• Journal of Bio-Environment Control
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• v.30 no.3
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• pp.218-229
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• 2021

Recently, as consumers' interest and importance in health care have significantly increased, they prefer natural and organic foods that do not use chemical pesticides. Since sprout vegetables effectively promote health and prevent diseases such as cancer and cardiovascular disease, the consumption of sprout vegetables, a highly functional and safe food, has been increased significantly. This study aimed to investigate the effect of light on the growth and bioactive compounds of seven different sprout vegetables. After sowing the seeds of various sprout vegetables (kale, Chinese kale, broccoli, red cabbage, alfalfa, red radish, and radish), the sprouts were cultivated under light conditions (20℃, RGB 6:1:3, 130 μmol·m^-2·s^-1, 12 hours photoperiod) and dark condition for 7 days. Sprouts samples were taken at 1-day intervals from 4 to 7 days after treatment. The fresh weight, dry weight, plant height, total phenol content, and antioxidant capacity were measured. Brassica species (kale, Chinese kale, broccoli, red cabbage) and Medicago species (alfalfa) had significantly higher fresh weight values under dark conditions, while the content of bioactive compounds was increased considerably under light conditions. In contrast, the fresh weight of Raphanus genus (red radish, radish) significantly increased under the light condition, but the antioxidant phenolic compounds were significantly higher under the dark state. A negative correlation was observed between the growth and secondary metabolites in various sprout vegetables. This study confirmed the effect of light and dark conditions on different sprout vegetables' growth and nutritional value and emphasizes the importance of harvest time in producing high-quality sprout vegetables.

• Journal of Periodontal and Implant Science
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• v.33 no.1
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• pp.27-35
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• 2003

1. 목적 생체재료의 생체친화성을 증진시키고 치유를 촉진하기 위한 목적으로 생체재료의 생화학적 표면개질에 관한 연구가 널리 진행되고 있다. 이와 같은 목적으로 이용되어 온 부착분자에는 아미노산, 펩타이드, 단백질, 효소 및 성장인자들을 들 수 있으며, 이들 분자들을 금속, 골대체물질 및 폴리머와 같은 생체재료의 표면개질에 이용하여 왔다. 이 연구의 목적은 생체적합성이 우수하고 생분해성을 지닌 키토산으로 얇은 막을 제작한 후, 세포외 기질의 구성성분 중 세포부착에 관여하는 RGD 펩타이드를 부착시킨, 표면개질 키토산막의 생물학적 영향을 MG-63 조골양세포를 이용하여 관찰하는 것이다. 2. 방법 2% acetic acid에 키토산 가루를 녹여 만든 2% 키토산 용액으로 24-well 배양접시의 표면을 도포 후 24시간 동안 건조시켜 키토산막을 제작하였다. GRGDS 펩타이드를 cross-linker(EDC, NHS) (Sigma, MO, USA) 용액과 반응시켜서 펩타이드의 카르복실기를 활성화시켰다. 이들을 PBS 완충용액으로 수화시킨 키토산막과 결합시켜 펩타이드의 활성화된 카르복실기와 키토산의 아민기 간에 안정적인 아미드 결합(amide bond)이 형성되도록 하였다. 하루 동안 반응을 일으킨 후 PBS 완충용액과 증류수로 씻어내고 냉동 건조시킴으로써 GRGDS가 결합된 키토산막을 제작하였다. 재료 표면의 화학 성분을 알아보는데 사용되는 방법의 일종인 X-ray photoelectron spectroscopy(XPS) 분석을 통하여 부착분자가 키토산막에 결합된 여부를 확인하였다. GRGDS 펩타이드에 요오드를 결합시킨 후, 이것을 키토산막에 공유 결합시키고 XPS를 통해 요오드가 재료 표면에서 검출되는지를 검사하였다. 요오드가 검출된다면 이것은 키토산막 표면에 실제로 GRGDS 펩타이드가 존재하는 것을 의미하게 된다. 표면개질된 키토산막에 사람조골양세포인 MG-63을 접종하여 이를 실험군으로 하였고, 표면이 개질 되지 않은 키토산막을 대조군으로 하였다. 세포부착의 최적화 농도를 확인하기 위하여 GRGDS를 0.01, 0.05, 0.1, 0.25, 0.5, 1.0mg/ml의 농도로 준비하였다. 배양 후 1일, 7일째에 각 well에서 trypsin EDTA를 이용하여 세포를 분리한 후, 이를 원심 분리하여 세포수측정기를 이용하여 부착 세포의 수를 측정하여 세포의 부착 정도를 비교하였다. 배양 2시간, 24시간 후 주사전자현미경을 이용하여 키토산막에 부착된 세포의 양상을 관찰하였다. 3. 결과 XPS를 통한 표면의 화학 성분 분석 결과 GRGDS 펩타이드를 결합시킨 키토산막에서 요오드가 검출되었으며 펩타이드를 부착하지 않은 대조군에서는 검출되지 않았다. 따라서 cross-linker를 이용한 펩타이드와 키토산막의 공유결합을 확인할 수 있었다. 세포 배양 후 1일째 부착된 세포 수를 측정한 결과 0.1mg/ml 이상의 GRGDS 펩타이드 농도로 공유 결합시킨 키토산막에서 부착 세포 수가 다른 농도에 비해 유의성 있게 많이 관찰되었다. 이 농도 이하에서는 대조군과 실험군간에 세포부착의 유의한 차이가 없었다. 따라서 주사전자현미경을 이용한 부착 세포의 양상에 관한 관찰은 0.1mg/ml 농도의 펩타이드를 이용하였다. 세포 배양 7일째, 부착된 세포 수 측정 결과 GRGDS의 농도에 따른 유의성 있는 차이가 없었으며, 실험군과 대조군간에도 유의성 있는 차이가 없었다. 주사전자현미경 관찰결과 2시간 및 24시간 배양된 실험군 모두에서 별모양의 세포들이 키토산막 표면에 편평하게 잘 부착되어 있으며 많은 위족이 발달된 소견을 보인 반면, 대조군에서는 원형 또는 다각형 모양의 세포들이 실험군에 비해 부착이 덜 되어있는 양상을 보였다. 이 연구를 통하여 기능성 펩타이드를 생체재료의 표면에 공유결합 시키는 방법을 확립할 수 있었으며, RGD 펩타이드의 공유결합으로 표면개질된 키토산막이 조골세포의 부착능을 증진시킬 수 있음을 확인하였다. 표면개질된 생체재료를 소, 중동물에 적용시켜 생체 내에서의 생물학적 영향을 평가할 필요가 있으며, 이 실험의 결과는 향후 다양한 기능성 부착분자를 선발, 고안하여 임플란트용 생체재료의 표면개질에 이용하는 이른바 모방생체재료분야에 널리 활용될 수 있을 것으로 생각된다.

