• Korean Journal of Microbiology
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• v.51 no.2
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• pp.97-107
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• 2015

Wetlands constitute a transitional zone between terrestrial and aquatic ecosystems and have unique characteristics such as frequent inundation, inflow of nutrients from terrestrial ecosystems, presence of plants adapted to grow in water, and soil that is occasionally oxygen deficient due to saturation. These characteristics and the presence of vegetation determine physical and chemical properties that affect decomposition rates of organic matter (OM). Decomposition of OM is associated with activities of various extracellular enzymes (EE) produced by bacteria and fungi. Extracellular enzymes convert macromolecules to simple compounds such as labile organic carbon (C), nitrogen (N), phosphorus (P), and sulfur (S) that can be easily taken up by microbes and plants. Therefore, the enzymatic approach is helpful to understand the decomposition rates of OM and nutrient cycling in wetland soils. This paper reviews the physical and biogeochemical factors that regulate extracellular enzyme activities (EEa) in wetland soils, including those of ${\beta}$-glucosidase, ${\beta}$-N-acetylglucosaminidase, phosphatase, arylsulfatase, and phenol oxidase that decompose organic matter and release C, N, P, and S nutrients for microbial and plant growths. Effects of pH, water table, and particle size of OM on EEa were not significantly different among sites, whereas the influence of temperature on EEa varied depending on microbial acclimation to extreme temperatures. Addition of C, N, or P affected EEa differently depending on the nutrient state, C:N ratio, limiting factors, and types of enzymes of wetland soils. Substrate quality influenced EEa more significantly than did other factors. Also, drainage of wetland and increased temperature due to global climate change can stimulate phenol oxidase activity, and anthropogenic N deposition can enhance the hydrolytic EEa; these effects increase OM decomposition rates and emissions of $CO_2$ and $CH_4$ from wetland systems. The researches on the relationship between microbial structures and EE functions, and environmental factors controlling EEa can be helpful to manipulate wetland ecosystems for treating pollutants and to monitor wetland ecosystem services.

• Journal of Wetlands Research
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• v.15 no.3
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• pp.289-300
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• 2013

In this study, two types of wetland plants, Eichhornia crassipes (a floating plant) and Ceratophyllum demersum (a submerged plant) were introduced to wetland mesocosms to understand how the water properties of wetlands such as pH, dissolved oxygen content, water temperature, oxidation reduction potential, and nutrient concentrations are affected by different types of wetland plant. The floating plant lives on the water surface and can block light penetration; it exhibited the lowest water temperature and temperature difference between lower and upper layers. After the addition of contaminants, the dissolved oxygen (DO) concentration decreased abruptly but recovered continuously in all mesocosms; especially the submerged plants, which photosynthesize in water, showed the largest increases in DO and diel periodicity DO, as well as in pH value. The oxidation-reduction potential in both water and sediment were affected by the presence of wetland plants and plant type and the results suggest that various aspects of wetland biogeochemistry are affected by the presence and type of wetland plants. The total nitrogen and phosphorous concentrations in water decreased in the following order: Water only < Water + Soil < Floating Plants < Submerged Plants. Although both floating and submerged plants can control algal concentrations, the effect was more prominent for floating plants.

• Journal of the Korean earth science society
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• v.32 no.2
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• pp.170-179
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• 2011

Fly ashes derived from coal fired power plants have unique chemical and mineralogical characteristics. The objective of this research was to study how indigenous bacteria affected heavy metal leaching in fly ash slurry during the fly ash-seawater interactions in the ash pond located in Yeongdong seashore, Korea. The in-situ pH of ash pond seawater was 6.3-8.5. For this study, three sites of the ash pond were chosen to collect a sample of fly ash slurry. Three samples that had a mix of fly ash (0.4 L) and seawater (1.6 L) were collected at each site. First sample was autoclaved ($120^{\circ}C$, 2.5 atm), second one was inoculated with glucose to stimulate the microbial activity, and the last sample was kept in the natural condition. Compared with other samples including autoclaved and natural samples, the glucose added sample showed sharp increase in its alkalinity after 15 days, cation concentration change such as Ca, Mg, and K seemed to increase in early stage, and then decrease 15 days later in slurry solution of glucose added sample, and a possibly considerable decrease in $SO_4^{2-}$ in the fly ash slurry samples when glucose was added to stimulate the microbial activity. Geochemical data of this study is likely to be related to the activity of bacteria at the ash pond. The result may be used to understand about the characteristic of bacteria.

