• Clean Technology
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• v.17 no.3
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• pp.231-237
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• 2011

Two-photon microscopy (TPM) is attracting much attention in biological imaging due to the capability of imaging deep inside the living tissues for a long period of time. For maximum utilization of TPM, it is essential to develop efficient twophoton sensors. Regarding this, many research groups are developing two-photon sensors for specific applications. In this review, we summarize recent results on selected examples of two-photon sensors for intracellular free metal ions in the live cells and tissues to provide a guideline for various imaging applications.

• The Journal of the Korea institute of electronic communication sciences
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• v.9 no.3
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• pp.323-328
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• 2014

Effect of chitosan with sirloin specimens from chitosan-fed and normal-fed pig. The specimens were observed by a optical microscope and their biophoton was measured by a photomultiplier(PMT)tube. Results of experiments showed that specimens of the chitosan-fed pig had lower biophoton rate than that of the normal-fed pig. As a result of the organization were also shown pictures Chitosan as a pork loin tissue and fat in the flesh less delicate and compactness is found that tastes good. Thus by PMT fleshy tissue biochemical measurements to measure the change of state to be one of the new method is to be expected.

• Proceedings of the Optical Society of Korea Conference
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• 2009.10a
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• pp.235-236
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• 2009

파장이 길고 에너지가 낮은 근적외선 영역 광자 두 개를 동시에 여기했다가 형광으로 방출하는 비선형 현상을 이용한 이광자 현미경은 살아있는 세포를 넘어 생체조직 깊은 곳에서 일어나는 생물학적 변화 관찰 및 3차원 이미지 해석이 가능한 매우 효과적인 장비이다. 그러나 상용화된 이광자 현미경은 매우 고가이고 응용의 다양성을 위한 구조적 변형이 매우 힘들다. 본 논문에서는 모드잠김 이터븀 광섬유 레이저를 이용하여 비용 효율이 높고 다루기 쉬운 이광자 현미경을 구현 하고자 한다.

• Optical Science and Technology
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• v.17 no.3
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• pp.5-8
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• 2013

• Journal of Biomedical Engineering Research
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• v.12 no.1
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• pp.43-48
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• 1991

As the computerized methods and equipments In nuclear medicine imaging increases, quantitative information is needed on the single photon emission computed tomographic Images as well as on the conventional nuclear medicine images. In this paper, the authors investigated the effect of several clinician - friendly reconstrution filters on the resultant transverse slices of backprojected Profiles of radioisotope distribution from the Quantitative point of view, and reduced the filter parameters such as cutoff frequency and order of filter which are neces mary to minimize the quantification error using computer-generated phantoms.

• KSBB Journal
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• v.19 no.1
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• pp.12-16
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• 2004

Recently the measurement of biophoton emission has attracted increasing attention in the study on physiological state of biological systems. We report the measurements of biophoton emission from the mouse fatty liver induced by carbon tetrachloride, CCl$_4$. The hepatotoxin, CCl$_4$ in olive oil, was injected intraperitoneally into two groups of ICR mice which were made of 6 mice in each group. The control groups corresponding to the treated groups were prepared with the injections of olive oil only. After the injections, livers of two groups were extracted and measured biophoton emission in 24 hours and 72 hours later, respectively. We also extracted the plasma in the blood and measured the transaminase activity. Results show that biophoton emission from the livers in 24-hour treated group is 69.3${\pm}$21.2 counts/min/$\textrm{cm}^2$, which is two times more larger than that in 24-hour control group, 29.5${\pm}$5.9 counts/min/$\textrm{cm}^2$ Biophoton emission from the livers in 72-hour treated group is 37.0${\pm}$14.8 counts/min/$\textrm{cm}^2$. These biophoton results correlate with those of the biochemical assays. We conclude that biophoton emission can be used as a biomarker of mouse fatty liver induced by CCl$_4$.

