DOI QR코드

DOI QR Code

Growth and Bioactive Compound Contents of Various Sprouts Cultivated under Dark and Light Conditions

광 유무에 따른 다양한 새싹 채소의 생육 및 생리활성 화합물의 함량

  • Lee, Jin-Hui (Graduate School of Horticulture, Chiba University) ;
  • Oh, Myung-Min (Division of Animal, Horticultural, and Food Sciences, Chungbuk National University)
  • 이진희 (치바대학교 원예학과) ;
  • 오명민 (충북대학교 축산.원예.식품공학부 원예학전공)
  • Received : 2021.07.14
  • Accepted : 2021.07.27
  • Published : 2021.07.31

Abstract

Recently, as consumers' interest and importance in health care have significantly increased, they prefer natural and organic foods that do not use chemical pesticides. Since sprout vegetables effectively promote health and prevent diseases such as cancer and cardiovascular disease, the consumption of sprout vegetables, a highly functional and safe food, has been increased significantly. This study aimed to investigate the effect of light on the growth and bioactive compounds of seven different sprout vegetables. After sowing the seeds of various sprout vegetables (kale, Chinese kale, broccoli, red cabbage, alfalfa, red radish, and radish), the sprouts were cultivated under light conditions (20℃, RGB 6:1:3, 130 μmol·m-2·s-1, 12 hours photoperiod) and dark condition for 7 days. Sprouts samples were taken at 1-day intervals from 4 to 7 days after treatment. The fresh weight, dry weight, plant height, total phenol content, and antioxidant capacity were measured. Brassica species (kale, Chinese kale, broccoli, red cabbage) and Medicago species (alfalfa) had significantly higher fresh weight values under dark conditions, while the content of bioactive compounds was increased considerably under light conditions. In contrast, the fresh weight of Raphanus genus (red radish, radish) significantly increased under the light condition, but the antioxidant phenolic compounds were significantly higher under the dark state. A negative correlation was observed between the growth and secondary metabolites in various sprout vegetables. This study confirmed the effect of light and dark conditions on different sprout vegetables' growth and nutritional value and emphasizes the importance of harvest time in producing high-quality sprout vegetables.

최근, 소비자들이 건강관리에 대한 관심과 중요도가 높아짐에 따라 화학적인 농약을 사용하지 않은 유기농 천연 식품을 선호하게 되었다. 새싹 채소는 건강증진과 암, 심혈관질환 등의 질병을 예방하는 효과가 있어, 고기능성이고 안전한 식품인 새싹 채소의 소비가 크게 증가하고 있는 추세이다. 이 연구의 목적은 여러가지 새싹 채소 7종의 생육 그리고 생리활성 물질 함량에 대한 광의 영향을 조사하는 것이다. 새싹 채소용 케일, 다채, 브로콜리, 적양배추, 알팔파, 홍빛 열무 그리고 무 종자를 파종한 뒤 새싹들을 광조건(20℃, RGB 6:1:3, 130μmol·m-2·s-1, 12시간 광주기), 암조건 아래에서 각각 7일간 재배하였다. 처리 후 4일째부터7일째까지 1일 간격으로 샘플을 채취하였고 생체중과 건물중, 초장, 총페놀 함량, 항산화도를 측정하였다. Brassica 종(케일, 다채, 브로콜리, 적양배추)과 Medicago 종(알팔파)은 암조건에서 생체중 값이 유의적으로 높았지만 생리활성 물질 함량은 광조건에서 유의적으로 증대되었다. 이에 반해 Raphanus 속(홍빛 열무, 무)는 광조건에서 생체중이 유의적으로 증대되었지만 생리활성 물질 함량은 암조건에서 유의적으로 높은 값을 나타내었다. 다양한 새싹 채소의 생육과 생리활성 물질 함량 사이에서 음의 상관관계가 관찰되었다. 본 연구는 다양한 새싹 채소의 생육 및 영양학적 가치에 대한 광의 영향을 확인하였고 고품질의 새싹 채소를 생산하는데 있어 수확시기의 중요성을 강조한다.

Keywords

Acknowledgement

본 결과물은 농림축산식품부 및 과학기술정보통신부, 농촌진흥청의 재원으로 농림식품기술기획평가원과 재단법인 스마트팜연구개발사업단의 스마트팜다부처패키지혁신기술개발사업의 지원을 받아 연구되었음(421033-4).