• Korean Journal of Agricultural and Forest Meteorology
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• v.17 no.3
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• pp.191-201
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• 2015

Measuring and mapping connectivity among habitats is a key component of sustainable urban planning and design process. In this study, we examined how functional corridors connect forest bird habitats in a metropolitan area of Korea using graph theory-based techniques. High-quality forest habitat was defined as a function of forest cover, presence of residential areas, and road networks. We then constructed a network of high-quality forest habitats using the FunConn (functional connectivity) tools, and computed metrics ($T_i$) of patch importance based on the minimum ($Q_1$) and the 25th percentile ($Q_{25}$) rank least-cost distance values. We investigated the relative influence of two values of patch importance on forest bird species richness. As a result, the patch importance index based on the $Q_{25}$ effective distance threshold was most positively correlated with species richness (P < 0.001) after controlling for the area effect. Thus, using the $Q_{25}$ effective distance threshold, we mapped not only the locations of important habitat patches and functional corridors, but also the network backbone of forest bird habitats. The network developed in this study can help guide urban planning for biodiversity conservation.

• Journal of Environmental Policy
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• v.13 no.3
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• pp.3-19
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• 2014

Large-scale unband development resulted from the rapid economic growth in the Republic of Korea has brought about the habitat destruction for the native animals and plants living in forest and farmland. In order to resolve this problem, it is necessary to consider the natural ecosystem as an organism and to preserve the natural ecosystem by managing ecologically significant habitat consistently. Especially, the local governments should be able to establish regional ecologic networks in consideration of the ecological connectivity and the environmental and ecological excellence, and to reflect them into the local development plans. In regard to this, the methods of the regional ecological network establishment was presented, making use of the results including the national ecological network which assessed the ecological connectivity of the nation and the environmental and ecological assessment results of the Environmental Conservation Value Assessment Map which was designed to analyze and assess the national environmental and ecological values quantitatively. Making use of the case of Gyeongsangnam-do; the results presented that the existing national ecological network in the core region has expanded from $2,986km^2$ to $4,049km^2$ and the existing national ecological network in the buffer region has expanded from $2,940km^2$ to $3,006km^2$. Referring to the regional ecological network in the process of the local development plans could contribute to the increase in biodiversity and the integrated local environmental management including the ecosystem preservation.

