• Title/Summary/Keyword: 분해기법

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효율적인 Bioventing 공정을 위한 모니터링 시스템 개발

  • 문원재;오영진;왕성환;박태주
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2002.09a
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    • pp.359-361
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    • 2002
  • 국내에서는 1990년대 이후부터 주유소 및 유류저장시설 부근의 토양 및 지하수오염에 관한 법이 제정되었으며 그와 관련된 복원 및 정화 기술개발에 관한 연구가 진행되고 있다. 그 중 미생물학적 대사를 이용하는 생물학적복원기술(Bioremediation)은 유류로 오염된 지역에서 정화 및 복원방법으로 적용되고 있으며 또한 경제적인 대안으로 제시되고 있다. Bioremediation기법중 하나인 bloventing은 운전 및 처리비용의 감소와 효율의 향상적인 측면에서 여러 연구자들에 의해 수행되고 있다. 기존에는 오염토양에 대한 물리ㆍ화학적 분석과 미생물 활성에 미치는 인자의 측정이 주를 이루었다. 그러나, 최근에는 토양 가스내 $CO_2$ $O_2$의 측정ㆍ분석을 통한 분해율 평가와 탄화수소 분해에 대한 경과 및 복원시간을 예측하고자 하는 연구가 이루어지고 있는 중이다. 본 연구의 목표는 유류오염지역을 복원하기 위한 기술 중 생물학적 복원기술(bioremediation)의 하나인 bioventing 기술의 국내 적용가능성을 알아보고 궁극적으로 국내 실정에 적합한 bioventing공법의 개발을 목표로 한다. 이를 위해 lab-scale bioventing process를 이용하여 생부해를 최대화시키고 오염성분의 휘발을 최소화시키는 bioventing system의 최적운전 mode(연속식, 간헐식)를 개발하기 위해 공기공급량과 생분해량, 그리고 휘발되는 탄화수소양과의 관계를 평가하는 연구를 수행하였다.

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Parallel Processing Based Decompositon Technique for Efficient Collaborative Optimization (효율적 분산협동최적설계를 위한 병렬처리 기반 분해 기법)

  • Park, Hyeong-Uk;Kim, Seong-Chan;Kim, Min-Su;Choe, Dong-Hun
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.25 no.5
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    • pp.883-890
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    • 2001
  • In practical design studies, most of designers solve multidisciplinary problems with large size and complex design system. These multidisciplinary problems have hundreds of analysis and thousands of variables. The sequence of process to solve these problems affects the speed of total design cycle. Thus it is very important for designer to reorder the original design processes to minimize total computational cost. This is accomplished by decomposing large multidisciplinary problem into several multidisciplinary analysis subsystem (MDASS) and processing it in parallel. This paper proposes new strategy for parallel decomposition of multidisciplinary problem to raise design efficiency by using genetic algorithm and shows the relationship between decomposition and multidisciplinary design optimization (MDO) methodology.

Analysis of Nitrophenols Using Liquid Chromatography/Atmospheric Pressure Chemical Ionization Mass Spectrometry (액체 크로카토그래피-대기압화학이온화법 질량분석기를 이용한 nitrophenol류의 분석)

  • Hong, Jongki;Yoo, Jong Shin;Kim, Kang-Jin
    • Analytical Science and Technology
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    • v.10 no.1
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    • pp.9-17
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    • 1997
  • Nitrophenols are a group of important priority pollutants classified by the US Environmental Protection Agency. Reverse-phase liquid chromatographic method combined with mass spectromethy employing atmospheric pressure chemical ionization(APCI) interface is utilized to determine a mixture of nitrophenols in water matrix without any pretreatment. The sensitivity and selectivity for the identification of different kind of nitrophenols is enhanced by the use of selected ion monitoring and cone voltage fragmentation. The fragmentation patterns of nitrophenols are compared with those obtained from the collision induced dissociation(CID) MS/MS technique.

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Forged Color Region Detection Using Color Pattern Decomposition and Hypothesis Test (컬러 패턴의 분해와 가설검정을 이용한 컬러 조작 영역 검출)

  • Seo, Jun Ryung;Eom, Il Kyu
    • Journal of the Institute of Electronics and Information Engineers
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    • v.52 no.7
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    • pp.77-85
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    • 2015
  • In this paper, we present a new method that can detect forged color region using color pattern decomposition and hypothesis testing approach. On the basis of the fact that the variance of the interpolated pixel is smaller than that of the original pixel, we use a statistical test method to judge the statistical inconsistency of variance. For this, we calculate the variance adopting a color pattern decomposition according to the demosaicking pattern. In addition, we apply high-pass filtering to enlarge the difference between the variances of original and interpolated pixel. Through experimental simulations, we can see that our proposed method can effectively detect forged color regions and shows superior detection performance compared to the conventional method.