References

  1. AbdElgader A., S. Aiemla-Or, C. Wongs-Aree, P. Jitareerat, and A. Uthairatanakij 2015, Effect of LED lighting on the quality of radish sprout. Agric Sci 46:888-891.
  2. Aguilera Y., M.F. Diaz, T. Jimenez, V. Benitez, T. Herrera, C. Cuadrado, M. Martin-Pedrosa, and M.A. Martin-Cabrejas 2013, Changes in nonnutritional factors and antioxidant activity during germination of nonconventional legumes. J Agric Food Chem 61:8120-8125. doi:10.1021/jf4022652
  3. Ainsworth E.A., and K.M. Gillespie 2007, Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin-Ciocalteu reagent. Nat Protoc 2:875-877. doi:10.1038/nprot.2007.102
  4. Ampofo J.O., and M. Ngadi 2021, Stimulation of the phenylpropanoid pathway and antioxidant capacities by biotic and abiotic elicitation strategies in common bean (Phaseolus vulgaris) sprouts. Proc Biochem 100:98-106. doi:10.1016/j.procbio.2020.09.027
  5. Baskin J.M., and C.C. Baskin 1989, Role of temperature in regulating timing of germination in soil seed reserves of Thlaspi arvense L. Weed Res 29:317-326. doi:10.1111/j.1365-3180.1989.tb01301.x
  6. Bian Z., R. Cheng, Y. Wang, Q. Yang, and C. Lu 2018, Effect of green light on nitrate reduction and edible quality of hydroponically grown lettuce (Lactuca sativa L.) under short-term continuous light from red and blue light-emitting diodes. Environ Exp Bot 2018, 153:63-71, doi:10.1016/j.envexpbot.2018.05.010
  7. Boo H.O., and B.Y. Lee 1999, Effect of light on the biosynthesis of anthocyanin in Brassica oleracea var. capitata f. rubra L. Kor J Soc Hort Sci 40:322-326.
  8. Caceres P.J., C. Martinez-Villaluenga, L. Amigo, and J. Frias 2014, Maximising the phytochemical content and antioxidant activity of Ecuadorian brown rice sprouts through optimal germination conditions. Food Chem 152:407-414. doi: 10.1016/j.foodchem.2013.11.156
  9. Castillejo N., L. Martinez-Zamora, P.A. Gomez, G. Pennisi, A. Crepaldi, J.A. Fernandez, and F. Artes-Hernandez 2021, Postharvest LED lighting: effect of red, blue and far red on quality of minimally processed broccoli sprouts. J Sci Food Agric 101:44-53. doi:10.1002/jsfa.10820
  10. Chen Y., and S.K.C. Chang 2015, Macronutrients, phytochemicals, and antioxidant activity of soybean sprout germinated with or without light exposure. J Food Sci 80:S1391-S1398. doi:10.1111/1750-3841.12868
  11. de Jong T.J., M.T. Isanta, and E. Hesse 2013, Comparison of the crop species Brassica napus and wild B. rapa: characteristics relevant for building up a persistent seed bank in the soil. Seed Sci Res 23:169-179. doi:10.1017/s0960258513000159
  12. Di Gioia F., M. Renna, and P. Santamaria 2017, Sprouts, microgreens and "baby leaf" vegetables. In Minimally processed refrigerated fruits and vegetables pp. 403-432. Springer, Boston, MA.
  13. Eum H.L., Y. Park, T.G. Yi, J.W. Lee, K.S. Ha, I.Y. Choi, and N.I. Park 2020, Effect of germination environment on the biochemical compounds and anti-inflammatory properties of soybean cultivars. PloS One 15:e0232159. doi:10.1371/journal.pone.0232159
  14. Fei H., Y. Ferhatoglu, E. Tsang, D. Huang, and A.J. Cutler 2009, Metabolic and hormonal processes associated with the induction of secondary dormancy in Brassica napus seeds. Bot 87:585-596. doi:10.1139/b09-022
  15. Fenwick G.R., R.K. Heaney, and W.J. Mullin 1983, Glucosinolates and their breakdown products in food and food plants. Crit Rev Food Sci Nutr 18:123-194. doi:10.1016/0308-8146(83)90074-2