• KSBB Journal
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• v.18 no.3
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• pp.170-173
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• 2003

This study was studies biophoton characteristics of Madin-Darby canine kidney cells under the influence of CsA and each cell type (mock, wt, R55A) by employing a Photomultipliertube. $\textrm{H}_2\textrm{O}_2$ was used for producing reactive oxygen species (ROS) in this measurement. ROS is also generated during oxidative metabolism in living organism. Images from a fluorescence show an increase of photon intensity emitted from the sample on the influence of CsA and each cell type (mock, wt, R55A). It is believed chemiluminescence (CL) occurred by ROS is responsible for the biophoton emission. hence PMT measurement might be considered as a useful tool for studying biochemical characteristics in relation to ROS.

• Proceedings of the Materials Research Society of Korea Conference
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• 1993.05a
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• pp.61-61
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• 1993

20세기 막바지에 들어 서면서 21세기를 바라보는 정보화사회는 어떠한 과학기술의발전을 필요로 하는 것일까? $10^{12}$ 집적도, $10^{-8}$cm 원자공간, $10^{-12}$-$10^{-15}$ 초등 시간대역에 대한 도전과[ 테라바이트 메모리], [테라바이트 컴퓨터], [테라비트 광통신] 등을 앞세우면서 고속화, 대용량화, 초미세화, 다기능화, 고기능화, 지능화를 지향하는 미래 정보통신 기술 실용화를 위하여서는 어떠한 성질의 신소재들이 개발되어야 할 것인가? [20세기 전자시대] 의 대표적 정보운반자인 [전자] 에 대한 연구는 21세기에는 어떻게 전개될 것인가? 새로운 정보운반자로서 부상하고 있는 [광자],[뉴런],[생체분자]등에 대한 연구는 어떠한 방향으로 전개될 것인가? 신개념의 정보통신 기술을 구체적으로 실용화 하기 위하여 연구되어야 할 신소재는 어떻게 전개되어야 할 것인가\ulcorner 초미세구조, 양자효과, 비선형효과, 원자가공, 원자조작, 인공신소재, 초격자 지능신소재, 초전도 유기물, 분자, 광논리, 광신경망, 생체노리, 생체컴퓨터등 신개념의 창출로부터 비롯해서 의료, 복지, 장애. 기후. 환경. 지각.해양. 항공. 우주에 이르는 다차원적 통신과 지능형 정보를 가능케 하는 신소재 연구의 조건들과 그에 따른 도전을 전망해 본다.에 따른 도전을 전망해 본다.

• Journal of Biomedical Engineering Research
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• v.18 no.4
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• pp.499-505
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• 1997

A non-invasive technique to measure absorption and scattering coefficients was investigated The reflected backscattered light from the surface of phantom and biological tissue was obtained by using a time-correlated single photon counting system in pico-second time domain. The absorption and scattering coefficients were acquired by the time of peak and asymptotic behavior of the time-resolved reflectance curve and agreed well the ones that is obtained with deconvolution method It was found that the approximation method was good for biological medium to calculate optical properties due to its convenience and accuracy.

• Journal of Radiation Protection and Research
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• v.36 no.2
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• pp.86-92
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• 2011

This study used the optically stimulated luminescence dosimeters (OSLDs), recently, received the revaluation of usefulness in vivo dosimetry, and the diode detecters to measure the skin dose of patient with the rectal cancer. The measurements of dose delivered were compared with the planned dose from the treatment planning system (TPS). We evaluated the clinical application of OSDs in radiotherapy. We measured the calibration factor of OSLDs and used the percent depth dose to verified, also, we created the three point of surface by ten patients of rectal cancer to measured. The calibration factors of OSLD was 1.17 for 6 MV X-ray and 1.28 for 10 MV X-ray, demonstrating the energy dependency of X-ray beams. Comparison of surface dose measurement using the OSLDs and diode detectors with the planned dose from the TPS, The skin dose of patient was increased 1.16 ~ 2.83% for diode detectors, 1.36 ~ 2.17% for OSLDs. Especially, the difference between planned dose and the delivery dose was increased in the perineum, a skin of intense flexure region, and the OSLDs as a result of close spacing of measuring a variate showed a steady dose verification than the diode detecters. Therefore, on behalf of the ionization chamber and diode detecters, OSLDs could be applied clinically in the verification of radiation dose error and in vivo dosimety. The research on the dose verification of the rectal cancer in the around perineal, a surface of intense flexure region, suggest continue to be.