Journal of Bio-Environment Control (생물환경조절학회지)

The Korean Society for Bio-Environment Control (한국생물환경조절학회)
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Comparison of Growth Characteristics and Physiological Activity of Two Centella asiatica Cultivars in Greenhouse Soil Culture

시설 내 토경재배에서 병풀(Centella asiatica) 두 품종의 생장특성 및 생리활성 비교

  • Oh, Sewon (Division of Animal, Horticultural and Food Sciences, Chungbuk National University) ;
  • Park, Sujeong (Department of Horticultural Science, Gyeongsang National University) ;
  • Lee, Seongho (Department of Horticultural Science, Gyeongsang National University) ;
  • Park, Yeonju (Department of Horticultural Science, Gyeongsang National University) ;
  • Jang, Keum-Il (Department of Food Science and Biotechnology, Chungbuk National University) ;
  • Yu, Kwang-Won (Department of Foold and Nutrition, Korea University of Transportation) ;
  • Kim, Daeil (Department of Horticultural Science, Chungbuk National University) ;
  • Shin, Hyunsuk (Department of Horticultural Science, Gyeongsang National University)
  • 오세원 (충북대학교 축산.원예.식품공학부) ;
  • 박수정 (경상국립대학교 원예과학과) ;
  • 이성호 (경상국립대학교 원예과학과) ;
  • 박연주 (경상국립대학교 원예과학과) ;
  • 장금일 (충북대학교 식품생명공학과) ;
  • 유광원 (한국교통대학교 식품영양학과) ;
  • 김대일 (충북대학교 원예과학과) ;
  • 신현석 (경상국립대학교 원예과학과)
  • Received : 2021.08.31
  • Accepted : 2021.10.15
  • Published : 2021.10.31
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Abstract

The study was investigated to compare growth characteristics, the antioxidant activity, and the triterpenoid content of two Centella asiatica cultivars ('Giant Tiger Care' and 'Good Tiger Care'). At 41 days after transplanting, lengths of leaf and petiole were significantly longer in 'Good Tiger Care' than in 'Giant Tiger Care'. However, the growth characteristics (leaf area, petiole thickness, petiole length, and weight) were greater in 'Giant Tiger Care' than in 'Good Tiger Care' at 104 days after transplanting (harvest time). Antioxidant activity and total phenol content in four extracts (WE, water extract; HWE, hot water extract; 50E, 50% EtOH extract; 70E, 70% EtOH extract) of the two cultivars were high in 70E of 'Giant Tiger Care'. As a result of the triterpenoids analysis, the major triterpenoids of the two cultivars were identified as madecassoside and asiaticoside. The total triterpenoid content was high in 50E and 70E of 'Giant Tiger Care' and 'Good Tiger Care', respectively but the total triterpenoid content was highest in Good Tiger Care'. However, at the 104 days after planting, the yield of 'Giant Tiger Care' was three times higher than that of 'Good Tiger Care'. In addition, the total triterpenoid content that can be produced in the same cultivation area (3.3m2) was 2.459mg in 50E of 'Giant Tiger Care', which was ~2.2times higher than that of 70E of 'Good Tiger Care' (1.103 mg). Thus, it is considered economical to cultivate 'Giant Tiger Care' which has the highest antioxidant activity and high total triterpenoid production per cultivation area.

본 연구는 병풀(Centella asiatica) 두 품종('자이언트 타이거 케어', '굿병풀')의 생장 특성과 추출용매에 따른 항산화 활성 및 Triterpenoid 함량을 조사하기 위해 수행되었다. '자이언트 타이거 케어'와 '굿병풀'의 정식 후 생장 특성은 정식 41일 후 엽과 엽병의 길이 생장에서 '굿병풀'이 유의적으로 높았다. 하지만 이후 수확기(정식 104일 후)까지 생장속도는 유사하였다. 정식 후 104일에 수확한 '자이언트 타이거 케어'의 식물체 크기와 관련된 생장(엽면적, 엽병두께, 엽병 길이, 생체중)이 '굿병풀'보다 유의적으로 높았다. 냉수, 열수, 50% 에탄올, 70% 에탄올 추출물의 자유라디컬 소거능과 환원능으로 평가된 항산화 활성과 총 페놀 함량은 두 품종에서 모두 70% 에탄올 추출물에서 높았다. 추출 용매별 Triterpenoid 함량을 분석한 결과, 두 품종의 주요 Triterpenoid는 Madecasoside와 Asiaticoside로 확인되었다. 품종별 총 Triterpenoids 함량은 '자이언트 타이거 케어'의 50% 에탄올 추출물과 '굿병풀'의 70% 에탄올 추출물에서 가장 높았으며, '굿병풀'이 '자이언트 타이거 케어'보다 높았다. 하지만 정식 104일 후 수확량은 '자이언트 타이거 케어'가 '굿병풀'보다 3배 많아, 동일한 재배 면적(3.3m2) 에서 생산 가능한 총 Triterpenoids 함량은 '자이언트 타이거 케어'가 2.459mg으로 '굿병풀'(1.103mg) 보다 약 2.2배 높았다. 따라서 항산화 활성이 가장 높고 단위면적당 총 Triterpenoids 생산량이 높은 '자이언트 타이거 케어'를 재배하는 것이 경제적일 것으로 생각된다.

Keywords

Acknowledgement

본 논문은 2021년 농촌진흥청 연구사업(세부과제번호: PJ015285052021)의 지원에 의해 이루어진 것임.

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