• Korean Journal of Ecology and Environment
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• v.40 no.1
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• pp.93-102
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• 2007

The runoff characteristics of organic matter in turbid water were investigated in eleven tributary streams of the Han River system, Korea. The flow-weighted event mean concentrations of organic matter ranged from 1.5 to 3.2 mg $L^{-1}$ of DOM and 2.2 of 29.1 mg $L^{-1}$ of POM, respectively. The SUVA value which reflects the proportion of humic substance in organic matters was higher during the rainfall season, meaning that the runoff of refractory form increase in this period. Stable carbon isotope ratios of both POM and DOM were different among streams, which reflect the sources of organic matter. DOM isotope ratios were less depleted of $^{13}C$ than that of POM by approximately 1 to $2%_{\circ}$ ${\delta}^{13}C$ of the several turbid streams (the Mandae Stream, the Jawoon Stream, and the Daegi stream) were heavier than those of clear streams. ${\delta}^{13}C$ values in the turbid upstream tributaries were similar to those of downstream reaches (such as the Soyang River, the Sum River, and the Seo River). From the ${\delta}^{13}C$ analysis of POM it could be calculated that $C_4$ pathway contributed approximately 15.9 to 23.6% of organic matter in several turbid upstream sites, and over 20% in the three sites of large downstream reaches. On the contrary it contributed only 9.1 to 12.8% in clear streams of forest watersheds. In the Soyang River, $C_4$ pathway organic matter contributed 8.8% of the DOM pool.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.19 no.2
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• pp.147-154
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• 2014

Bacteria play an important role in biogeochemical cycles in marine environments and their functional attributes in ecosystems depend primarily on species composition. In this study, seasonal variation of bacterial diversity was investigated by pyrosequencing of 16S rDNA in surface sediment and overlying seawater collected in the south East Sea, planned for the site of $CO_2$ sequestration by the carbon capture and storage (CCS) project. Gammaproteobacteria was dominant in the sediment in most seasons, whereas Alphaproteobacteria was a most dominant group in the overlying water. Thus, the bacterial diversity greatly differ between sediment and seawater samples. On the genus level, bacterial diversity between two habitats was also different. However, the number of genera found over 5% were less than 10 in both habitats and the bacterial community was composed of a number of diverse minor or rare genera. Elevation of $CO_2$ concentration during a $CO_2$ storage process, could result in change of bacterial diversity. Thus, this study will be very useful to access the effect of $CO_2$ on bacterial diversity and to predict functional change of the ecosystem during the process of CCS project.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.9 no.4
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• pp.179-195
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• 2004