  16. Frankland B., and R. Taylorson 1983, Light control of seed germination. In Photomorphogenesis. pp. 428-456. Springer, Berlin, Heidelberg. doi:10.1007/978-3-642-68918-5_17
  17. Gorelik S., T. Lapidot, I. Shaham, R. Granit, M. Ligumsky, R. Kohen, and J. Kanner 2005, Lipid peroxidation and coupled vitamin oxidation in simulated and human gastric fluid inhibited by dietary polyphenols: Health implications. J Agric Food Chem 53:3397-3402. doi:10.1021/jf040401
  18. Green B.R., and D.G. Durnford 1996, The chlorophyll-carotenoid proteins of oxygennic photosynthesis. Annu Rev Plant Biol 47:685-714. https://doi.org/10.1146/annurev.arplant.47.1.685
  19. Guo X., T. Li, K. Tang, and R.H. Liu 2012, Effect of germination on phytochemical profiles and antioxidant activity of mung bean sprouts (Vigna radiata). J Agric Food Chem 60:11050-11055. doi:10.1021/jf304443u
  20. Hilhorst H.W. 1995, A critical update on seed dormancy. I. Primary dormancy1. Seed Sci Res 5:61-73. doi:10.1017/s0960258500002634
  21. Hoang H.H., C. Bailly, F. Corbineau, and J. Leymarie 2013, Induction of secondary dormancy by hypoxia in barley grains and its hormonal regulation. J Exp Bot 64:2017-2025. doi:10.1093/jxb/ert062
  22. Hollman P.C.H., and M. Katan 1999, Dietary flavonoids: Intake, health effects and bioavailability. Food Chem Toxicol 37:37-942. doi:10.1016/s0278-6915(99)00079-4
  23. Janicki B., B. Kupcewicz, A. Napierala, and A. Madzielewska 2005, Effect of temperature and light (UV, IR) on flavonol content in radish and alfalfa sprouts. Folia Biologica 53:121-125. doi:10.3409/173491605775789272
  24. Jiao C., R. Yang, Y. Zhou, and Z. Gu 2016, Nitric oxide mediates isoflavone accumulation and the antioxidant system enhancement in soybean sprouts. Food Chem 204:373-380, doi:10.1016/j.foodchem.2016.02.147
  25. Jiao Y., O.S. Lau and X.W. Deng 2007, Light-regulated transcriptional networks in higher plants. Nature Rev Genet 8:217-230. doi:10.1038/nrg2049
  26. Josse E.M., and K.J. Halliday 2008, Skotomorphogenesis: the dark side of light signalling. Current Biol 18:R1144-R1146. doi:10.1016/j.cub.2008.10.034
  27. Jung C.H. 2007, Study of growth character and storage for functional sprouts vegetables. MS thesis. Kangwon National Univ. Chuncheon.
  28. Kim E.H., S.H. Kim, J.I. Chung, H.Y. Choi, J.A. Kim, and I.M. Chung 2006, Analysis of phenolic compounds and isoflavones in soybean seeds (Glycine max (L.) Merill) and sprouts grown under different conditions. Eur Food Res Technol 222:201-208. doi:10.1007/s00217-005-0153-4
  29. Koller D., M. Sachs, and M. Negbi 1964, Spectral sensitivity of seed germination in Artemisia monosperma. Plant Cell Physio 5:79-84. doi:10.1093/oxfordjournals.pcp.a079026
  30. Landbo L., and J.B. Jorgensen 1997, Seed germination in weedy Brassica campestris and its hybrids with B. napus: implications for risk assessment of transgenic oilseed rape. Euphytica 97:209-216. https://doi.org/10.1023/A:1003032804340
  31. Lechowska K., S. Kubala, L. Wojtyla, G. Nowaczyk, M. Quinet, S. Lutts, and M. Garnczarska 2019, New insight on water status in germinating Brassica napus seeds in relation to priming-improved germination. Int J Mole Sci 20:540. doi:10.3390/ijms20030540
  32. Lee S.H., C.S. Kim, S.S. Lee, and S.E. Jun 2007, Consumer confidence improving agricultural products. Seoul:Future Agric Policy Res Institute.
  33. Lichtenthaler H.K. 1979, Effect of biocides on the development of the photosynthetic apparatus of radish seedlings grown under strong and weak light conditions. Zeitschrift fur Naturforschung 34:936-940. doi:10.1515/znc-1979-1110
