• Korean Journal of Poultry Science
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• v.32 no.1
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• pp.9-14
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• 2005

This study was conducted to investigate the effects of Bacillus subtilis on the egg qualify, blood characteristics and fecal $NH_3-N$ in laying hens. A total of two hundred fifty two laying hens were randomly allocated into three treatments with seven replications for six weeks. Dietary treatments included 1) CON (control; basal diet), 2) BS0.2(control + $0.2\%$ Bacillus subtilis) and 3) BS0.4 (control +$0.4\%$ Bacillus subtilis). For overall Period, hen-day egg production tended to increase by the Bacillus subtilis $0.4\%$ in the diets, but was no significant difference. Egg weight, egg shell breaking strength, egg shell thickness, Haugh Unit, yolk color unit and egg yolk index were not affected by treatments. Difference of egg weight and egg shell breaking strength in the BS0.2 treatment tended to increase without significant difference. Difference of egg yolk index in laying hens fed Bacillus subtilis was increased (P<0.05). The concentrations of red blood cell (RBC) and white blood cell (WBC) were not significant difference. $NH_3-N$ concentration in feces with BS0.4 treatment was significantly (P<0.05) lower than control. In conclusion, dietary Bacillus subtilis could decrease fecal $NH_3-N$.

• Journal of Animal Science and Technology
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• v.48 no.3
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• pp.375-382
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• 2006

This study was conducted to investigate the effects of dietary herbal plant mixture on growth performance, blood immunological parameters, fecal VFA and NH3-N concentrations in growing pigs. The dietary treatments were 1) NC (negative control; antibiotics-free diet), 2) PC (positive control; NC diet added 0.16% antibiotic), 3) NCK0.2 (NC diet added 0.2% herbal plant mixture (koppuul??)) and 4) PCK0.1 (PC diet added 0.1% herbal plant mixture (koppuul??)). Eighty crossbred (Landrace×Yorkshire×Duroc) pigs (16.35±0.05kg average initial body weight) were used for 42 days. The pigs were assigned to the treatments according to body weight and each treatment had 5 replicates of 4 pigs per pen in a randomized complete block design. During the whole experimental period, PCK0.1 and PC treatments had significantly higher ADG than NC treatment (P<0.05). Gain/feed of pigs fed PC diet was higher than that of pigs fed NC diet (P<0.05). For blood immunological parameters investigations, white blood cells (WBC) counts and IgG level increased in the pigs fed PC, NCK0.2 and PCK0.1 diets compared to pigs fed NC diet. In addition, higher (P<0.05) serum lymphocyte concentration was found in PCK0.1 treatment compared to NC treatment. NH3-N concentration in pigs fed NCK0.2% diet was lower than that in pigs fed PC and NC diets. There were no significant differences in DM and N digestibilities among the treatments. In conclusion, the results suggested that the dietary additions of herbal plant mixture or antibiotics increase ADG, the concentrations of WBC counts and IgG, lymphocyte, while decrease NH3-N concentration in feces.

• Korean Journal of Poultry Science
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• v.29 no.1
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• pp.1-6
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• 2002

This study was conducted to investigate the effects of feeding probiotics on the growth performance, blood composition, and fecal noxious gas in broiler chickens. Two hundred eighty eight broiler chickens were randomly allocated into three treatments with eight replications for live weeks. Dietary treatments included 1) Control (basal diet), 2) PB 0.5 (basal diet +0.5% probiotics), 3) PBI.0 (basal diet + 1.0% Probiotics for 0 to 1 wk of age, 0.5% probiotics for 1 to 3 wk of age, 0.25% probiotics for 3 to 5 wk of age) . There were no significantly difference among treatments for the body weight gain, but the feed intake of control group significantly (p<0.05) increased as compared with the PBI.0 group in overall experimental period. Digestibility of DM in PB0.5 group (80.84%) was significantly (p<0.05) improved as compared with those in PB 1.0 (79.34%) or control group (79.15%). Although N digestibility was slightly higher in broiler chicks fed probiotics than Control, there was no significance between probiotic groups. Total cholesterol, triglyceride, HDL- and LDL-cholesterol were not significantly different among the treatments. NH$_3$-N concentration in feces with PBI .0 treatment was lower (p<0.05) than Control or PB 0.5 treatments. These results indicated that locally obtained probiotics can be effectively used for reduction of ammonia nitrogen.