The trophic state of the coastal waters of the southern part of Korea was assessed using biogeochemical data obtained from the National Marine Environmental Monitoring Program conducted by the National Fisheries Research and Development Institute for six years. The trophic state of different areas, analyzed by non-metric multi-dimensional scaling (MDS) analysis, could divide the areas into three groups. Masan Bay, with suboxic water masses and/or the highest concentrations of dissolved inorganic nitrogen and phosphorus occurred, was assessed as being in a hypertrophic state. Ulsan Bay, Onsan Bay, Busan and Jinhae Bay, located near strong point sources, were in a eutrophic state. Other areas, including Tongyong, Yosu, Mokpo and Jeju island, were evaluated as being in a mesotrophic state. During 1997 to 2002, the average values of excess nitrogen, which is the difference between the measured dissolved inorganic nitrogen (DIN) and the corrected DIN using the Redfield ratio, were positive at Ulsan, Onsan, and Busan, where there were inflows from polluted rivers. In contrast, those were negative values in Haengam Bay, Gwangyang Bay and nearby Yosu. This suggests that the limiting element for phytoplankton growth differed among sites. The time series data of excess nitrogen showed gradual decrease over time in the hypertrophic waters, but the opposite trend in the mesotrophic waters. This indicated that the ratio of nitrogen to phosphate varied according to the trophic state of the coastal waters. The enrichment of organic matter in sediment in eutrophic waters would disturb the normal pattern of biogeochemical cycling of nitrogen and phosphate. In order to assess the condition of the coastal environment, the benthic boundary layer should be considered.

• Economic and Environmental Geology
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• v.47 no.1
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• pp.61-69
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• 2014

As we are trying to in-situ treat (purify or immobilize) heavy metals or radionuclides in groundwater, one of the geochemical factors to be necessarily considered is the value of oxidation/reduction potential (ORP) of the groundwater. A biogeochemical impact on the characteristic ORP change of groundwater taken from the KAERI underground was observed as a function of time by adding electron-donor (lactate), electron-acceptor (sulfate), and indigenous bacteria in a laboratory condition. There was a slight increase of Eh (slow oxidation) of the pure groundwater with time under a $N_2$-filled glove-box. However, most of groundwaters that contained lactate, sulfate or bacteria showed Eh decrease (reduction) characteristics. In particular, when 'Baculatum', a local indigenous sulfate-reducing bacterium, was injected into the KAERI groundwater, it turned to become a highly-reduced one having a decreased Eh to around -500 mV. Although the sulfate-reducing bacterium thus has much greater ability to reduce groundwater than other metal-reducing bacteria, it surely necessitated some dissolved ferrous-sulfate and finally generated sulfide minerals (e.g., mackinawite), which made a prediction for subsequent reactions difficult. As a result, the ORP of groundwater was largely affected even by a slight injection of nutrient without bacteria, indicating that oxidation state, solubility and sorption characteristics of dissolved contaminants, which are affected by the ORP, could be changed and controlled through in-situ biostimulation method.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.3 no.4
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• pp.203-213
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• 1998

To investigate impacts of waste disposal on the marine environment for 9 years since dumping activity commenced in the Yellow Sea, chemical analysis of seawater has been done in and around the ocean dumping site. Results show that biogeochemical changes have occurred locally in the dumping area compared with the reference area, resulting in low dissolved oxygen saturation, low pH, and high accumulation of nutrients in the bottom layer. According to cluster analysis, chemical environment of the study area can be largely divided into surface layer and bottom layer, and again the bottom layer can be divided into that of dumping area where nutrients are accumulated and that of reference area. Low pH, low dissolved oxygen, high COD and accumulation of nutrients mainly characterize the dumping area. Average concentration of total inorganic nitrogen in the bottom layer below the thermocline has increased from $3.7{\mu}M$ to $9.5{\mu}M$, 250% accumulation in the dumping area since 1988. Therefore, the present results clearly show that the dumping site of Yellow Sea has received excessive sewage input beyond the environmental assimilatory capacity during the last decade. It may result in serious aggravation of seawater quality and basic ecosystem, and devastation of overall fishing grounds of Yellow Sea in the near future.

