• The Journal of Natural Sciences
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• v.14 no.2
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• pp.103-114
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• 2004

This study aims at investigating the effect of soil moisture control and truss limited high density culture on the growth, fruit yield and quality of tomato. To minimize of loss yield of tomato, flower cluster in number was limited to two and three truss and planting density was raised. Soil moisture control was started from 40 days after anthesis and irrigation point was set in -30kPa and -50kPa, which were compared with -10kPa For high density culture, the planting number of truss limited high density culture was planted twice as many as control. Soil moisture repression reduced the growth of stem diameter, leaf and plant height. Leaf chlorophyll content was higher in -50kPa and -30kPa than control. No significant differences, however, shows in -10kPa. The occurrence rate of bloom-end rot and cracking was increased by growing of irrigation repression. Pinching three fruit truss was higher than pinching two fruit truss in the occurrence rate of them. Soil moisture repression resulted in the reduction of fruit weight and in special, truss limited high density was distinctly decreased in -50kPa. The number of fruit was not affected by soil moisture control, but 3rd flower cluster was lower than 2nd flower cluster in the number of fruits and 2nd one was lower than 1st one. Under irrigation repression, rate of dry matter tended to grow in -30kPa, -50kPa compared with control and pinching three fruit truss was higher than two truss. Marketable yield dropped to 36.7%m 46.3 in -30kPa, -50kPa on pinching two fruit truss and dropped to 27.3%, 32.3% in 3rd flower cluster compared with control.

• Horticultural Science & Technology
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• v.19 no.4
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• pp.495-500
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• 2001

Seed germination, seedling growth, plant establishment after transplanting, set of female flowers, and subsequent fruit growth were examined in cucumbers grown in soils having varying electrical conductivities (EC) of $1.0-5.0dS{\cdot}m^{-1}$ adjusted by adding different amount of a commercial compound fertilizer (N : $P_2O_5$ : $K_2O$ = 21 : 17 : 17). Slower seed germination was recorded in soils having EC value of $3.0dS{\cdot}m^{-1}$ or higher and little or no germination took place in soils with EC $4.0dS{\cdot}m^{-1}$ until 8 days after sowing. Suppression of seedling growth was apparent in soils having EC level of $2.5dS{\cdot}m^{-1}$. All seedlings died eventually at EC level of $3.5dS{\cdot}m^{-1}$. Leaf color became progressively dark green with the increase in EC from 1.0 to $3.0dS{\cdot}m^{-1}$. The first female flower was formed at higher node and the number of days required for the first flowering of female flower increased with the increase in EC values up to $3.0dS{\cdot}m^{-1}$. Occasionally, leaf yellowing and self-topping symptoms appeared in plants grown in soils having EC level of $3.0dS{\cdot}m^{-1}$.

• Horticultural Science & Technology
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• v.28 no.4
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• pp.552-560
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• 2010

$GA_{4+7}$+BA, a plant growth regulator for induction of feathering in young apple tree and increasing fruit size, was applied by various methods on 'Gala'/M.9 apple trees in high density orchard for 4 years to investigate its effect on fruit and shoot growth. $GA_{4+7}$+BA($80-300mg{\cdot}L^{-1}$) increased fruit length, fruit weight, and L/D ratio regardless of application methods, but it did not affect soluble solid content, acidity, leaf area, and chlorophyll. Seed number was not affected by $GA_{4+7}$+BA application, however, more immature seeds was observed in treated 'Gala' fruit. Shoot growth was increased when spraying $GA_{4+7}$+BA at tree crown but not affected when spraying at fruit directly. More significant fruit growth was observed when $GA_{4+7}$+BA was applied on the fruits between late of May and early of June when fruit cell division ended; however, high concentration of $GA_{4+7}$+BA resulted in lower fruit storability because of lower firmness. Hence, more attention should be paid when applying high concentration of $GA_{4+7}$+BA to small sized fruit cultivars like 'Gala'.

• Korean Journal of Plant Resources
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• v.24 no.4
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• pp.461-471
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• 2011

This study was carried out to establish an in vitro culture method of callus having a high antioxidant activity from Ginkgo biloba L. Leaf explants were cultured on Murashige and Skoog's medium supplemented with various growth regulators. The explants were incubated in the dark or 3,000 lux cool-white light. Methanol extracts from incubated callus were evaluated for scavenging activity of the free radicals using DPPH. The best callus growth rate was achieved in MS medium combined with 10 ${\mu}M$ NAA and 5 ${\mu}M$ kinetin in the light condition. Total antioxidant activity of cell aggregates in suspension culture [MS medium supplemented with 10 ${\mu}M$ NAA in the light] was up to 80% of ascorbic acid. By means of HPLC analysis, quantification of the quercetin dehydrate and keamperol profiles from suspension callus was compared. Contents of quercetin dehydrate and keamperol from leaf extracts were 0.07 and 2.24 ${\mu}g/20{\mu}l$, and those from callus 0.56 and 0.18 ${\mu}g/20{\mu}l$, respectively.