  34. Liu H., Y. Chen, T. Hu, S. Zhang, Y. Zhang, T. Zhao, H. Yu, and Y. Kang 2016, The influence of light-emitting diodes on the phenolic compounds and antioxidant activities in pea sprouts. J Function Food 25:459-465. doi:10.1016/j.jff.2016.06.028
  35. Lucy J.R., S.J. Gro, K.G. Bjorn, and L. Semir 2002, Microbiological analysis of seed sprouts in Norway. Int J Food Microbiol 75:119-126. doi:10.1016/s0168-1605(01)00738-3
  36. Luo Y.W., W.H. Xie, X.X. Jin, Q. Wang, and Y.J. He 2014, Effects of germination on iron, zinc, calcium, manganese, and copper availability from cereals and legumes. CyTA-J Food 12:22-26. doi:10.1080/19476337.2013.782071
  37. Maldini M., F. Natella, S. Baima, G. Morelli, C. Scaccini, J. Langridge, and G. Astarita 2015, Untargeted metabolomics reveals predominant alterations in lipid metabolism following light exposure in broccoli sprouts. Int J Mole Sci 16:13678-13691. doi:10.3390/ijms160613678
  38. Mancinelli A.L. 1985, Light dependent anthocyanin synthesis: A model system for the study of plant photomorphogenesis. Bot Rev 51:107-157. doi:10.1007/bf02861059
  39. Mastropasqua L., N. Dipierro, and C. Paciolla 2020, Effects of darkness and light spectra on nutrients and pigments in radish, soybean, mung bean and pumpkin sprouts. Antioxidants 9:558. doi:10.3390/antiox9060558
  40. McGregor D.I. 1988, Glucosinolate content of developing rapeseed (Brassica napus L. "Midas") seedlings. Can J Plant Sci 68:367-380. doi:10.4141/cjps88-048
  41. Mekenian M.R., and R.W. Willemsen 1975, Germination characteristics of Raphanus raphanistrum. L. Laboratory studies. Bulletin Torrey Bot Club 1975:243-252. doi:10.2307/2484141
  42. Merai Z., K. Graeber, P. Wilhelmsson, K.K. Ullrich, W. Arshad, C. Grosche, D. Tarkowska, V. Tureckova, M. Strnad, S. A-Rensing, G. Leubner-Metzger, and O. Mittelsten Scheid 2019, Aethionema arabicum: a novel model plant to study the light control of seed germination. J Exp Bot 70:3313-3328. doi:10.1093/jxb/erz146
  43. Miller N.J., and C.A. Rice-Evans 1996, Spectrophotometric determination of antioxidant activity. Redox Rpt 2:161-171. doi:10.1080/13510002.1996.11747044
  44. Naeem M.S., D. Liu, R. Raziuddin, G.L. Wan, G.X. Tang, and W.J. Zhou 2009, Seed dormancy and viability. pp 151-176 in Gupta, S.K. (Ed.) Biology and breeding of crucifers. Boca Raton, CRC Press.
  45. Oh M.-M., and C.B. Rajashekar 2009, Antioxidant content of edible sprouts: effect of environmental shocks. J Sci Food Agric 89:2221-2227. doi:10.1002/jsfa.3711
  46. Park W.T., Y.B. Kim, J.M. Seo, S.J. Kim, E. Chung, J.H. Lee, and S.U. Park 2013, Accumulation of anthocyanin and associated gene expression in radish sprouts exposed to light and methyl jasmonate. J Agric Food Chem 61:4127-4132. doi:10.1021/jf400164g
  47. Pekrun C., P.J.W. Lutman, and K. Baeumer 1997, Induction of secondary dormancy in rape seeds (Brassica napus L.) by prolonged imbibition under conditions of water stress or oxygen deficiency in darkness. Eur J Agronomic 6:245-255. doi:10.1016/s1161-0301(96)02051-5
  48. Perez-Balibrea S., D.A. Moreno, and C. Garcia-Viguera 2008, Influence of light on health-promoting phytochemicals of broccoli sprouts. J Sci Food Agric 88:904-910. doi:10.1002/jsfa.3169
  49. Plumb G.W., K.R. Price, M.J. Modes, and G. Williamson 1997, Antioxidant properties of the major polyphenolic compounds in broccoli. Free Radic Res 27:429-435. doi:10.3109/10715769709065782
