• Journal of Bio-Environment Control
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• v.19 no.4
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• pp.397-402
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• 2010

This study was conducted to find out the appropriate type and concentration of plant growth regulator for mass production of wild type Hydrangea serrata. And the optimal soaking time for rooting of the cuttings was also investigated. When the cuttings of Hydrangea serrata were cultivated for 6 weeks after immersing in $50mg{\cdot}L^{-1}$ of IBA for 3 hrs, the number of roots was 90.6. However the number was significantly decreased when the cuttings were treated with $10mg{\cdot}L^{-1}$ of IBA. The rooting ratio was 100% when the cuttings was immersed in 1,000, 2000 and $3000mg{\cdot}L^{-1}$ of NAA solution for 5, 3 and 10 sec., respectively. Despite the perfect rooting, the number of roots was decreased. The number of roots was 105.5 when the cuttings were treated in $2,000\;mg{\cdot}L^{-1}$ of NAA for 5 sec., which was the highest number in NAA treatment. Overall, the rooting ratio and growth was better when the cutting was treated with low concentration of IBA ($50{\sim}250\;mg{\cdot}L^{-1}$) than the treatment of NAA. And the most appropriate concentration and time fot the treatment of IBA was $50mg{\cdot}L^{-1}$ and 3 hrs, respectively.

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 2001.04b
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• pp.81-85
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• 2001

작물은 빛으로부터 에너지를 공급받아 동화산물을 합성하고 이로부터 각종 대사작용에 필요한 물질을 생산한다. 따라서 빛은 작물이 생육하는데 있어 중요한 환경요인이지만 작물생육에 영향을 미치는 광량, 광질 및 일장 등은 장소, 시간 또는 시설물의 피복재료에 따라 변하기 때문에 이러한 요인들을 고려한 자연광의 효율적 이용은 시설재배 작물의 생장과 발육에 중요한 의미를 갖는다. (중략)

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.04a
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• pp.151-151
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• 2005

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.04a
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• pp.152-152
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• 2005

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.11a
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• pp.294-294
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• 2005

• Korean Journal of Plant Resources
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• v.10 no.1
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• pp.94-99
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• 1997

When the leaf and stem tissues of Rehmannia glutinosa were cultured on MS medium with plant growth regulators, shoots were regenerated on MS medium with TDZ(0.01 to $2.0{\mu}M$), and root initiation was better on MS medium treated with NAA(0.01 to 2.0mg/$\iota$). On $B_5$ medium, shoot regeneration was better on medium with TDZ than those with Quincrac, NAA, and 2.4-D. The addition of Quincrac, NAA, and 2.4-D inhibited shoot regeneration on MS medium, but promoted shoot regeneration on $B_5$ medium. Shoot regeneration and growth was better on MS medium with the combination treatments of 2.4-D 0.01mg/$\iota$ and TDZ 0.01, 0.1 and $2{\mu}M$ compared with other treatments. Also shoot regeneration and growth on B_5 medium showed the similar results to that on MS medium.

• Journal of The Korean Society of Grassland and Forage Science
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• v.21 no.1
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• pp.15-20
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• 2001

Plant growth regulators were treated on the cut perennial ryegrass (Lolium perenne L. cv. Reveille) to investigate the effect on the regrowth after cutting. The growth showed better result when 0.1 or 0.5 mg/L cytokinin were treated. Among cytokinins, kinetin or 2iP gave the better effect on the growth than BAP. In 2.4-D as an auxin. cut plants grew best at the concentration of 0.1 mg/L. The initial regrowth was very vigorous when GA$_3$ was treated as a growth regulator, but the growth was retarded after 2 weeks later of cutting. Co-treatment of kinetin as a cytokinin and 2,4-D as an auxin showed synergistic effect on the regrowth of cut perennial ryegrass. Both plant growth regulators gave the same result at the same concentrations in the suspension culture of perennial ryegrass cells.

• Korean Journal of Plant Tissue Culture
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• v.21 no.2
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• pp.69-76
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• 1994

Callus growth and shoot regeneration of Solanum and Lycopersicon depended on genotype, growth regulators, and thiamine concentration. L. peruvianum LA 1277 and L. peruvianum LA 1373 and PI 251301 had the greatest callus growth while L hirsutum LA 1777, L.esculentum 'Diego'and 'Red Plum' had the least callus growth. Lycopersicon penvianum genotypes were superior to L. esculentur genotypes in regenerating plants. MG medium was more effective in regenerating shoots than MS medium. A low level of IAA (0.2mg/L) and high level of BA (2 mg/L) resulted in the greatest shoot regeneration. Shoot growth varied depending on thiamine concentration and genotype. Shoot proliferation of Solanum ptycathum, Solanum nigrum, and L. peruvianum PI 199380 was best on medium with 20 mg/L thiamine. Regeneration of L. peruvianum PI 251301 and PI 128652 was better on medium with 30 and 10mg/L thiamine, respectively.

• KSBB Journal
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• v.17 no.6
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• pp.525-530
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• 2002

The optimum culture conditions for tropane alkaloid production in hairy root cultures of Korea native Scopolia paviflora Nak. were investigated. Hairy root was induced from the rhizome of the mother plant on B5 medium containing 1.0 mg/L IBA. Among the culture media examined, 1/2 B5 medium was the best for tropane alkaloid production, whereas the growth of hairy root increased in SH medium. The best result on the growth of hairy root was obtained in 1.0 mg/L NAA, and tropane alkaloid production was obtained in plant growth regulator-free medium. Of the carbone sources tested, 3% sucrose promoted the growth of hairy root, whereas 5% sucrose increased tropane alkaloid production. Optimum inoculum densities for root growth and tropane alkaloid production were 0.5 g and 1 g, respectively. The addition of XAD resins (1 % w/v) to hairy root cultures led to increases in tropans alkaloid production, and the release of alkaloid into the medium and its adsorption by the resin accounted for about 50 to 80％ of total production. It is concluded that optimized culture conditions and the addition of XAD resins could be used in the development of a bioprocess for tropane alkaloid production in hairy root cultures of S. paviflora Nak.

• Korean Journal of Plant Tissue Culture
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• v.28 no.1
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• pp.25-32
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• 2001

Shoot tips of grape were cultured in uitro and tried to identify optimal culture conditions for regeneration, multiple shoot formation from meristemoid tissue and those subsequent elongation of multi-shoots. Healthy growing shoots were taken in early May, rinsed with running tap water, soaked in a neutral detergent and washed with soft brushing, and washed out with tap water, then sterilized with 10g Ca(ClO)$_2$/140 mL distilled water (Wilson's solution) for 5 min. Survival percentage of the cultures which were sterilized as above procedures was highly increased, compared with the other sterilized method. Propagation of multi-shoots from meristemoid showed a good response in 3/4 strength MS medium enriched with 0.1 mg/L NAA and 3.0 mg/L BA. Shoot elongation from multi-shooting clump well occurred in 3/4 strength MS medium supplemented with 80 mg/L adenine sulfate, 0.1 mg/L NAA and 1.0~2.0 mg/L BA.