• Korean Journal of Plant Tissue Culture
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• v.22 no.5
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• pp.283-290
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• 1995

This experiment was canted out to obtain embryogenic callus and to understand developmental mechanism of somatic embryogenesis in Oenanthe javanica ($B_{L.}$) DC. experiments included the examination of explant source and media for embryogenic callus production and the observation of developmental pattern of embryogenic cells and non-embryogenic cells. Embryogenic calli were formed on zygotic pro-embryos together with their endosperms when they were cultured on Ms media containing 1.0mg/L 2,4-D. Embryogenic calli were also formed on the intact surface in vitro grown stem or petiole segmentsafrer 6-8 weeks of culture, whereas non-embryogenic calli were formed on cut surfaces of the stem and petiole after 2 weeks of culture. Non-embryogenic calli were rhizogenic in suspension and solid media culture.

• Korean Journal of Plant Tissue Culture
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• v.21 no.1
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• pp.1-6
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• 1994

Cotyledonary and hypocotyl explants of melon seedlings were cultured on Murashige and Skoog's (MS) medium supplemented with various concentrations of 2,4-Dichlorophenoxyacetic acid (2,4-D) and benzyladenine (B.A).Up to 22% of cotyledonary explants and 7%, of hypocotyl explants, respectively: Produced somatic embryos through intervening two types of calli: bright yellow compact (BYC) callus and pale-yellow compact (PYC) callus. BYC callus was capable of producing somatic embryos at initial culture, but it became necrotic as subrulhues proceeded. In contrast UC callus was incapable of producing somatic embryos during initial culture (first 6 weeks), but it became bright-yellow friable (BYF) callus with forming a few globular embryos after 2 months of subculture, indicating that the callus turned embryogenic. The embryogenic capacity of BYF maintained for over one year when the callus was sucultured at 4-week interval. Upon transfer onto MS basal medium the callus gave rise to numerous somatic embryos and subsequently converted to plantlets. Plantlets were transplanted to potting soil and grown to maturity in the phyotron.

• Journal of Plant Biotechnology
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• v.32 no.3
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• pp.201-207
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• 2005

This study was conducted to examine the effect of BA and NAA on adventitious bud induction from in vitro germinants E. pellita. The capacity of adventitious bud formation greatly depends on juvenility and explants origin; the more juvenile materials are the better ability to form adventitious buds even in in vitro raised plantlets. In case of in vitro germinants, 7 day old plantlets showed a better morphological response than did 14 day old ones in the induction of adventitious buds. The capacity to show morphological response was in decreasing order : cotyledons> petioles> roots. Ho adventitious buds formed when root segments were used as culture material. And optimum medium appeared to be MS + 0.5 mg/L BA and 0.2 mg/L NAA. Adventitious buds could be developed into multiple shoots and regenerated normal plantlets on DKW medium plus 0.2 mg/L BA and 0.01 mg/L NAA.

• Journal of Plant Biotechnology
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• v.46 no.1
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• pp.9-16
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• 2019

Dendrobium candidum Wallich ex Lindley is a traditional Chinese medicine plant and has been widely used for medicinal and ornamental purposes. In this study, several different factors affecting micro propagation of protocorm-like bodies (PLBs) such as basal media, plant growth regulators, and carbon sources. The proliferation PLB derived from seeds was the best in $H_3P_4$ basal medium containing $0.1mg{\cdot}L^{-1}$ NAA and $0.1mg{\cdot}L^{-1}$ Kinetin. PLB growth was the best when $10g{\cdot}L^{-1}$ sucrose was added to the carbon atoms in the medium. The rate of shoot formation from the propagated PLB was the highest in 1/4 MS or $H_1P_2$ medium containing $10g{\cdot}L^{-1}$ sucrose, and the shoot length was longer than the others.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.50 no.6
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• pp.406-410
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• 2005

This study was conducted to establish the efficient protocols of the germination and cryopreservation of dehisced Korean ginseng seeds for long-term germ­plasm conservation. $GA_3$ and BA treated on seed for 24 hr facilitated germination at 5 and $10^{\circ}C$. Germination percentage of desiccated seeds was decreased under moisture content (MC) of below $7.2\%$. Dehisced ginseng seeds were dried under airflow of laminar floor cabinet and seed drying room. The high levels (more than $90\%$) of germination after cryogenic exposure were obtained after drying under vertical airflow of laminar floor for 12-30 hours (MC $10.6{\~}7.2\%$). Decrease in germination percentage of ginseng seeds due to desiccation damage and freezing injury was observed at MC of below $7.2\%$ and of above $12.1\%$, respectively. Therefore, MC of ginseng seeds need to be controlled with a range of $8{\~}10\%$ to avoid damages from both desiccation and freezing.

• Korean Journal of Weed Science
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• v.4 no.1
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• pp.62-68
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• 1984

This experiment was conducted to find out the possibility for reducing herbicidal dose of the recommended herbicides in combining with the plant growth regulator "chlorflurenol" at the Yeongnam Crop Experiment Station, from 1981 to 1982. Chlorflurenol itself exhibited weed suppression effect against Monochoria raginalis Presl., Rotala indica Koehne, Cyperus difformis L., Lindernia procrembens Philcox, etc. and its effect increased as the concentrations of chlorflurenol increased from 100 to 1,000 ㎖/㏊. Combination of chlorflurenol with butachlor or thiobencarb, showing synergistic effect, reduced their recommended herbicidal rates by 50 to 75%, showing 90% of weed control rate, stic but combined treatment between chlorflurenol and bentazon showed an antagonistic effect since bentazon had poor control ability to Echinochloa crusgalli Beauv. An application of chlorflurenol showed no significant effect on rice growth, in terms of dry weight, but it resulted in the decrease in the ratio of leaf blade to other non-photosynthetic organ.