• Korean Journal of Environment and Ecology
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• v.33 no.2
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• pp.237-251
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• 2019

This study evaluated the ecological value of waterside parks by investigating the animal distribution and ecological feature in 92 waterside parks and analyzed the change of ecological awareness by users and non-users of waterside parks through ecological education and promotion based on the investigation results. The result confirmed inhabitation of various animals including 9 endangered species (Pernis ptilorhynchus orientalis, Accipiter soloensis, Falco subbuteo, Charadrius placidus, Felis bengalensis euptilura, Lutra lutra, Kaloula borealis, Polyphylla laticollis manchurica, and Leptalina unicolor) in waterside parks. Although waterside parks were constructed to be hydrophilic areas for human use, some of them with high natural characteristics are valued as biological habitat. We investigated user status in 5 areas (Daejeon, Sejong, Cheongju, Kongju, and Buyeo) located at Guem river basin to evaluate people's perception of waterside parks and carried out the ecological education and promotion based on the investigation result. The survey of 200 people showed that there were more users of waterside parks than non-users and that people in their 40's showed the highest use rate. The use frequency of waterside parks located in Daejeon and Cheongju was lower than in other areas (Sejong, Kongju, and Buyeo). We considered it was because Daejeon and Cheongju were urban areas and had relatively more leisure areas such as sports facilities and cafe than other areas, and thus the residents had a lower reliance on waterside parks. Moreover, users used waterside parks more frequently when they were nearer to users' residence. It is because most users perceived waterside parks as the leisure sports facility and thus preferred them to be within walking distance. The users' perception of waterside parks as the ecological space "to be preserved" increased after the ecological education and promotion. The change of the perception was higher among users (80%) than non-users (38%). Therefore, ecological education and promotion were potentially more effective to people who user waterside parks and thus had a higher understanding of the characteristics and specification. In conclusion, 1) although waterside parks were constructed for human use, some parts had high ecological value for the distribution of endangered species and outstanding natural beauty, and 2) it is necessary to change the perception of waterside parks from the hydrophilic attribute to the conservation attribute. Such change of perception would contribute to establishing waterside parks that feature both hydrophilic and conservation attributes in the management or upgrading plan of waterside parks in the future.

• Korean Journal of Environment and Ecology
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• v.36 no.5
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• pp.507-524
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• 2022

In this study, we revealed the location environment and community structural characteristics after extensively investigating Korea's warm-temperate island areas and categorizing vegetation through TWINSPAN analysis. Based on it, this study aims to suggest the direction of the vegetation restoration plan for warm-temperate forests by deriving a restoration strategy for each vegetation type. The vegetation types were clearly divided into eight types, and communities I through IV were good evergreen broad-leaved forests dominated by Machilus thunbergii and Castanopsis sieboldii. On the other hand, communities V through VIII were Pinus thunbergii forest, deciduous broad-leaved forest, and artificial forest, and retrogressive succession vegetation in the warm-temperate areas. The environmental factors derived from the DCA analysis were altitude (average temperature of the coldest month) and distance from the coastline (salt tolerance). The distribution pattern of warm-temperate forests has been categorized into M. thunbergii, C. sieboldii and Cyclobalanopsis spp. forest types according to the two environmental factors. It is reasonable to apply the three vegetation types as restoration target vegetation considering the location environment of the restoration target site. In communities V through VIII, P. thunbergiiand deciduous broad-leaved formed a canopy layer, and evergreen broad-leaved species with strong seed expansion frequently appeared in the ground layer, raising the possibility of vegetation succession as evergreen broad-leaved forests. The devastated land where forests have disappeared in the island areas is narrow, but vegetation such as P. thunbergii and deciduous broad-leaved forests, which have become a retrogressive succession, forms a large area. The restoration strategy of renewing this area into evergreen, broad-leaved forests should be more effective in realizing carbon neutrality and promoting biodiversity.

• Journal of Environmental Impact Assessment
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• v.29 no.1
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• pp.26-36
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• 2020

This study aimed at investigating present research and knowledge-base on climate change adaptation in ecosystem sector and analyzed the current status of basic information on ecosystem that functions as evidence-base of climate change adaptation to deduce the suggestions for the future development for knowledge and information in biodiversity. In this perspective, a questionary survey titled as "the ecological knowledge-base and information needs for climate change adaptation" with the researchers who were engaged with adaptation studies for biodiversity in the ecosystem related-research institutes including national and 17 regional local governments-affiliated agencies in Korea. The results are as follows; current status of utilizing ecological information which supports climate change adaptation strategy, future needs for adaptation knowledge and ecological information, and activation of utilizing ecological information. The majority of respondents (90.7%) replied that the ecological information has high relevance when conducting research on climate change adaptation. However, only half of all respondents (53.2%) agreed with the real viability of current information to the adaptation research. Particularly, urgent priority for researchers was deduced as intensifying knowledge-base and constructing related information on 'ecosystem change from climate change (productivity, community structure, food chain, phenology, range distribution, and number of individuals) with the overall improvement of information contents and its quality. The respondents emphasized with the necessity of conducting field surveys of local ecosystem and constructing ecosystem inventories, advancing monitoring designs for climate change in ecosystem, and case studies for regional ecosystem changes with the guidance or guidelines for monitoring ecosystem change to enhance the quality of adaptation research and produce related information. In terms of activation for ecological information usage, national and local adaptation network should be working based on the integrated ecological platform necessary to support exchanges of knowledge and information and to expand ecosystem types in time and spatial dimension.

