• Title/Summary/Keyword: 사상과정

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Development of Real-time PCR Assays for Detection of Dirofilaria immitis from Infected Dog Blood (심장사상충에 감염된 개의 혈액에서 심장사상충 유전자를 검출할 수 있는 실시간 중합효소연쇄반응 기법 개발)

  • Oh, In Young;Kim, Kyung Tae;Jun, Jin Hyun;Shin, Jae-Ho;Sung, Ho Joong
    • Korean Journal of Clinical Laboratory Science
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    • v.48 no.2
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    • pp.88-93
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    • 2016
  • Dirofilaria immitis is a filarial nematode parasite that causes cardiopulmonary dirofilariasis in dogs. The purpose of this study was the development of real-time PCR assays for efficient detection of D. immitis. The D. immitis-specific primers confirmed in our previous study and a newly designed TaqMan probe were used for quantitative diagnostics. First, SYBR Green real-time PCR was performed using the specific primers and serially diluted genomic DNA or plasmid DNA, and melting curve analyses were performed after amplification. The melting curve showed one specific peak in each of the genomic and plasmid DNA reactions, suggesting that the primers specifically amplify the D. immitis cytochrome c oxidase subunit I gene. Comparison of SYBR Green and TaqMan real-time PCR using serially diluted plasmid DNA showed higher efficiency and specificity with TaqMan real-time PCR. The real-time PCR assays developed in this study will provide improved diagnostic methods to overcome the limitations of conventional diagnostic tools and facilitate more rapid and accurate diagnoses.

System Development for the Estimation of Pollutant Loads on Reservoir (저수지 유역의 오염부하 산정 시스템 개발)

  • Sim, Sun-Bo;Lee, Yo-Sang;Go, Deok-Gu
    • Journal of Korea Water Resources Association
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    • v.31 no.1
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    • pp.35-44
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    • 1998
  • An integrated system of GIS and water quality model was suggested including the pollutant loads from the watershed. The developed system consists of two parts. First part is the GIS module. The geographic information system of the study area was built to provide the information on landuse and several surface factors concerning the overland flow processes of water and pollutants. Second part is the modeling modules which include storm event pollutant load model(SEPLM)., non-storm event pollutant load model(NSPLM), and river water quality simulation model(RWQSM). Models can calculate the pollutant load from the study area. The databases and models are linked through the interface modules resided in the overall system, which incorporate the graphical display modules and the operating scheme for the optimal use of the system. The developed system was applied to the Chungju multi-purpose reservoir to estimate the pollutant load during the four selected rainfall events between 1991 and 1993,. based upon monthly basis and seasonal basis in drought flow, low flow, normal flow and wet flow.

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Introduction process of 'Corn' and its interrelation with 'Chinese millet' and 'Indian millet' (옥수수(옥촉서(玉蜀黍))의 도입과정과 기장(태(泰)), 수수(촉서(蜀黍))와의 상관관계)

  • Kim, Jong-dug;Koh, Byung-hee;Song, Il-byung
    • Journal of Sasang Constitutional Medicine
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    • v.10 no.2
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    • pp.163-180
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    • 1998
  • In 1492 year, the corn originated in America continent had spread all over the world after spreading into Europe by Columbus. Since the Corn had a similar shape with adlai's one (Yulmoo(율무)), it had been written by the different name of adlai (Yulmoo) at the "訓蒙字會(Hun-mong-ja-hoe)(1527)". Therefore we should consider "Hun-mong-ja-hoe" is the first record of the corn and it is a significant historic record in the civilization exchange between the Orient and the Occident that this record has the only difference of around 30 years later after the spreading corn to Europe. However, this is on the assumption that it is correct for a scholar of Korean literature to persist in that '叡山本' of "Hun-mong-ja-hoe" could be considered as the first edition. The corn had been once classified as a same kind of the Chinese millet because the people had been recognized the corn as a similar group of the Chinese millet and the Indian millet. The Chinese millet contains a summer vigor and becomes as an ingredient of alcoholic drink. And we can find out that $C_4$ type plant (such as corn, Chinese millet, Indian millet, foxtail millet) mostly have a tendency to belong to the food for "Taeumin(太陰人)", because of its high energy efficiency, a flourishing absorption of fertilizing and a strong emission power. The fried corn with a strong summer vigor and a raised feature has a good effect to the treatment of the teethridge disease. And the tea of corn with an warm feature, thanks to its functions of making the stomach and intestines comfortably as well as urination, is a proper food for the 'Taeumin" who is apt to overeat themselves.

