• Journal of Bio-Environment Control
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• v.30 no.4
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• pp.351-358
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• 2021

The study was investigated to compare growth characteristics, the antioxidant activity, and the triterpenoid content of two Centella asiatica cultivars ('Giant Tiger Care' and 'Good Tiger Care'). At 41 days after transplanting, lengths of leaf and petiole were significantly longer in 'Good Tiger Care' than in 'Giant Tiger Care'. However, the growth characteristics (leaf area, petiole thickness, petiole length, and weight) were greater in 'Giant Tiger Care' than in 'Good Tiger Care' at 104 days after transplanting (harvest time). Antioxidant activity and total phenol content in four extracts (WE, water extract; HWE, hot water extract; 50E, 50% EtOH extract; 70E, 70% EtOH extract) of the two cultivars were high in 70E of 'Giant Tiger Care'. As a result of the triterpenoids analysis, the major triterpenoids of the two cultivars were identified as madecassoside and asiaticoside. The total triterpenoid content was high in 50E and 70E of 'Giant Tiger Care' and 'Good Tiger Care', respectively but the total triterpenoid content was highest in Good Tiger Care'. However, at the 104 days after planting, the yield of 'Giant Tiger Care' was three times higher than that of 'Good Tiger Care'. In addition, the total triterpenoid content that can be produced in the same cultivation area (3.3m²) was 2.459mg in 50E of 'Giant Tiger Care', which was ~2.2times higher than that of 70E of 'Good Tiger Care' (1.103 mg). Thus, it is considered economical to cultivate 'Giant Tiger Care' which has the highest antioxidant activity and high total triterpenoid production per cultivation area.

• Korean Journal of Organic Agriculture
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• v.29 no.2
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• pp.223-240
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• 2021

This study was carried out to investigate the economic value of organic wheat production using gelatin·chitin microorganisms in Gwangsan-gu, Gwangju city. The soil condition of experiment field was clay loam Jisan series. The organically cultivated fields were sprayed gelatin and chitin degrading bacteria. The test was performed at conventionally cultivated field and organically cultivated field. Emergence of weed on organically cultivated field was significantly higher than conventionally cultivated field which sprayed herbicide before seeding. Weed emergence have a critical impact on grain yield. Occurrence of diseases and insect pests were higher than conventionally cultivated fields. In 2019, the amount of lodging in conventionally cultivated field were higher than conventionally cultivated field. In 2020, lodging and wet injury were occur in both field. Comparing yield element between organically and conventionally cultivated experimental area, grain yield in organically cultivated field was shown slightly higher amount than conventionally cultivated field. However in the actual yield of 2019, organically cultivated field shows 20% deceased yield because of overgrown weed. In 2020, weed emergence and yellow mosaic virus by wet injury cause 30% decease in the grain yield in organically cultivated field. Content of protein, carbohydrates, ash, water and fat in the grain were not different significance. In 2019, net incomes of conventionally cultivated wheat was 461,031 won/0.1 ha while organically cultivated wheat was 443,437 won/0.1 ha. In the rate of income, conventionally cultivated field was 83.0% as against organically cultivated field (73.3%). In 2020, net incomes of organically cultivated wheat was 437,812 won/0.1 ha while conventionally cultivated wheat was 418,281 won/0.1 ha. In the rate of income, conventionally cultivated field was 81.6% as against organically cultivated field (73.0%).

• Korean Journal of Breeding Science
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• v.49 no.4
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• pp.420-427
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• 2017