• Proceedings of the Korea Water Resources Association Conference
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• 2023.05a
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• pp.223-223
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• 2023

토양수분은 지표면과 지하 영역 사이에 존재하는 수분 및 열에너지의 분배를 제어하거나, 토양 영양분, 식물 성장 및 미생물 활동과 같은 다양한 환경 과정에 영향을 미치는 핵심 구성요소이다. 토양수분은 생태수문학 및 생지화학적 역학, 저수지 관리, 가뭄 및 홍수의 경고, 토양 수분 변화에 따른 작물 수확량 등을 이해하는 데 매우 중요한 역할을 한다. 따라서, 토양 수분의 정확한 측정은 필수적이며, 이러한 필요성에 따라 중력 측정법, 장력 측정법, 전기 저항법 및 시간-주파수 영역반사측정법 등의 다양한 측정 방법들이 다년간 개발되어 사용되었다. 다만, 앞선 방법들은 철저한 실험을 통해 높은 정확성을 확보하였지만, 토양 교란이 발생하는 단점이 존재하며 실험 현장 토양의 물리적, 생물학적, 그리고 화학적 특성의 보존은 매우 어려운 한계점을 가지고 있다. 따라서, 이러한 단점을 극복하기 위해, 본 연구에서는 레일리파를 이용한 비접촉식 비교란 토양수분 센서 개발을 목표로 한다. 모래, 실트, 점토와 같은 세 가지 특징적인 토양 유형에 따른 파동을 측정하고, 측정된 파동으로부터 토양 수분을 추정하기 위해 기존에 개발된 시간-주파수 방법을 활용하여 토양수분을 함께 측정하였다. 비접촉 파동신호를 토양수분으로 변환하기 위하여, fully convolutional network을 개발하였다. 개발한 모델의 결과 검증은 RMSE(Root Mean Square Error)를 활용하여 검증하였으며, 모래, 실트, 점토에서 각각 0.0131, 0.0021, 0.0034 m³ m^-3으로 상대적으로 높은 정확성을 보였다. 즉, 본 연구에서 제시한 누출 레일리파를 사용한 비교란-비접촉 토양수분 측정 방법으로, 토양을 교란하지 않고 토양수분을 측정 할 수 있는 높은 가능성을 제시하였다.

• Proceedings of the Korea Water Resources Association Conference
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• 2023.05a
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• pp.434-434
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• 2023

다양한 지면 모형은 대기 강제력 데이터 세트에 의해 구동되며 육지의 물, 에너지 및 생지화학적 순환의 해석에 활용된다. 그 중 에너지 플럭스 교환을 추정하는 것은 극심한 가뭄, 폭염, 물 부족 등 극한 기후 현상에서 중요한 역할을 한다. 에너지 플럭스는 기상기후조건과 토지피복의 변화에 따른 영향을 받고 있는데 그 영향을 구체적으로 조사하는 것은 생태계 프로세스의 매커니즘을 구성하는 데 필수적이다. 본 연구에서는 최신버전인 Community Land Model 버전 5.0 (CLM5)를 이용하여 동북아시아 지역의 에너지 플럭스의 시공간분포를 분석하였다. CLM5의 시뮬레이션은 1991년부터 2010년까지 2.5° × 2.5° 그리드에서 실행되었고 주요 에너지 인자인 순복사량, 현열, 잠열을 모의하였으며, 실행결과는 FLUXNET의 동북아시아 사이트의 관측자료를 이용하여 모델을 검증 및 평가하였다. 대기 강제력 변수의 차이는 모의 결과에 영향을 미치기 때문에 수문인자와 토지피복유형에 따른 에너지 플럭스의 변동성을 분석하였고 잠열을 식생 증발산열과 지면 증발열로 파티션하여 연구지역에 따른 각 구성요소의 비율을 산정하였다. 20년간의 순복사열, 잠열과 온도의 시공간적 변동성의 연 추세를 분석한 결과 동북아시아의 대부분 지역에서 잠열과 온도는 소폭 증가되였고 순복사열은 중국 내륙과 몽골지역에서 감소되였다. 본 연구는 지표와 대기 사이의 에너지 교환에 대해 분석하였으며 이후 증발산 및 물 플럭스와의 연동성과 관계성 분석에 활용하여 기후변화를 이해하는 데 기여할수 있을 것으로 사료된다.