large and radiate, and vesicles were globose (2.12-32.0×2.0-3.1 ㎛). Based on morphological and cultural characteristics, this fungus was identified as Aspergillus tubingensis. To confirm its identity, the internal transcribed spacer, β-tubulin, and RNA polymerase II was sequenced for molecular identification. BLAST search indicated 99% identity with A. tubingensis. The pathogenicity test on healthy grape of Shine Muscat produced bunch rot, as the original symptoms. To select effective fungicides for the control of brunch rot, an in vitro antifungal activity of seven fungicides were evaluated against the growth of A. tubingensis. Five fungicides (dipenoconazole, tebuconazole, metconazole, iminoctadine, and captan) exhibited significantly strong suppression of the mycelial growth of A. tubingensis.

• Research in Plant Disease
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• v.12 no.3
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• pp.205-212
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• 2006

Agrobacterium vitis is a causal agent of crown-gall disease on grapevine. Bacterial strains were isolated from crown galls of different grapevine varieties in grapevine farms of Kyungbuk(Kimcheon), Chungbuk(Okcheon), Chungnam(Daejon, Choenan) and Kyeonggi(Suwon, Ansung) areas in Korea from 2002 to 2005. Primer sets, Phe A and VirA, which were derived from pectate lysase gene and virA gene of Ti-plasmid in A. vitis were used to detect A. vitis strains from crown galls. PheA and VirA primers amplified DNA fragments of 0.25 kb and 0.5 kb from fifty-one bacterialstrains. They formed crown galls on grapevine variety, Kyoho, or carrot disks with variable pathgenecity It was confirmed that the biochemical characteristics of 10 bacterial strains that was strong pathogene city on grapevine were mostly in agreement with type culture strains of A. vitis, showing growth in the presence of 2% NaCl, non-production of acid from melezitose and negative response in production of 3-Ketolactose.

• Korean Journal of Food Science and Technology
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• v.44 no.3
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• pp.280-286
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• 2012

The aim of this study was to describe the effects of genetic factors on the chemical composition or metabolites of grapes harvested within the same region. Grapes were separated into pulp, skin, and seed, and physicochemical characteristics were compared among seven grape varieties. The sugar concentrations of the grape musts ranged from $15.17-20.93^{\circ}Bx$ with Seibel variety being highest at $20.93^{\circ}Bx$. pH ranges of grape musts were 3.46-4.02 and total acidity was highest with 1.05 in Steuben variety. Tartaric acid content was highest with 146.68, 500.10 mg/L in pulp and skin extract of Merlot variety. Malic acid content was highest in pulp extract of Seibel variety (1127.14 mg/L) and skin extract of Chardonnay variety (1720.06 mg/L). K content was highest with 379.13 and 828.01 mg/L in pulp and skin extract of Chardonnay variety. Ca content was highest in pulp extract of Kyoho variety (6.98 mg/L) and skin extract of Campbell Early variety (12.26 mg/L).

• Journal of Embryo Transfer
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• v.9 no.3
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• pp.243-247
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• 1994

최근 의약적으로 유용한 단백질을 대량 생산키 위한 실현 가능한 방법이 유전자변환 가축의 이용과 관련되어 발전되어 왔다. 이러한 유전자 변환동물은 이종의 단백질을 유즙속으로 분비시키는 생체반응기로서 이용되고 있다. 이러한 전략적 목적을 위해 현재 유전자 변환동물의 생산을 위한 이용에 있어 여러 가지 방법들이 보고되고 있다. 그러나 ES 세포의 사용이 이러한 방법들 사이에서 가장 실질적인 것으로 추정되고 있다. 본 실험에서는 유전자 구축을 위해 사람 황체 호르몬(human luteinizing hormone; hLH)의 전사를 유도하기 위해 각각 2.2 및 0.5 kb의 토끼 $\beta$-casein pronoter 단편을 이용하여 생쥐의 유선에 hLH를 발현시키도록 조절하고 발현이 thynidine kinase(TK) pronoter에 의해 좌우되는 neo 유전자를 selectable marker로서 plasnid속에 삽입하였다. 그 결과 생긴 구축 유전자는 각각 pCas 2.2와 pCas 0.5로 명명하였다. 구축된 유전자로 2$\times$107의 TT-2 ES세포를 170V, 550$\mu$F로 100$\mu$g의 선상 plasmid에 의해 electroporation 시켰다. 감염된 colony들은 250$\mu$g/$m\ell$ G418을 함유하는 ESM 배양액에서 선별 7일 이후에 회수하여 성공적으로 감염된 ES세포는 PCR 및 Southern blot에 의해 확인되었고 그들 중 나머지는 trypsin 처리 후 각각 미세조작과 공배양 기술을 사용하여 ICR 생쥐의 8세포기 수정란 속에 도입하였다. 결국 24시간 동안 37$^{\circ}C$, 5% $CO_2$에서 배양된 배반포를 chimera의 생산을 위해 위임신 유기된 G418 선발처리 이후 400 및 275개의 ES 세포 colony가 생존하였으며, 3개의 ES 세포으 colony 의 genome 속에 임의적으로 plamid가 삽입된 것을 Southern blot에 의해 확인되었다. 총 13 chimera 생쥐가 3 colony로부터 생산되었으나 germ-line chimera는 현재 조사중이다. chimera 생산빈도는 공배양 기술보다 주입방법에서 현저히 높았다.

