• Title/Summary/Keyword: 생육저해

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Effects of Blinds and Concentration of Salts on the Growth of Yeasts Isolated from Kimchi (소금 종류 및 농도에 따른 김치 효모균의 생육특성)

  • 한영숙;권민경;현영희;송주은;오지영
    • Journal of the East Asian Society of Dietary Life
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    • v.11 no.5
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    • pp.393-399
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    • 2001
  • Effects of various types of salts (commercial low salt, sea salt, refined salt, and bamboo salt) and concentrations (0, 3.0 and 5.0% ) on the growth of yeasts isolated from Kimchi were investigated. The isolated yeasts used in the study are as follows : Saccharomyces cerevisia, Sporobolomyces albo-rubescens, Issatchenkia orientalis Cryptococcus luteolous, Ustilago maydis, Candida humilis, Pichia onychis, Cadida nitratophila, and Pichia jadinii. The growths of the yeasts were inhibited against each salt concentration. The growths of Candida sp. isolated from the later stage of fermentation was strongly inhibited against 5% concentration of salt. On the other hand the induction phase of Issatchenkia orientalis isolated at the early stage of fermentation was the quickest among all the tested yeasts. Among the salts, bamoo salt was found to be strongest inhibitor of the growth of yeasts.

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Studies on the fluorescent Pseudomoηas isolated from the wheat rhizosphere (소맥근권(小麥根圈)에서 분리(分離)한 형광성(螢光性) Pseudomonas spp. 에 관(關)한 연구(硏究))

  • Lee, Myung-Chol;Kim, Yong-Woong;Kim, Kwang-Sik
    • Korean Journal of Soil Science and Fertilizer
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    • v.23 no.2
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    • pp.152-159
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    • 1990
  • Four active strains, producing siderophore and antagonizing to soil plant disease fungi, were isolated and identified from the wheat rhizosphere to reduce the injurious effects of continuous cropping and study on the biological control. The obtained results were summerized as follow. 1. Four strains of fluorescent Pseudomonas were isolated from the wheat rhizosphere and identified as Pseudomonas fluorescens Biotype A(Ps-1,2,5) and Biotype B(Ps-3) That strains inhibitied growth of R. solani, F.oxysperum and F. solani in vitro 2. Optical density of pigment was maximum at 410nm. 3. Siderophore production by identified strains was decreased with addition of $Fe^{+3}$, although not decreased with addition of $Fe^{+2}$ 4. Pigment of Ps-1, 2 and 3 strain inhibitied growth of R. slani, F. oxysperum and F. solani but pigment of Ps-5 strain did not inhibitie growth of R. solani 5. Effect of inoculation was in order of Ps-2, 1, 5 and 3 strain through the dark culture method, and effect of Ps-1 and Ps-2 was greater than that of treatment of captan 50ppm.

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Antimicrobial Activity of Bamboo (Sasa borealis) Leaves Fraction Extracts against Food Poisoning Bacteria (조릿대 잎 분획 추출물의 식중독균에 대한 항균활성)

  • Park, Yeon-Ok;Lim, Hyeon-Sook
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.12
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    • pp.1745-1752
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    • 2010
  • This study was conducted to investigate the antimicrobial activity of 70% ethanol (EtOH) extract and the five fractions of the crude extract from Sasa borealis leaves against seven food poisoning bacteria, Staphylococcus aureus, Micrococus luteus, Listeria monocytogens, Bacillus subtilis, Escherichia coli, Salmonella Typhimurium, and Pseudomonas aeruginosa. The yield of 70% EtOH extract was 11.4% and those of n-hexane, chloroform, ethyl acetate, n-butanol, and aqueous fractions were 3.0%, 1.1%, 0.6%, 1.3%, and 5.1%, respectively. The 70% EtOH extract and the four fractions except aqueous fraction demonstrated antimicrobial activity against all the seven food poisoning bacteria at a concentration of 0.5%, although it was less compared to benzoic acid. Minimal inhibitory concentration (MIC) of the 70% EtOH extract against all the food poisoning bacteria except S. aureus was $50{\mu}L$/disc. Moreover, chloroform fraction was $35{\mu}L$/disc against 3 food poisoning bacteria and $50{\mu}L$/disc against the other 4 food poisoning bacteria; ethyl acetate fraction was $50{\mu}L$/disc against all the food poisoning bacteria. In addition, n-butanol fraction was $50{\mu}L$/disc against all the food poisoning bacteria except S. aureus. Aqueous fraction, which did not show antimicrobial activity at 5%, was $200{\mu}L$/disc against only S. aureus and L. monocytogen. The 0.25%, and 0.5% of ethyl acetate fraction inhibited the growth of all the food poisoning bacteria 8 to 12 hours and 24 hours, respectively. These results indicate that the Sasa borealis leaves may be useful as a natural antimicrobial substance.

