Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Inhibitory Effect of Shiitake Mushroom Extracts Cultured in Aloe-Supplement on Invasion/Migration of TNF-α-Induced MDA-MB-231 Breast Cancer Cells

알로에 배지에서 재배한 표고버섯 추출물의 종양괴사인자로 유도된 유방암세포 MDA-MB231에 대한 전이 저해 활성

  • Suh, Seok-Jong (Department of Biological Science, Sungkyunkwan University) ;
  • Kim, Cheorl-Ho (Department of Biological Science, Sungkyunkwan University) ;
  • Baek, Jin-Hong (R&D Center, KJM Aloe) ;
  • Lee, Kyoung-Hae (Department of Food Science & Biotechnology, Dongnam Health College) ;
  • Shin, Dong-Bum (Department of Food Science and Nutrition, Jeju National University) ;
  • Park, Sung-Soo (Department of Food Science and Nutrition, Jeju National University)
  • 서석종 (성균관대학교 생명과학과) ;
  • 김철호 (성균관대학교 생명과학과) ;
  • 백진홍 ((주)김정문알로에 생명과학연구소) ;
  • 이경혜 (동남보건대학교 식품생명과학과) ;
  • 신동범 (제주대학교 식품영양학과) ;
  • 박성수 (제주대학교 식품영양학과)
  • Received : 2014.11.17
  • Accepted : 2014.12.22
  • Published : 2015.01.31
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Abstract

To investigate the anti-cancer effect of shiitake mushrooms (Lentinus edodes) cultured in aloe-supplement, we treated extract of shiitake mushroom cultured in aloe-supplement (ESA) to MDA-MB-231 human breast cancer cells. ESA-treated MDA-MB-231 cells showed decreased growth rate in XTT assay. In addition migration/invasion was noticeably inhibited by ESA in TNF-${\alpha}$-treated MDA-MB-231 cells. Western blot analysis showed that the molecular mechanism of cell migration/invasion was mediated by reduced intercellular adhesion molecule-1 expression via p-ERK signal transduction pathways. We found ESA had inhibition activity against cellular growth and migration/invasion. Taken together, ESA has putative anti-cancer activity against human breast cancer.

알로에를 첨가한 표고버섯 배양 추출물(ESA)의 항암효과를 검정하기 위하여 인간 유방암 세포주인 MDA-MB-231에 ESA를 처리하여 생육저해 활성과 세포 이동 억제 효과를 확인하였다. MDA-MB-231 세포에 ESA를 처리하여 72시간 후 관찰한 결과 약간의 생육저해 활성을 나타내었으며, 또한 transwell에서 TNF-${\alpha}$가 처리된 세포에 ESA를 처리해 본 결과 migration/invasion 정도를 상당히 저해함을 알 수 있었다. 이러한 세포 이동능의 저해 기작은 웨스턴 블럿 분석을 통해 확인해 본 결과 p-ERK 신호를 통해 일어나며, ICAM-1 단백질의 발현을 억제함으로써 나타나는 것을 알 수 있었다. 따라서 본 실험에서는 알로에 첨가 담자균 배양추출물(ESA)은 인간 유방암세포에 대해 잠재적인 항암효과가 있음을 확인하였다. 이후 표고버섯 외 꽃송이버섯과 상황버섯에서의 알로에 배양액에 대한 항암효과에 대한 연구를 수행하고 있어 그 결과를 보고하고자 한다.

Keywords

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