• Journal of Digital Convergence
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• v.18 no.12
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• pp.585-594
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• 2020

At present, aging-related degenerative muscle diseases are considered a serious problem. However, the drug effect on the treatment and prevention of sarcopenia has not been investigated. The purpose of this study was to evaluate the value of extract of Pu'er tea as a remedy to alleviate the symptoms of sarcopenia. Pu'er tea extracts showed excellent radical scavenging ability. The expression of Myh3 was promoted and myotube formation also was increased by treatment of Pu'er tea extracts. These results suggest that Pu'er tea is a natural substance that promotes myogenesis, and is valuable as a material for pharmaceutical research on the prevention and treatment of various muscle diseases, including sarcopenia. And It is necessary to confirm specific indicator substances of puer tea and further study on this.

• Journal of the Korean Institute of Landscape Architecture
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• v.52 no.1
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• pp.71-86
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• 2024

Despite the increasing importance of urban parks' ecological functions in dealing with the climate crisis, ecological parks are not clearly defined in Korea's legal system. Numerous ecological parks created nationwide cannot be systematically designated and managed due to various legal bases and varying management authorities. It is important to clarify the legal status of ecological parks in order to lead the ecological paradigm shift of urban parks and to improve the natural park system for a comprehensive and integrated approach to protect the national ecosystem. To this end, related laws were analyzed to identify problems and to draw directions for legislative improvement. Through the literature review of relevant laws, acts, and ordinances, six major directions for improvement were suggested based on the analysis of problems. First, the legal status of ecological parks in the administrative dichotomy of the current park system is ambiguous, and ecological parks should be clarified through the revision of park-related laws. Second, an ecological park can be defined as a sustainable park created and managed in an ecological manner, promoting the protection and restoration of the ecosystem, conservation, and promotion of biodiversity, and balancing nature observation, ecological learning, and leisure activities. Third, the role of the state and local governments should be systematically revised to lead to a new park planning and management model through new governance. Fourth, since the characteristics of ecological parks are affected by individual laws, the possibility of overlapping ecological parks for other uses should be allowed. Fifth, detailed guidelines and standard ordinances need to be enacted to meet the goals, principles, and facilities of ecological parks. Lastly, along with the revision of the laws, ordinances by local governments also need to be more concrete. This study, which tracks various legal realities related to ecological parks, can contribute to policymaking that can systematize the foundation for the creation of ecological parks to preserve nationwide ecosystems and provide citizens with opportunities to experience and learn about nature.

• Korean Journal of Environment and Ecology
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• v.30 no.5
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• pp.837-847
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• 2016

In this study, the bird fauna of Sihwa Lake in Gyeonggi Province were investigated from 2009 to 2014 to identify the effect of the restored intertidal zone on bird community. Based upon the analysis of the habitat status and distribution change of water birds in the intertidal zone, the following conclusions were obtained. The number of water bird individuals increased by approx. 40 thousand after the restoration. Most of water bird species increased, but diving ducks decreased. Analysis of the number of individuals of water birds before and after the intertidal zone restoration showed that there was a significant increase in the number of shorebird individuals. There is a habitat use pattern change in the southern tideland intertidal zone that includes the largest area of widened intertidal zone and the inland waters of the intertidal zone. This is related to the change of benthic ecosystem which is the food source of birds. The benthic ecosystem in restoring Sihwa Lake has been stabilized with a decrease in opportunistic species and consistent increase in equilibrium species that are sensitive to pollution. As a result, the environmental conditions for shorebirds have been improved. The restoration of intertidal zone affected the habitat use and distribution patterns of water birds in a short period of time. Compared to before restoration, all the water birds now use the wide area of the intertidal zone with no partiality and it is confirmed that the restoration greatly affected the distribution of shorebirds and swan & geese. This study was conduced to identify the effects of an artificial restoration of intertidal zone due to tidal power generation of bird species. In terms of maintaining and improving biodiversity, the intertidal zone restoration was recognized to be important and still shows positive results. This study is expected to provide a direction when an alternative is required to maintain and improve biodiversity in a similar situation in the future.