'Pungwonmi', a new sweetpotato variety, was developed for table use by Bioenergy Crop Research Institute, National Institute of Crop Science (NICS), RDA in 2014. This variety was derived from the cross between 'Benisatsuma' and 'Luby3074' in 2006. The seedling and line selections were performed from 2007 to 2009, and preliminary and advanced yield trials were carried out from 2010 to 2011. The regional yield trials were conducted at five locations from 2012 to 2014, and it was named as 'Pungwonmi'. This variety has cordate leaf shape, and its leaves, stems, nodes, and petioles are green. Storage root of 'Pungwonmi' has an elliptical shape, red skin, and light orange flesh. 'Pungwonmi' was moderately resistant to fusarium wilt, and resistant to root-knot nematode. Dry matter content was 31.2%, and texture of steamed storage root was intermediate. Total sugar content of raw and steamed storage roots of 'Pungwonmi' was higher than that of 'Yulmi'. ${\beta}$-carotene content of 'Pungwonmi' was 9.1 mg/100g DW. Yield of marketable storage root over 50 g of 'Pungwonmi' was 24.3 MT/ha under the early season culture, which was 46% higher than that of 'Yulmi'. The number of marketable storage roots per plant was 2.8 and the average weight of marketable storage root was 156 g under the optimal and late season culture. Marketable storage root yield of 'Pungwonmi' was 24.1 MT/ha under the optimum and late season culture, which was 26% higher than that of 'Yulmi'. (Registration No. 6428).

• Korean Journal of Breeding Science
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• v.43 no.6
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• pp.600-605
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• 2011

'Boseogheugchal', a new black pericarp glutinous rice cultivar (Oriza sativa L.), was developed by the rice breeding team of National Institute of Crop Science, RDA, Suwon, Korea during the period from 1997 to 2008 and released in 2008. It was derived from a cross between SR18638-B-B-B-18-2/ Heugmi H31. The maturity of this cultivar is about 135 days from seedling to heading. This cultivar has about 12 tillers per hill and 94 spikelet numbers per panicle. Ripening ratio is about 72% and 1000 grain weight is 21.6 g in brown rice. This new variety shows grain shattering resistance and susceptible for blast, bacterial leaf blight and stripe virus. 'Boseogheugchal' has glutinous endosperm and low protein contents. It has higher anthocyanin content compared to black pigmented check cultivar 'Hegjinjubyeo'. The yield performance of brown rice was 4.82 MT/ha in local adaptability test for three years. 'Boseogheugchal' is adaptable to central plain area of Korea.

• Korean Journal of Ichthyology
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• v.30 no.4
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• pp.194-204
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• 2018

In order to minimize the damage on the red sea bream Pagrus major by a harmful dinoflagellate Cochlodinium polykrikoides, we investigated the effect of feeding, starvation and stocking density on the survival rate, growth, growth restoration and physiological response of P. major exposure to C. polykrikoides. The experimental groups were divided into three groups such as F-HD (feeding and high density with $6.4kg/m^3$), S-HD (starvation and high density with $6.4kg/m^3$) and S-LD (starvation and low density with $3.2kg/m^3$) according to stocking density and starvation in marine cage ($11m{\times}11m{\times}5m$). The F-HD was fed throughout the experiment for 9 weeks, whereas S-HD and S-LD were not fed for 5 weeks and then refeeding for 4 weeks. Survival rate was the lowest in F-HD (85.5%) and S-LD was the highest (97.3%). The growth rates of S-HD and S-LD were significantly lower than F-HD during starvation period for 4 weeks, but rapidly recovered after feeding. The nutritional status such as ALB, TP, TCH, TG were similar to tendency of growth data. Ht, Hb, AST, ALT and GLU levels were significantly higher in the F-HD than in the starvation groups at the same time (in 3 week) during starvation period. But starvation groups did not differ during starvation period. As a result, F-HD is more sensitive to stress than S-HD and S-LD. Thus, during C. polykrikoides bloom period, starvation and stocking density control can help survival and growth restoration of the red sea bream.

• Korean Journal of Poultry Science
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• v.46 no.2
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• pp.55-63
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• 2019