• Journal of Animal Science and Technology
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• v.45 no.5
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• pp.787-796
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• 2003

Two experiments were conducted to investigate the effect of germanium biotite (GB) on growth performance, nutrient digestibility and fecal gas emission in pigs. In Exp 1., a total of one hundred nursery pigs (initial body weight 13.12${\pm}$0.15kg) were used in a 21 d growth assay. The five treatments were control (CON, basal diet), GB0.5-200 (basal diet + 0.5% GB, 200mesh), GB1.0-200 (basal diet + 1.0% GB, 200mesh), GB0.5-325 (basal diet + 0.5% GB, 325mesh), GB1.0-325 (basal diet + 1.0% GB, 325mesh). Each treatment had four replicates with five pigs per replicate. ADG, ADFI and gain/feed were not significantly different among the treatments. Fecal NH3-N concentration of pigs fed the GB325 diet was lower than that of pigs fed the GB200 diet (P=0.01). The GB treatments reduced fecal volatile fatty acids significantly compared to the CON (propionic acid, P=0.01; butyric acid, P=0.01; acetic acid, P=0.02). Especially, fecal propionic acid concentration of pigs fed the GB325 diets was lower than that of pigs fed the GB200 diets (P=0.02). In Exp 2., a total of seventy five pigs (initial body weight 21.18${\pm}$0.15kg) were used in a 28 d growth assay. The treatments were same as described for Exp. 1. Each treatment had five replicates with three pigs per replicate. The GB1.0 treatments significantly increased the ADG compared to the GB0.5 treatments (P=0.03). The DM and N digestibility of pigs fed the GB1.0 diets were higher than that for pigs fed the GB0.5 diets (P=0.01). Also, the Ca digestibility of pigs fed the GB diets was higher than that for pigs fed the CON diets (P=0.01). The fecal NH3-N concentrations for the GB treatments were lower than that for the CON (P=0.01). The GB325 treatments significantly decreased the fecal NH3-N concentration compared to the GB200 treatments (P=0.03). The fecal butyric acid concentration for the GB325 treatments was lower than that for the GB200 treatment (P=0.04). In conclusion, the results obtained from these feeding trials suggest that the dietary GB for nursery pigs affects fecal noxious gas emission. In growing pigs, dietary GB was effective to improve ADG and decrease fecal noxious gas emission.

• Korean Journal of Poultry Science
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• v.30 no.1
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• pp.61-66
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• 2003

This study was conducted to investigate the effects of dietary germanium biotite supplementation on egg quality and fecal noxious gas content in laying hens. One hundred and forty-four 40-wk-old, ISA Brown layers were used in this experiment. Dietary treatments were 1) CON(control diet), 2) GB0.5(control diet + 0.5% germanium biotite), 3) GB1.0 (control diet + 1.0% germanium biotite) and 4) GB1.5(control diet + 1.5% germanium biotite). Hen-day egg Production and egg shell breaking strength were not influenced by germanium biotite supplementation. However, egg weight decrease as the level of germanium biotite supplementation increased in the diets increased(Cubic effect, p<0.02). Eggshell thickness, yolk color and yolk index were not influenced by garmanium biotite supplementation. Serum triglyceride concentration as dietary germanium biotite increased (linear effect P<0.02; quadratic effect, p<0.05). Dietary supplementation of germanium biotite reduced fecal NH$_3$-N concentration (P<0.01), propionic acid(P<0.01), butyric acid( P<0.05) and acetic aicd(P<0.01) concentrations in the feces. In conclusion, the results of this experiment indicated that dietary germanium biotite supplementation did not affect egg shell quality, but reduced fecal NH$_3$-N concentration.

• Journal of Animal Science and Technology
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• v.47 no.6
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• pp.937-946
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• 2005

This experiment was conducted to investigate the effects of dietary Bacillus subtilis on growth performance, nutrient digestibility, immunological cells change, fecal noxious gas and carcass meat quality characteristics in finishing pigs. The dietary treatments were 1) CON(basal diet), 2) BS0.1(basal diet+ 0.1% Bacillus subtilis) and 3)BS0.2(basal diet+0.2% Bacillus subtilis). Sixty crossbred(Landrace$\time$ Yorkshire$\time$Duroc) pigs(89.5$\pm$0.11kg average initial body weight) were used in a 42 days growth trial. The pigs were assigned to the treatments according to body weight and each treatment had 5 replicates of 4 pigs per pen in a randomized complete block design. Through the entire experimental period, average daily gain(ADG) and average daily feed intake(ADFI) were not significantly different among the treatments. Pigs fed BS0.1 diet significantly increased their gain/feed compared to pigs fed CON and BS0.2 diets(P<0.05). Also, dry matter(DM) and nitrogen(N) digestibilities were greater in the pigs fed BS0.1 diet than those fed CON diet(P<0.05). There were no significant differences in fecal NH3-N concentration among the treatments. In blood assay for immunological cells change investigations, red blood cells(RBC) counts increased in the pigs fed BS0.2 diet compared to pigs fed CON and BS0.1 diets. There were no significant differences in carcass pH, drip loss, marbling and firmness. However, sensual color and a*(redness) value of meat in the pigs fed BS0.2 diet were higher than in pigs fed CON diet(P<0.05). Therefore, this experiment suggested that Bacillus subtilis supplementation could improve nutrient digestibility, RBC counts and carcass meat color of pigs.