Study on Physicochemical Properties of Pesticide. (I) Water Solubility, Hydrolysis, Vapor Pressure, and n-Octanol/water Partition Coefficient of Captafol (농약의 물리화학적 특성연구 (I) Captafol의 수용성, 가수분해, 증기압, 옥탄올/물 분배계수)

  • Kim, Jeong-Han;Lee, Sung-Kyu;Kim, Yong-Hwa;Kim, Kyun
    • Applied Biological Chemistry
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    • v.40 no.1
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    • pp.71-75
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    • 1997
  • Important physicochemical properties of captafol [N-(1,1,2,2-tetrachloro-ethylthio)cyclohex-4-ene-1,2-dicarboximide], water solubility, vapor pressure, hydrolysis and octanol/water partition coefficient(Kow) were measured based on the standard EPA and OECD methods. Water solubility of the chemical was 2.24 ppm at $25^{\circ}C$. Half-life by hydrolysis at $25^{\circ}C$ in the buffer solution of pH 3.0, pH 7.0, and pH 8.0 was 77.8 hr, 6.54 hr and 0.72 hr, respectively, demonstrating instability in alkaline solution. The half-life in acid condition was not significantly different by temperature change, however, that in neutral or alkaline solution became shorter at $40^{\circ}C$. Hydrolysis study with a reference compound, diazinon, proved that the experimental method of the present study is reliable. Vapor pressure of captafol, $8.27{\times}10^{-9}$ torr at $20^{\circ}C$, was calculated from the equation, log P=6.94-(4401.6/T) plotted on the experiment results under different temperature conditions, 40, 50, and $60^{\circ}C$. pressure of captafol, the contamination of captafol would not happen easily in environment by vaporization. High Kow value of 1,523 was observed and this might result in bioconcentration through food chain when captafol was exposed. However, affecting human health through aquatic bioaccumulation is not likely to occur due to its rapid hydrolysis in the environment.

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Strain Development for the Over-production of Alkaline Protease from Vibrio metschnikovii by Molecular Evolution (분자진화 기술을 통한 Vibrio metschnikovii 유래 고활성 알칼리성 단백질 분해효소 생산균주 개발)

  • Shin, Yong-Uk;Lee, Gwa-Soo;Jo, Jae-Hyung;Lee, Hyune-Hwan
    • Korean Journal of Microbiology
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    • v.46 no.4
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    • pp.383-388
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    • 2010
  • Alkaline protease-overproducing strains of Vibrio metschnikovii were developed by using the molecular evolution from the classical mutants V. metschnikovii L12-23, N4-8, and KS1. Each vapK (Vibrio alkaline protease K) was obtained from the genomic DNAs of mutants by PCR to carry out the DNA shuffling. The modified vapK-1 obtained by DNA shuffling was used again as a template for the error-prone PCR to make the vapK-2. Both genes were cloned in the plasmid pKF3 to construct the recombinant plasmids which have one or two copies of the modified genes. The recombinant plasmids were back-transformed to V. metschnikovii KS1 to construct recombinant V. metschnikovii that expresses the alkaline protease. About 3.9-fold more protease activity was measured in the strain which has the plasmid containing two copies of vapK-2 when compared to strain KS1. When compared to wild type V. metschnikovii RH530, 43-fold more activity was achieved. Comparison of amino acids among vapK, vapK-1, and vapK-2 revealed that the active sites was highly conserved and not changed. However, many amino acids except the active sites were changed. These results suggested that the changes in amino acids might play an important role in the increase of protease activity by allowing the easy access of substrate to active sites of the protease. The fermentation of alkaline protease from the V. metschnikovii KS1 harboring the plasmid that contains two copies of vapK-1 showed the possibility of this strain to be used as industrial producer.