This study was conducted to evaluate animal welfare approved farms in three housing systems (open, windowless, and free-range). The survey was conducted in 25 animal welfare approved farms, and 10 farms were surveyed for distribution status. The main breed in all animal welfare approved farms of laying hens was Hy-Line Brown variety. In the case of open house, laying hens were bred in traditional and panel houses simultaneously; however, the ratio of panel house was 58.3%, which was higher than that of the traditional house. All the windowless houses were made of panels and more than 15,000 laying hens were housed in a single windowless house. In the case of free-range house, it was maintained on a small scale of less than 12,000 birds. Fifty-six percent of the surveyed farms were breeding at $7{\sim}8birds/m^2$. In terms of male and female ratios, most farms maintained 1 male:15 females, but there was a farmhouse that switched 17 or 20 females to 1 male. The daily dietary allowance was 110~170 g, and 32% of the surveyed farms provided feed of more than 150 g/day, which showed that forage feed was important. The age of at the first egg was 123 days, 122 days, and 120 days, and the peak percent was 91.8%, 94.9%, and 86.5% in open, windowless and free-range houses, respectively. The average egg production rate was 74.0%, 84.6%, and 72.7% in open, windowless, and free-range houses respectively, thus, there was no correlation between feed intake and hen-housed eggs. Distribution of welfare certified eggs was mainly a direct deal with the consumer or through contract production. The ratio of direct transactions between large-scale marts and eco-friendly specialty stores of welfare approved eggs was higher than that of conventional eggs. The rate of contract sales of eggs in both the barn and free-range systems was high, and the percentage of courier sales farms was also high. Excluding courier services, price of eggs in the barn system rose to more than 30 won/egg in the second half of 2017 (after AI). Price of eggs in the free-range system rose to more than 50 won/egg in the second half of 2017 (after AI). In the case of courier sales, the same price of 500 won was maintained before and after AI. In conclusion, the results of this study can be used as basic data for improving the animal welfare certification system for laying hens in Korea.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.45 no.1
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• pp.9-17
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• 2019

In this study, $L-{\alpha}-amino$ acid, which is a constituent of natural moisturizing factor, was applied to skin in periodic cycle to improve skin tone and texture roughness. Based on the polarity of the alkyl group (R) of the $L-{\alpha}-amino$ acid, the acid was categorized into two groups and their efficacy was studied. As a result, it was found that the improvement rate of $L-{\alpha}-amino$ acid complex with polar alkyl group ($L-{\alpha}-AAC-1$) is 21% higher than that of $L-{\alpha}-amino$ acid complex with non-polar alkyl group ($L-{\alpha}-AAC-2$). For clinical trials, emulsions containing $L-{\alpha}-amino$ acid complex ($L-{\alpha}-AAC-1$) were applied to the randomly selected 20 to 40 year old female participants, as an experimental group, on the right facial cheek once per day for 8 weeks, and emulsions without $L-{\alpha}-amino$ acid complex, as a control group, were applied to the left facial cheek in the same way. Improvements in skin tone were measured using $JANUS^{(R)}$ equipment and analyzed using image analysis software. Skin texture improvement was measured and analyzed mechanically using the phaseshift rapid in-vivo measurement of the skin (PRIMOS) equipment. As a result, improvements of skin tone and skin texture were 11.7% and 6.7%, respectively. In addition, a questionnaire survey was conducted to the participants on the aesthetic improvement and the degree of the feeling of skin improvement. The results suggest that $L-{\alpha}-amino$ acid can be used as a cosmetic substance that can provide aesthetic satisfaction through physiological skin tone and texture roughness.

• Korean Journal of Agricultural and Forest Meteorology
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• v.21 no.1
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• pp.29-41
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• 2019

Terrestrial Gross Primary Production (GPP) is the largest global carbon flux, and forest ecosystems are important because of the ability to store much more significant amounts of carbon than other terrestrial ecosystems. There have been several attempts to estimate GPP using mechanism-based models. However, mechanism-based models including biological, chemical, and physical processes are limited due to a lack of flexibility in predicting non-stationary ecological processes, which are caused by a local and global change. Instead mechanism-free methods are strongly recommended to estimate nonlinear dynamics that occur in nature like GPP. Therefore, we used the mechanism-free machine learning techniques to estimate the daily GPP. In this study, support vector machine (SVM), random forest (RF) and artificial neural network (ANN) were used and compared with the traditional multiple linear regression model (LM). MODIS products and meteorological parameters from eddy covariance data were employed to train the machine learning and LM models from 2006 to 2013. GPP prediction models were compared with daily GPP from eddy covariance measurement in a deciduous forest in South Korea in 2014 and 2015. Statistical analysis including correlation coefficient (R), root mean square error (RMSE) and mean squared error (MSE) were used to evaluate the performance of models. In general, the models from machine-learning algorithms (R = 0.85 - 0.93, MSE = 1.00 - 2.05, p < 0.001) showed better performance than linear regression model (R = 0.82 - 0.92, MSE = 1.24 - 2.45, p < 0.001). These results provide insight into high predictability and the possibility of expansion through the use of the mechanism-free machine-learning models and remote sensing for predicting non-stationary ecological processes such as seasonal GPP.