• 한국벤처창업학회:학술대회논문집
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• 2016.04a
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• pp.67-69
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• 2016

이 연구는 경영학과 MBA교육과정이 세상의 모든 제품과 기업에 공통적으로 적용되듯이, 세상의 어떤 분야의 제품이든 그 시작인 한 개 착안에서부터 시장력 확보를 위해 시도 되는 도전행위의 매개인 모든 착안과 아이디어가 기업이 되는 과정인 제벤처행위에 공통적으로 적용되는 학문이 될, MVA(PH-MVA, master of venture arming, 벤처무장학)학의 개념적 모델링의 시작에 필요로하는 정의론과 개념론으로, MBA에 있어 경영학원론이 개론화되듯, MVA에 있어 그 개론서가 될 벤처학원론서 설계에 있어서의 시작인 1편 기초론을 구성하는 내용을 벤처창업이나 기업의 신제품개발 부서에의 취업을 희망하는 학생들과 제벤처 생태계의 모든 직간접 관련 직무자 들이 공통적으로 무장하면 벤처적 행위에 필요로하는 여러 가지 직무자들 같이 소통력은 물론 직무매칭력 등의 분업 협업력이 매우 높아 질 것이라는 주장을 바탕으로한다. 그리고 제시하는 정의와 개념을 제벤처 생태계 전체의 표준으로 참고 삼아, 누구의 어떤 모델이든, 창조경제의 일부로서 미생들의 벤처(창업, 기업만들기 도전)행위나 완생기업들의 신제품개발 모두에 두루 적용 될 수 있는, 누구나가 이해, 공감, 교감, 습득이 용이한 벤처학 개발이 왕성하게 활성화될 수 있도록 하는 것이 곧 창조경제 활성화를 위한 학자들의 사명이자 새로운 성장동력 모델의 발굴이기도하다는 측면에서의 동참이 될 수 있기를 바라는 마음이다. 아울러, 이러한 정의와 개념을 바탕으로 전개되어, 벤처생태계 전반에서 수행 될 교육이 개인들의 창업(기업만들기)을 목표로하는 공부가 되기도하고 연장에, 완생기업들이 필요로하는 신제품개발에 있어서의 기본적인 능력과 자질을 갖춘 인재선발적 취업준비공부도 될 수 있는 통합적 교육이되어야한다는 취지를 가지고있다. 또 연구를 통해 제벤처(기업의 신제품개발 도전협업행위 포함) 생태계에서의 직무자들의 직무매칭력 및 협업행위에 있어서의 다양한 용어와 정의와 개념들의 제각각으로 부터 발생되는 상치(相馳, 제각각, 불통, 협업마찰, 대립, 갈등, 등)력을 해소하는 것이 역설적으로 협업력을 최적으로 활성화 할 수 있다는 사실을 모두가 인지, 수용토록 하고자 함을 목적으로한다. 이러한 연구를 전벤처단계의 벤처기초학을 바탕으로하는 제벤처생태계 개념을 일원화 통합화하는 연장에 전개되는 공통무장교육 모델화를 목적으로한다.

• Korean journal of applied entomology
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• v.35 no.1
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• pp.66-73
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• 1996

For the effective control of mosquito larvae, Anopheles sinensis, the expression vector pCYASK5-1 containing cryIVD gene of Bacillus thuringiensis subsp. morrisoni PG-14 was constructed and transformed into the cyanobacterium Synechocystis PCC6803. The transformants were selected on BG-11 medium containing kanamycin. The expression of cryIVD gene in transformant was confirmed by SDS-PAGE and Western blot analysis. The mortality of A. sinensis larvae was scored for 3 days. Furthermore, growth and distribution rate of transformant were examined. The results showed that Synechocystis PCC6803 transformed with cryIVD gene of B. thuringiensis subsp. morrisoni PG-14 was highly toxic to A. sinensis larvae, demonstrating that it will be a potential agent for mosquito control.

• Journal of Plant Biotechnology
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• v.47 no.3
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• pp.227-234
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• 2020

To produce the miraculin protein in suspension cultures, rice (Oryza sativa L.) was transformed with Agrobacterium tumefacience EHA105 containing the miraculin AB512278 gene. The cell suspension cultures were established using cell lines selected from transgenic rice callus. The integration of the miraculin gene into the rice chromosome was confirmed using genomic PCR analysis. In addition, RT-PCR analysis indicated that the miraculin gene is expressed in the selected suspension cell lines. Thus, the recombinant miraculin was expressed in the transgenic suspension cell line, HK-2. Therefore, we have successfully developed a HK-2 line that produces miraculin. These results demonstrate that transformed cell suspension cultures can be used to produce a taste-modifying protein such as miraculin.