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A Study on the medical and pharmacological theory of Interior-Overheated-Disease of Taeumin (태음인(太陰人) 이열병(裡熱病)의 병증(病證) 약리(藥理)에 대한 연구(硏究))

  • Kim, Jong-yeol;Kim, Kyung-yo
    • Journal of Sasang Constitutional Medicine
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    • v.10 no.2
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    • pp.111-150
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    • 1998
  • 1. Background and Purpose: I intended to understand the medical and pharmacological theory of Taeumin, through a study of the process through which Lee Je-ma discovered the Interior-Overheated-Disease of Taeumin and created the prescriptions for it. 2. Methods: I studied and analized the change in the medical and pharmacological theory, through a historical study on the quotations and prescriptions of "DongYi Soose Bowon". 3. Results: Through a literature study I could find that in the existing Oriental Medicine before Lee Je-ma, the difference of the Liver Febrile Disease of Taeumin and the Stomach Febrile Disease of Soyangin were recognized, and the prescriptions of the two diseases were a little distinguished, but the medical theories of those were not distinguished at all. And I found that the Liver Febrile Disease of Taeumin shows the pain in the eyes and the throat, and drying of the nose due to interior dry and heat, so it is different from the Stomach Febrile Disease of Soyangin that shows irritation of fever, headache, and the trouble in passing feces and urine. Also I could know that Radix Puerariae, Rhizoma Cimicifugae, Rhizoma Ligustici, Radix Angelicae Dahuricae, and Radix et Rhizoma Rhei are Taeumin's medicines because these medicines had been used for Taeumin's syndromes, and that Radix Scutellariae, Radix Platycodi, Semen Raphani, and Fructus Gleditsiae are Tae-Eum-In's medicines because these medicines are known as acting for lung.

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An evaluation of the composition and elements in Korean traditional interior space - On Choosa-Gotack in the Chosun dynasty - (한국전통 실내공간의 구성방법과 요소 분석을 통한 의미 고찰 - 조선조 추사고택을 중심으로 -)

  • 천진희
    • Archives of design research
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    • no.16
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    • pp.101-110
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    • 1996
  • CHOOSA GORACK which is a typical example of traditional houses in the CHO SEON dynasty consists of the four terraced builfing group. Among those,SADANG CHAI are located in the highest level,and SADANG CHAI and SADANG CHAI are toward south direction.It means that building orientation and level were influenced by the distinction of social level and the idea of ancester worship. Floor and ceiling level in the same building is different which was caused by the distinetion of social level in the CHO SEON dynasty and an ariental dual as a variety of openings,exposed ceilings,and furniture against a wall were creating a typical Korean visual harmony. Although the furniture and equipments were very important elements in ONDOL BANG the occupance ratio of these were low because the free space in BANG should be utilized effectively to accommodate the space variation.Both an AN CHAI and SARANG CHAI were composed by the standard space module called KAN.And interior elements were established by several factors such as the human scale,the behavior pattern in traditional sitting life style, and the lumber size of post and lintel construction of Korean house.BANG and DAE GHUNG, composed of KAN,were expanded and arranged side by side so that the natural light and ventilation through them could be used in the result of the kind of lay out,traffic circulation was disturved.In conclusion,CHOOSA GOTACK was formed by the sirect effice of the KOREAN penisula. However this study was based on one sample. It may not enough to deduct soild conclusion.Therefore continuous and farher study is needed for the sestemane evaluation.