  50. Pons T.L. 2000, Seed responses to light. In: Seeds: The Ecology of Regeneration in Plant Communities, 2nd edn (ed. M Fenner), 237-260. CAB International, Wallingford.
  51. Riddoch C.H., C.F. Mills, and G.G. Duthie 1998, An evaluation of germinating beans as a source of vitamin C in refugee foods. European J Clinical Nut 52:115-118. doi:10.1038/sj.ejcn.1600524
  52. Samec D., and B. Salopek-Sondi 2019, Cruciferous (Brassicaceae) vegetables. In Nonvitamin and Nonmineral Nutritional Supplements; Nabavi, S.M., Sanches Silva, T., Eds.; Academic Press: Cambridge, MA, USA, pp 195-202.
  53. Samuoliene G., R. Sirtautas, A. Brazaityte, and P. Duchovskis 2012, LED lighting and seasonality effects antioxidant properties of baby leaf lettuce. Food Chem 134:1494-1499. doi:10.1016/j.foodchem.2012.03.061
  54. Smith H.L., L. McAusland, and E.H. Murchie 2017, Don't ignore the green light: exploring diverse roles in plant processes. J Exp Bot 68:2099-2110. doi:10.1093/jxb/erx098
  55. Swieca M., U. Gawlik-Dziki, D. Kowalczyk, and U. Zlotek 2012, Impact of germination time and type of illumination on the antioxidant compounds and antioxidant capacity of Lens culinaris sprouts. Scientia Horticulturae 140:87-95. doi:10.1016/j.scienta.2012.04.005
  56. Taraseviciene Z., H. Danilcenko, E. Jariene, A. Paulauskiene, and M. Gajewski 2009, Changes in some chemical components during germination of broccoli seeds. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 37:173-176.
  57. Thanos C.A., K. Georghiou, D.J. Douma, and C.J. Marangaki 1991, Photoinhibition of seed germination in Mediterranean maritime plants. Annals Bot 68:469-475. doi:10.1093/oxfordjournals.aob.a088280
  58. Vale A.P., J. Santos, N.V. Brito, V. Peixoto, R. Carvalho, E. Rosa, and M.B.P. Oliveira 2015, Light influence in the nutritional composition of Brassica oleracea sprouts. Food Chem 178:292-300. doi:10.1016/j.foodchem.2015.01.064
  59. Vercellino R.B., C.E. Pandolfo, A. Cerrota, M. Cantamutto, and A. Presotto 2019, The roles of light and pericarp on seed dormancy and germination in feral Raphanus sativus (Brassicaceae). Weed Res 59:396-406. doi:10.1111/wre.12377
  60. Vogt T., and P.G. Gul 1994, Accumulation of flavonoids during leaf development in Cistus laurifolius. Phytochem 36:591-597. doi:10.1016/S0031-9422(00)89780-0
  61. Vriet C., E. Russinova, and C. Reuzeau 2013, From squalene to brassinosteroide: the steroid metabolic and signaling pathways across the plant kingdom. Mol Plant 6:1738-1757. doi: 10.1093/mp/sst096
  62. Woolley J.T., and E.W. Stoller 1978, Light penetration and light-induced seed germination in soil. Plant Physiol 61:597-600. doi:10.1104/pp.61.4.597
  63. Yacoubi R., C. Job, M. Belghazi, W. Chaibi, and D. Job 2013, Proteomic analysis of the enhancement of seed vigour in osmoprimed alfalfa seeds germinated under salinity stress. Seed Sci Res 23:99-110. doi:10.1017/s0960258513000093
  64. Yamamuro C., Y. Ihara, X. Wu, T. Noguchi, S. Fujioka, S. Takatsuto, M. Ashikari, H. Kitano, and M. Matsuoka 2000, Loss of function of a rice brassinosteroid insensitive1 homolog prevents internode elongation and bending of the lamina joint. Plant Cell 12:1591-1605. doi:10.1105/tpc.12.9.1591
  65. Yuan M., X. Jia, C. Ding, H. Zeng, L. Du, S. Yuan, Z. Zhang, Q. Wu, C. Hu, and J. Liu 2015, Effect of fluorescence light on phenolic compounds and antioxidant activities of soybeans (Glycine max L. Merrill) during germination. Food Sci Biotechnol 24:1859-1865, doi:10.1007/s10068-015-0243-4