Effect of Water Treatment Sludge Application on the Growth of Korean Local Corn(Zeas Mays L.) (옥수수에 대한 정수 슬러지(Alum sludge)의 시용효과와 Aluminum이 옥수수의 유식물 생장에 미치는 영향)

  • Chang, Ki Woon;Koo, Ja Kong;Lim, Jae Shin;Kim, Young Han
    • Journal of the Korea Organic Resources Recycling Association
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    • v.3 no.1
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    • pp.73-83
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    • 1995
  • Effect of water treatment sludge(alum sludge) application on the growth of Korea local corn(Zeas Mays L.) was evaluated and phytotoxicity of alum sludge on the growth of corn roots and plant height was tested. Alum sludge itself and composted alum sludge compost were applicated by 1500kg/ha in the plot respectively. For the fertility test, yield, chlorophyll content, plant height of corn treated by alum sludge and composted alum sludge were better than that of control. No clear toxixity of alum sludge was found. At the food chemical analyses of seed corn, no obvious component variation were evaluated either. Test of pH effect on phytotoxicity of alum sludge on corns showed that plant height and root growth of corns were inhibited at low pH 4 solution. Phytotoxicity test of alum sludge extract which was extracted at various pH solutions, showed that growth and cations uptake inhibition were found the more serious the lower the pH. Plant height and root growth injury were serious at higher than 100ppm Al content solution.

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Inhibitory Effect of Shiitake Mushroom Extracts Cultured in Aloe-Supplement on Invasion/Migration of TNF-α-Induced MDA-MB-231 Breast Cancer Cells (알로에 배지에서 재배한 표고버섯 추출물의 종양괴사인자로 유도된 유방암세포 MDA-MB231에 대한 전이 저해 활성)

  • Suh, Seok-Jong;Kim, Cheorl-Ho;Baek, Jin-Hong;Lee, Kyoung-Hae;Shin, Dong-Bum;Park, Sung-Soo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.44 no.1
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    • pp.44-48
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    • 2015
  • To investigate the anti-cancer effect of shiitake mushrooms (Lentinus edodes) cultured in aloe-supplement, we treated extract of shiitake mushroom cultured in aloe-supplement (ESA) to MDA-MB-231 human breast cancer cells. ESA-treated MDA-MB-231 cells showed decreased growth rate in XTT assay. In addition migration/invasion was noticeably inhibited by ESA in TNF-${\alpha}$-treated MDA-MB-231 cells. Western blot analysis showed that the molecular mechanism of cell migration/invasion was mediated by reduced intercellular adhesion molecule-1 expression via p-ERK signal transduction pathways. We found ESA had inhibition activity against cellular growth and migration/invasion. Taken together, ESA has putative anti-cancer activity against human breast cancer.

Changes in Growth Characteristics of Waxy Corn 'Ilmichal' due to Low Temperature during the Seedling Stage (일미찰옥수수의 유묘기 저온에 따른 생육특성 변화)

  • Jeon, Seung Ho;Oh, Seung Ka;Kim, Han Yong;Na, Chae-In;Bae, Hui Su;Cho, Young Son
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.65 no.4
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    • pp.426-435
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    • 2020
  • To determine the damages to waxy corn caused by low temperature weather, we investigated the relationship between the temperature and duration of low temperature treatment and the changes in growth characteristics during the recovery period after the treatments in different growth periods. Growth inhibition started in the low temperature group treated at temperatures below 5℃ for three days. The inhibition ratio (IR) was more than 22% or more in all sample groups. As the treatment duration increased and the Temperature decreased, the growth was more greatly inhibited than that in the control. The IR was the highest at 27% or more in the 2nd leaf stage group treated at below 5℃ for 5 days. The IR was in the order of 2nd leaf stage > 1st leaf stage > coleoptile. The IR during recovery was the highest in the 2nd leaf stage group treated at -3℃ degree for 7 days, nd the values were 82% and 98% for NDVI and Fv/Fm, respectively. Especially, all groups treated at -3℃ showed either no changes or decreases in the growth characteristics. As a result, growth inhibition increased as the temperature decreased, and as the duration of the low temperature increased. The degree of damage was in the order of 2nd leaf stage > 1st leaf stage > coleoptile. All early seedlings stopped growing and withered when exposed to temperatures at or below -3℃ for 3 days or more.