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Evaluation of the Clark Unit Hydrograph Parameters Depending on Basin and Meteorological Condition: 2. Estimation of Parameter Variability (유역 및 기상상태를 고려한 Clark 단위도의 매개변수 평가: 2. 매개변수의 변동성 추정)

  • Yoo, Chul-Sang;Lee, Ji-Ho;Kim, Kee-Wook
    • Journal of Korea Water Resources Association
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    • v.40 no.2 s.175
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    • pp.171-182
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    • 2007
  • In this study, as a method for decreasing the confidence interval of the estimates of Clark hydrograph's concentration time and storage coefficient, regression equations of these parameters with respect to those of rainfall, meteorology, and basin characteristics are derived and analyzed using the Monte Carlo simulation technique. The results are also reviewed by comparing them with those derived by applying the Bootstrap technique and empirical equations. Results derived from this research are summarized as follows. (1) Even in case of limited rainfall events are available, it is possible to estimate the mean runoff characteristics by considering the affecting factors to runoff characteristics. (2) It is also possible to use the Monte Carlo simulation technique for estimating and evaluating the confidence intervals for concentration time and storage coefficient. The confidence intervals estimated in this study were found much narrower than those of Yoo et al. (2006). (3) A supporting result could also be derived using the Bootstrap technique. However, at least 20 independent rainfall events are necessary to get a rather significant result for concentration time and storage coefficient. (4) No empirical equations are found to be properly applicable for the study basin. However, empirical equations like the Kraven(I) and Kraven(II) are found valid for the estimation of concentration time, on the other hand the Linsley is found valid for the storage coefficient In this study basin. But users of these empirical formula should be careful as these also provide a wide range of possible values.

Dual Structure of the Theory of 'Tao' in East Asia (동아시아 도론(道論)의 이중구조 탐색 - '도'와 '길'의 변증법적 길항(拮抗) 관계 -)

  • Jang, Yun-su
    • Journal of Korean Philosophical Society
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    • v.146
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    • pp.245-270
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    • 2018
  • In this paper I study the theoretical characteristic of 'Tao' based on Confucianism. Tao, the most widely used keyword in the history of East Asian philosophy, has amassed ever-greater variety of meaning as time has passed and schools divided into more subdivided schools. Among the many attributes of Tao, the most important thing I think is that Tao has a dual structure between 'Tao' and 'Way'. Somewhere to be reached is called 'Tao', and the pathway that leads to the destination is called 'Way'. In other words, 'Tao' is existence itself, and 'Way' is type of existence. 'Tao' can be goal, and 'Way' can be process. 'Tao' has originality, continuance, and honesty as its own traits, while 'Way' has humanity, practice, and dailiness as its traits. After all, in a broad sense, Tao has a dual structure between the existence and the type of existence; 'Tao' and 'Way'. Many philosophers in Western society have also paid attention to this Tao trait. Among them, Heidegger gave his opinion that is closest to the East Asians. I want to explain why the human beings are the existence on the pathway through this paper. The existence(Tao) and the type of existence(Way) cannot be separated. So, there cannot be existence without type, and type of non-existence is useless. From this point of view, 'Tao' can be both existence and type of existence.

Estimating on the Erosion and Retreat Rates of Sea-cliff Slope Using the Datum-point in Pado-ri, the Western Coast of Korea (침식기준목을 이용한 파도리 해식애 사면의 침식·후퇴율 산정)

  • JANG, Dong-Ho;PARK, Ji-Hoon
    • Journal of The Geomorphological Association of Korea
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    • v.19 no.3
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    • pp.71-82
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    • 2012
  • This research was carried out to estimate annual erosion and retreat rates by using datum-point and to identify the characteristics and causes of seasonal variations of sea-cliff slope in Pado-ri, Taean-gun. In the result, the erosion and retreat rates of sea-cliff were increased from spring to summer. The rates were increased rapidly between August and October, caused by the effects of extreme weather events such as severe rainstorms and typhoons, etc. Since then, the erosion and retreat rates of sea-cliff were decreased gradually, but the rates were increased again in winter due to the storm surge and mechanical weathering resulting from the repeated freezing and thawing actions of bed rocks. The factors that affect erosion and retreat rates of sea-cliff include the number of days with antecedent participation and daily maximum wave height. In particular, it turned out that the erosion is accelerated by strong wave energy during storm surges and typhoons. The annual erosion and retreat rates of study area for the past two years(from May 2010 to May 2012) were approximately 44~60cm/yr in condition of differences in geomorphological and geological characteristics at each point. These erosion and retreat rates were found to be higher than results of previous researches. This is caused by coastal erosion forces strengthened by extreme weather events. The erosion and retreat process of sea-cliff in the study area is composed by denudation of onshore areas in addition to marine erosion(wave energy).