• Journal of Animal Science and Technology
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• v.44 no.3
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• pp.305-314
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• 2002

For the Exp. 1, a total of seventy two pigs (10.53${\pm}$0.02kg average initial body weight) were used in a 38-d growth assay to determine the effects of Saccharomyces cerevisiae (SC) supplementation on growth performance and fecal microbial populations. Dietary treatments included 1) CON (corn-dried whey-SBM based diet), 2) SC0.2 (CON diet+0.2% SC) and 3) SC0.4 (CON diet+0.4% SC). Through the entire experimental period, ADG, ADFI and gain/feed were not significantly different among the treatments. At d 7 and 14 after the onset of the experiment, fecal Lactobacilli sp. count increased as the concentration of SC in the diets was increased (linear effect, P$<$0.01). At d 7 after the onset of the experiment, fecal Escherichia coli count decreased as the concentration of SC in the diets was increased (linear effect, P$<$0.02, quadratic effect, P$<$0.03). For the Exp. 2, forty five pigs (49.71${\pm}$0.45kg average initial body weight) were used in a 28-d growth assay to determine the effects of complex probiotics (CPB, Phichia anomala ST, Galactomyces geotrichum SR59, Thiobacillus sp.) supplementation on growth performance, nutrient digestibility and fecal $NH_3$-N and volatile fatty acid concentrations. Dietary treatments included 1) CON (corn-SBM based diet), 2) CPB0.2 (CON diet+0.2% CPB) and 3) CPB0.3 (CON diet+0.3% CPB). Through the entire experimental period, pigs fed CPB0.3 diet significantly increased their ADG compared to pigs fed CON and CPB0.2 diets (P$<$0.05). Also, apparent digestibility of DM and N in pigs fed CPB0.3 diet was greater than for pigs fed CON diet (P$<$0.05). Fecal $NH_3$-N decreased (P$<$0.05) in the pigs fed CPB diet compared to pigs fed CON diets. Also, pigs fed CPB0.3 diet significantly decreased their fecal propionic acid compared to pigs fed CON diets (P$<$0.05). In conclusion, the results obtained from these feeding trials suggest that the dietary SC for nursery pigs affects fecal microbial population. In finishing pigs, supplemental CPB was effective to improve ADG and nutrient digestibility but to decrease fecal noxious gas emission.

• Journal of Animal Science and Technology
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• v.48 no.4
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• pp.503-512
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• 2006

This study was conducted to investigate the effects of dietary Lactobacillus brevis (3.4×108 CFU/g) supplementation on growth performance, DM and N digestibilities, blood cell counts and fecal odor emission compounds in growing pigs. Ninety six crossbred [(Landrace×Yorkshire)×Duroc] pigs with an initial BW of 24.60±1.28kg were used for 42-d feeding trial according to a completely randomized design. Three corn- soybean meal based dietary treatments included: 1) CON (basal diet); 2) LB1 (basal diet + Lactobacillus brevis 0.2%) and 3) LB2 (basal diet+Lactobacillus brevis 0.4%). There were three dietary treatments with eight replicate pens per treatment and four pigs per pen. Through the entire experimental period, ADG, ADFI and gain/feed had no significant differences among treatments(P>0.05). Nitrogen digestibility was increased in LB1 and LB2 treatments compared to CON treatment (linear effect, P<0.05), however, DM digestibility had no significant difference among all the treatments (P>0.05). The WBC, RBC and lymphocyte concentrations in whole blood were not affected by treatments (P>0.05). Fecal NH3N and H2S concentrations were significant decreased in LB2 treatment compared to CON treatment (linear effect, P<0.05). Fecal VFA (acetic acid and propionic acid) concentration was also reduced in LB2 treatment compared to CON treatment (linear effect, P<0.05). In conclusion, Lactobacillus brevis (3.4×108 CFU/g) supplementation at the level of 0.4% can improve nitrogen digestibility and decrease the concentrations of fecal odor emission compounds in growing pigs.