A broadband detection algorithm using cross-correlation of two split beams for cylindrical array sonar (원통형 배열 소나를 위한 두 개의 분리 빔의 상호상관을 이용한 광대역탐지 기법)

  • Kwak, ChulHyun
    • The Journal of the Acoustical Society of Korea
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    • v.36 no.5
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    • pp.300-304
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    • 2017
  • In a cylindrical sonar, a conventional broadband energy detector has limitations in the separation of adjacent targets. In this paper, a broadband detection algorithm using cross-correlation is applied to the cylindrical sonar to improve the bearing resolution. The proposed algorithm uses split beamforming before broadband detection processing using cross-correlation to generate half beams. The time delay obtained from the peak of correlation between half beams is used to estimate the bearing of target. Simulations demonstrate the improved performance of the proposed algorithm against the conventional algorithm.

Audio Watermarking in Sub-band Signals Using Multiple Echo Kernel (다중 반향 커널을 이용한 부대역 신호 기반 오디오 워터마킹)

  • Oh In-Jung;Cho Jae-Won;Chung Hyun-Yeol;Jung Ho-Youl
    • Proceedings of the Acoustical Society of Korea Conference
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    • spring
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    • pp.355-358
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    • 2004
  • 최근 웨이블릿 변환을 이용하여 저주파수 부대역 신호를 반복적으로 분해하고 삽입된 워터마크 비트에 따라 특정 부대역 신호에 두개의 다른 반향 중 하나를 삽입하는 반향 삽입 기법을 제안하였다. 이 기법은 오디오 신호의 주파수 특성을 고려했기 때문에 시간 영역에서의 기존의 반향 삽입 기법에 비해 비가청성(inaudibility), 워터마크의 강인성(robustness) 및 용적성(capacity)을 모두 증가시킬 수 있다. 기존의 부대역 신호 기반 반향 삽입 기법이 단일 반향 커널만을 이용하였으나, 본 논문에서는 부대역 신호에 다중 반향 커널을 이용한 반향 삽입 기법을 제안한다. 이 기법은 주관적 왜곡(perceptibility)과 SNR(signal to Noise Ratio)과 같은 객관적인 왜곡 면에서, 원본 오디오 신호의 왜곡을 줄일 수 있다. 실험 결과를 통하여 기존의 방법에 비해 제안된 방법이 SNR과 DR(Detection Rate) 모두 좋은 성능을 보여 제안된 방법의 유효성을 입증하였다.

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Soft X-ray Nanoscopy for Nano- and Bio-materials at the Pohang Light Source

  • Kim, Nam-Dong
    • Proceedings of the Korean Vacuum Society Conference
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    • 2016.02a
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    • pp.86-86
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    • 2016
  • 최근 포항가속기연구소 10A 빔라인에 Scanning Transmission X-ray Microscopy (STXM)가 완성되어 운영 중이다. Soft x-ray imaging 장치로서 기술적으로 Sample scanning 기법이 활용된다. 이는 Zone plate를 통해 집속된 빔이 샘플에 조사되고 검출되는 방식이다. 이러한 Scanning 기법을 활용하고 있는 10A STXM은 기본적으로 흡수분광기법 (x-ray absorption spectroscopy)을 이용하고 있다. 특히, 10A 빔라인 STXM은 최고 20 nm까지 공간분해능이 가능하다는 장점이 있다. 따라서 수십에서 수백 나노미터 크기의 시료들 또는 나노구조에 대한 물리화학적 상태 분석이 쉽게 이루어지고 있다. 주로 시료를 투과하면서 흡수되는 X-선 세기 대비를 맵핑하는 형식의 이미지 데이터와 더불어 X-선의 에너지를 조정함으로써 각 에너지에 해당하는 이미지스택을 결과로 얻게 된다. 이러한 이미지 결과로부터 시료의 나노크기에서 오는 물리화학적 상태를 분석하고 물리에서 바이오까지 다양한 분야의 실험 활용이 가능한 상태다.

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Formal tests for State-model based Specifications on Software Components (상태모델에 기반한 소프트웨어 컴포넌트 명세의 정형적 테스트)

  • Seo, Dongsu
    • The Journal of Korean Association of Computer Education
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    • v.7 no.6
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    • pp.129-139
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    • 2004
  • In developing highly reliable systems such as C4I systems formal methods provide both developers and clients with assurance that they are in the right development processes. This paper investigates into techniques for formal specifications and tests for software components where rigorous verification is required. In particular, the paper suggests decomposition techniques for state-model based specifications using the weakest precondition, and suggests test methods for the specification by generating black box test-cases.

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