Study on G. Deleuze's "Francis Bacon: The Logic of Sensation" - Focus Primarily on Concept of 'Figure' - (들뢰즈의 『감각의 논리』에 관한 연구 - '형상'개념을 중심으로 -)

  • Jin, Gi-haeng
    • Journal of Korean Philosophical Society
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    • v.141
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    • pp.263-286
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    • 2017
  • It is well known to us that in its enlarged sense Deleuze's philosophy has confrontation with platonism that has dominated the whole western ideas, and in a narrow sense it is confrontation with representative thinking and representative arts. In other words, it's obvious what Deleuze has considered the most important in his whole of ideas is to think not in a representative way but in a non-representational way and to disentangle thinking from its representative image. To examine the way how Deleuze criticize representation, and how he overcome modern ideas is not just to make a clean breast of platonic inheritance. Because they are essential for facing up to the actual circumstances of our contemporaries who have degraded servility of totalitarian thoughts under the thinking of identity, and furthermore, it is essential for overcoming this situation and proceeding to nomadic thinking, liberating thinking. This study is not intended to be a definitive account of all his criticism of representation. Because, as is well known, Deleuze's criticism of representation contains a wide variety. And so this study were limited a relatively small number of points in Deleuze's position on representation as follows. How does Deleuze's criticism of representation has been developed in his theory of paintings? And what does it mean to us today? In this paper, I paid special regard to make sense out Deleuze's concept of 'figure' in his important writings that pertains to an analysis of his criticism of representation, Francis Bacon: The Logic of Sensation. And having traced the development process of the concept of 'figure', I want to understand the implicit meaning of the concept in constant flux of his critical thinking.

Historical Contemplation on the Korean Landscape Characteristics as Affected by Religious Environment (시대 및 종교적 환경과 한국의 조경 경관형성 소고)

  • Shim, Jai-Sung;Bae, Jeong-Kwan;Seo, Byung-Key;Choi, Jong-Myung
    • The Journal of Natural Sciences
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    • v.12 no.1
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    • pp.85-101
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    • 2002
  • Landscape civilization in Korea originated in Cochosun(Ancient Chosun) era, this again succeeding to the period of the Three States - Koguryo, Baekje and Silla. The distribution of this culture showed great progress with the association of two particular religions - Buddhism and Confucianism.. Landscape development in Korea has greatly changed during specific times of both cultural and political upheaval in various societies. Religion has had a great deal of influence on landscape development. Traditionally Korean people have had a tendency to favor more natural landscape than man-made structures in landscape : This trend was a quite different concept from that of other oriental countries, not to mention of western countries. In particular, Buddhism influenced natural landscape, far from artificial craftsmanship in landscape. Oriental garden is a typical 'tabloid edition' of natural landscape which consists lakes, islands, ponds, stone monuments, and fruit trees, quite often raising animal in parks and courtyard style house. This style of garden influenced in Chosun Dynasty landscape. Landscaping was usually for royal gardens, cemetery parks or high level of officer's residence. However, landscaping in Chosun Dynasty which had established Confucianism as a state religion gave us a specific designation. It was neither ethnic imitation of the garden style of both China and Japan : People were used to enjoy nature-friendly landscape or sink into the ecstasy of natural scenery itself. The trend that landscape or establishing garden had been aimed at royal family- or bureaucrat-centered formatives was to become an obstacle to the development of landscape techniques in Korea. An example represented in a beautiful garden with fabulous decoration which established in places. This was completely not fit for the nation's feeling.

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