Effects of Tannins from Astringent Persimmons in Alcohol Fermentation for Persimmon Vinegars (떫은감 탄닌성분이 감식초의 알콜발효에 미치는 영향)

  • 서지형;정용진;신승렬;김광수
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.29 no.3
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    • pp.407-411
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    • 2000
  • 감식초를 제조할 때 떫은감에 함유된 탄닌성분이 알콜발효균주의 생육 및 알콜발효물의 품질에 미치는 영향에 대해 조사하였다. 알콜발효균주에 대한 생육저해는 녹숙감에서 분리된 탄닌에서 가장 뚜렷했으며 그 뒤로 완숙기, 연시 탄닌 순으로 나타났고, S cerevisiae YJK20에 비해 S, kluyveri DJ97이 생육저해를 적게 받았다. 알콜발효액의 단도는 첨가한 탄닌함량이 높을수록 서서히 감소하여 0.2%, 0.3%탄닌처리고(III, IV)는 발효 3일까지 그 감소가 현저하였으나, 발효6일에는 모든 처리구에서 5.8~6.0$^{\circ}$Brix로 일정수준을 보였다. 발효액의 알콜함량은 참가한 탄닌함량이 낮을수록 단기간에 높은 알콜함량을 나타내었으나, 각 처리구의 최고 알콜함량에서는 큰 차이가 없었다. 또한 첨가한 탄닌함량이 높을수록 발효액의 a값 증가가 뚜렷하여 최종 발효액에서 갈색도를 나타내는 a/b값이 높은 것으로 나타났다. 탁도도 발효 1일에 각처리구에서 거의 동일한 수준이었으나, 알콜\ulcorner량이 최고치를 나타내는 발효 4일에는 0.3% 탄닌처리구(IV)에서 가장 높았으며, 각 처리구의 총페놀성 물질 및 가용성 탄닌의 함량은 발효가 진행됨에 따라 감사하였다.

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Analysis of the Bacterial Community in Ojingeo-jeotgal and Selection of Bacillus Species Inhibiting the Growth of Food Pathogens (오징어젓갈 Bacteria 군집분석 및 식중독균 생육저해 Bacillus 균주 선발)

  • Kim, Hye-Rim;Han, Seulhwa;Lee, Bitnara;Jeong, Do-Won;Lee, Jong-Hoon
    • Microbiology and Biotechnology Letters
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    • v.41 no.4
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    • pp.462-468
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    • 2013
  • Jeotgal is a generic term given to the high-salt-fermented seafood of Korea. This study aimed at developing an overview of the bacterial community present in Ojingeo-jeotgal, a highly consumed type of jeotgal, which is made with squid. Bacteria were isolated and purified from two samples on six different kinds of media and identified by 16S rRNA gene sequence analysis. Among the 121 total isolates, the most dominant genus was Bacillus, followed by coagulase-negative staphylococci (CNS) and lactic acid bacteria (LAB). CNS were detected in both samples, but LAB were observed in only a single sample. Six strains of Bacillus species inhibiting the growth of food pathogens, Staphylococcus aureus and Vibrio parahaemolyticus, were selected from the 121 isolates. These were found to inhibit the growth of both pathogens in addition to displaying proteolytic activities on media containing 6% NaCl and 2% skim milk.

Effects of Ethanol Extracts from Chrysanthemum Petals on the Growth Inhibition of Microorganisms (미생물의 생육억제에 대한 국화 에탄올 추출물의 영향)

  • 정용진;이명희;이기동;박난영;권중호
    • Food Science and Preservation
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    • v.5 no.3
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    • pp.299-304
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    • 1998
  • Screening for antibacterial activities with microorganisms related to the food putrefaction by ethanol extract from Chrysanthemum petals widely used for the traditional wine production, and antibacterial activities of each concentration and minimum inhibitory concentration(MIC) from the ethanol extract were researched. Antibacterial activity of ehanol extract for B. subtilis was higher in C. boreale than in C. Morifolium, but that of E. coli was higher in C. molifolium than in C. boreale. C. boreale was higher than C. morifolium in the antibacterial activity of ehanol extract and MIC of ehanol extract from C. boreale was 60-70${\mu}\ell$/ml. Ethanol extract from C. boreale was higher Gram(-) than Gram(+) in the antibacterial activities, but Gram(-), Gram(+) were greatly inhibited on growth in 100${\mu}\ell$ concentration.

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Antimicrobial Activities of Extracts of Prunus mume by Sugar (매실 당침출액의 항균활성)

  • Ko, Myung-Soo;Yang, Jong-Beom
    • Food Science and Preservation
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    • v.16 no.5
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    • pp.759-764
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    • 2009
  • The antimicrobial activities of extracts of Prunus mume by sugar against food poisoning bacteria, and the effects of heat and pH treatment on these antimicrobial activities, were investigated. The level of total solids, pH, and acidity of P. mume extracts were 55.08% (w/w), pH 3.1, and 1.52%, respectively. P. mume extracts showed the strongest antimicrobial activity against Vibrio parahaemolyticus, among the bacteria tested. P. mume extracts significantly inhibited the growth of V. parahaemolyticus, Bacillus cereus, and Staphylococcus aureus at levels of 1-2% (w/v) of extracts in media. The antimicrobial activities of P. mume extracts were neither affected by heating at $65-125^{\circ}C$ for 30 minutes, nor by neutralization of extract to pH 7.0.