• Korean Journal of Plant Resources
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• v.32 no.4
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• pp.255-263
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• 2019

Glycyrrhizae radix is one of the most frequently prescribed ingredients in Oriental medicine, and Glycyrrhizae radix extract has been shown to exert anti-cancer effects. However, the cellular and molecular mechanisms of programed cell death (apoptosis) by Glycyrrhizae radix are poorly defined. In the present study, it was examined the molecular mechanisms of apoptosis by water extracts of Glycyrrhizae radix (GRW) in human bladder T24 cancer cells. It was found that GRW could inhibit the cell growth of T24 cells in a concentration-dependent manner, which was associated with the induction of apoptotic cell death, as evidenced by the formation of apoptotic bodies, DNA fragmentation and increased populations of annexin-V positive cells. The induction of apoptotic cell death by GRW was connected with an up-regulation of pro-apoptotic Bax protein expression and down-regulation of anti-apoptotic proteins (Bcl-2 and Bcl-xL), and inhibition of apoptosis family proteins (XIAP, cIAP-1 and cIAP-2). In addition, apoptosis-inducing concentrations of GRW induced the activation of caspase-9, an initiator caspase of the mitochondrial-mediated intrinsic pathway, and caspase-3, accompanied by proteolytic degradation of PARP. GRW also induced apoptosis via a death receptor-mediated extrinsic pathway by caspase-8 activation, resulting in the down-regulation of total Bid and suggesting the existence of cross-talk between the extrinsic and intrinsic pathways. Taken together, the present results suggest that GRW may be a potential chemotherapeutic agent for the control of human bladder cancer cells.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.66 no.1
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• pp.37-71
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• 2021

Rapeseed is a typical winter crop, and its freezing stress tolerance is a major feature for winter survival. Therefore, it is important to comprehend clearly the physical and molecular mechanisms of rapeseed under freezing stress conditions. This study investigates the physical and transcriptome changes of two rapeseed lines, 'J8634-B-30' and 'EMS26', under cold acclimation and freezing temperature treatments. The proline content of 'J8634-B-30' at 5 ℃ increased 8.7-fold compared to that before treatment, and there was no significant change in that of 'EMS26' RNA-sequencing analysis revealed 5,083 differentially expressed genes (DEGs) of 'J8634-B-30' under cold acclimation condition. Among the genes, 2,784 (54.8%) were up-regulated and 2,299 (45.2%) were down-regulated. The DEGs of 'EMS26' under cold acclimation condition were 5,831 genes, and contained 2,199 up-regulated genes (37.7%) and 3,632 down-regulated genes (62.3%). Among them, only DEGs annotated in the cold response-related signaling pathways were selected, and their expression in the two rapeseed lines was compared. Comparative DEGs analysis indicated that cold response related signaling pathways are proline metabolism and ABA (Abscisic acid) signaling. And ICE (Inducer of CBF expression) - CBF (C-repeat-binding factor) - COR (Cold-regulated) signaling were the significantly differentially expressed transcripts in the two rapeseed lines. The major induced transcripts of 'J8634-B-30' induced P5CS (Δ'-pyrroline-5-carboxylate synthetase), which is related to proline biosynthesis, PYL (pyrabactin resistance-like protein, ABA receptor) and COR413 (cold-regulated 413 plasma membrane 1). In conclusion, these result provide a foundation for understanding the mechanisms of freezing stress tolerance in rapeseeds. Further functional studies should be performed on the freezing stress-related genes identified in this study, which can contribute to the transgenic and molecular breeding for freezing stress tolerance in rapeseed.